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The map label for this gene is ubiE

Identifier: 66043656

GI number: 66043656

Start: 417587

End: 418357

Strand: Reverse

Name: ubiE

Synonym: Psyr_0389

Alternate gene names: 66043656

Gene position: 418357-417587 (Counterclockwise)

Preceding gene: 66043660

Following gene: 66043655

Centisome position: 6.87

GC content: 59.79

Gene sequence:

>771_bases
ATGAACGATCAGCGCAAAGGCAGCGATGCCGAACCTACGACTCACTTCGGTTACAAAAATGTACCGGAAAGCCAGAAGGC
AGAAAAAGTCGCTGAGGTTTTCCATTCGGTAGCGGCCAAGTACGACCTGATGAACGACCTGCTGTCCGGCGGCATGCATC
GCCTGTGGAAGCGTTTCGCGATCGAGCTGTCCGGCGTCCGCACCGGTAACCGCGTGCTGGACATTGCTGGCGGCACGGGC
GACCTGACCCGCAAATTCTCCAACCTGGTCGGCCCGACCGGCCAGGTGGTACTGGCTGACATCAACGCCTCCATGCTCAA
GGTCGGCCGTGACCGCCTGCTGGACCTGGGTGTGTCGGGCAACGTCGAGTTCGTCCAGGCAGACGCCGAGAAGCTGCCAT
TCCCGGACAACCATTTCGACTGCGTGACCATCGCGTTCGGCCTGCGCAACGTGACGCACAAGGAAGACGCCCTGCGTTCC
ATGCTGCGCGTGCTCAAGCCGGGTGGTCGCCTGTTGGTGCTGGAGTTCTCCAAGCCGACCAACAAGTTGATGTCCAAGGC
CTACGACGCCTACTCGTTCGCGTTCATGCCGTTGATGGGCAAGCTGGTCACCAATGATTCGGAAAGCTACCGCTATCTGG
CCGAATCGATCCGCATGCACCCCAACCAGGAAACCCTCAAGTCGATGATGGTAGAAGCCGGTTTTGACCGCGTGACCTAT
CACAACATGACTGCCGGCGTCGTGGCCCTGCACCGCGGTATCAAACCCTGA

Upstream 100 bases:

>100_bases
ACACCCGCCCGCATCCAAGAAAGCGTTGCAGGTGAGCCTTGTCAAGACAAAGCCGGCCTTGGCGATTAGAATGACCCGCA
CTCTATTCCGGTGAAGCGAT

Downstream 100 bases:

>100_bases
TGTTGCTCCGGGGGCTGCTCGCCAGCATCGAGCACGGCATCAATCGGGTGCTGCGCCTGGACAGCACCGCGCTGCCCCGC
ATGGCGCGTCTGTCCGGACG

Product: ubiquinone/menaquinone biosynthesis methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MNDQRKGSDAEPTTHFGYKNVPESQKAEKVAEVFHSVAAKYDLMNDLLSGGMHRLWKRFAIELSGVRTGNRVLDIAGGTG
DLTRKFSNLVGPTGQVVLADINASMLKVGRDRLLDLGVSGNVEFVQADAEKLPFPDNHFDCVTIAFGLRNVTHKEDALRS
MLRVLKPGGRLLVLEFSKPTNKLMSKAYDAYSFAFMPLMGKLVTNDSESYRYLAESIRMHPNQETLKSMMVEAGFDRVTY
HNMTAGVVALHRGIKP

Sequences:

>Translated_256_residues
MNDQRKGSDAEPTTHFGYKNVPESQKAEKVAEVFHSVAAKYDLMNDLLSGGMHRLWKRFAIELSGVRTGNRVLDIAGGTG
DLTRKFSNLVGPTGQVVLADINASMLKVGRDRLLDLGVSGNVEFVQADAEKLPFPDNHFDCVTIAFGLRNVTHKEDALRS
MLRVLKPGGRLLVLEFSKPTNKLMSKAYDAYSFAFMPLMGKLVTNDSESYRYLAESIRMHPNQETLKSMMVEAGFDRVTY
HNMTAGVVALHRGIKP
>Mature_256_residues
MNDQRKGSDAEPTTHFGYKNVPESQKAEKVAEVFHSVAAKYDLMNDLLSGGMHRLWKRFAIELSGVRTGNRVLDIAGGTG
DLTRKFSNLVGPTGQVVLADINASMLKVGRDRLLDLGVSGNVEFVQADAEKLPFPDNHFDCVTIAFGLRNVTHKEDALRS
MLRVLKPGGRLLVLEFSKPTNKLMSKAYDAYSFAFMPLMGKLVTNDSESYRYLAESIRMHPNQETLKSMMVEAGFDRVTY
HNMTAGVVALHRGIKP

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2)

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family

Homologues:

Organism=Homo sapiens, GI116063536, Length=275, Percent_Identity=44.7272727272727, Blast_Score=244, Evalue=4e-65,
Organism=Escherichia coli, GI48994983, Length=246, Percent_Identity=71.1382113821138, Blast_Score=371, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17557039, Length=259, Percent_Identity=44.7876447876448, Blast_Score=228, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6323526, Length=257, Percent_Identity=42.0233463035019, Blast_Score=182, Evalue=4e-47,
Organism=Drosophila melanogaster, GI24641702, Length=261, Percent_Identity=48.6590038314176, Blast_Score=248, Evalue=3e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UBIE_PSE14 (Q48PJ4)

Other databases:

- EMBL:   CP000058
- RefSeq:   YP_272675.1
- ProteinModelPortal:   Q48PJ4
- SMR:   Q48PJ4
- STRING:   Q48PJ4
- GeneID:   3556338
- GenomeReviews:   CP000058_GR
- KEGG:   psp:PSPPH_0372
- eggNOG:   COG2226
- HOGENOM:   HBG559580
- OMA:   FDVYTIA
- PhylomeDB:   Q48PJ4
- ProtClustDB:   PRK00216
- HAMAP:   MF_01813
- InterPro:   IPR004033
- PANTHER:   PTHR10108:SF24
- TIGRFAMs:   TIGR01934

Pfam domain/function: PF01209 Ubie_methyltran

EC number: 2.1.1.-

Molecular weight: Translated: 28423; Mature: 28423

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: PS01183 UBIE_1; PS01184 UBIE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDQRKGSDAEPTTHFGYKNVPESQKAEKVAEVFHSVAAKYDLMNDLLSGGMHRLWKRFA
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IELSGVRTGNRVLDIAGGTGDLTRKFSNLVGPTGQVVLADINASMLKVGRDRLLDLGVSG
HHHCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHEECCCCC
NVEFVQADAEKLPFPDNHFDCVTIAFGLRNVTHKEDALRSMLRVLKPGGRLLVLEFSKPT
CEEEEECCHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCCH
NKLMSKAYDAYSFAFMPLMGKLVTNDSESYRYLAESIRMHPNQETLKSMMVEAGFDRVTY
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHH
HNMTAGVVALHRGIKP
HHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNDQRKGSDAEPTTHFGYKNVPESQKAEKVAEVFHSVAAKYDLMNDLLSGGMHRLWKRFA
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IELSGVRTGNRVLDIAGGTGDLTRKFSNLVGPTGQVVLADINASMLKVGRDRLLDLGVSG
HHHCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHEECCCCC
NVEFVQADAEKLPFPDNHFDCVTIAFGLRNVTHKEDALRSMLRVLKPGGRLLVLEFSKPT
CEEEEECCHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCCH
NKLMSKAYDAYSFAFMPLMGKLVTNDSESYRYLAESIRMHPNQETLKSMMVEAGFDRVTY
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHH
HNMTAGVVALHRGIKP
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA