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The map label for this gene is flbD [H]

Identifier: 66043596

GI number: 66043596

Start: 345887

End: 346837

Strand: Direct

Name: flbD [H]

Synonym: Psyr_0327

Alternate gene names: 66043596

Gene position: 345887-346837 (Clockwise)

Preceding gene: 66043595

Following gene: 66043597

Centisome position: 5.68

GC content: 61.62

Gene sequence:

>951_bases
ATGAGCCTTCACGAAAACTTCGGCCAGCCGCTGCTGACCTTTCCCGATGCGGAAAAAAGCCCACTGAGCATTCGCGCCAA
GGCGCTGGTGTTCATCGATCCACGCTCGCGCCGGTTGCGTGAAGAAATGGAGCAACTGGCTCCCCGTTCTCTTCCGGTAC
TGATCCGTGGCGAGTCCGGGACCGGCAAGGAATTGCTGGCCCGGCACATTCATCGCGGCAGTGATCGCTCGGGTTTGTTC
GTTTCAGTGAACTGCGGTGCCATCAGCCCTACATACGCCGATGCCGAATTGTTCGGCTATGCCGCCGGAGCCCACAGTGG
GACGGTCAGCAGCCGCGCGGGATGGTTCGGTTCGGCCAATGGCGGCACCTTGTACCTGGACGAGATCGGCGACCTGCCGT
TGCCGATCCAGGTCAAGCTGCTGGCGGCCCTTGAGAATCACGAAGTCACGCGAGTGGGCGCGCAGCAGCCGAGCCCGGTC
GATGTGCGTCTGGTCGCCGCCACCAGTATCGATCTGGCGCAGGCCGTGGCTGCCGGGAAATTCCACGAGCGTCTTTATCA
TTACCTGAGCGAGGGCCGCCTCGATCTGCCTGCATTGCGCGAGCAGCCGGGCAACATCCTGCCGTTGGCCGAATATTTTG
TGGGGATCTACAGCCAGCGTCTGAACCTGCCGGTGCAACTGATCAGCGAGGAGGCGCAGCGGACGCTGGAGGCCCACAGC
TGGCCCGGCAATACCCGTGAACTGGAGAACGTCATTCACTTCGCCCTGCTGGTCAGCAGCGGTGATGAAATTCGCGCACA
GGATATCAATCTGCCGGACATCGCCGATCCGTTGACGCTGATCGAGCGCCAGACGCAGCTATTGCTCAACGGTAGTGATG
GCAGGCAGCTTTCCGCGCTCAGGCAATTGCTTGAAACAGTCACCTCGGAGCTGGCGCGGCGACCTGCTTGA

Upstream 100 bases:

>100_bases
CGCGTCGTCGCGCATGACCGGGCAGCTTGTTTATCGGACTCACCGGGTCCCGTCTTTTTAAACGCTTGAGCCATCGCAAG
GGCTCAGGCGGGAGTGTGTC

Downstream 100 bases:

>100_bases
GCGCGTCCCGGCGCGGTCTTGATGAGGTACAGTAGCTTCTGGTGCAAAAAAGCATAAGCACTCGATTAAAAAGTATTTTA
TCGGAATATAAAACACCGGT

Product: sigma-54 factor, interaction region

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MSLHENFGQPLLTFPDAEKSPLSIRAKALVFIDPRSRRLREEMEQLAPRSLPVLIRGESGTGKELLARHIHRGSDRSGLF
VSVNCGAISPTYADAELFGYAAGAHSGTVSSRAGWFGSANGGTLYLDEIGDLPLPIQVKLLAALENHEVTRVGAQQPSPV
DVRLVAATSIDLAQAVAAGKFHERLYHYLSEGRLDLPALREQPGNILPLAEYFVGIYSQRLNLPVQLISEEAQRTLEAHS
WPGNTRELENVIHFALLVSSGDEIRAQDINLPDIADPLTLIERQTQLLLNGSDGRQLSALRQLLETVTSELARRPA

Sequences:

>Translated_316_residues
MSLHENFGQPLLTFPDAEKSPLSIRAKALVFIDPRSRRLREEMEQLAPRSLPVLIRGESGTGKELLARHIHRGSDRSGLF
VSVNCGAISPTYADAELFGYAAGAHSGTVSSRAGWFGSANGGTLYLDEIGDLPLPIQVKLLAALENHEVTRVGAQQPSPV
DVRLVAATSIDLAQAVAAGKFHERLYHYLSEGRLDLPALREQPGNILPLAEYFVGIYSQRLNLPVQLISEEAQRTLEAHS
WPGNTRELENVIHFALLVSSGDEIRAQDINLPDIADPLTLIERQTQLLLNGSDGRQLSALRQLLETVTSELARRPA
>Mature_315_residues
SLHENFGQPLLTFPDAEKSPLSIRAKALVFIDPRSRRLREEMEQLAPRSLPVLIRGESGTGKELLARHIHRGSDRSGLFV
SVNCGAISPTYADAELFGYAAGAHSGTVSSRAGWFGSANGGTLYLDEIGDLPLPIQVKLLAALENHEVTRVGAQQPSPVD
VRLVAATSIDLAQAVAAGKFHERLYHYLSEGRLDLPALREQPGNILPLAEYFVGIYSQRLNLPVQLISEEAQRTLEAHSW
PGNTRELENVIHFALLVSSGDEIRAQDINLPDIADPLTLIERQTQLLLNGSDGRQLSALRQLLETVTSELARRPA

Specific function: Activation of sigma-54-dependent flagellar gene promoters and strong negative autoregulatory effects on its own promoter. The synthesis and function of flbD in C.crescentus is controlled by an internal cell-cycle clock [H]

COG id: COG1221

COG function: function code KT; Transcriptional regulators containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1786524, Length=241, Percent_Identity=44.8132780082988, Blast_Score=187, Evalue=7e-49,
Organism=Escherichia coli, GI87082152, Length=262, Percent_Identity=40.8396946564886, Blast_Score=187, Evalue=9e-49,
Organism=Escherichia coli, GI1790437, Length=311, Percent_Identity=37.9421221864952, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1788905, Length=269, Percent_Identity=40.5204460966543, Blast_Score=184, Evalue=8e-48,
Organism=Escherichia coli, GI87082117, Length=275, Percent_Identity=41.0909090909091, Blast_Score=184, Evalue=8e-48,
Organism=Escherichia coli, GI1790299, Length=226, Percent_Identity=42.9203539823009, Blast_Score=183, Evalue=1e-47,
Organism=Escherichia coli, GI1789233, Length=231, Percent_Identity=41.991341991342, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1788550, Length=228, Percent_Identity=44.7368421052632, Blast_Score=178, Evalue=3e-46,
Organism=Escherichia coli, GI1789087, Length=238, Percent_Identity=42.0168067226891, Blast_Score=175, Evalue=4e-45,
Organism=Escherichia coli, GI87081872, Length=240, Percent_Identity=42.0833333333333, Blast_Score=173, Evalue=1e-44,
Organism=Escherichia coli, GI1787583, Length=246, Percent_Identity=38.2113821138211, Blast_Score=173, Evalue=1e-44,
Organism=Escherichia coli, GI1789828, Length=260, Percent_Identity=35, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI87081858, Length=239, Percent_Identity=34.3096234309623, Blast_Score=123, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 34571; Mature: 34439

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLHENFGQPLLTFPDAEKSPLSIRAKALVFIDPRSRRLREEMEQLAPRSLPVLIRGESG
CCCCCHHCCCEEECCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHCCCCCCEEEECCCC
TGKELLARHIHRGSDRSGLFVSVNCGAISPTYADAELFGYAAGAHSGTVSSRAGWFGSAN
CCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHEECCCCCCCCCCCCCCCCCCC
GGTLYLDEIGDLPLPIQVKLLAALENHEVTRVGAQQPSPVDVRLVAATSIDLAQAVAAGK
CCEEEEECCCCCCCCHHHHEEEHHCCCCEEEECCCCCCCCEEEEEEECCHHHHHHHHHHH
FHERLYHYLSEGRLDLPALREQPGNILPLAEYFVGIYSQRLNLPVQLISEEAQRTLEAHS
HHHHHHHHHHCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
WPGNTRELENVIHFALLVSSGDEIRAQDINLPDIADPLTLIERQTQLLLNGSDGRQLSAL
CCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHEEEECCCCHHHHHH
RQLLETVTSELARRPA
HHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SLHENFGQPLLTFPDAEKSPLSIRAKALVFIDPRSRRLREEMEQLAPRSLPVLIRGESG
CCCCHHCCCEEECCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHCCCCCCEEEECCCC
TGKELLARHIHRGSDRSGLFVSVNCGAISPTYADAELFGYAAGAHSGTVSSRAGWFGSAN
CCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHEECCCCCCCCCCCCCCCCCCC
GGTLYLDEIGDLPLPIQVKLLAALENHEVTRVGAQQPSPVDVRLVAATSIDLAQAVAAGK
CCEEEEECCCCCCCCHHHHEEEHHCCCCEEEECCCCCCCCEEEEEEECCHHHHHHHHHHH
FHERLYHYLSEGRLDLPALREQPGNILPLAEYFVGIYSQRLNLPVQLISEEAQRTLEAHS
HHHHHHHHHHCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
WPGNTRELENVIHFALLVSSGDEIRAQDINLPDIADPLTLIERQTQLLLNGSDGRQLSAL
CCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHEEEECCCCHHHHHH
RQLLETVTSELARRPA
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2315326; 11259647 [H]