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The map label for this gene is pys2 [H]

Identifier: 66043580

GI number: 66043580

Start: 329734

End: 331704

Strand: Reverse

Name: pys2 [H]

Synonym: Psyr_0310

Alternate gene names: 66043580

Gene position: 331704-329734 (Counterclockwise)

Preceding gene: 66043581

Following gene: 66043579

Centisome position: 5.44

GC content: 53.48

Gene sequence:

>1971_bases
ATGGCAGATGAGCAAGAAAAACATATATATGTAAAATACTGGCCTATTCCAGACTTAGGGTCCGCAGACCGGGCAGCGGC
CGAGGCAGCTGCTAATAAAGCGAGTGGTGGAGGTAATTCAGGCTGGAGCATTGGAATCGGTGGTGGCGGCGGCGGACCCA
GCACTACCGGCCTAGGCCCAGGGCGCGGTCGCCGAAGAGGTATCACTAATAAACAGCTGAGACGTCTATTAAGGCAACAA
GAGGCAGAGGCGCATGCGTTGGCCCAGCGGCAAGCGGCAGAACACGCGGCTCATCTCGCAAGACTAGAAGCTGAGGCGCA
TGCTCGGGCAATGGCGGAAGCCGCCGAGCGAGAGCGCGTTGAAACGCAGGTGCGCGCACAGGCCGAAAAAGATCAGGCAC
ATCGACAGGCTCTTGAAACGCTGACAGCGTCCTATCCCTCGTTACAATCTGGATTGGCACAAAACTACTTACTGGCTTCT
GCATCGCTGCCAAATGATCTTCAAAAAGAAATAGCGTCAGGTCATGATCTAAATGCTGATCCCCCACGCGACGAACAGGC
TCTTGAGCTGATACTTCAGAAAAAAATCAGGGTTAACTATTTACTTGCAATCAAACAACCGTTACTGGAAGAGCGTCGCG
CTCAGGCTCTGAGTCTCACCGGTCAAGAGCTTGACCATGCTACACAAAAAGACCACCTGAACTATCTGGTGTACTACAGC
CAGGGTGATCCTCCACGTGTGCAGCAGGCCCATGAAGCGTGGATACAGGCGTTATCTCAGACCTACGAAGCAAAGCTTCT
TGCCGAATCGGTCACGCTGCTCAACGAACAATCAGCAGCGCTTTCCATGCGCCATGCTGAACTGTCGCTGGCCAATAAAC
CGGCGAGTCAGGATGCACGACAGGCCGCTGGCATCGACAAGCTGTGGTCAGTTATTGCCCCCGCGTCAACCACCACCGCA
GCAACAGGCATCAGAACCGTCGCGACCAACATCGCGAAGGATCAACTCATCAGGATTGCAACCAGAACACTGGGCAGCAA
TCTGGTTACGTTGCTGGCGATGTATCCACAACCGTTGGGCGACGCAGAACTGCCGCCCGCCGTTATCGCCACACCTCTGT
CGCAGCTCAACCTGCCGCCGCATATCGATCTGCACTACCTTGCCAGCGTCAAAGGCACGCTGGATGTGCCTCATCGACTG
ACATCCGATGAAGCTGGCACAAGCGGCGCTGCACGCTGGGTAGCTACGGACGGTGTGGAAGTTGGTACCAAGGTTCGCGT
AAGAACTTTCACCTATAACGCACAGAACAATTCCTACGAGTTTATCCGTGACGGGGAATCGACACCGGCACTGATATGGA
CGCCAATAGCACGACCGGCGGACAGCTCGACAAGCTCACCCGCAGGACCACCCGCTTTGCCGGTAGATCCAGGCAACGTG
GTGACACCTTTTGTACCCGAGCTTGAAGCTTACCCGGCGATTGATCGAGATGATCCTGATGATTACATTCTCATATCGCC
GATTGATTCAGGGCTGCCGAATAGCTACCTGCTGTTCAAGGACCCGAGGTCGATTCCGGGGGTTGCGAGTGGGTATGGTG
AAGCGGTCAATGGAGTTTGGCTAGGGGACAAAACTCGGGCAGAGGGCGCATCAATTCCGGCGCATATCGCAGATCAATTG
CGTGGACGACGATTTGGTAATTTTGACTCACTGAGGAAAGCAACTTGGATAGCCGTAGCCAATGATCCCGAGTTAGTTAA
ACAGTTTACTCAACATAACCTTGAAATAATGCGAGATGGCGGTGCGCCATACCCAAGGCTAGTCGACCAAGCTGGGGGCC
GTACAAAATTTGAAATCCACCATAAAAAACATATTGCAAACGGAGGTGCAGTGTACGACATCGACAACTTGGTTATCATG
ACCCCAAGACAACATATTGACCATCACAGGAGCCATGAGAATGATCTTTAA

Upstream 100 bases:

>100_bases
AACACTCGTAATACAGTATGAAGCAAAAAATATTTATGTATCACAATGACGGAGAAAAAGCGGATTAATTAGTCGCCATA
CAGCTAATAAGGAGAACTGT

Downstream 100 bases:

>100_bases
AGAAAAGATAGAAGATTATACCGAAGAAGAGTTCCTGGAGTTTTTGAAAGGGCTCTCTTCCGAATACAGCCAACTTCATG
GAGATGAGTTTATTAAACAT

Product: HNH endonuclease:S-type Pyocin

Products: NA

Alternate protein names: Killer protein [H]

Number of amino acids: Translated: 656; Mature: 655

Protein sequence:

>656_residues
MADEQEKHIYVKYWPIPDLGSADRAAAEAAANKASGGGNSGWSIGIGGGGGGPSTTGLGPGRGRRRGITNKQLRRLLRQQ
EAEAHALAQRQAAEHAAHLARLEAEAHARAMAEAAERERVETQVRAQAEKDQAHRQALETLTASYPSLQSGLAQNYLLAS
ASLPNDLQKEIASGHDLNADPPRDEQALELILQKKIRVNYLLAIKQPLLEERRAQALSLTGQELDHATQKDHLNYLVYYS
QGDPPRVQQAHEAWIQALSQTYEAKLLAESVTLLNEQSAALSMRHAELSLANKPASQDARQAAGIDKLWSVIAPASTTTA
ATGIRTVATNIAKDQLIRIATRTLGSNLVTLLAMYPQPLGDAELPPAVIATPLSQLNLPPHIDLHYLASVKGTLDVPHRL
TSDEAGTSGAARWVATDGVEVGTKVRVRTFTYNAQNNSYEFIRDGESTPALIWTPIARPADSSTSSPAGPPALPVDPGNV
VTPFVPELEAYPAIDRDDPDDYILISPIDSGLPNSYLLFKDPRSIPGVASGYGEAVNGVWLGDKTRAEGASIPAHIADQL
RGRRFGNFDSLRKATWIAVANDPELVKQFTQHNLEIMRDGGAPYPRLVDQAGGRTKFEIHHKKHIANGGAVYDIDNLVIM
TPRQHIDHHRSHENDL

Sequences:

>Translated_656_residues
MADEQEKHIYVKYWPIPDLGSADRAAAEAAANKASGGGNSGWSIGIGGGGGGPSTTGLGPGRGRRRGITNKQLRRLLRQQ
EAEAHALAQRQAAEHAAHLARLEAEAHARAMAEAAERERVETQVRAQAEKDQAHRQALETLTASYPSLQSGLAQNYLLAS
ASLPNDLQKEIASGHDLNADPPRDEQALELILQKKIRVNYLLAIKQPLLEERRAQALSLTGQELDHATQKDHLNYLVYYS
QGDPPRVQQAHEAWIQALSQTYEAKLLAESVTLLNEQSAALSMRHAELSLANKPASQDARQAAGIDKLWSVIAPASTTTA
ATGIRTVATNIAKDQLIRIATRTLGSNLVTLLAMYPQPLGDAELPPAVIATPLSQLNLPPHIDLHYLASVKGTLDVPHRL
TSDEAGTSGAARWVATDGVEVGTKVRVRTFTYNAQNNSYEFIRDGESTPALIWTPIARPADSSTSSPAGPPALPVDPGNV
VTPFVPELEAYPAIDRDDPDDYILISPIDSGLPNSYLLFKDPRSIPGVASGYGEAVNGVWLGDKTRAEGASIPAHIADQL
RGRRFGNFDSLRKATWIAVANDPELVKQFTQHNLEIMRDGGAPYPRLVDQAGGRTKFEIHHKKHIANGGAVYDIDNLVIM
TPRQHIDHHRSHENDL
>Mature_655_residues
ADEQEKHIYVKYWPIPDLGSADRAAAEAAANKASGGGNSGWSIGIGGGGGGPSTTGLGPGRGRRRGITNKQLRRLLRQQE
AEAHALAQRQAAEHAAHLARLEAEAHARAMAEAAERERVETQVRAQAEKDQAHRQALETLTASYPSLQSGLAQNYLLASA
SLPNDLQKEIASGHDLNADPPRDEQALELILQKKIRVNYLLAIKQPLLEERRAQALSLTGQELDHATQKDHLNYLVYYSQ
GDPPRVQQAHEAWIQALSQTYEAKLLAESVTLLNEQSAALSMRHAELSLANKPASQDARQAAGIDKLWSVIAPASTTTAA
TGIRTVATNIAKDQLIRIATRTLGSNLVTLLAMYPQPLGDAELPPAVIATPLSQLNLPPHIDLHYLASVKGTLDVPHRLT
SDEAGTSGAARWVATDGVEVGTKVRVRTFTYNAQNNSYEFIRDGESTPALIWTPIARPADSSTSSPAGPPALPVDPGNVV
TPFVPELEAYPAIDRDDPDDYILISPIDSGLPNSYLLFKDPRSIPGVASGYGEAVNGVWLGDKTRAEGASIPAHIADQLR
GRRFGNFDSLRKATWIAVANDPELVKQFTQHNLEIMRDGGAPYPRLVDQAGGRTKFEIHHKKHIANGGAVYDIDNLVIMT
PRQHIDHHRSHENDL

Specific function: Causes breakdown of chromosomal DNA as well as complete inhibition of lipid synthesis in sensitive cells [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the colicin/pyosin nuclease family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003615
- InterPro:   IPR003060
- InterPro:   IPR010715
- InterPro:   IPR016128 [H]

Pfam domain/function: PF06958 Pyocin_S [H]

EC number: NA

Molecular weight: Translated: 71105; Mature: 70974

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADEQEKHIYVKYWPIPDLGSADRAAAEAAANKASGGGNSGWSIGIGGGGGGPSTTGLGP
CCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCC
GRGRRRGITNKQLRRLLRQQEAEAHALAQRQAAEHAAHLARLEAEAHARAMAEAAERERV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETQVRAQAEKDQAHRQALETLTASYPSLQSGLAQNYLLASASLPNDLQKEIASGHDLNAD
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEECCCCCHHHHHHHHCCCCCCCC
PPRDEQALELILQKKIRVNYLLAIKQPLLEERRAQALSLTGQELDHATQKDHLNYLVYYS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEC
QGDPPRVQQAHEAWIQALSQTYEAKLLAESVTLLNEQSAALSMRHAELSLANKPASQDAR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHH
QAAGIDKLWSVIAPASTTTAATGIRTVATNIAKDQLIRIATRTLGSNLVTLLAMYPQPLG
HHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DAELPPAVIATPLSQLNLPPHIDLHYLASVKGTLDVPHRLTSDEAGTSGAARWVATDGVE
CCCCCCHHHHCCHHHCCCCCCCCHHEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCCC
VGTKVRVRTFTYNAQNNSYEFIRDGESTPALIWTPIARPADSSTSSPAGPPALPVDPGNV
CCCEEEEEEEEECCCCCCCCEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
VTPFVPELEAYPAIDRDDPDDYILISPIDSGLPNSYLLFKDPRSIPGVASGYGEAVNGVW
CCCCCCCHHCCCCCCCCCCCCEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCHHCCEEE
LGDKTRAEGASIPAHIADQLRGRRFGNFDSLRKATWIAVANDPELVKQFTQHNLEIMRDG
ECCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHEEEEEECCCHHHHHHHHHCCEEEEECC
GAPYPRLVDQAGGRTKFEIHHKKHIANGGAVYDIDNLVIMTPRQHIDHHRSHENDL
CCCCHHHHHCCCCCEEEEEEHHHHCCCCCCEEEECCEEEECCHHHHHHHCCCCCCC
>Mature Secondary Structure 
ADEQEKHIYVKYWPIPDLGSADRAAAEAAANKASGGGNSGWSIGIGGGGGGPSTTGLGP
CCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCC
GRGRRRGITNKQLRRLLRQQEAEAHALAQRQAAEHAAHLARLEAEAHARAMAEAAERERV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETQVRAQAEKDQAHRQALETLTASYPSLQSGLAQNYLLASASLPNDLQKEIASGHDLNAD
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEECCCCCHHHHHHHHCCCCCCCC
PPRDEQALELILQKKIRVNYLLAIKQPLLEERRAQALSLTGQELDHATQKDHLNYLVYYS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEC
QGDPPRVQQAHEAWIQALSQTYEAKLLAESVTLLNEQSAALSMRHAELSLANKPASQDAR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHH
QAAGIDKLWSVIAPASTTTAATGIRTVATNIAKDQLIRIATRTLGSNLVTLLAMYPQPLG
HHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DAELPPAVIATPLSQLNLPPHIDLHYLASVKGTLDVPHRLTSDEAGTSGAARWVATDGVE
CCCCCCHHHHCCHHHCCCCCCCCHHEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCCC
VGTKVRVRTFTYNAQNNSYEFIRDGESTPALIWTPIARPADSSTSSPAGPPALPVDPGNV
CCCEEEEEEEEECCCCCCCCEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
VTPFVPELEAYPAIDRDDPDDYILISPIDSGLPNSYLLFKDPRSIPGVASGYGEAVNGVW
CCCCCCCHHCCCCCCCCCCCCEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCHHCCEEE
LGDKTRAEGASIPAHIADQLRGRRFGNFDSLRKATWIAVANDPELVKQFTQHNLEIMRDG
ECCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHEEEEEECCCHHHHHHHHHCCEEEEECC
GAPYPRLVDQAGGRTKFEIHHKKHIANGGAVYDIDNLVIMTPRQHIDHHRSHENDL
CCCCHHHHHCCCCCEEEEEEHHHHCCCCCCEEEECCEEEECCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8491711; 10984043 [H]