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The map label for this gene is puuR [H]

Identifier: 66043508

GI number: 66043508

Start: 251961

End: 252560

Strand: Direct

Name: puuR [H]

Synonym: Psyr_0238

Alternate gene names: 66043508

Gene position: 251961-252560 (Clockwise)

Preceding gene: 66043507

Following gene: 66043509

Centisome position: 4.13

GC content: 57.67

Gene sequence:

>600_bases
ATGATGGCGTTCACATCGACTAGACCTGACAAAACCTGGGGGAATATCGCATTGGACGTGGGTGAACGACTGCAATCGAT
TCGCAAGCTCAAAGGCTTGTCCCAGCGTGAACTCGCCAAGCGCGCAGGTGTGACCAACAGCACCATCTCCATGATCGAAA
AAAACAGCGTCAGCCCTTCGATCAGTTCGCTGCGCAAGGTGCTCGGCGGCATCCCCATGTCCATGGTCGAATTTTTCTCG
GAAGAGTCCGTGCCCGAAAACGCGGCTCAAGTGGTCTACAAGGCCAGCGAGCTGATTGATATATCGGACGGTGCCGTGAC
CATGAAGCTGGTCGGCAAATCGCATCAGGGCCGTGCCATCGCCTTTCTCACCGAAACCTATCCACCGGGTGCCGATACCG
GCGAAGAGATGCTCGTCCACGAAGGCGAAGAGACCGGCATCCTCGTCGAGGGGCGGCTGGAACTGGTGGTCGGGCTGGAT
ACCTACGTGCTCGAAGCGGGTGACAGCTATTATTTCGAAAGCACCCGGCCGCATCGCTTTCGTAACCCGTTCGACGTCCC
GGCACGATTGATCAGTGCGGCCACCCCGGCCAATTTCTGA

Upstream 100 bases:

>100_bases
TTCCGCGCATCTATTCCGAGGACTGATTCAGCCCTCTGTGCTCCAGGCCGCGGTGGGTCAGGTGTCTGTCGAAGTGTTGT
AAATATCGAACGCTGTTGCC

Downstream 100 bases:

>100_bases
CCCGCCCCCTTGCGATGAGTGACGCGTGAAACTGACCTGGAAGATTCTGAGCAGCGCAGGCGTTCTGACGTTGTGGGCGC
TCAATGCGCAAGCCGCCGAT

Product: helix-hairpin-helix DNA-binding motif-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MMAFTSTRPDKTWGNIALDVGERLQSIRKLKGLSQRELAKRAGVTNSTISMIEKNSVSPSISSLRKVLGGIPMSMVEFFS
EESVPENAAQVVYKASELIDISDGAVTMKLVGKSHQGRAIAFLTETYPPGADTGEEMLVHEGEETGILVEGRLELVVGLD
TYVLEAGDSYYFESTRPHRFRNPFDVPARLISAATPANF

Sequences:

>Translated_199_residues
MMAFTSTRPDKTWGNIALDVGERLQSIRKLKGLSQRELAKRAGVTNSTISMIEKNSVSPSISSLRKVLGGIPMSMVEFFS
EESVPENAAQVVYKASELIDISDGAVTMKLVGKSHQGRAIAFLTETYPPGADTGEEMLVHEGEETGILVEGRLELVVGLD
TYVLEAGDSYYFESTRPHRFRNPFDVPARLISAATPANF
>Mature_199_residues
MMAFTSTRPDKTWGNIALDVGERLQSIRKLKGLSQRELAKRAGVTNSTISMIEKNSVSPSISSLRKVLGGIPMSMVEFFS
EESVPENAAQVVYKASELIDISDGAVTMKLVGKSHQGRAIAFLTETYPPGADTGEEMLVHEGEETGILVEGRLELVVGLD
TYVLEAGDSYYFESTRPHRFRNPFDVPARLISAATPANF

Specific function: Represses puuD [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787557, Length=183, Percent_Identity=43.1693989071038, Blast_Score=139, Evalue=2e-34,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013096
- InterPro:   IPR011051
- InterPro:   IPR001387
- InterPro:   IPR010982
- InterPro:   IPR014710 [H]

Pfam domain/function: PF07883 Cupin_2; PF01381 HTH_3 [H]

EC number: NA

Molecular weight: Translated: 21742; Mature: 21742

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMAFTSTRPDKTWGNIALDVGERLQSIRKLKGLSQRELAKRAGVTNSTISMIEKNSVSPS
CCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHCCCCCCC
ISSLRKVLGGIPMSMVEFFSEESVPENAAQVVYKASELIDISDGAVTMKLVGKSHQGRAI
HHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHEEECCCCEEEEEEECCCCCCCEE
AFLTETYPPGADTGEEMLVHEGEETGILVEGRLELVVGLDTYVLEAGDSYYFESTRPHRF
EEEEECCCCCCCCCHHHHEECCCCCCEEEECCEEEEECCCEEEEECCCCEEECCCCCCCC
RNPFDVPARLISAATPANF
CCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MMAFTSTRPDKTWGNIALDVGERLQSIRKLKGLSQRELAKRAGVTNSTISMIEKNSVSPS
CCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHCCCCCCC
ISSLRKVLGGIPMSMVEFFSEESVPENAAQVVYKASELIDISDGAVTMKLVGKSHQGRAI
HHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHEEECCCCEEEEEEECCCCCCCEE
AFLTETYPPGADTGEEMLVHEGEETGILVEGRLELVVGLDTYVLEAGDSYYFESTRPHRF
EEEEECCCCCCCCCHHHHEECCCCCCEEEECCEEEEECCCEEEEECCCCEEECCCCCCCC
RNPFDVPARLISAATPANF
CCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553 [H]