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The map label for this gene is pckA [H]

Identifier: 66043438

GI number: 66043438

Start: 182216

End: 183760

Strand: Reverse

Name: pckA [H]

Synonym: Psyr_0168

Alternate gene names: 66043438

Gene position: 183760-182216 (Counterclockwise)

Preceding gene: 66043439

Following gene: 66043435

Centisome position: 3.02

GC content: 60.84

Gene sequence:

>1545_bases
ATGACGCAAACCAACAACGCTGTGTACACCGATCTGAGCACCGACGAACTGGTCAAGGAAGCCCTTGCCCGCAAAGAAGG
CGTACTTTCCGATACTGGCGCACTGGTTGTGGAGACAGGCCATCGTACTGGCCGTTCGCCGGTCGATCGTTTCATCGTTG
AAGAGCCGAGCACTCAGGACGCCATCGCCTGGGGCCCGATCAACCGCAAGTTCCCGGCCGAGAAATTCGACGCCCTGTGG
AACCGTGTCGGCGCCTACCTCGCCGAGCGCGAGCGCTTCGTTTCCCACGTTCATGTAGGCTCCGATCCTGCGCACTACCT
GCCAGTCAAGATGACCACCGAGACTGCGTGGCACAATCTGTTCGGCCGTTGCCTGTTCATCAACCCTGAACAGTACAACC
CGGCTGGCAAGGACGAGTGGGAAATCCAGAACGCGCCATGGTTCGTCTGCGACCCTGAGCGTGACGGCACCAACTCCGAC
GGCACCGTAATCATCAACTTCGCGGCCCGCAAGGTGCTGATCGCCGGCATGCGTTATGCCGGCGAAATGAAGAAAGCCAT
GTTCTCGGTGCAGAACTTCCTGCTCCCGGCGTCCGACGTTCTGCCGATGCACTGCGCGGCCAACATGGGCGAAGAGGGCG
ATGTAACCCTGTTCTTCGGTCTGTCCGGCACCGGCAAGACCACCCTGTCCGCCGACGAAAGCCGTTACCTGATCGGTGAC
GACGAACACGGCTGGGGCGAAGGCGTGGTGTTCAACATCGAAGGCGGTTGCTATGCCAAGTGCATCGACCTGTCCGAGAA
GAACGAGCCGGTCATCTGGAAAGCCATCCAGCACGGCGCCGTACTGGAAAACGTCGTACTCGACCCGGTCACCAAAAAAG
CCGACTACGCCGACAGCAGCCTGACCCAGAACAGCCGTGCGGCCTACCCGCGCGAACTGATCGAAAAACGCGCACCGAAA
AACCTCGGTGGCGAGCCGAACGCCGTTATCTTCCTGACCTGCGACCTGACCGGCGTACTGCCGCCTGTGTCGATCCTCAG
CGAAGAACAGGCTGCCTACCACTTCCTGTCGGGTTACACCGCACTGGTCGGCTCGACTGAAATGGGTTCGGGTTCGGGCA
TCAAGTCCACCTTCTCCACCTGCTTCGGCGCACCGTTCTTCCCGCGTCCGGCTGGCGAATACGCAGAGCTGCTGATCAAG
CGCATCCGCGGCTTCAAATCCAAGGTCTACCTGGTCAACACCGGCTGGACCGGCGGTGGCTACGGCGTCGGCAAGCGCTT
CAACATCCCGACCACCCGTGGCGTGATCGCAGCGATCCAGAGCGGCGCACTGATCGGTGCCGAAACCGAACACCTCGACA
CCATCAACCTCGACGTACCGAAGTCGGTACCCGGCGTCGATACCGGCCTGCTCAACCCACGCAACACCTGGGCTGACAAG
GCTGCCTACGACGAAGCGGCAAAAGCACTGGCTGGCCTGTTCGTGGAGAACTTCAAGAAGTTCGACGTGAGCGACGCCAT
CAAGGCTGCGGGTCCGAAGTTGTAA

Upstream 100 bases:

>100_bases
ATTTCTGGCATAATCCGGCCCACTTTTACGCTGTAGTAGTGGTTGTTTTTTTACTACAAAACCGTTTGGAGCACTCGGCC
ACAGGCCGACGGGGAACCTC

Downstream 100 bases:

>100_bases
GACGTTGTAATTAGCCTCCACTAAAAAAACCGCCTTCATCAGGCGGTTTTTTTTGTACTCGTTTTACGAATCTGAAAGCC
TGTTGCCTATCTTGCCAGAT

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MTQTNNAVYTDLSTDELVKEALARKEGVLSDTGALVVETGHRTGRSPVDRFIVEEPSTQDAIAWGPINRKFPAEKFDALW
NRVGAYLAERERFVSHVHVGSDPAHYLPVKMTTETAWHNLFGRCLFINPEQYNPAGKDEWEIQNAPWFVCDPERDGTNSD
GTVIINFAARKVLIAGMRYAGEMKKAMFSVQNFLLPASDVLPMHCAANMGEEGDVTLFFGLSGTGKTTLSADESRYLIGD
DEHGWGEGVVFNIEGGCYAKCIDLSEKNEPVIWKAIQHGAVLENVVLDPVTKKADYADSSLTQNSRAAYPRELIEKRAPK
NLGGEPNAVIFLTCDLTGVLPPVSILSEEQAAYHFLSGYTALVGSTEMGSGSGIKSTFSTCFGAPFFPRPAGEYAELLIK
RIRGFKSKVYLVNTGWTGGGYGVGKRFNIPTTRGVIAAIQSGALIGAETEHLDTINLDVPKSVPGVDTGLLNPRNTWADK
AAYDEAAKALAGLFVENFKKFDVSDAIKAAGPKL

Sequences:

>Translated_514_residues
MTQTNNAVYTDLSTDELVKEALARKEGVLSDTGALVVETGHRTGRSPVDRFIVEEPSTQDAIAWGPINRKFPAEKFDALW
NRVGAYLAERERFVSHVHVGSDPAHYLPVKMTTETAWHNLFGRCLFINPEQYNPAGKDEWEIQNAPWFVCDPERDGTNSD
GTVIINFAARKVLIAGMRYAGEMKKAMFSVQNFLLPASDVLPMHCAANMGEEGDVTLFFGLSGTGKTTLSADESRYLIGD
DEHGWGEGVVFNIEGGCYAKCIDLSEKNEPVIWKAIQHGAVLENVVLDPVTKKADYADSSLTQNSRAAYPRELIEKRAPK
NLGGEPNAVIFLTCDLTGVLPPVSILSEEQAAYHFLSGYTALVGSTEMGSGSGIKSTFSTCFGAPFFPRPAGEYAELLIK
RIRGFKSKVYLVNTGWTGGGYGVGKRFNIPTTRGVIAAIQSGALIGAETEHLDTINLDVPKSVPGVDTGLLNPRNTWADK
AAYDEAAKALAGLFVENFKKFDVSDAIKAAGPKL
>Mature_513_residues
TQTNNAVYTDLSTDELVKEALARKEGVLSDTGALVVETGHRTGRSPVDRFIVEEPSTQDAIAWGPINRKFPAEKFDALWN
RVGAYLAERERFVSHVHVGSDPAHYLPVKMTTETAWHNLFGRCLFINPEQYNPAGKDEWEIQNAPWFVCDPERDGTNSDG
TVIINFAARKVLIAGMRYAGEMKKAMFSVQNFLLPASDVLPMHCAANMGEEGDVTLFFGLSGTGKTTLSADESRYLIGDD
EHGWGEGVVFNIEGGCYAKCIDLSEKNEPVIWKAIQHGAVLENVVLDPVTKKADYADSSLTQNSRAAYPRELIEKRAPKN
LGGEPNAVIFLTCDLTGVLPPVSILSEEQAAYHFLSGYTALVGSTEMGSGSGIKSTFSTCFGAPFFPRPAGEYAELLIKR
IRGFKSKVYLVNTGWTGGGYGVGKRFNIPTTRGVIAAIQSGALIGAETEHLDTINLDVPKSVPGVDTGLLNPRNTWADKA
AYDEAAKALAGLFVENFKKFDVSDAIKAAGPKL

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family [H]

Homologues:

Organism=Escherichia coli, GI1789807, Length=522, Percent_Identity=44.2528735632184, Blast_Score=397, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6322950, Length=508, Percent_Identity=43.503937007874, Blast_Score=403, Evalue=1e-113,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994 [H]

Pfam domain/function: PF01293 PEPCK_ATP [H]

EC number: =4.1.1.49 [H]

Molecular weight: Translated: 55857; Mature: 55725

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQTNNAVYTDLSTDELVKEALARKEGVLSDTGALVVETGHRTGRSPVDRFIVEEPSTQD
CCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHCCCCCCCC
AIAWGPINRKFPAEKFDALWNRVGAYLAERERFVSHVHVGSDPAHYLPVKMTTETAWHNL
CEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEEEEEHHHHHHHH
FGRCLFINPEQYNPAGKDEWEIQNAPWFVCDPERDGTNSDGTVIINFAARKVLIAGMRYA
CCEEEEECCHHCCCCCCCCCEECCCCEEEECCCCCCCCCCCEEEEEEHHHHHHHHHHHHH
GEMKKAMFSVQNFLLPASDVLPMHCAANMGEEGDVTLFFGLSGTGKTTLSADESRYLIGD
HHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCEEEEEEECCCCCEEECCCCCEEEECC
DEHGWGEGVVFNIEGGCYAKCIDLSEKNEPVIWKAIQHGAVLENVVLDPVTKKADYADSS
CCCCCCCEEEEEECCCCEEEEEECCCCCCCEEEEHHHCCHHHHHHHHCCCCCCCCCCCCH
LTQNSRAAYPRELIEKRAPKNLGGEPNAVIFLTCDLTGVLPPVSILSEEQAAYHFLSGYT
HCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCHHHHCCHHHHHHHHHCHH
ALVGSTEMGSGSGIKSTFSTCFGAPFFPRPAGEYAELLIKRIRGFKSKVYLVNTGWTGGG
HEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCC
YGVGKRFNIPTTRGVIAAIQSGALIGAETEHLDTINLDVPKSVPGVDTGLLNPRNTWADK
CCCCCEECCCCHHHHHHHHHCCCEECCCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHH
AAYDEAAKALAGLFVENFKKFDVSDAIKAAGPKL
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCC
>Mature Secondary Structure 
TQTNNAVYTDLSTDELVKEALARKEGVLSDTGALVVETGHRTGRSPVDRFIVEEPSTQD
CCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHCCCCCCCC
AIAWGPINRKFPAEKFDALWNRVGAYLAERERFVSHVHVGSDPAHYLPVKMTTETAWHNL
CEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEEEEEHHHHHHHH
FGRCLFINPEQYNPAGKDEWEIQNAPWFVCDPERDGTNSDGTVIINFAARKVLIAGMRYA
CCEEEEECCHHCCCCCCCCCEECCCCEEEECCCCCCCCCCCEEEEEEHHHHHHHHHHHHH
GEMKKAMFSVQNFLLPASDVLPMHCAANMGEEGDVTLFFGLSGTGKTTLSADESRYLIGD
HHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCEEEEEEECCCCCEEECCCCCEEEECC
DEHGWGEGVVFNIEGGCYAKCIDLSEKNEPVIWKAIQHGAVLENVVLDPVTKKADYADSS
CCCCCCCEEEEEECCCCEEEEEECCCCCCCEEEEHHHCCHHHHHHHHCCCCCCCCCCCCH
LTQNSRAAYPRELIEKRAPKNLGGEPNAVIFLTCDLTGVLPPVSILSEEQAAYHFLSGYT
HCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCHHHHCCHHHHHHHHHCHH
ALVGSTEMGSGSGIKSTFSTCFGAPFFPRPAGEYAELLIKRIRGFKSKVYLVNTGWTGGG
HEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCC
YGVGKRFNIPTTRGVIAAIQSGALIGAETEHLDTINLDVPKSVPGVDTGLLNPRNTWADK
CCCCCEECCCCHHHHHHHHHCCCEECCCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHH
AAYDEAAKALAGLFVENFKKFDVSDAIKAAGPKL
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA