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The map label for this gene is entA [C]

Identifier: 66043390

GI number: 66043390

Start: 130166

End: 130936

Strand: Direct

Name: entA [C]

Synonym: Psyr_0120

Alternate gene names: 66043390

Gene position: 130166-130936 (Clockwise)

Preceding gene: 66043386

Following gene: 66043392

Centisome position: 2.14

GC content: 58.5

Gene sequence:

>771_bases
ATGAGTTTGATACCGGAACTGAACAGCGACGCTAACGTGCTGATATGCGGAGCCAGCCGAGGTATCGGCCTGGCACTGTG
CGCGGCGTTGCTTGCTCGCGATGATGTGGCTCAGGTATGGGCGGTGGCGCGAAAAGCCAGCACCTCCACAGAACTGGCAA
CGCTTGCAGAGCAATATGGCCAACGCATCAAACGCGTCGACTGCGACGCGCGCAATGAACAATCCCTCGAAGCACTCGTG
AGCGAGACGCTTGATGGGTGCGATCATCTGCACCTGGTCATCAGTACGCTAGGTATTCTCCATCAGGACGGCGCAAAAGC
AGAAAAGGGCCTGGCACAACTGACACTTGCGAGCCTGCAAGCGAGTTTCGCGACCAACACCTTTGCACCGATCCTGCTGC
TCAAACACCTACTCCCGCTATTGCGCAAACAACCTTCAACCTTCGCGGCGCTCTCCGCGAGGGTTGGCTCTATTGGCGAT
AACCGCTTGGGTGGCTGGTACAGCTACAGAGCCAGCAAAGCAGCGCTCAACCAGTTGCTGCACACGGCCAGTATCGAATT
GAAACGCCTCAACCCGGCCTCTACTGTCCTGGCAATACACCCAGGCACGACTGACACGGAGCTGTCTCAGCCATTTCAGG
CGAACGTACCCGAAGGCCAACTGTTCGAACCGGCGTTCTCGGCGGAGCGCATCATTGAGGTGGTAGGCGCGCATGGACCG
GCCGACAGCGGGACCTTTTGGGCCTGGGACGACAAGCCGATTGTCTGGTGA

Upstream 100 bases:

>100_bases
TTTTCGTTGAACGCCCTATTGGTAAGCAACGGTCCACCTGAACATAATGTCTTGCCGCGAAGTCAAAAAACCACTTCAAC
CATGCTCCTCAAGGTGCCTT

Downstream 100 bases:

>100_bases
CTTGTTTCACTGGTGAGGATGAGATTGCTGTAAGCCTGGGTCGGTTTGTCTGGCGGGCTGCTTTCGGCCAGAAGCAGCCT
GTCACTTTTCAAGTCCTCCT

Product: Short-chain dehydrogenase/reductase SDR

Products: 2,3-Dihydroxybenzoate; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: C Factor Cell-Cell Signaling Protein; Oxidoreductase Short Chain Dehydrogenase/Reductase Family; Short Chain Dehydrogenase/Reductase Family Oxidoreductase; Short Chain Dehydrogenase; C Factor; Cell-Cell Signaling Protein C-Factor; CsgA C-Factor Signaling Protein; CsgA Rossman Fold Oxidoreductase; Dehydrogenase; Short-Chain Dehydrogenase/Reductase; Cell-Cell Signaling Protein CsgA; SDR Family Dehydrogenase/Reductase; Short-Chain Dehydrogenase/Reductase Family Protein; Short-Chain Dehydrogenase/Oxidoreductase; Glucose/Ribitol Dehydrogenase; 2-Methylthioadenine Synthetase; NAD Dependent Epimerase/Dehydratase Family; Oxidoreductase Short-Chain Dehydrogenase/Reductase Family; C-Factor; C Factor Cell Signaling Protein

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MSLIPELNSDANVLICGASRGIGLALCAALLARDDVAQVWAVARKASTSTELATLAEQYGQRIKRVDCDARNEQSLEALV
SETLDGCDHLHLVISTLGILHQDGAKAEKGLAQLTLASLQASFATNTFAPILLLKHLLPLLRKQPSTFAALSARVGSIGD
NRLGGWYSYRASKAALNQLLHTASIELKRLNPASTVLAIHPGTTDTELSQPFQANVPEGQLFEPAFSAERIIEVVGAHGP
ADSGTFWAWDDKPIVW

Sequences:

>Translated_256_residues
MSLIPELNSDANVLICGASRGIGLALCAALLARDDVAQVWAVARKASTSTELATLAEQYGQRIKRVDCDARNEQSLEALV
SETLDGCDHLHLVISTLGILHQDGAKAEKGLAQLTLASLQASFATNTFAPILLLKHLLPLLRKQPSTFAALSARVGSIGD
NRLGGWYSYRASKAALNQLLHTASIELKRLNPASTVLAIHPGTTDTELSQPFQANVPEGQLFEPAFSAERIIEVVGAHGP
ADSGTFWAWDDKPIVW
>Mature_255_residues
SLIPELNSDANVLICGASRGIGLALCAALLARDDVAQVWAVARKASTSTELATLAEQYGQRIKRVDCDARNEQSLEALVS
ETLDGCDHLHLVISTLGILHQDGAKAEKGLAQLTLASLQASFATNTFAPILLLKHLLPLLRKQPSTFAALSARVGSIGDN
RLGGWYSYRASKAALNQLLHTASIELKRLNPASTVLAIHPGTTDTELSQPFQANVPEGQLFEPAFSAERIIEVVGAHGPA
DSGTFWAWDDKPIVW

Specific function: Siderophore biosynthesis; enterobactin biosynthesis. [C]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Caenorhabditis elegans, GI72001658, Length=250, Percent_Identity=26.4, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI193207563, Length=198, Percent_Identity=28.7878787878788, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17559962, Length=214, Percent_Identity=28.9719626168224, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17559964, Length=198, Percent_Identity=28.2828282828283, Blast_Score=64, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6322227, Length=216, Percent_Identity=29.6296296296296, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6322226, Length=204, Percent_Identity=25.4901960784314, Blast_Score=72, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24640547, Length=265, Percent_Identity=29.811320754717, Blast_Score=88, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.3.1.28 [C]

Molecular weight: Translated: 27428; Mature: 27296

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLIPELNSDANVLICGASRGIGLALCAALLARDDVAQVWAVARKASTSTELATLAEQYG
CCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
QRIKRVDCDARNEQSLEALVSETLDGCDHLHLVISTLGILHQDGAKAEKGLAQLTLASLQ
HHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
ASFATNTFAPILLLKHLLPLLRKQPSTFAALSARVGSIGDNRLGGWYSYRASKAALNQLL
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
HTASIELKRLNPASTVLAIHPGTTDTELSQPFQANVPEGQLFEPAFSAERIIEVVGAHGP
HHHHHHEEECCCCCEEEEECCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
ADSGTFWAWDDKPIVW
CCCCCEEEECCCCCCC
>Mature Secondary Structure 
SLIPELNSDANVLICGASRGIGLALCAALLARDDVAQVWAVARKASTSTELATLAEQYG
CCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
QRIKRVDCDARNEQSLEALVSETLDGCDHLHLVISTLGILHQDGAKAEKGLAQLTLASLQ
HHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
ASFATNTFAPILLLKHLLPLLRKQPSTFAALSARVGSIGDNRLGGWYSYRASKAALNQLL
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
HTASIELKRLNPASTVLAIHPGTTDTELSQPFQANVPEGQLFEPAFSAERIIEVVGAHGP
HHHHHHEEECCCCCEEEEECCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
ADSGTFWAWDDKPIVW
CCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 5550 [C]

Specific activity: 66

Km value (mM): 0.26 {methyl-2,3-dehydro-3-hydroxybenzoate}} 0.2 {3-oxocyclohexane-1-carboxylic} 0.3 {2,3-dihydro-2,3-dihydroxybenzoate}} [C]

Substrates: 2,3-Dihydro-2,3-dihydroxybenzoate; Nicotinamide adenine dinucleotide [C]

Specific reaction: 2,3-Dihydro-2,3-dihydroxybenzoate + Nicotinamide adenine dinucleotide <==> 2,3-Dihydroxybenzoate + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA