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The map label for this gene is insH10 [H]

Identifier: 66043367

GI number: 66043367

Start: 106580

End: 107563

Strand: Reverse

Name: insH10 [H]

Synonym: Psyr_0096

Alternate gene names: 66043367

Gene position: 107563-106580 (Counterclockwise)

Preceding gene: 66043371

Following gene: 66043366

Centisome position: 1.77

GC content: 55.79

Gene sequence:

>984_bases
ATGATGAAACAGATGACCTTCGCCGACGCCGAATACGCCGGCAAGCGCAAGCGGACCCGTAAGGAATTGTTCCTGATCGA
GATGGATCAGGTGGTGCCCTGGAAAGGTTTGATTGCCCTGATTGAGCCCTATTATCCAAAAGGAGAAGGTGGCCGTCCCG
CCTACCCACTGATGGCGATGCTGCGTGTTCATCTGATGCAGAACTGGTTCGGCTACAGCGATCCGGCGATGGAAGAAGCA
CTGTATGAGACGACCATCCTGCGTCAGTTTTCAGGCCTGAGCCTGGAGCGCATTCCAGACGAAACCACCATTCTCAACTT
CCGTCGTCTGCTGGAAAAGCACGAGTTGGCGACTGGAATTCTCGGCGTAATTAATGGTTATCTGGGCGACCGTGGCTTGT
CGTTGCGTCAAGGCACCATCGTCGATGCCACGCTGATTCACGCGCCCAGTTCGACCAAGAACAAAGATGGCAAACGCGAC
CCTGAGATGCATCAAACCAAGAAAGGTAACCAGTACTATTTCGGCGCCAAGGCTCACATCGGCGCCGACGACGAGTCAGG
CCTGGTGCACAGCGTGGTAGTAACAGCGGCCAATGTGGCAGACGTCACGCAGGTTGCCAAACTGCTGCACGGCGAGGAAA
ACGTAGTCTGCGCCGACGCAGGTTACACCGGGGTAGAGAAGCGCGAAGAACATACTGGGCGCAAGGTCATTTGGCAGATT
GCCGCCCGCCGTAGCACTTACAAAAAACACGGGAAACGCAATGTTTTGTACAAGGCGATCCGCAAAATCGAGAAGGCCAA
GGCCCAGGTTCGCGCCAAGGTCGAGCATCCGTTTCGGGTAATCAAACGCCAGTTTGGCTATGAAAAAGTGCGCTTTCGGG
GCTTGGCCAAGAACACCGCGCAGATGGTGACGTTGTTCGCCCTGTCAAACCTGTGGATGGCCCGCCGACATTTGTTGGCG
AGCGCAGGAGAGGTGCGCGTGTAA

Upstream 100 bases:

>100_bases
GAAAAAGGGCCCGTCTCAGACTGTGTGAAAACCTAGGGAAACTCTGAAAAAGACTTCCTAATTTGGCAAAATCTCCGGAC
ACCAGTCGCCGAGCTTTTCG

Downstream 100 bases:

>100_bases
TGCAGGAAATGGTTACTGCAAAGGGCTTTGCAGCGGCCGAAAGAGCTGAAAAACGAAGAAAATTGAGCAAATTTCGACAT
GCATCGTTTTTTGAAAAGTG

Product: transposase IS4

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MMKQMTFADAEYAGKRKRTRKELFLIEMDQVVPWKGLIALIEPYYPKGEGGRPAYPLMAMLRVHLMQNWFGYSDPAMEEA
LYETTILRQFSGLSLERIPDETTILNFRRLLEKHELATGILGVINGYLGDRGLSLRQGTIVDATLIHAPSSTKNKDGKRD
PEMHQTKKGNQYYFGAKAHIGADDESGLVHSVVVTAANVADVTQVAKLLHGEENVVCADAGYTGVEKREEHTGRKVIWQI
AARRSTYKKHGKRNVLYKAIRKIEKAKAQVRAKVEHPFRVIKRQFGYEKVRFRGLAKNTAQMVTLFALSNLWMARRHLLA
SAGEVRV

Sequences:

>Translated_327_residues
MMKQMTFADAEYAGKRKRTRKELFLIEMDQVVPWKGLIALIEPYYPKGEGGRPAYPLMAMLRVHLMQNWFGYSDPAMEEA
LYETTILRQFSGLSLERIPDETTILNFRRLLEKHELATGILGVINGYLGDRGLSLRQGTIVDATLIHAPSSTKNKDGKRD
PEMHQTKKGNQYYFGAKAHIGADDESGLVHSVVVTAANVADVTQVAKLLHGEENVVCADAGYTGVEKREEHTGRKVIWQI
AARRSTYKKHGKRNVLYKAIRKIEKAKAQVRAKVEHPFRVIKRQFGYEKVRFRGLAKNTAQMVTLFALSNLWMARRHLLA
SAGEVRV
>Mature_327_residues
MMKQMTFADAEYAGKRKRTRKELFLIEMDQVVPWKGLIALIEPYYPKGEGGRPAYPLMAMLRVHLMQNWFGYSDPAMEEA
LYETTILRQFSGLSLERIPDETTILNFRRLLEKHELATGILGVINGYLGDRGLSLRQGTIVDATLIHAPSSTKNKDGKRD
PEMHQTKKGNQYYFGAKAHIGADDESGLVHSVVVTAANVADVTQVAKLLHGEENVVCADAGYTGVEKREEHTGRKVIWQI
AARRSTYKKHGKRNVLYKAIRKIEKAKAQVRAKVEHPFRVIKRQFGYEKVRFRGLAKNTAQMVTLFALSNLWMARRHLLA
SAGEVRV

Specific function: Involved in the transposition of the insertion sequence IS5 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 11 family [H]

Homologues:

Organism=Escherichia coli, GI1788303, Length=318, Percent_Identity=55.3459119496855, Blast_Score=365, Evalue=1e-102,
Organism=Escherichia coli, GI1789921, Length=318, Percent_Identity=55.0314465408805, Blast_Score=364, Evalue=1e-102,
Organism=Escherichia coli, GI1789612, Length=318, Percent_Identity=55.0314465408805, Blast_Score=364, Evalue=1e-102,
Organism=Escherichia coli, GI1789355, Length=318, Percent_Identity=55.0314465408805, Blast_Score=364, Evalue=1e-102,
Organism=Escherichia coli, GI1788518, Length=318, Percent_Identity=55.0314465408805, Blast_Score=364, Evalue=1e-102,
Organism=Escherichia coli, GI1788342, Length=318, Percent_Identity=55.0314465408805, Blast_Score=364, Evalue=1e-102,
Organism=Escherichia coli, GI1787592, Length=318, Percent_Identity=55.0314465408805, Blast_Score=364, Evalue=1e-102,
Organism=Escherichia coli, GI1786877, Length=318, Percent_Identity=55.0314465408805, Blast_Score=364, Evalue=1e-102,
Organism=Escherichia coli, GI1786764, Length=318, Percent_Identity=55.0314465408805, Blast_Score=364, Evalue=1e-102,
Organism=Escherichia coli, GI1786453, Length=318, Percent_Identity=55.0314465408805, Blast_Score=364, Evalue=1e-102,
Organism=Escherichia coli, GI1787634, Length=316, Percent_Identity=55.0632911392405, Blast_Score=362, Evalue=1e-101,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002559
- InterPro:   IPR008490 [H]

Pfam domain/function: PF05598 DUF772; PF01609 Transposase_11 [H]

EC number: NA

Molecular weight: Translated: 37054; Mature: 37054

Theoretical pI: Translated: 10.37; Mature: 10.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKQMTFADAEYAGKRKRTRKELFLIEMDQVVPWKGLIALIEPYYPKGEGGRPAYPLMAM
CCCCCCCCCHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHH
LRVHLMQNWFGYSDPAMEEALYETTILRQFSGLSLERIPDETTILNFRRLLEKHELATGI
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHHH
LGVINGYLGDRGLSLRQGTIVDATLIHAPSSTKNKDGKRDPEMHQTKKGNQYYFGAKAHI
HHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHCCCCEEEECCEEEC
GADDESGLVHSVVVTAANVADVTQVAKLLHGEENVVCADAGYTGVEKREEHTGRKVIWQI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHH
AARRSTYKKHGKRNVLYKAIRKIEKAKAQVRAKVEHPFRVIKRQFGYEKVRFRGLAKNTA
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
QMVTLFALSNLWMARRHLLASAGEVRV
HHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MMKQMTFADAEYAGKRKRTRKELFLIEMDQVVPWKGLIALIEPYYPKGEGGRPAYPLMAM
CCCCCCCCCHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHH
LRVHLMQNWFGYSDPAMEEALYETTILRQFSGLSLERIPDETTILNFRRLLEKHELATGI
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHHH
LGVINGYLGDRGLSLRQGTIVDATLIHAPSSTKNKDGKRDPEMHQTKKGNQYYFGAKAHI
HHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHCCCCEEEECCEEEC
GADDESGLVHSVVVTAANVADVTQVAKLLHGEENVVCADAGYTGVEKREEHTGRKVIWQI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHH
AARRSTYKKHGKRNVLYKAIRKIEKAKAQVRAKVEHPFRVIKRQFGYEKVRFRGLAKNTA
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
QMVTLFALSNLWMARRHLLASAGEVRV
HHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9097039; 9097040; 9278503 [H]