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The map label for this gene is nitA [H]

Identifier: 66043278

GI number: 66043278

Start: 8233

End: 9243

Strand: Reverse

Name: nitA [H]

Synonym: Psyr_0007

Alternate gene names: 66043278

Gene position: 9243-8233 (Counterclockwise)

Preceding gene: 66043280

Following gene: 66043277

Centisome position: 0.15

GC content: 58.75

Gene sequence:

>1011_bases
ATGAAAGAGCCTTTGAAAGTCGCCTGTGTGCAGGCTGCGCCCGTGTTCCTGGATCTGGACGCCACCGTAGACAAGACCAT
AACCCTGATGGAGCAGGCGGCAGCCGCCGGAGCCGGTCTTATTGCATTCCCGGAAACGTGGATTCCGGGCTATCCGTGGT
TTTTGTGGCTCGACGCACCGGCCTGGAACATGCCGTTGGTGCAGCGTTATCACCAGCAGTCTCTGGTGCTGGACAGTGTT
CAGGCCCGACGTATCAGCGATGCGGCTCGCCATCTCGGTCTCTATGTGGTGCTCGGTTACAGCGAGCGCAACAAAGCGTC
GCTGTATATCGGTCAGTGGATCATCGACGATCACGGTGAAACGGTTGGCGTGCGTCGCAAGCTCAAGGCTACTCATGTGG
AACGTACGATGTTTGGCGAAGGCGACGGGGCATCATTGCGTACCTTTGAAACCCCTGTCGGCGTTCTCGGTGCGCTTTGC
TGCTGGGAGCACCTGCAACCGCTTTCCAAGTACGCCATGTACGCACAGAACGAACAGATCCACGTAGCGGCCTGGCCAAG
CTTCAGCCTGTACCGCAACGCCACGAGTGCCCTGGGCCCCGAGGTCAATACTGCGGCTTCGCGGGTTTATGCCGCAGAAG
GGCAATGCTTCGTCCTCGCACCCTGTGCAATCGTTTCCCCTGAAATGATCGAAATGCTTTGCGACAGTGATGCCAAACGC
AGCCTGCTGCAGGCCGGTGGCGGGCACGCACGGATTTTCGGTCCGGACGGAAGTGATCTGGCGACCCCATTGGGCGAACA
TGAGGAAGGTCTGCTCTACGCAACACTTGATCCAGCCGCATTGACCTTGGCCAAAGTAGCAGCCGACCCGGCTGGACATT
ACTCACGTCCCGACGTCACTCGTCTGATGTTCAATCCCAATCCCACTCCATGTGTAGTCGATCTGCCAGACCTGCCGATC
AGCAGCGAGTCCATCGAGCTGCTTCGGCCTGACATCGCGCTGGAGGTGTGA

Upstream 100 bases:

>100_bases
CCTATCCCTTCCAGTCAAAAAGCCGTTCCTTTTCGTCCTAATACCGTCGTCAGGAAAGCTCATAGCCTGTGGCCATCCAA
CAGCCATTCGAGTTTTGACG

Downstream 100 bases:

>100_bases
CATGCACTCAGCGATTCCTGAACATCTGCGCAAGCCACGTGAGCTGAACAGCAATCTGCCCGAGGGCTATCAACCACCTT
TTCCTGCATGGTCCGCGCGT

Product: aliphatic nitrilase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MKEPLKVACVQAAPVFLDLDATVDKTITLMEQAAAAGAGLIAFPETWIPGYPWFLWLDAPAWNMPLVQRYHQQSLVLDSV
QARRISDAARHLGLYVVLGYSERNKASLYIGQWIIDDHGETVGVRRKLKATHVERTMFGEGDGASLRTFETPVGVLGALC
CWEHLQPLSKYAMYAQNEQIHVAAWPSFSLYRNATSALGPEVNTAASRVYAAEGQCFVLAPCAIVSPEMIEMLCDSDAKR
SLLQAGGGHARIFGPDGSDLATPLGEHEEGLLYATLDPAALTLAKVAADPAGHYSRPDVTRLMFNPNPTPCVVDLPDLPI
SSESIELLRPDIALEV

Sequences:

>Translated_336_residues
MKEPLKVACVQAAPVFLDLDATVDKTITLMEQAAAAGAGLIAFPETWIPGYPWFLWLDAPAWNMPLVQRYHQQSLVLDSV
QARRISDAARHLGLYVVLGYSERNKASLYIGQWIIDDHGETVGVRRKLKATHVERTMFGEGDGASLRTFETPVGVLGALC
CWEHLQPLSKYAMYAQNEQIHVAAWPSFSLYRNATSALGPEVNTAASRVYAAEGQCFVLAPCAIVSPEMIEMLCDSDAKR
SLLQAGGGHARIFGPDGSDLATPLGEHEEGLLYATLDPAALTLAKVAADPAGHYSRPDVTRLMFNPNPTPCVVDLPDLPI
SSESIELLRPDIALEV
>Mature_336_residues
MKEPLKVACVQAAPVFLDLDATVDKTITLMEQAAAAGAGLIAFPETWIPGYPWFLWLDAPAWNMPLVQRYHQQSLVLDSV
QARRISDAARHLGLYVVLGYSERNKASLYIGQWIIDDHGETVGVRRKLKATHVERTMFGEGDGASLRTFETPVGVLGALC
CWEHLQPLSKYAMYAQNEQIHVAAWPSFSLYRNATSALGPEVNTAASRVYAAEGQCFVLAPCAIVSPEMIEMLCDSDAKR
SLLQAGGGHARIFGPDGSDLATPLGEHEEGLLYATLDPAALTLAKVAADPAGHYSRPDVTRLMFNPNPTPCVVDLPDLPI
SSESIELLRPDIALEV

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI17557111, Length=316, Percent_Identity=31.0126582278481, Blast_Score=142, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6322027, Length=195, Percent_Identity=28.2051282051282, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000132
- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.5.7 [H]

Molecular weight: Translated: 36553; Mature: 36553

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00920 NITRIL_CHT_1 ; PS00921 NITRIL_CHT_2 ; PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEPLKVACVQAAPVFLDLDATVDKTITLMEQAAAAGAGLIAFPETWIPGYPWFLWLDAP
CCCCCEEEEEEECCEEEEECCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEECCC
AWNMPLVQRYHQQSLVLDSVQARRISDAARHLGLYVVLGYSERNKASLYIGQWIIDDHGE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCEEEEEEEEEECCCCC
TVGVRRKLKATHVERTMFGEGDGASLRTFETPVGVLGALCCWEHLQPLSKYAMYAQNEQI
EEHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEE
HVAAWPSFSLYRNATSALGPEVNTAASRVYAAEGQCFVLAPCAIVSPEMIEMLCDSDAKR
EEEECCCHHHHHHHHHHCCCCCCHHHHEEEECCCCEEEEECHHHCCHHHHHHHHCCHHHH
SLLQAGGGHARIFGPDGSDLATPLGEHEEGLLYATLDPAALTLAKVAADPAGHYSRPDVT
HHHHCCCCEEEEECCCCCHHCCCCCCCCCCEEEEEECHHHHHHHHHHCCCCCCCCCCCEE
RLMFNPNPTPCVVDLPDLPISSESIELLRPDIALEV
EEEECCCCCCEEEECCCCCCCCCCHHHCCCCEEECC
>Mature Secondary Structure
MKEPLKVACVQAAPVFLDLDATVDKTITLMEQAAAAGAGLIAFPETWIPGYPWFLWLDAP
CCCCCEEEEEEECCEEEEECCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEECCC
AWNMPLVQRYHQQSLVLDSVQARRISDAARHLGLYVVLGYSERNKASLYIGQWIIDDHGE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCEEEEEEEEEECCCCC
TVGVRRKLKATHVERTMFGEGDGASLRTFETPVGVLGALCCWEHLQPLSKYAMYAQNEQI
EEHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEE
HVAAWPSFSLYRNATSALGPEVNTAASRVYAAEGQCFVLAPCAIVSPEMIEMLCDSDAKR
EEEECCCHHHHHHHHHHCCCCCCHHHHEEEECCCCEEEEECHHHCCHHHHHHHHCCHHHH
SLLQAGGGHARIFGPDGSDLATPLGEHEEGLLYATLDPAALTLAKVAADPAGHYSRPDVT
HHHHCCCCEEEEECCCCCHHCCCCCCCCCCEEEEEECHHHHHHHHHHCCCCCCCCCCCEE
RLMFNPNPTPCVVDLPDLPISSESIELLRPDIALEV
EEEECCCCCCEEEECCCCCCCCCCHHHCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1400390 [H]