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The map label for this gene is proP [H]

Identifier: 62391920

GI number: 62391920

Start: 3256778

End: 3257590

Strand: Reverse

Name: proP [H]

Synonym: cg3403

Alternate gene names: 62391920

Gene position: 3257590-3256778 (Counterclockwise)

Preceding gene: 62391921

Following gene: 62391917

Centisome position: 99.23

GC content: 55.84

Gene sequence:

>813_bases
GTGTGTGCTAGCCTTTTCGCAATCGTTCAGCCCGCCCCGACGTCAATGTTGGGAGTGGGCTGGCGCATTCCATTCCTGAT
GGCCGTGCCACTAGGGCTTATCGGCTGGTGGATCCGCACCGGTGCCCAGGAAAATGTACGCCCCGCATCCGAACGCCCCG
AAGCTCCTATTAAGCAGGCATTGCGTACTGAGTGGAAGATGATGTTGCGGGTAGGTGGCTTTATCTCTTGCACCGGTCTG
AGCTTCTACATTTTCACCACGTACATGACCACTTTCCTGCGCAGCACCGTCGGACTGGAGGGCACGTTAGTGCTGGCTGG
AAACATCATCGCTCTCAGCATGGCAGCAATTGTGGCCCCATTTGTTGGCCGCGCAATTGATAAATTCCCCCGCCGGAACA
TCATGGCTTTCGCTACCTTAAGCACAGTAATTATGGCGATCCCGGCCTACATCATTGCAGGTCAAGGTACTTTGACTGCT
TCTTTGATTGCGCAGGTAATGCTTGGAATCGGCGCGGTTACCGCTAACTGCGTTACCTCAGTAATGATGGCCGAGGTCTT
CCAAGAGGTCACCCGCGGTACTTCCGCCGGCATTACCTACAACGTCACTTACGCAATCTTCGGCGGCTCGGCTCCATTTA
TCTCCACCGCATTGGTCTCCTGGACCGGCAGCCCGCTGGCCCCTGCGGTATACATGATCATCATTGCGCTCTTCGCCTTC
ACCGCGTCCCGCTTCATTCCTGAAACCTCCCCAGTTTTTGTCACCGCAACCCCGGCCATTAAGGCACCAAAGGTGCTGGT
CAACCCGGGTTAA

Upstream 100 bases:

>100_bases
CAATTGAGCTGGTCAGCGCCTTGCAGTTTGTGCCGCGCTTAATTGCTTATCGACGTCCCCATCCCCCTCGCCAATGCTTC
GGCGAGGGGTTCTATTTATT

Downstream 100 bases:

>100_bases
ACCACGCTTTTCGACGAAAAAACCTGCCTCTTCACCAGGTATAAAATGGTGAAGAGGCAGGTTTTTAAATGTTCTTGGGT
GCTTTAGGAGGCAACGATCT

Product: major facilitator superfamily permease

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MCASLFAIVQPAPTSMLGVGWRIPFLMAVPLGLIGWWIRTGAQENVRPASERPEAPIKQALRTEWKMMLRVGGFISCTGL
SFYIFTTYMTTFLRSTVGLEGTLVLAGNIIALSMAAIVAPFVGRAIDKFPRRNIMAFATLSTVIMAIPAYIIAGQGTLTA
SLIAQVMLGIGAVTANCVTSVMMAEVFQEVTRGTSAGITYNVTYAIFGGSAPFISTALVSWTGSPLAPAVYMIIIALFAF
TASRFIPETSPVFVTATPAIKAPKVLVNPG

Sequences:

>Translated_270_residues
MCASLFAIVQPAPTSMLGVGWRIPFLMAVPLGLIGWWIRTGAQENVRPASERPEAPIKQALRTEWKMMLRVGGFISCTGL
SFYIFTTYMTTFLRSTVGLEGTLVLAGNIIALSMAAIVAPFVGRAIDKFPRRNIMAFATLSTVIMAIPAYIIAGQGTLTA
SLIAQVMLGIGAVTANCVTSVMMAEVFQEVTRGTSAGITYNVTYAIFGGSAPFISTALVSWTGSPLAPAVYMIIIALFAF
TASRFIPETSPVFVTATPAIKAPKVLVNPG
>Mature_270_residues
MCASLFAIVQPAPTSMLGVGWRIPFLMAVPLGLIGWWIRTGAQENVRPASERPEAPIKQALRTEWKMMLRVGGFISCTGL
SFYIFTTYMTTFLRSTVGLEGTLVLAGNIIALSMAAIVAPFVGRAIDKFPRRNIMAFATLSTVIMAIPAYIIAGQGTLTA
SLIAQVMLGIGAVTANCVTSVMMAEVFQEVTRGTSAGITYNVTYAIFGGSAPFISTALVSWTGSPLAPAVYMIIIALFAF
TASRFIPETSPVFVTATPAIKAPKVLVNPG

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=279, Percent_Identity=25.089605734767, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788942, Length=235, Percent_Identity=28.936170212766, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788292, Length=230, Percent_Identity=25.6521739130435, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 28684; Mature: 28684

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCASLFAIVQPAPTSMLGVGWRIPFLMAVPLGLIGWWIRTGAQENVRPASERPEAPIKQA
CCCHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
LRTEWKMMLRVGGFISCTGLSFYIFTTYMTTFLRSTVGLEGTLVLAGNIIALSMAAIVAP
HHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHH
FVGRAIDKFPRRNIMAFATLSTVIMAIPAYIIAGQGTLTASLIAQVMLGIGAVTANCVTS
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHH
VMMAEVFQEVTRGTSAGITYNVTYAIFGGSAPFISTALVSWTGSPLAPAVYMIIIALFAF
HHHHHHHHHHHCCCCCCEEEEEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
TASRFIPETSPVFVTATPAIKAPKVLVNPG
HHHHCCCCCCCEEEEECCCCCCCEEEECCC
>Mature Secondary Structure
MCASLFAIVQPAPTSMLGVGWRIPFLMAVPLGLIGWWIRTGAQENVRPASERPEAPIKQA
CCCHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
LRTEWKMMLRVGGFISCTGLSFYIFTTYMTTFLRSTVGLEGTLVLAGNIIALSMAAIVAP
HHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHH
FVGRAIDKFPRRNIMAFATLSTVIMAIPAYIIAGQGTLTASLIAQVMLGIGAVTANCVTS
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHH
VMMAEVFQEVTRGTSAGITYNVTYAIFGGSAPFISTALVSWTGSPLAPAVYMIIIALFAF
HHHHHHHHHHHCCCCCCEEEEEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
TASRFIPETSPVFVTATPAIKAPKVLVNPG
HHHHCCCCCCCEEEEECCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]