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The map label for this gene is dnaB [H]

Identifier: 62391827

GI number: 62391827

Start: 3154641

End: 3156173

Strand: Reverse

Name: dnaB [H]

Synonym: cg3304

Alternate gene names: 62391827

Gene position: 3156173-3154641 (Counterclockwise)

Preceding gene: 62391828

Following gene: 62391826

Centisome position: 96.15

GC content: 56.29

Gene sequence:

>1533_bases
ATGGCTACAGATACACACGCCGCAAGTTTTGATGATGACTACGTACCTCCACAGGAGCCAAGCGATTCCTTCGCGGATGA
CGCACACGTAGATGTCCCTGCTCCGGCGTTTGAAGATTTCTCCCCGGCCCAGGCCTTTGGTCAGGGAACTAGGGGAGGAG
ACTCTCAGGGCTTCAAGAAGAGGGGTCGCAAGGACGAGTCCCGTGAATACCGAGACTTCCGCCAACCTCCCTATGACAAT
GACGCTGAGATGGGCGTGCTGGGCGCGATGCTGCTCAGTCCGACCACGGTCATCGACATTCTGGATATCCTCACCCCAGA
AGACTTCTACAGGCCGTCCCACCAGCTGATTTTCCAGGCGATCATTGACCTGTTCAGTGACAACCGTGATATTGACCCCG
TGATTGTCTCCGGTCGCCTCGATCGAACCAACGATCTGGACCGCGTCGGCGGTGGCGCGTACCTCCACGACCTCATCCAG
TCCGTTCCCACCGCAGCCAACGCGCGCTACTATGCGGAAATCGTTTCGGAAAAGGCAGTGCTTCGCAGGCTTGTCGACGC
CGGCACCCGCGTCGTCCAGCTCGGCTACGAGGGCGATGAAGGCGCCGAAATTGACGCGGTGATTGACCGCGCGCAGCAAG
AAGTCTTCGCCGTTTCCCAAAAGAATCAGAGCGAAGACTATGCAGTCCTAGCCGATATTCTGGATGAAACCATGGCTGAG
CTGGAAATGCTCAACGACGGCGGCATCGCCACCGGTATTCCAACCGGCTTCAAAGATCTCGATGACCTCACCAACGGTCT
GCGCGGTGGCCAGATGATCATCGTTGCAGCTCGTCCTGGTGTGGGTAAATCCACCATCGCCTTGGACTTCATGCGTTCGG
CATCCATCAAGAACAACATGGCGTCTGTCATTTTCTCCTTGGAAATGTCCAAGTCAGAGATCGTGATGCGTTTGCTCTCT
GCAGAAACAGAAATCCGCCTGGCTGATATGCGTGGTGGAAAGATGGATGAAACCGCATGGGAAAAGATGGTGCAGAAGTT
AGACAAGGTAGCCCAGGCACCTTTGTTCATCGATGACTCCGCGAACCTCACCATGATGGAAATCCGCTCCAAGGCCAGAA
AGCTGAAGCAGAAGCATGATCTGAAAATGATCGTGGTGGACTACCTCCAGCTGATGAGCTCCGGTAAACGCGTGGAATCC
CGTCAGCAGGAAGTCTCCGAGTTCTCCCGTCAGCTCAAGCTGCTGGCCAAAGAACTCGATGTGCCGTTGATCGCGATTTC
CCAGCTGAACCGTGGACCTGAATCCCGTACCGATAAGCGACCACAGCTTGCTGACCTTCGTGAATCCGGCTCGCTGGAGC
AGGACGCCGATATCGTTATGCTGCTATACCGCCCAGACTCCCAGGATAAGGACGACGAGCGCGCGGGCGAGGCCGACATC
ATTTTGGCTAAGCACCGTGGTGGCCCGATCGATACCGTCCAGGTGGCGCACCAGCTGCACTATTCACGTTTTGTGGACAT
GGCGCGCGGTTAA

Upstream 100 bases:

>100_bases
AGTTGGAAATCACAAAGCCTCGGGGTGGGGGCTTTCGTGCCTTCTGGAAAGGCCTATGCAGGGGCTAGAGTGAACTTAGA
ATCAGATTGGGGAAAAGAAA

Downstream 100 bases:

>100_bases
GAAAAGTTCATTTTTCTAAAGGCAGTGACTCCGATGTGGGGCACTGCCTTTAGTGATTCTAAGTCACTCAGCCCAGGCAA
TCCTATCTTTGGCAAGACTT

Product: replicative DNA helicase

Products: NA

Alternate protein names: Mbo dnaB intein [H]

Number of amino acids: Translated: 510; Mature: 509

Protein sequence:

>510_residues
MATDTHAASFDDDYVPPQEPSDSFADDAHVDVPAPAFEDFSPAQAFGQGTRGGDSQGFKKRGRKDESREYRDFRQPPYDN
DAEMGVLGAMLLSPTTVIDILDILTPEDFYRPSHQLIFQAIIDLFSDNRDIDPVIVSGRLDRTNDLDRVGGGAYLHDLIQ
SVPTAANARYYAEIVSEKAVLRRLVDAGTRVVQLGYEGDEGAEIDAVIDRAQQEVFAVSQKNQSEDYAVLADILDETMAE
LEMLNDGGIATGIPTGFKDLDDLTNGLRGGQMIIVAARPGVGKSTIALDFMRSASIKNNMASVIFSLEMSKSEIVMRLLS
AETEIRLADMRGGKMDETAWEKMVQKLDKVAQAPLFIDDSANLTMMEIRSKARKLKQKHDLKMIVVDYLQLMSSGKRVES
RQQEVSEFSRQLKLLAKELDVPLIAISQLNRGPESRTDKRPQLADLRESGSLEQDADIVMLLYRPDSQDKDDERAGEADI
ILAKHRGGPIDTVQVAHQLHYSRFVDMARG

Sequences:

>Translated_510_residues
MATDTHAASFDDDYVPPQEPSDSFADDAHVDVPAPAFEDFSPAQAFGQGTRGGDSQGFKKRGRKDESREYRDFRQPPYDN
DAEMGVLGAMLLSPTTVIDILDILTPEDFYRPSHQLIFQAIIDLFSDNRDIDPVIVSGRLDRTNDLDRVGGGAYLHDLIQ
SVPTAANARYYAEIVSEKAVLRRLVDAGTRVVQLGYEGDEGAEIDAVIDRAQQEVFAVSQKNQSEDYAVLADILDETMAE
LEMLNDGGIATGIPTGFKDLDDLTNGLRGGQMIIVAARPGVGKSTIALDFMRSASIKNNMASVIFSLEMSKSEIVMRLLS
AETEIRLADMRGGKMDETAWEKMVQKLDKVAQAPLFIDDSANLTMMEIRSKARKLKQKHDLKMIVVDYLQLMSSGKRVES
RQQEVSEFSRQLKLLAKELDVPLIAISQLNRGPESRTDKRPQLADLRESGSLEQDADIVMLLYRPDSQDKDDERAGEADI
ILAKHRGGPIDTVQVAHQLHYSRFVDMARG
>Mature_509_residues
ATDTHAASFDDDYVPPQEPSDSFADDAHVDVPAPAFEDFSPAQAFGQGTRGGDSQGFKKRGRKDESREYRDFRQPPYDND
AEMGVLGAMLLSPTTVIDILDILTPEDFYRPSHQLIFQAIIDLFSDNRDIDPVIVSGRLDRTNDLDRVGGGAYLHDLIQS
VPTAANARYYAEIVSEKAVLRRLVDAGTRVVQLGYEGDEGAEIDAVIDRAQQEVFAVSQKNQSEDYAVLADILDETMAEL
EMLNDGGIATGIPTGFKDLDDLTNGLRGGQMIIVAARPGVGKSTIALDFMRSASIKNNMASVIFSLEMSKSEIVMRLLSA
ETEIRLADMRGGKMDETAWEKMVQKLDKVAQAPLFIDDSANLTMMEIRSKARKLKQKHDLKMIVVDYLQLMSSGKRVESR
QQEVSEFSRQLKLLAKELDVPLIAISQLNRGPESRTDKRPQLADLRESGSLEQDADIVMLLYRPDSQDKDDERAGEADII
LAKHRGGPIDTVQVAHQLHYSRFVDMARG

Specific function: The intein is an endonuclease (Potential) [H]

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=444, Percent_Identity=38.2882882882883, Blast_Score=305, Evalue=4e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136
- InterPro:   IPR003586
- InterPro:   IPR003587
- InterPro:   IPR006142
- InterPro:   IPR004042
- InterPro:   IPR006141 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 56589; Mature: 56458

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATDTHAASFDDDYVPPQEPSDSFADDAHVDVPAPAFEDFSPAQAFGQGTRGGDSQGFKK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHH
RGRKDESREYRDFRQPPYDNDAEMGVLGAMLLSPTTVIDILDILTPEDFYRPSHQLIFQA
CCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHCCCHHHCCCHHHHHHHH
IIDLFSDNRDIDPVIVSGRLDRTNDLDRVGGGAYLHDLIQSVPTAANARYYAEIVSEKAV
HHHHHCCCCCCCCEEEECCCCCCCCHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LRRLVDAGTRVVQLGYEGDEGAEIDAVIDRAQQEVFAVSQKNQSEDYAVLADILDETMAE
HHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
LEMLNDGGIATGIPTGFKDLDDLTNGLRGGQMIIVAARPGVGKSTIALDFMRSASIKNNM
HHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHH
ASVIFSLEMSKSEIVMRLLSAETEIRLADMRGGKMDETAWEKMVQKLDKVAQAPLFIDDS
HHHHHHHHCCHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCEEEECC
ANLTMMEIRSKARKLKQKHDLKMIVVDYLQLMSSGKRVESRQQEVSEFSRQLKLLAKELD
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHC
VPLIAISQLNRGPESRTDKRPQLADLRESGSLEQDADIVMLLYRPDSQDKDDERAGEADI
CCEEEHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHCCCCEE
ILAKHRGGPIDTVQVAHQLHYSRFVDMARG
EEEECCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ATDTHAASFDDDYVPPQEPSDSFADDAHVDVPAPAFEDFSPAQAFGQGTRGGDSQGFKK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHH
RGRKDESREYRDFRQPPYDNDAEMGVLGAMLLSPTTVIDILDILTPEDFYRPSHQLIFQA
CCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHCCCHHHCCCHHHHHHHH
IIDLFSDNRDIDPVIVSGRLDRTNDLDRVGGGAYLHDLIQSVPTAANARYYAEIVSEKAV
HHHHHCCCCCCCCEEEECCCCCCCCHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LRRLVDAGTRVVQLGYEGDEGAEIDAVIDRAQQEVFAVSQKNQSEDYAVLADILDETMAE
HHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
LEMLNDGGIATGIPTGFKDLDDLTNGLRGGQMIIVAARPGVGKSTIALDFMRSASIKNNM
HHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHH
ASVIFSLEMSKSEIVMRLLSAETEIRLADMRGGKMDETAWEKMVQKLDKVAQAPLFIDDS
HHHHHHHHCCHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCEEEECC
ANLTMMEIRSKARKLKQKHDLKMIVVDYLQLMSSGKRVESRQQEVSEFSRQLKLLAKELD
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHC
VPLIAISQLNRGPESRTDKRPQLADLRESGSLEQDADIVMLLYRPDSQDKDDERAGEADI
CCEEEHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHCCCCEE
ILAKHRGGPIDTVQVAHQLHYSRFVDMARG
EEEECCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]