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The map label for this gene is bmr3 [H]

Identifier: 62391771

GI number: 62391771

Start: 3107082

End: 3108548

Strand: Reverse

Name: bmr3 [H]

Synonym: cg3245

Alternate gene names: 62391771

Gene position: 3108548-3107082 (Counterclockwise)

Preceding gene: 62391773

Following gene: 62391769

Centisome position: 94.69

GC content: 54.33

Gene sequence:

>1467_bases
ATGTCATCGAGTCCTCCCGAATCAGCCACACCACAGATCAAATACGGCCTGCTGGTGGTCACTCTCGCCTCAGCTGGTAT
CACTGTTTCCCTAGCGCAGACCCTGGTTATTCCGATCATTGGTCGGTTGCCCGAGATCTTCAACACCACGGCTGCTAATG
CCTCTTGGATCATTACTGTGACGCTGTTGGTGGGCGCAGTGGCGACTCCTGTGATGGGCAGGCTTGCAGATATGTACGGC
AAGAAAAAGATGATGCTCATCTCACTTGTCCCGTTCATTCTTGGATCAGTGATCTGCGCTGTGTCGGTGGATTTGATTCC
GATGATCATCGGCCGTGGTTTTCAGGGGCTTGGCTCTGGCCTGATTCCTCTTGGCATTTCTCTCATGCATGATTTGTTGC
CCCGGGAGAAAGCAGGGTCTGCCATTGCTTTGATGAGTTCTTCCATGGGCATTGGCGGTGCACTCGGTCTACCGCTGGCT
GCTGCTATTGCCCAGTTTGCGTCCTGGCGGGTGCTGTTCTGGTTCACCGCTCTGGTAGCGCTTACAGTTGGCGCGGTCAT
TTGGAAGGCGATTCCTGCTAGACCCAGGATCGTGAGGAGTGGCGGCTTTGATTATTTCGGTGCTCTCGGCCTTGCAATGG
GACTTATCGCATTGTTGCTCGCGGTGTCCAAGGGATCAGAATGGGGCTGGAGAAGTGCCCTGACCATTGGGTTATTCGTG
GCAGCGCTGGTGATTTTGGTGGGTTGGGGCTGGTTCGAAACCCGCCAGAAATCCCCTTTGATTGATCTGCGCACCACTAT
TCGGGCGACCGTGTTGATGACAAATATTGCGTCCATCCTCATCGGTTTCACCATGTATGGAATGAATCTGATCCTGCCTC
AGGTCATGCAGCTGCCTGTAATTCTGGGCTACGGTCTAGGCCAGAGCATGCTTCAGATGGGCATCTGGCTGATCCCGATG
GGTCTAGGCATGATGTTGATTTCGAATGCAGGTGCAGCCATTAGCGCTGCTCATGGTCCTCGTGTGACGCTGACAATTGC
GGGTGTTGTGATCGCAGTCGGTTATGCACTTACTGCCACAGTGTTGTTCACTATCGGCAACCGCACACCGGGAGGAGATG
CAGACAACGCACTTATTTTGACCACCCTGGTGCTGTTCTCAGTGTGTAGTCTCGTGGTCGGTATCGGCATTGGCCTGGCA
TTTGGTTCCATGCCTGCCTTGATCATGGGTGCCGTACCAGCCACGGAGAAAGCCGCAGCGAATGGTTTCAACTCTCTTAT
GCGTTCACTGGGCACCACCGGCTCATCAGCTGTCATCGGTGCAGTGTTGGCCGGAATGATGAGTGGCGGAGTACCCACCT
TAGGGGGATTCATGACCACTCTGATCATCGGATGCTGCGCCGCGCTTGTGGCTGCGGTCATCTCCTATTTCATCCCCACC
ACAACCACTGTGGTGGAAGCAAAATAA

Upstream 100 bases:

>100_bases
GATGGTGGATTCGTATTCCAAGGCCGCGATGGGGTCGGTGCTCATGGCTCTCCAGTTTGCCAGCTATTGCATCTTGCACA
AAATGTGTACCATACACATA

Downstream 100 bases:

>100_bases
TCCCGGCAGCGACTCGACCAGCAGCTACTTGTCCAGTAGTCGATCCTGAATCTGATCTGACACCTGGTTCAGATCACGAC
TGATCGACTGGTGCGCACGG

Product: major facilitator superfamily permease

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter 3 [H]

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MSSSPPESATPQIKYGLLVVTLASAGITVSLAQTLVIPIIGRLPEIFNTTAANASWIITVTLLVGAVATPVMGRLADMYG
KKKMMLISLVPFILGSVICAVSVDLIPMIIGRGFQGLGSGLIPLGISLMHDLLPREKAGSAIALMSSSMGIGGALGLPLA
AAIAQFASWRVLFWFTALVALTVGAVIWKAIPARPRIVRSGGFDYFGALGLAMGLIALLLAVSKGSEWGWRSALTIGLFV
AALVILVGWGWFETRQKSPLIDLRTTIRATVLMTNIASILIGFTMYGMNLILPQVMQLPVILGYGLGQSMLQMGIWLIPM
GLGMMLISNAGAAISAAHGPRVTLTIAGVVIAVGYALTATVLFTIGNRTPGGDADNALILTTLVLFSVCSLVVGIGIGLA
FGSMPALIMGAVPATEKAAANGFNSLMRSLGTTGSSAVIGAVLAGMMSGGVPTLGGFMTTLIIGCCAALVAAVISYFIPT
TTTVVEAK

Sequences:

>Translated_488_residues
MSSSPPESATPQIKYGLLVVTLASAGITVSLAQTLVIPIIGRLPEIFNTTAANASWIITVTLLVGAVATPVMGRLADMYG
KKKMMLISLVPFILGSVICAVSVDLIPMIIGRGFQGLGSGLIPLGISLMHDLLPREKAGSAIALMSSSMGIGGALGLPLA
AAIAQFASWRVLFWFTALVALTVGAVIWKAIPARPRIVRSGGFDYFGALGLAMGLIALLLAVSKGSEWGWRSALTIGLFV
AALVILVGWGWFETRQKSPLIDLRTTIRATVLMTNIASILIGFTMYGMNLILPQVMQLPVILGYGLGQSMLQMGIWLIPM
GLGMMLISNAGAAISAAHGPRVTLTIAGVVIAVGYALTATVLFTIGNRTPGGDADNALILTTLVLFSVCSLVVGIGIGLA
FGSMPALIMGAVPATEKAAANGFNSLMRSLGTTGSSAVIGAVLAGMMSGGVPTLGGFMTTLIIGCCAALVAAVISYFIPT
TTTVVEAK
>Mature_487_residues
SSSPPESATPQIKYGLLVVTLASAGITVSLAQTLVIPIIGRLPEIFNTTAANASWIITVTLLVGAVATPVMGRLADMYGK
KKMMLISLVPFILGSVICAVSVDLIPMIIGRGFQGLGSGLIPLGISLMHDLLPREKAGSAIALMSSSMGIGGALGLPLAA
AIAQFASWRVLFWFTALVALTVGAVIWKAIPARPRIVRSGGFDYFGALGLAMGLIALLLAVSKGSEWGWRSALTIGLFVA
ALVILVGWGWFETRQKSPLIDLRTTIRATVLMTNIASILIGFTMYGMNLILPQVMQLPVILGYGLGQSMLQMGIWLIPMG
LGMMLISNAGAAISAAHGPRVTLTIAGVVIAVGYALTATVLFTIGNRTPGGDADNALILTTLVLFSVCSLVVGIGIGLAF
GSMPALIMGAVPATEKAAANGFNSLMRSLGTTGSSAVIGAVLAGMMSGGVPTLGGFMTTLIIGCCAALVAAVISYFIPTT
TTVVEAK

Specific function: Confers resistance to puromycin, tosufloxacin and norfloxacin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1788710, Length=312, Percent_Identity=27.5641025641026, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI87081983, Length=419, Percent_Identity=24.3436754176611, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1789042, Length=395, Percent_Identity=23.2911392405063, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1788392, Length=299, Percent_Identity=28.7625418060201, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324954, Length=224, Percent_Identity=25, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50223; Mature: 50092

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSPPESATPQIKYGLLVVTLASAGITVSLAQTLVIPIIGRLPEIFNTTAANASWIITV
CCCCCCCCCCCCHHHHHHHEEHHHCCCHHHHHHHHHHHHHHHCHHHHCCCCCCCHHHHHH
TLLVGAVATPVMGRLADMYGKKKMMLISLVPFILGSVICAVSVDLIPMIIGRGFQGLGSG
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCC
LIPLGISLMHDLLPREKAGSAIALMSSSMGIGGALGLPLAAAIAQFASWRVLFWFTALVA
HHHHHHHHHHHHCCCHHCCCEEEEHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTVGAVIWKAIPARPRIVRSGGFDYFGALGLAMGLIALLLAVSKGSEWGWRSALTIGLFV
HHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
AALVILVGWGWFETRQKSPLIDLRTTIRATVLMTNIASILIGFTMYGMNLILPQVMQLPV
HHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILGYGLGQSMLQMGIWLIPMGLGMMLISNAGAAISAAHGPRVTLTIAGVVIAVGYALTAT
HHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEHHHHHHHHHHHHHHHH
VLFTIGNRTPGGDADNALILTTLVLFSVCSLVVGIGIGLAFGSMPALIMGAVPATEKAAA
HHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCHHHHH
NGFNSLMRSLGTTGSSAVIGAVLAGMMSGGVPTLGGFMTTLIIGCCAALVAAVISYFIPT
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TTTVVEAK
CCEEEECC
>Mature Secondary Structure 
SSSPPESATPQIKYGLLVVTLASAGITVSLAQTLVIPIIGRLPEIFNTTAANASWIITV
CCCCCCCCCCCHHHHHHHEEHHHCCCHHHHHHHHHHHHHHHCHHHHCCCCCCCHHHHHH
TLLVGAVATPVMGRLADMYGKKKMMLISLVPFILGSVICAVSVDLIPMIIGRGFQGLGSG
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCC
LIPLGISLMHDLLPREKAGSAIALMSSSMGIGGALGLPLAAAIAQFASWRVLFWFTALVA
HHHHHHHHHHHHCCCHHCCCEEEEHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTVGAVIWKAIPARPRIVRSGGFDYFGALGLAMGLIALLLAVSKGSEWGWRSALTIGLFV
HHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
AALVILVGWGWFETRQKSPLIDLRTTIRATVLMTNIASILIGFTMYGMNLILPQVMQLPV
HHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILGYGLGQSMLQMGIWLIPMGLGMMLISNAGAAISAAHGPRVTLTIAGVVIAVGYALTAT
HHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEHHHHHHHHHHHHHHHH
VLFTIGNRTPGGDADNALILTTLVLFSVCSLVVGIGIGLAFGSMPALIMGAVPATEKAAA
HHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCHHHHH
NGFNSLMRSLGTTGSSAVIGAVLAGMMSGGVPTLGGFMTTLIIGCCAALVAAVISYFIPT
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TTTVVEAK
CCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9023234; 9384377 [H]