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The map label for this gene is grpE [H]

Identifier: 62391634

GI number: 62391634

Start: 2957190

End: 2957846

Strand: Reverse

Name: grpE [H]

Synonym: cg3099

Alternate gene names: 62391634

Gene position: 2957846-2957190 (Counterclockwise)

Preceding gene: 62391635

Following gene: 62391633

Centisome position: 90.1

GC content: 55.25

Gene sequence:

>657_bases
ATGACTACCCCTAACGGAATGCCCGACAATCCTGGGGATCCTGAAAATACCGATCCAGAGGCAACCTCTGCTGATCGTGC
TGAGCAGGCAGCTGAAGAAGCAGCTGCCCGCCAAGCGGAGGAATCTCCATTTGGACAGGCCTCAGAGGAAGAAATTTCTC
CAGAGCTCGAAGCAGAGATCAATGATCTTCTATCAGATGTTGATCCAGATTTGGATGGCGATGGTGAAGTGTCCGCTGTA
GAAACACAGCTTGCCGAACGCACTGAGGATCTGCAGCGAGTCACCGCTGAGTACGCCAACTACCGTCGACGTACCGAGCG
TGAACGCCAGGGCATCATCGACACCGCACGCGCAGGTGTTGTTACCCAACTTCTGCCGTTGCTCGACGATCTTGACCTGG
CTGAACAGCACGGTGACCTTAACGAAGGTCCGCTGAAGTCACTGTCTGACAAGCTGATCAACATCCTGGGTGGATTGAAG
GTGGAATCCTTCGGCGAGATCGGCGAAGCATTCGATCCAGAGATCCACGAAGCAGTACAGGATCTCTCACAGGGTGATGT
CAAGGTTCTGGGAACGGTACTCCGCAAGGGATACCGCCTCGGCGACCGCGTCATCCGCACCGCAATGGTCCTCATTGGGG
ATCCAGAGGAGAGCTAG

Upstream 100 bases:

>100_bases
CCAGGCTGACGCAGGTGCAGAAGGCGCTGCAGATGACAATGTTGTTGACGCTGAAGTTGTCGAAGACGACGCAGCTGACA
ATGGTGAGGACAAGAAGTAA

Downstream 100 bases:

>100_bases
AGAGACTAAGTCTCTTAGTGGTTTCCGCCGTTGTCCACGGTCCACAGATGGAACTCCTTGAACAACACCGGCGGATATCC
TTGAAGTAATAAATACTCAA

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MTTPNGMPDNPGDPENTDPEATSADRAEQAAEEAAARQAEESPFGQASEEEISPELEAEINDLLSDVDPDLDGDGEVSAV
ETQLAERTEDLQRVTAEYANYRRRTERERQGIIDTARAGVVTQLLPLLDDLDLAEQHGDLNEGPLKSLSDKLINILGGLK
VESFGEIGEAFDPEIHEAVQDLSQGDVKVLGTVLRKGYRLGDRVIRTAMVLIGDPEES

Sequences:

>Translated_218_residues
MTTPNGMPDNPGDPENTDPEATSADRAEQAAEEAAARQAEESPFGQASEEEISPELEAEINDLLSDVDPDLDGDGEVSAV
ETQLAERTEDLQRVTAEYANYRRRTERERQGIIDTARAGVVTQLLPLLDDLDLAEQHGDLNEGPLKSLSDKLINILGGLK
VESFGEIGEAFDPEIHEAVQDLSQGDVKVLGTVLRKGYRLGDRVIRTAMVLIGDPEES
>Mature_217_residues
TTPNGMPDNPGDPENTDPEATSADRAEQAAEEAAARQAEESPFGQASEEEISPELEAEINDLLSDVDPDLDGDGEVSAVE
TQLAERTEDLQRVTAEYANYRRRTERERQGIIDTARAGVVTQLLPLLDDLDLAEQHGDLNEGPLKSLSDKLINILGGLKV
ESFGEIGEAFDPEIHEAVQDLSQGDVKVLGTVLRKGYRLGDRVIRTAMVLIGDPEES

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Escherichia coli, GI1788967, Length=198, Percent_Identity=31.8181818181818, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 23680; Mature: 23549

Theoretical pI: Translated: 3.87; Mature: 3.87

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPNGMPDNPGDPENTDPEATSADRAEQAAEEAAARQAEESPFGQASEEEISPELEAEI
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHHH
NDLLSDVDPDLDGDGEVSAVETQLAERTEDLQRVTAEYANYRRRTERERQGIIDTARAGV
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTQLLPLLDDLDLAEQHGDLNEGPLKSLSDKLINILGGLKVESFGEIGEAFDPEIHEAVQ
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHH
DLSQGDVKVLGTVLRKGYRLGDRVIRTAMVLIGDPEES
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TTPNGMPDNPGDPENTDPEATSADRAEQAAEEAAARQAEESPFGQASEEEISPELEAEI
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHHH
NDLLSDVDPDLDGDGEVSAVETQLAERTEDLQRVTAEYANYRRRTERERQGIIDTARAGV
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTQLLPLLDDLDLAEQHGDLNEGPLKSLSDKLINILGGLKVESFGEIGEAFDPEIHEAVQ
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHH
DLSQGDVKVLGTVLRKGYRLGDRVIRTAMVLIGDPEES
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA