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The map label for this gene is speE [H]

Identifier: 62391538

GI number: 62391538

Start: 2847210

End: 2848751

Strand: Reverse

Name: speE [H]

Synonym: cg2990

Alternate gene names: 62391538

Gene position: 2848751-2847210 (Counterclockwise)

Preceding gene: 62391539

Following gene: 62391536

Centisome position: 86.78

GC content: 55.51

Gene sequence:

>1542_bases
ATGTCTGATTTAGGACCCATCTGGCGCTGGCTGTTATTAGTTTCCGTCTCCATTTGTGCGGCATCGGGGCTGGTCTATGA
GCTAGCCCTGGTATCGCTTTCCACCAGCTTGAACGGTGGCGGAATTGTAGAAACCTCCCTCATCGTCGCAGGTTATGTAG
CTGCCCTTGGACTTGGTGCACTGCTGGTCAAGCCGTTTCTCAACTGGCCTGCGCAAACCTTCCTCGGTGTGGAAACCCTC
CTTGGACTTATTGGTGGTTGTTCCGCGCTGGTGCTGTATTTCACCTTCGCGACCATCGGCCAATCCCTGTGGATTCTGGT
GATTGCCACCGCTGCAATTGGCATCCTGGTCGGCGCTGAACTTCCACTGCTGATGACCATGATCCAGCAAGGCCGCCTCG
CCGACGCCAAAACCACAGGATCTCTGGTTGCCACCTTGAATGCTGCTGATTACCTTGGCGCACTTTTAGGTGGCCTGGCC
TGGCCTTTTGTGTTGCTGCCGTGGCTTGGCATGATGCGCGGTGCCGCAGCAGCCGGAATGATCAACCTCGTTGCAGCACT
ATTCGTGGGCTGTGTGCTGCTGCGACATTTGCTTCCGCGCACCCACTTCTTCGTATCCGTGGTGGCGCTTCTTCTCGCGA
TCGCAGCGCTAGCCACCGTGTTGGTGAAATCCGACGGGATCGTTGCCACCGCCCGCGCACAGCTCTACCGCGACCCCGTG
ATCTATTCACACCAATCTGACTACCAAGACATCGTAGTGACAGAACGAGGCAAAGACCGACGCCTCTACCTCAATGGCGG
TTTGCAGTATTCCACTCGTGACCAGCATAGATATACAGAATCACTGGTGTATCCAAGCCTTAATCCAGAGGCAGAATCGG
TGTTAATCATCGGCGGTGGCGATGGCCTCGCAGCACGGGAACTCCTCCGATTCCCATCAATGCAGATCACCCAAGTTGAA
TTAGACCCAGAAGTCATCGAAGTAGCCAACACAGTGCTGCGCTCTGACAATGGGGGAGCGATGGAAGATCCCCGCGTCTC
CATCATCGTTGACGACGCTTTCACCTGGCTGCGCTCCGGCGGAAATAATGGCGAAACTTACGATTCCATCATCATCGATC
TTCCCGACCCAAACAACGACACCATGGCCAGGCTGTATTCAGAAGAGTTCTACACCTTGGCCCGAGCACGACTGAACGAA
CAAGGCCGCATGGTGGTGCAATCCTCCAGCGCCTACACCACTCCAGATGTGTTCTGGCGAGTTGGAGCAACCTTGAAATC
GGCGGGCTGTGAACAAGTCATCCCATATCACGTGCATGTTCCCACATTTGGCGACTGGGGCTTCCAACTGTGTGGCCCTG
CCGACATGGAATTAGAGCTTCGGGAAGACACCCCGCCACTGACTTTCCTTAATGATGAAGTTCTGGTGGCTGCTGGGGTG
TTTGGGTTGGATAATCAGCCTCGTGAATTGGAACCTTCCACGCTGGATCATCCCCGCGTGGTGGAGGATCTGCGCAAGGG
ATACCGCGAATCAGGCGACTAG

Upstream 100 bases:

>100_bases
TTCCGGGACGTTTCCGTGATCTCGTGGAAGATCCCAAACTCCGTTCCGGTGCCGTCGTGGCCGCCGTCATCTTGATCGTG
GTGGGAACCGTAAACGCTGC

Downstream 100 bases:

>100_bases
CTGCAACGATGCGCTGTGTGGCGATGTAAGCGATCGGTATCTTCGAATGAGTATTTGTATCGGGATGGGTGTGAAAGTCC
CTTAAATCGCATTCTGTGAT

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 513; Mature: 512

Protein sequence:

>513_residues
MSDLGPIWRWLLLVSVSICAASGLVYELALVSLSTSLNGGGIVETSLIVAGYVAALGLGALLVKPFLNWPAQTFLGVETL
LGLIGGCSALVLYFTFATIGQSLWILVIATAAIGILVGAELPLLMTMIQQGRLADAKTTGSLVATLNAADYLGALLGGLA
WPFVLLPWLGMMRGAAAAGMINLVAALFVGCVLLRHLLPRTHFFVSVVALLLAIAALATVLVKSDGIVATARAQLYRDPV
IYSHQSDYQDIVVTERGKDRRLYLNGGLQYSTRDQHRYTESLVYPSLNPEAESVLIIGGGDGLAARELLRFPSMQITQVE
LDPEVIEVANTVLRSDNGGAMEDPRVSIIVDDAFTWLRSGGNNGETYDSIIIDLPDPNNDTMARLYSEEFYTLARARLNE
QGRMVVQSSSAYTTPDVFWRVGATLKSAGCEQVIPYHVHVPTFGDWGFQLCGPADMELELREDTPPLTFLNDEVLVAAGV
FGLDNQPRELEPSTLDHPRVVEDLRKGYRESGD

Sequences:

>Translated_513_residues
MSDLGPIWRWLLLVSVSICAASGLVYELALVSLSTSLNGGGIVETSLIVAGYVAALGLGALLVKPFLNWPAQTFLGVETL
LGLIGGCSALVLYFTFATIGQSLWILVIATAAIGILVGAELPLLMTMIQQGRLADAKTTGSLVATLNAADYLGALLGGLA
WPFVLLPWLGMMRGAAAAGMINLVAALFVGCVLLRHLLPRTHFFVSVVALLLAIAALATVLVKSDGIVATARAQLYRDPV
IYSHQSDYQDIVVTERGKDRRLYLNGGLQYSTRDQHRYTESLVYPSLNPEAESVLIIGGGDGLAARELLRFPSMQITQVE
LDPEVIEVANTVLRSDNGGAMEDPRVSIIVDDAFTWLRSGGNNGETYDSIIIDLPDPNNDTMARLYSEEFYTLARARLNE
QGRMVVQSSSAYTTPDVFWRVGATLKSAGCEQVIPYHVHVPTFGDWGFQLCGPADMELELREDTPPLTFLNDEVLVAAGV
FGLDNQPRELEPSTLDHPRVVEDLRKGYRESGD
>Mature_512_residues
SDLGPIWRWLLLVSVSICAASGLVYELALVSLSTSLNGGGIVETSLIVAGYVAALGLGALLVKPFLNWPAQTFLGVETLL
GLIGGCSALVLYFTFATIGQSLWILVIATAAIGILVGAELPLLMTMIQQGRLADAKTTGSLVATLNAADYLGALLGGLAW
PFVLLPWLGMMRGAAAAGMINLVAALFVGCVLLRHLLPRTHFFVSVVALLLAIAALATVLVKSDGIVATARAQLYRDPVI
YSHQSDYQDIVVTERGKDRRLYLNGGLQYSTRDQHRYTESLVYPSLNPEAESVLIIGGGDGLAARELLRFPSMQITQVEL
DPEVIEVANTVLRSDNGGAMEDPRVSIIVDDAFTWLRSGGNNGETYDSIIIDLPDPNNDTMARLYSEEFYTLARARLNEQ
GRMVVQSSSAYTTPDVFWRVGATLKSAGCEQVIPYHVHVPTFGDWGFQLCGPADMELELREDTPPLTFLNDEVLVAAGVF
GLDNQPRELEPSTLDHPRVVEDLRKGYRESGD

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG4262

COG function: function code R; Predicted spermidine synthase with an N-terminal membrane domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=239, Percent_Identity=28.8702928870293, Blast_Score=88, Evalue=2e-17,
Organism=Escherichia coli, GI1786312, Length=179, Percent_Identity=32.4022346368715, Blast_Score=94, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI25154106, Length=177, Percent_Identity=33.3333333333333, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI212645616, Length=172, Percent_Identity=33.7209302325581, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6325326, Length=246, Percent_Identity=28.4552845528455, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6323175, Length=182, Percent_Identity=31.8681318681319, Blast_Score=84, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24645443, Length=199, Percent_Identity=30.1507537688442, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 55471; Mature: 55339

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLGPIWRWLLLVSVSICAASGLVYELALVSLSTSLNGGGIVETSLIVAGYVAALGLGA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
LLVKPFLNWPAQTFLGVETLLGLIGGCSALVLYFTFATIGQSLWILVIATAAIGILVGAE
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LPLLMTMIQQGRLADAKTTGSLVATLNAADYLGALLGGLAWPFVLLPWLGMMRGAAAAGM
HHHHHHHHHCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
INLVAALFVGCVLLRHLLPRTHFFVSVVALLLAIAALATVLVKSDGIVATARAQLYRDPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHCCCE
IYSHQSDYQDIVVTERGKDRRLYLNGGLQYSTRDQHRYTESLVYPSLNPEAESVLIIGGG
EECCCCCCCEEEEEECCCCCEEEEECCEEECCCHHHHHHHHHCCCCCCCCCCEEEEEECC
DGLAARELLRFPSMQITQVELDPEVIEVANTVLRSDNGGAMEDPRVSIIVDDAFTWLRSG
CCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCEEEEEECHHHHHHHCC
GNNGETYDSIIIDLPDPNNDTMARLYSEEFYTLARARLNEQGRMVVQSSSAYTTPDVFWR
CCCCCEECEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHH
VGATLKSAGCEQVIPYHVHVPTFGDWGFQLCGPADMELELREDTPPLTFLNDEVLVAAGV
HCCHHHHCCCCCCCCEEEECCCCCCCCCEECCCCCCEEEECCCCCCEEEECCCEEEEEEE
FGLDNQPRELEPSTLDHPRVVEDLRKGYRESGD
ECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SDLGPIWRWLLLVSVSICAASGLVYELALVSLSTSLNGGGIVETSLIVAGYVAALGLGA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
LLVKPFLNWPAQTFLGVETLLGLIGGCSALVLYFTFATIGQSLWILVIATAAIGILVGAE
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LPLLMTMIQQGRLADAKTTGSLVATLNAADYLGALLGGLAWPFVLLPWLGMMRGAAAAGM
HHHHHHHHHCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
INLVAALFVGCVLLRHLLPRTHFFVSVVALLLAIAALATVLVKSDGIVATARAQLYRDPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHCCCE
IYSHQSDYQDIVVTERGKDRRLYLNGGLQYSTRDQHRYTESLVYPSLNPEAESVLIIGGG
EECCCCCCCEEEEEECCCCCEEEEECCEEECCCHHHHHHHHHCCCCCCCCCCEEEEEECC
DGLAARELLRFPSMQITQVELDPEVIEVANTVLRSDNGGAMEDPRVSIIVDDAFTWLRSG
CCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCEEEEEECHHHHHHHCC
GNNGETYDSIIIDLPDPNNDTMARLYSEEFYTLARARLNEQGRMVVQSSSAYTTPDVFWR
CCCCCEECEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHH
VGATLKSAGCEQVIPYHVHVPTFGDWGFQLCGPADMELELREDTPPLTFLNDEVLVAAGV
HCCHHHHCCCCCCCCEEEECCCCCCCCCEECCCCCCEEEECCCCCCEEEECCCEEEEEEE
FGLDNQPRELEPSTLDHPRVVEDLRKGYRESGD
ECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]