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The map label for this gene is purF [H]

Identifier: 62391423

GI number: 62391423

Start: 2719388

End: 2720872

Strand: Reverse

Name: purF [H]

Synonym: cg2857

Alternate gene names: 62391423

Gene position: 2720872-2719388 (Counterclockwise)

Preceding gene: 62391424

Following gene: 62391422

Centisome position: 82.88

GC content: 57.51

Gene sequence:

>1485_bases
ATGACCCAGGTAAACCACCCGTACGATGATCTCAACGAACAGGCACCGCAGGAAGAATGCGGCGTTTTCGGCGTTTGGGC
GCCAGGTGAGGAAGTCTCGAAACTTACCTACTTTGGCCTCTTCGCACTTCAGCACCGTGGTCAAGAAGCCGCGGGCATCG
CAGTAGGCGATGGCGAACAGATCCTGGTTTTCAAAGATTTGGGCCTAGTCTCCCAAGTTTTCGACGAACCAATTCTGGAA
TCCCTCCGCGGAAACATCGCCATCGGACACACCCGATACACCACCGCCGGCGGAAACACCTGGGAAAATGCCCAGCCTAT
GTTCCGCATGGCACCAGATGGCACCGATATCGCCCTTGGACACAACGGCAACCTGATTAATTACATCGAGTTGTTGGACA
AAGCCACCGAACTTGGCCTCGTCGATCCCGCCAAGAAGCCATCAGATACCGATGTGCTCACTGGACTGCTCGCAAGCGGC
GTCCATGACGGAAATAATCTCTTTGATTCCGCCAAGGAACTCCTCCCCAGCGTCAAGGGAGCCTACTGCCTCACCTTCAC
CGACGGACACACCCTGTACGCAGCGCGTGATCCATTCGGCATCCGCCCACTGTCCATCGGCCGCCTCGAGCGCGGCTGGG
TAGTCGCATCTGAAACCGCAGCGCTCGACATCGTAGGTGCCTCGCATGTGCGCGAGGTCGAACCAGGCGAACTGATTGCT
ATCGACGAATCCGGCCTCAAGTCCGCACGATTCGCCGAGACAACCCGCAAAGGTTGCGTCTTCGAATACGTTTACCTGGC
TCGTCCAGACTCCGTGATCAAGGGAAGAAACGTCAACGAAGCCCGACTTGAAATCGGCCGCAAGCTCGCTGCAGAAGCAC
CAGCAGTCGGCGATCTAGTCATCCCAACCCCAGAATCAGGCACCCCAGCAGCAGTTGGATTCGCCCAAGCATCTGGCATC
CCATTCGGCCAAGGCATGGTCAAAAACGCCTACGTTGGCCGAACCTTCATCCAGCCTTCCGACACTCTCCGCCAACTGGG
AATCCGCCTCAAGCTGAACCCATTGCGCGAGGTTATCGCCGGAAAGCGCCTTGTGGTTGTGGATGATTCCATCGTCCGCG
GTAACACCCAACGCGCCGTGATCCGCATGTTGCGCGAAGCCGGTGCAGCTGAGGTTCACGTACGCATCGCCTCACCACCC
GTGAAATGGCCATGCTTCTACGGCATCGATTTTGCCACCCCAGGCGAACTCATTGCCAACGCTGTCACCAGTGACAACGA
AGCAGAAATGGTAGAAGCAGTCCGCTCCGCAATCGGCGCAGACACCCTCGGCTACGTCTCCATCGACTCCATGGTTGCAG
CAACCGAGCAACCAGCCAACGAACTCTGCATCGCCTGCTTCGACGGCAAATACCCCATGGGTCTGCCACAGGGAAACAGC
AACGCAGACCTAGTCCGCAAGATGCAAGCAACCGCCTCAAGTTAA

Upstream 100 bases:

>100_bases
TTAGTTTCTGGGCAACCGACAGACTACGATTTGACCTGTGGCATTACTTGATCAGCACACGCTCCCTGGTTCAATGGTAA
ATACCGAGCATTCCACCGGA

Downstream 100 bases:

>100_bases
GATCGGTAGGCGATAGGGGTTGAGCATTTTTGCTCTCCCCGTATGCGTGGGGGTTGTCCCGCGCACTTTTAGACAGTTAT
GTTCCTAATAAGTTCCAAAC

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MTQVNHPYDDLNEQAPQEECGVFGVWAPGEEVSKLTYFGLFALQHRGQEAAGIAVGDGEQILVFKDLGLVSQVFDEPILE
SLRGNIAIGHTRYTTAGGNTWENAQPMFRMAPDGTDIALGHNGNLINYIELLDKATELGLVDPAKKPSDTDVLTGLLASG
VHDGNNLFDSAKELLPSVKGAYCLTFTDGHTLYAARDPFGIRPLSIGRLERGWVVASETAALDIVGASHVREVEPGELIA
IDESGLKSARFAETTRKGCVFEYVYLARPDSVIKGRNVNEARLEIGRKLAAEAPAVGDLVIPTPESGTPAAVGFAQASGI
PFGQGMVKNAYVGRTFIQPSDTLRQLGIRLKLNPLREVIAGKRLVVVDDSIVRGNTQRAVIRMLREAGAAEVHVRIASPP
VKWPCFYGIDFATPGELIANAVTSDNEAEMVEAVRSAIGADTLGYVSIDSMVAATEQPANELCIACFDGKYPMGLPQGNS
NADLVRKMQATASS

Sequences:

>Translated_494_residues
MTQVNHPYDDLNEQAPQEECGVFGVWAPGEEVSKLTYFGLFALQHRGQEAAGIAVGDGEQILVFKDLGLVSQVFDEPILE
SLRGNIAIGHTRYTTAGGNTWENAQPMFRMAPDGTDIALGHNGNLINYIELLDKATELGLVDPAKKPSDTDVLTGLLASG
VHDGNNLFDSAKELLPSVKGAYCLTFTDGHTLYAARDPFGIRPLSIGRLERGWVVASETAALDIVGASHVREVEPGELIA
IDESGLKSARFAETTRKGCVFEYVYLARPDSVIKGRNVNEARLEIGRKLAAEAPAVGDLVIPTPESGTPAAVGFAQASGI
PFGQGMVKNAYVGRTFIQPSDTLRQLGIRLKLNPLREVIAGKRLVVVDDSIVRGNTQRAVIRMLREAGAAEVHVRIASPP
VKWPCFYGIDFATPGELIANAVTSDNEAEMVEAVRSAIGADTLGYVSIDSMVAATEQPANELCIACFDGKYPMGLPQGNS
NADLVRKMQATASS
>Mature_493_residues
TQVNHPYDDLNEQAPQEECGVFGVWAPGEEVSKLTYFGLFALQHRGQEAAGIAVGDGEQILVFKDLGLVSQVFDEPILES
LRGNIAIGHTRYTTAGGNTWENAQPMFRMAPDGTDIALGHNGNLINYIELLDKATELGLVDPAKKPSDTDVLTGLLASGV
HDGNNLFDSAKELLPSVKGAYCLTFTDGHTLYAARDPFGIRPLSIGRLERGWVVASETAALDIVGASHVREVEPGELIAI
DESGLKSARFAETTRKGCVFEYVYLARPDSVIKGRNVNEARLEIGRKLAAEAPAVGDLVIPTPESGTPAAVGFAQASGIP
FGQGMVKNAYVGRTFIQPSDTLRQLGIRLKLNPLREVIAGKRLVVVDDSIVRGNTQRAVIRMLREAGAAEVHVRIASPPV
KWPCFYGIDFATPGELIANAVTSDNEAEMVEAVRSAIGADTLGYVSIDSMVAATEQPANELCIACFDGKYPMGLPQGNSN
ADLVRKMQATASS

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=524, Percent_Identity=39.6946564885496, Blast_Score=345, Evalue=8e-95,
Organism=Escherichia coli, GI1788651, Length=476, Percent_Identity=38.8655462184874, Blast_Score=282, Evalue=4e-77,
Organism=Escherichia coli, GI1790167, Length=192, Percent_Identity=29.6875, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17554892, Length=488, Percent_Identity=37.2950819672131, Blast_Score=293, Evalue=2e-79,
Organism=Saccharomyces cerevisiae, GI6323958, Length=486, Percent_Identity=32.7160493827161, Blast_Score=242, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24659598, Length=497, Percent_Identity=40.8450704225352, Blast_Score=365, Evalue=1e-101,
Organism=Drosophila melanogaster, GI28573187, Length=500, Percent_Identity=41.2, Blast_Score=358, Evalue=6e-99,
Organism=Drosophila melanogaster, GI24659604, Length=476, Percent_Identity=41.1764705882353, Blast_Score=353, Evalue=1e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 52877; Mature: 52745

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQVNHPYDDLNEQAPQEECGVFGVWAPGEEVSKLTYFGLFALQHRGQEAAGIAVGDGEQ
CCCCCCCHHHHHHCCCHHHCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCE
ILVFKDLGLVSQVFDEPILESLRGNIAIGHTRYTTAGGNTWENAQPMFRMAPDGTDIALG
EEEEECCHHHHHHHHHHHHHHHCCCEEECCEEEEECCCCCCCCCCCCEEECCCCCEEEEC
HNGNLINYIELLDKATELGLVDPAKKPSDTDVLTGLLASGVHDGNNLFDSAKELLPSVKG
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCC
AYCLTFTDGHTLYAARDPFGIRPLSIGRLERGWVVASETAALDIVGASHVREVEPGELIA
CEEEEEECCCEEEEECCCCCCCCCCCCCCCCCEEEEECCCEEEEECHHHHCCCCCCCEEE
IDESGLKSARFAETTRKGCVFEYVYLARPDSVIKGRNVNEARLEIGRKLAAEAPAVGDLV
ECCCCCCHHHHHHHHHCCCEEEEEEEECCCHHHCCCCCCHHHHHHHHHHHHCCCCCCCEE
IPTPESGTPAAVGFAQASGIPFGQGMVKNAYVGRTFIQPSDTLRQLGIRLKLNPLREVIA
ECCCCCCCCCEEEEHHHCCCCCCCCHHHCCCCCCEEECCHHHHHHCCCEEEECHHHHHHC
GKRLVVVDDSIVRGNTQRAVIRMLREAGAAEVHVRIASPPVKWPCFYGIDFATPGELIAN
CCEEEEECCHHHCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEECCCCCCCHHHHHH
AVTSDNEAEMVEAVRSAIGADTLGYVSIDSMVAATEQPANELCIACFDGKYPMGLPQGNS
HHCCCCHHHHHHHHHHHHCCCCCCEEEHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCC
NADLVRKMQATASS
CHHHHHHHHHHCCC
>Mature Secondary Structure 
TQVNHPYDDLNEQAPQEECGVFGVWAPGEEVSKLTYFGLFALQHRGQEAAGIAVGDGEQ
CCCCCCHHHHHHCCCHHHCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCE
ILVFKDLGLVSQVFDEPILESLRGNIAIGHTRYTTAGGNTWENAQPMFRMAPDGTDIALG
EEEEECCHHHHHHHHHHHHHHHCCCEEECCEEEEECCCCCCCCCCCCEEECCCCCEEEEC
HNGNLINYIELLDKATELGLVDPAKKPSDTDVLTGLLASGVHDGNNLFDSAKELLPSVKG
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCC
AYCLTFTDGHTLYAARDPFGIRPLSIGRLERGWVVASETAALDIVGASHVREVEPGELIA
CEEEEEECCCEEEEECCCCCCCCCCCCCCCCCEEEEECCCEEEEECHHHHCCCCCCCEEE
IDESGLKSARFAETTRKGCVFEYVYLARPDSVIKGRNVNEARLEIGRKLAAEAPAVGDLV
ECCCCCCHHHHHHHHHCCCEEEEEEEECCCHHHCCCCCCHHHHHHHHHHHHCCCCCCCEE
IPTPESGTPAAVGFAQASGIPFGQGMVKNAYVGRTFIQPSDTLRQLGIRLKLNPLREVIA
ECCCCCCCCCEEEEHHHCCCCCCCCHHHCCCCCCEEECCHHHHHHCCCEEEECHHHHHHC
GKRLVVVDDSIVRGNTQRAVIRMLREAGAAEVHVRIASPPVKWPCFYGIDFATPGELIAN
CCEEEEECCHHHCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEECCCCCCCHHHHHH
AVTSDNEAEMVEAVRSAIGADTLGYVSIDSMVAATEQPANELCIACFDGKYPMGLPQGNS
HHCCCCHHHHHHHHHHHHCCCCCCEEEHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCC
NADLVRKMQATASS
CHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]