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The map label for this gene is folC [H]

Identifier: 62391218

GI number: 62391218

Start: 2485787

End: 2487310

Strand: Reverse

Name: folC [H]

Synonym: cg2608

Alternate gene names: 62391218

Gene position: 2487310-2485787 (Counterclockwise)

Preceding gene: 62391219

Following gene: 62391217

Centisome position: 75.77

GC content: 57.55

Gene sequence:

>1524_bases
GTGGCTAACAACGACGGAATCTTTGATTCCGAAAATGATGACATCACCGTAGGCGATGTCACGTTGGGCGAGACTGGACT
GTCTTTGCCCATCGATCTCGCCGGTGAGGTAGAAGCACCTGCCTCCGAGGAGATCACCCAAGAAGATTTGCTGCGCCTTG
CCCAGGTGGAAGCAGAGTTGGATCAACGTTGGTTGGAAACCAAAATTGATCCCACTTTCCGACGCATGAGCTACATGATG
GATCTCATGGGCCAACCACAGAATTCCTTCCCAGCAATTCACGTGGCTGGCACCAACGGTAAGACCTCCACCACCCGCAT
GATCGAGTCGTTGCTGCGCGCATTCCACCGCCGCACCGGCCGGACCACCAGCCCGCACCTGCAGCTGGTAACCGAACGCA
TCGCGATTGATGGCAAGCCCATCCACCCGCGTGATTTCGTGCGGATCTACGAAGAGATTAAGCCCTACATGGAGATGACC
GACGCCTGGTCAGAGGCCGAGGGCGGACCGAAGATGAGCAAGTTTGAGGCACTCGTGGCCCTCGCTTACGCAGGTTTTGC
CGACGCTCCTGTTGACGTCGCCGTCGTTGAGGTTGGTCTTGGCGGACGCTGGGATGCCACTAACGTGATCAACGCAGCTG
TTTCCGTGATCACCCCGGTGGGCATGGACCACGTGGATCGCCTGGGCAACACCATTGGTGAAATCGCTGGTGAAAAGGCC
GGCATCATCAAGGCTCGTCCTGCATCTGAGGATGGCACCGAGCCTGAGGGCAACGTTGTCATCGTGGGCAAGCAGGAGCC
AGAAGCAATGAACGTGATTCTGCAGCAAGCCGTGGACGTGGACGCAGCTGTTGCTCGTTTGAACATGGAATTCGGCGTGG
TGGAATCCGCCATTGCCGTTGGTGGACAGCAGCTCACCCTGAAGGGTTTGGGCGGCGAATACACCGACATCTTCCTCCCA
CTGTCTGGCGCGCACCAAGCAGATAATGCCGCGGTTGCTCTCGCAGCAGTGGAAGCATTTTTCGGTGCATCCGCCGGACG
CCCATTGGATATCGACACGGTGCGCGAAGGCTTCGCACAAGTTCAGTCCCCAGGTCGCCTCGAGCGCCTGCGCTCTGCAC
CAACCGTGTTCATCGACGCAGCTCACAACCCACACGGTGCCGCAGCACTTGGTGCAGCACTAGACCGTGACTTTGAGTTC
CGTCGCCTCATCGGTGTCATCGGAGTGCTCTGCGACAAGGATGCCCGCGGCATCTTGGAATCACTTGAGCCATACCTGCA
TGAAATTGTGTGCACCCAGACTGCCTCAGAGCGCGCATTGGACGCATACGATTTAGCTGAATATGCTCGAGAGATCTACG
GCGATGAGCGTGTGCACGTCCAAGAAGATCTTGCTGGCGCGGTAGAACTCGCTATTGAACTAGCAGAAGACACCGATGTA
CAGTCCGGATCAGGTGTTGTGATCACCGGTTCAATCGTGACCGCCGGCGATGCGCGCACGCTGTTTGGAAAGGAACCTGC
ATGA

Upstream 100 bases:

>100_bases
CCAAGGCACCTGATGCAGTGGTAGACAAGATCCGTGGCCGTGCGCAGATTGCCCAAGAAGAAGTTGAGCGCATTAACAAG
CGTCTGGAGGAATTGGCCTA

Downstream 100 bases:

>100_bases
GCAAGCGTGAAGAATCAATTGAGTACGGACCATTAGGCAAAGGCCACGATCCATTAAAGGATCCCATGAAGGGTATCCGA
GGTGTCATGGCCGGCACCTT

Product: folylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 507; Mature: 506

Protein sequence:

>507_residues
MANNDGIFDSENDDITVGDVTLGETGLSLPIDLAGEVEAPASEEITQEDLLRLAQVEAELDQRWLETKIDPTFRRMSYMM
DLMGQPQNSFPAIHVAGTNGKTSTTRMIESLLRAFHRRTGRTTSPHLQLVTERIAIDGKPIHPRDFVRIYEEIKPYMEMT
DAWSEAEGGPKMSKFEALVALAYAGFADAPVDVAVVEVGLGGRWDATNVINAAVSVITPVGMDHVDRLGNTIGEIAGEKA
GIIKARPASEDGTEPEGNVVIVGKQEPEAMNVILQQAVDVDAAVARLNMEFGVVESAIAVGGQQLTLKGLGGEYTDIFLP
LSGAHQADNAAVALAAVEAFFGASAGRPLDIDTVREGFAQVQSPGRLERLRSAPTVFIDAAHNPHGAAALGAALDRDFEF
RRLIGVIGVLCDKDARGILESLEPYLHEIVCTQTASERALDAYDLAEYAREIYGDERVHVQEDLAGAVELAIELAEDTDV
QSGSGVVITGSIVTAGDARTLFGKEPA

Sequences:

>Translated_507_residues
MANNDGIFDSENDDITVGDVTLGETGLSLPIDLAGEVEAPASEEITQEDLLRLAQVEAELDQRWLETKIDPTFRRMSYMM
DLMGQPQNSFPAIHVAGTNGKTSTTRMIESLLRAFHRRTGRTTSPHLQLVTERIAIDGKPIHPRDFVRIYEEIKPYMEMT
DAWSEAEGGPKMSKFEALVALAYAGFADAPVDVAVVEVGLGGRWDATNVINAAVSVITPVGMDHVDRLGNTIGEIAGEKA
GIIKARPASEDGTEPEGNVVIVGKQEPEAMNVILQQAVDVDAAVARLNMEFGVVESAIAVGGQQLTLKGLGGEYTDIFLP
LSGAHQADNAAVALAAVEAFFGASAGRPLDIDTVREGFAQVQSPGRLERLRSAPTVFIDAAHNPHGAAALGAALDRDFEF
RRLIGVIGVLCDKDARGILESLEPYLHEIVCTQTASERALDAYDLAEYAREIYGDERVHVQEDLAGAVELAIELAEDTDV
QSGSGVVITGSIVTAGDARTLFGKEPA
>Mature_506_residues
ANNDGIFDSENDDITVGDVTLGETGLSLPIDLAGEVEAPASEEITQEDLLRLAQVEAELDQRWLETKIDPTFRRMSYMMD
LMGQPQNSFPAIHVAGTNGKTSTTRMIESLLRAFHRRTGRTTSPHLQLVTERIAIDGKPIHPRDFVRIYEEIKPYMEMTD
AWSEAEGGPKMSKFEALVALAYAGFADAPVDVAVVEVGLGGRWDATNVINAAVSVITPVGMDHVDRLGNTIGEIAGEKAG
IIKARPASEDGTEPEGNVVIVGKQEPEAMNVILQQAVDVDAAVARLNMEFGVVESAIAVGGQQLTLKGLGGEYTDIFLPL
SGAHQADNAAVALAAVEAFFGASAGRPLDIDTVREGFAQVQSPGRLERLRSAPTVFIDAAHNPHGAAALGAALDRDFEFR
RLIGVIGVLCDKDARGILESLEPYLHEIVCTQTASERALDAYDLAEYAREIYGDERVHVQEDLAGAVELAIELAEDTDVQ
SGSGVVITGSIVTAGDARTLFGKEPA

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=363, Percent_Identity=31.129476584022, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI66932984, Length=363, Percent_Identity=31.129476584022, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI1788654, Length=383, Percent_Identity=31.331592689295, Blast_Score=143, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI71984923, Length=341, Percent_Identity=30.4985337243402, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17553148, Length=341, Percent_Identity=29.9120234604106, Blast_Score=119, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17553150, Length=341, Percent_Identity=30.4985337243402, Blast_Score=118, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6323760, Length=455, Percent_Identity=29.6703296703297, Blast_Score=155, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6324815, Length=199, Percent_Identity=33.6683417085427, Blast_Score=101, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24641571, Length=193, Percent_Identity=37.8238341968912, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24581568, Length=200, Percent_Identity=35, Blast_Score=105, Evalue=9e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 54273; Mature: 54141

Theoretical pI: Translated: 4.23; Mature: 4.23

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANNDGIFDSENDDITVGDVTLGETGLSLPIDLAGEVEAPASEEITQEDLLRLAQVEAEL
CCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHH
DQRWLETKIDPTFRRMSYMMDLMGQPQNSFPAIHVAGTNGKTSTTRMIESLLRAFHRRTG
HHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCC
RTTSPHLQLVTERIAIDGKPIHPRDFVRIYEEIKPYMEMTDAWSEAEGGPKMSKFEALVA
CCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LAYAGFADAPVDVAVVEVGLGGRWDATNVINAAVSVITPVGMDHVDRLGNTIGEIAGEKA
HHHCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCC
GIIKARPASEDGTEPEGNVVIVGKQEPEAMNVILQQAVDVDAAVARLNMEFGVVESAIAV
CEEEECCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCHHHHHHHHCCHHHHHHHHHHC
GGQQLTLKGLGGEYTDIFLPLSGAHQADNAAVALAAVEAFFGASAGRPLDIDTVREGFAQ
CCCEEEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
VQSPGRLERLRSAPTVFIDAAHNPHGAAALGAALDRDFEFRRLIGVIGVLCDKDARGILE
HCCCHHHHHHHCCCEEEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHH
SLEPYLHEIVCTQTASERALDAYDLAEYAREIYGDERVHVQEDLAGAVELAIELAEDTDV
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHCCCCC
QSGSGVVITGSIVTAGDARTLFGKEPA
CCCCCEEEECCEEECCCCCCCCCCCCC
>Mature Secondary Structure 
ANNDGIFDSENDDITVGDVTLGETGLSLPIDLAGEVEAPASEEITQEDLLRLAQVEAEL
CCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHH
DQRWLETKIDPTFRRMSYMMDLMGQPQNSFPAIHVAGTNGKTSTTRMIESLLRAFHRRTG
HHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCC
RTTSPHLQLVTERIAIDGKPIHPRDFVRIYEEIKPYMEMTDAWSEAEGGPKMSKFEALVA
CCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LAYAGFADAPVDVAVVEVGLGGRWDATNVINAAVSVITPVGMDHVDRLGNTIGEIAGEKA
HHHCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCC
GIIKARPASEDGTEPEGNVVIVGKQEPEAMNVILQQAVDVDAAVARLNMEFGVVESAIAV
CEEEECCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCHHHHHHHHCCHHHHHHHHHHC
GGQQLTLKGLGGEYTDIFLPLSGAHQADNAAVALAAVEAFFGASAGRPLDIDTVREGFAQ
CCCEEEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
VQSPGRLERLRSAPTVFIDAAHNPHGAAALGAALDRDFEFRRLIGVIGVLCDKDARGILE
HCCCHHHHHHHCCCEEEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHH
SLEPYLHEIVCTQTASERALDAYDLAEYAREIYGDERVHVQEDLAGAVELAIELAEDTDV
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHCCCCC
QSGSGVVITGSIVTAGDARTLFGKEPA
CCCCCEEEECCEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]