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The map label for this gene is dnaG [H]

Identifier: 62391109

GI number: 62391109

Start: 2371077

End: 2372978

Strand: Reverse

Name: dnaG [H]

Synonym: cg2489

Alternate gene names: 62391109

Gene position: 2372978-2371077 (Counterclockwise)

Preceding gene: 62391112

Following gene: 62391104

Centisome position: 72.29

GC content: 54.78

Gene sequence:

>1902_bases
ATGGTCATGGCAAAGGGACGTATTCCGGAAAGTGACATTCAGGCAATCCGCGAGCGCACCCCGATCGAGGAGATCGTGGG
TGATTATGTGCAGCTGAAATCGGCAGGTGCTGATTCGCTCAAGGGGCTTTCTCCCTTTAAAGATGAAAAAACACCATCGT
TCCATGTGCGCCCCAACCGTGGTTACTACCACTGTTTCTCCACCGGCAAAGGTGGAGATGTGTTCTCCTTCCTCATGGAG
ATGGAACACATCTCTTTCCCTGAGGCTGTGGAAGTGTGTGCGGAGAAAATTGGTTATCAGATCAATTACCAAGGTGGTGG
CCCGGGGCGCCGTGAGGAACCTGGAACTCGCCAGCGCCTTATTTTGGCTAATAAGGCTGCGCACCAGTTTTATCGCGAGC
AATTAGAAACCCCCGAAGCACAACCTGGCAGGGAGTTTTTGCTGCAGCGTGGATTCGGACAGCAGCACATTTATCATTTC
GAATGTGGCTATGCGCCTGCCGGCTGGGATACCTTGACCAAGCATTTGCTGAAGAAGGGCTTTGAGTTCAAGGAATTAGA
AGCTGCCGGTCTAAGCAAGATGGGTAAGCGCGGTCCGATTGATCAGTTCCAGCGCAGGTTGCTGTGGCCGATCAAGAACC
TGTCTGGTGATGTCATTGGTTTTGGCGCCCGCAAGCTTTTCGATGACGACAAAATGGGCAAGTACATGAATACGCCTGAG
ACGTTGTTGTACAAAAAGTCCAAGGTGCTCTTTGGTCTAGATTCTGCAAAGAAGGCCATTGCAGCTGGCCACCAAGCAGT
AGTGGTGGAAGGCTACACCGATGTGATGGCCATGCATGCCGCGGGCATTGATACAGCCGTGGCATCGTGTGGCACTGCGT
TTGGTGAAGAACACTTGCAGATGCTTCGTCGACTCATGCTGGATGATAACTACTTCCGCGGTGAACTGATTTACACCTTC
GATGGTGATGAGGCCGGCCAGAAGGCCGCCATGCGTGCCTTTGAGGGCGATCAGAAGTTCACAGGACAATCATTTGTGTC
TGTGGCACCCAACGGCATGGATCCGTGTGATCTGCGCCTTGAGCGTGGCGATGCGGCGGTGCGTGATCTTGTGGCACGAC
GCATCCCGATGTTTGAGTTCGTCATCCAATCGATCATCAGCGAATACACCCTCGACACCGTGGAAGGCCGTCTGGCTGCG
CTTCGTCGGGCAGTCCCCATCGTGGCGGATATTCGCGATAAGACGCTGCAGTCTGAATACGCCCGCCTGCTGTCTGGTTG
GGTCGGCTGGTCTGATCCTTCAGAGGTGCTGCGTCAGGTTCACGAGGAAGCACGTCGCCCCAAGCGCGATAAGAAGCCTG
TGCGTGCAAAGCGTTTCGATCAACCGCTCGAGGATCAAAGCCTGCGACCCACCATGGCGCTGCCTAATCCGCGGAACCCT
GTGCTGTGGCAGGAACGGGAATCACTCAAGATCGCCCTGCAATATCCGGAGCTCGCGGGATCGTACTTTGATGGACTGCC
AACCGATAGCTTCACCAACCCTGCCTACCGCATGGTACGCGATGCCATTTCGGCTGCTGGGGGATGTGAACGTGCCCTCG
ATGGCACTGATTGGTTGCCTGCCGTATCGGAAAATATGACTGATATTTTGGGCACGTCGTTGGTGTCAGAGCTGGCGATG
GAACCCATCGAGGTGGAAGCGCAAGACCTGGAATCATATACCGATGGTGTGTTGTCCAGGCTGCAGGAAACACGAGTGGG
CAACCAGATCGCCATCTTGAAAGGACAGCTGCAAAGAATGCGTCCGTCTGAAGATGAGCAAGCCTACAACTCGCTGTTTT
CCGATCTGGTTGCCCTGGAACAGGCGCGCCGGGAGCTGTTGGCCCGGGCGTTTAGAGGGTAA

Upstream 100 bases:

>100_bases
CAGCAGCGAGGACAACGATGACTCCGAGCACTCCGCCGAGGGTCTTCTTGCTGTTTTGCATACAACCAATATAGGCACAC
TAAAGGGACGGGCTAAAGTG

Downstream 100 bases:

>100_bases
TTTAGTCCTGGTCTTGCTCGAGGACTTCATGCCCGTCCGCAGTCCGCAGGTTTTTTACTTCCTTCATGCGGGGCTGCGGA
TCCAGCTTGTTGGCAATGGC

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 633; Mature: 633

Protein sequence:

>633_residues
MVMAKGRIPESDIQAIRERTPIEEIVGDYVQLKSAGADSLKGLSPFKDEKTPSFHVRPNRGYYHCFSTGKGGDVFSFLME
MEHISFPEAVEVCAEKIGYQINYQGGGPGRREEPGTRQRLILANKAAHQFYREQLETPEAQPGREFLLQRGFGQQHIYHF
ECGYAPAGWDTLTKHLLKKGFEFKELEAAGLSKMGKRGPIDQFQRRLLWPIKNLSGDVIGFGARKLFDDDKMGKYMNTPE
TLLYKKSKVLFGLDSAKKAIAAGHQAVVVEGYTDVMAMHAAGIDTAVASCGTAFGEEHLQMLRRLMLDDNYFRGELIYTF
DGDEAGQKAAMRAFEGDQKFTGQSFVSVAPNGMDPCDLRLERGDAAVRDLVARRIPMFEFVIQSIISEYTLDTVEGRLAA
LRRAVPIVADIRDKTLQSEYARLLSGWVGWSDPSEVLRQVHEEARRPKRDKKPVRAKRFDQPLEDQSLRPTMALPNPRNP
VLWQERESLKIALQYPELAGSYFDGLPTDSFTNPAYRMVRDAISAAGGCERALDGTDWLPAVSENMTDILGTSLVSELAM
EPIEVEAQDLESYTDGVLSRLQETRVGNQIAILKGQLQRMRPSEDEQAYNSLFSDLVALEQARRELLARAFRG

Sequences:

>Translated_633_residues
MVMAKGRIPESDIQAIRERTPIEEIVGDYVQLKSAGADSLKGLSPFKDEKTPSFHVRPNRGYYHCFSTGKGGDVFSFLME
MEHISFPEAVEVCAEKIGYQINYQGGGPGRREEPGTRQRLILANKAAHQFYREQLETPEAQPGREFLLQRGFGQQHIYHF
ECGYAPAGWDTLTKHLLKKGFEFKELEAAGLSKMGKRGPIDQFQRRLLWPIKNLSGDVIGFGARKLFDDDKMGKYMNTPE
TLLYKKSKVLFGLDSAKKAIAAGHQAVVVEGYTDVMAMHAAGIDTAVASCGTAFGEEHLQMLRRLMLDDNYFRGELIYTF
DGDEAGQKAAMRAFEGDQKFTGQSFVSVAPNGMDPCDLRLERGDAAVRDLVARRIPMFEFVIQSIISEYTLDTVEGRLAA
LRRAVPIVADIRDKTLQSEYARLLSGWVGWSDPSEVLRQVHEEARRPKRDKKPVRAKRFDQPLEDQSLRPTMALPNPRNP
VLWQERESLKIALQYPELAGSYFDGLPTDSFTNPAYRMVRDAISAAGGCERALDGTDWLPAVSENMTDILGTSLVSELAM
EPIEVEAQDLESYTDGVLSRLQETRVGNQIAILKGQLQRMRPSEDEQAYNSLFSDLVALEQARRELLARAFRG
>Mature_633_residues
MVMAKGRIPESDIQAIRERTPIEEIVGDYVQLKSAGADSLKGLSPFKDEKTPSFHVRPNRGYYHCFSTGKGGDVFSFLME
MEHISFPEAVEVCAEKIGYQINYQGGGPGRREEPGTRQRLILANKAAHQFYREQLETPEAQPGREFLLQRGFGQQHIYHF
ECGYAPAGWDTLTKHLLKKGFEFKELEAAGLSKMGKRGPIDQFQRRLLWPIKNLSGDVIGFGARKLFDDDKMGKYMNTPE
TLLYKKSKVLFGLDSAKKAIAAGHQAVVVEGYTDVMAMHAAGIDTAVASCGTAFGEEHLQMLRRLMLDDNYFRGELIYTF
DGDEAGQKAAMRAFEGDQKFTGQSFVSVAPNGMDPCDLRLERGDAAVRDLVARRIPMFEFVIQSIISEYTLDTVEGRLAA
LRRAVPIVADIRDKTLQSEYARLLSGWVGWSDPSEVLRQVHEEARRPKRDKKPVRAKRFDQPLEDQSLRPTMALPNPRNP
VLWQERESLKIALQYPELAGSYFDGLPTDSFTNPAYRMVRDAISAAGGCERALDGTDWLPAVSENMTDILGTSLVSELAM
EPIEVEAQDLESYTDGVLSRLQETRVGNQIAILKGQLQRMRPSEDEQAYNSLFSDLVALEQARRELLARAFRG

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=437, Percent_Identity=32.7231121281465, Blast_Score=228, Evalue=8e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 71042; Mature: 71042

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVMAKGRIPESDIQAIRERTPIEEIVGDYVQLKSAGADSLKGLSPFKDEKTPSFHVRPNR
CCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCEEECCCC
GYYHCFSTGKGGDVFSFLMEMEHISFPEAVEVCAEKIGYQINYQGGGPGRREEPGTRQRL
CEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCEEEEECCCCCCCCCCCCCHHHH
ILANKAAHQFYREQLETPEAQPGREFLLQRGFGQQHIYHFECGYAPAGWDTLTKHLLKKG
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHCC
FEFKELEAAGLSKMGKRGPIDQFQRRLLWPIKNLSGDVIGFGARKLFDDDKMGKYMNTPE
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEECCHHHHCCCHHCCCCCCCCH
TLLYKKSKVLFGLDSAKKAIAAGHQAVVVEGYTDVMAMHAAGIDTAVASCGTAFGEEHLQ
HHHHHHCHHEEECCHHHHHHHCCCCEEEEECHHHHHHHHHCCHHHHHHHHHHHCCHHHHH
MLRRLMLDDNYFRGELIYTFDGDEAGQKAAMRAFEGDQKFTGQSFVSVAPNGMDPCDLRL
HHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCHHHEEE
ERGDAAVRDLVARRIPMFEFVIQSIISEYTLDTVEGRLAALRRAVPIVADIRDKTLQSEY
CCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
ARLLSGWVGWSDPSEVLRQVHEEARRPKRDKKPVRAKRFDQPLEDQSLRPTMALPNPRNP
HHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCC
VLWQERESLKIALQYPELAGSYFDGLPTDSFTNPAYRMVRDAISAAGGCERALDGTDWLP
CCCCCCCCEEEEEECHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCHHHCCCCCCCH
AVSENMTDILGTSLVSELAMEPIEVEAQDLESYTDGVLSRLQETRVGNQIAILKGQLQRM
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHC
RPSEDEQAYNSLFSDLVALEQARRELLARAFRG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVMAKGRIPESDIQAIRERTPIEEIVGDYVQLKSAGADSLKGLSPFKDEKTPSFHVRPNR
CCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCEEECCCC
GYYHCFSTGKGGDVFSFLMEMEHISFPEAVEVCAEKIGYQINYQGGGPGRREEPGTRQRL
CEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCEEEEECCCCCCCCCCCCCHHHH
ILANKAAHQFYREQLETPEAQPGREFLLQRGFGQQHIYHFECGYAPAGWDTLTKHLLKKG
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHCC
FEFKELEAAGLSKMGKRGPIDQFQRRLLWPIKNLSGDVIGFGARKLFDDDKMGKYMNTPE
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEECCHHHHCCCHHCCCCCCCCH
TLLYKKSKVLFGLDSAKKAIAAGHQAVVVEGYTDVMAMHAAGIDTAVASCGTAFGEEHLQ
HHHHHHCHHEEECCHHHHHHHCCCCEEEEECHHHHHHHHHCCHHHHHHHHHHHCCHHHHH
MLRRLMLDDNYFRGELIYTFDGDEAGQKAAMRAFEGDQKFTGQSFVSVAPNGMDPCDLRL
HHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCHHHEEE
ERGDAAVRDLVARRIPMFEFVIQSIISEYTLDTVEGRLAALRRAVPIVADIRDKTLQSEY
CCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
ARLLSGWVGWSDPSEVLRQVHEEARRPKRDKKPVRAKRFDQPLEDQSLRPTMALPNPRNP
HHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCC
VLWQERESLKIALQYPELAGSYFDGLPTDSFTNPAYRMVRDAISAAGGCERALDGTDWLP
CCCCCCCCEEEEEECHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCHHHCCCCCCCH
AVSENMTDILGTSLVSELAMEPIEVEAQDLESYTDGVLSRLQETRVGNQIAILKGQLQRM
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHC
RPSEDEQAYNSLFSDLVALEQARRELLARAFRG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9422594 [H]