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The map label for this gene is metF [H]

Identifier: 62391011

GI number: 62391011

Start: 2270121

End: 2271101

Strand: Reverse

Name: metF [H]

Synonym: cg2383

Alternate gene names: 62391011

Gene position: 2271101-2270121 (Counterclockwise)

Preceding gene: 62391014

Following gene: 62391009

Centisome position: 69.18

GC content: 53.52

Gene sequence:

>981_bases
ATGTCCCTAACGAACATCCCAGCCTCATCTCAATGGGCAATTAGCGACGTTTTGAAGCGTCCTTCACCCGGCCGAGTACC
TTTTTCTGTCGAGTTTATGCCACCCCGCGACGATGCAGCTGAAGAGCGTCTTTACCGCGCAGCAGAGGTCTTCCATGACC
TCGGTGCATCGTTTGTCTCCGTGACTTATGGTGCTGGCGGATCAACCCGTGAGAGAACCTCACGTATTGCTCGACGATTA
GCGAAACAACCGTTGACCACTCTGGTGCACCTGACCCTGGTTAACCACACTCGCGAAGAGATGAAGGCAATTCTTCGGGA
ATACCTAGAGCTGGGATTAACAAACCTGTTGGCGCTTCGAGGAGATCCGCCTGGAGACCCATTAGGCGATTGGGTGAGCA
CCGATGGAGGACTGAACTATGCCTCTGAGCTCATCGATCTTATTAAGTCCACTCCTGAGTTCCGGGAATTCGACCTCGGT
ATCGCCTCCTTCCCCGAAGGGCATTTCCGGGCGAAAACTCTAGAAGAAGACACCAAATACACTCTGGCGAAGCTGCGTGG
AGGGGCAGAGTACTCCATCACGCAGATGTTCTTTGATGTGGAAGACTACCTGCGACTTCGTGATCGCCTTGTCGCTGCAG
ACCCCATTCATGGTGCGAAGCCAATCATTCCTGGCATCATGCCCATTACGAGCCTGCGGTCTGTGCGTCGACAGGTCGAA
CTCTCTGGTGCTCAATTGCCGAGCCAACTAGAAGAATCACTTGTTCGAGCTGCAAACGGCAATGAAGAAGCGAACAAAGA
CGAGATCCGCAAGGTGGGCATTGAATATTCCACCAATATGGCAGAGCGACTCATTGCCGAAGGTGCGGAAGATCTGCACT
TCATGACGCTTAACTTCACCCGTGCAACCCAAGAAGTGTTGTACAACCTTGGCATGGCGCCTGCTTGGGGAGCAGAGCAC
GGCCAAGACGCGGTGCGTTAA

Upstream 100 bases:

>100_bases
TTCAATCGAATAGGGGGATGCGGGCTGAATTTTGGTGGAGGTGAATAAATGCCAGAGGCAGTCCCAACAAAACACTCTCA
TCACACTAAGATACCCAGGC

Downstream 100 bases:

>100_bases
GCCCTCTTAGGAATCATGAAGGGGGAGGGCGGTGATCAATACGGCAAACGGCCGTTGATCCCCGTCAAACCTAAACTGCC
TGAGCAAGTCAGTGAAGCCG

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MSLTNIPASSQWAISDVLKRPSPGRVPFSVEFMPPRDDAAEERLYRAAEVFHDLGASFVSVTYGAGGSTRERTSRIARRL
AKQPLTTLVHLTLVNHTREEMKAILREYLELGLTNLLALRGDPPGDPLGDWVSTDGGLNYASELIDLIKSTPEFREFDLG
IASFPEGHFRAKTLEEDTKYTLAKLRGGAEYSITQMFFDVEDYLRLRDRLVAADPIHGAKPIIPGIMPITSLRSVRRQVE
LSGAQLPSQLEESLVRAANGNEEANKDEIRKVGIEYSTNMAERLIAEGAEDLHFMTLNFTRATQEVLYNLGMAPAWGAEH
GQDAVR

Sequences:

>Translated_326_residues
MSLTNIPASSQWAISDVLKRPSPGRVPFSVEFMPPRDDAAEERLYRAAEVFHDLGASFVSVTYGAGGSTRERTSRIARRL
AKQPLTTLVHLTLVNHTREEMKAILREYLELGLTNLLALRGDPPGDPLGDWVSTDGGLNYASELIDLIKSTPEFREFDLG
IASFPEGHFRAKTLEEDTKYTLAKLRGGAEYSITQMFFDVEDYLRLRDRLVAADPIHGAKPIIPGIMPITSLRSVRRQVE
LSGAQLPSQLEESLVRAANGNEEANKDEIRKVGIEYSTNMAERLIAEGAEDLHFMTLNFTRATQEVLYNLGMAPAWGAEH
GQDAVR
>Mature_325_residues
SLTNIPASSQWAISDVLKRPSPGRVPFSVEFMPPRDDAAEERLYRAAEVFHDLGASFVSVTYGAGGSTRERTSRIARRLA
KQPLTTLVHLTLVNHTREEMKAILREYLELGLTNLLALRGDPPGDPLGDWVSTDGGLNYASELIDLIKSTPEFREFDLGI
ASFPEGHFRAKTLEEDTKYTLAKLRGGAEYSITQMFFDVEDYLRLRDRLVAADPIHGAKPIIPGIMPITSLRSVRRQVEL
SGAQLPSQLEESLVRAANGNEEANKDEIRKVGIEYSTNMAERLIAEGAEDLHFMTLNFTRATQEVLYNLGMAPAWGAEHG
QDAVR

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=301, Percent_Identity=35.2159468438538, Blast_Score=161, Evalue=7e-40,
Organism=Escherichia coli, GI1790377, Length=293, Percent_Identity=33.7883959044369, Blast_Score=165, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI25147441, Length=296, Percent_Identity=30.4054054054054, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI25147436, Length=296, Percent_Identity=30.4054054054054, Blast_Score=124, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6321313, Length=302, Percent_Identity=30.1324503311258, Blast_Score=131, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6325234, Length=294, Percent_Identity=30.6122448979592, Blast_Score=128, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 36227; Mature: 36096

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLTNIPASSQWAISDVLKRPSPGRVPFSVEFMPPRDDAAEERLYRAAEVFHDLGASFVS
CCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCHHHHE
VTYGAGGSTRERTSRIARRLAKQPLTTLVHLTLVNHTREEMKAILREYLELGLTNLLALR
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GDPPGDPLGDWVSTDGGLNYASELIDLIKSTPEFREFDLGIASFPEGHFRAKTLEEDTKY
CCCCCCCCCHHHCCCCCHHHHHHHHHHHHCCCCHHHHCCCHHCCCCCCCCHHHHHHHHHH
TLAKLRGGAEYSITQMFFDVEDYLRLRDRLVAADPIHGAKPIIPGIMPITSLRSVRRQVE
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
LSGAQLPSQLEESLVRAANGNEEANKDEIRKVGIEYSTNMAERLIAEGAEDLHFMTLNFT
HCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEEEHH
RATQEVLYNLGMAPAWGAEHGQDAVR
HHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SLTNIPASSQWAISDVLKRPSPGRVPFSVEFMPPRDDAAEERLYRAAEVFHDLGASFVS
CCCCCCCCCCHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCHHHHE
VTYGAGGSTRERTSRIARRLAKQPLTTLVHLTLVNHTREEMKAILREYLELGLTNLLALR
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GDPPGDPLGDWVSTDGGLNYASELIDLIKSTPEFREFDLGIASFPEGHFRAKTLEEDTKY
CCCCCCCCCHHHCCCCCHHHHHHHHHHHHCCCCHHHHCCCHHCCCCCCCCHHHHHHHHHH
TLAKLRGGAEYSITQMFFDVEDYLRLRDRLVAADPIHGAKPIIPGIMPITSLRSVRRQVE
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
LSGAQLPSQLEESLVRAANGNEEANKDEIRKVGIEYSTNMAERLIAEGAEDLHFMTLNFT
HCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEEEHH
RATQEVLYNLGMAPAWGAEHGQDAVR
HHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9515933 [H]