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The map label for this gene is hisH

Identifier: 62390932

GI number: 62390932

Start: 2182669

End: 2183304

Strand: Reverse

Name: hisH

Synonym: cg2300

Alternate gene names: 62390932

Gene position: 2183304-2182669 (Counterclockwise)

Preceding gene: 62390933

Following gene: 62390931

Centisome position: 66.51

GC content: 53.14

Gene sequence:

>636_bases
ATGACCAAAACTGTCGCCCTTCTCGACTACGGATCTGGAAACCTTCGTTCTGCTCAACGCGCACTAGAGCGTGCCGGTGC
AGAAGTTATCGTGAGCTCCGATCCAGAAGTTTGCACCAACGCTGATGGCCTCCTAGTTCCTGGAGTGGGCGCATTTGATG
CCTGCATGAAGGGTTTGAAAAACGTCTTCGGACATCGCATTATCGGACAGCGTCTTGCTGGTGGACGTCCAGTGATGGGT
ATTTGTGTGGGCATGCAGATCCTGTTCGATGAAGGCGATGAGCACGGCATTAAGTCAGCTGGTTGCGGCGAGTGGCCTGG
CAAAGTGGAACGCCTCCAAGCGGAGATCCTGCCTCACATGGGGTGGAACACACTTGAAATGCCTACCAACTCACCAATGT
TTGAGGGAATTTCACCTGATGAGCGTTTCTACTTCGTGCACTCCTATGGTGTGCGCAAGTGGACGTTGGAAACCGACGAT
CTGACCACGCCTCCAGAGGTTGTGTGGGCGAAGCACGAAAATGATCGTTTTGTGGCAGCTGTGGAAAACGGCACGCTGTG
GGCTACTCAATTCCACCCAGAAAAATCAGGTGACGCAGGCGCACAGCTACTGCGAAACTGGATCAACTACATCTAA

Upstream 100 bases:

>100_bases
ATCGCAGCTAGGCCAGTGTGGTGGATATAAAACCCTTTTGGGGAGAAAGAAACTCGACTGCGGTTCTTGATCCTGAAAGC
ACGTGACATAAACTATCGGC

Downstream 100 bases:

>100_bases
CAGATAGGATCAATATTCATGACCTTCACTATTCTTCCTGCAGTCGATGTAGTTAACGGACAAGCAGTTCGCCTAGATCA
GGGCGAGGCCGGCACTGAAA

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MTKTVALLDYGSGNLRSAQRALERAGAEVIVSSDPEVCTNADGLLVPGVGAFDACMKGLKNVFGHRIIGQRLAGGRPVMG
ICVGMQILFDEGDEHGIKSAGCGEWPGKVERLQAEILPHMGWNTLEMPTNSPMFEGISPDERFYFVHSYGVRKWTLETDD
LTTPPEVVWAKHENDRFVAAVENGTLWATQFHPEKSGDAGAQLLRNWINYI

Sequences:

>Translated_211_residues
MTKTVALLDYGSGNLRSAQRALERAGAEVIVSSDPEVCTNADGLLVPGVGAFDACMKGLKNVFGHRIIGQRLAGGRPVMG
ICVGMQILFDEGDEHGIKSAGCGEWPGKVERLQAEILPHMGWNTLEMPTNSPMFEGISPDERFYFVHSYGVRKWTLETDD
LTTPPEVVWAKHENDRFVAAVENGTLWATQFHPEKSGDAGAQLLRNWINYI
>Mature_210_residues
TKTVALLDYGSGNLRSAQRALERAGAEVIVSSDPEVCTNADGLLVPGVGAFDACMKGLKNVFGHRIIGQRLAGGRPVMGI
CVGMQILFDEGDEHGIKSAGCGEWPGKVERLQAEILPHMGWNTLEMPTNSPMFEGISPDERFYFVHSYGVRKWTLETDDL
TTPPEVVWAKHENDRFVAAVENGTLWATQFHPEKSGDAGAQLLRNWINYI

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=208, Percent_Identity=36.5384615384615, Blast_Score=120, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6319725, Length=214, Percent_Identity=35.0467289719626, Blast_Score=121, Evalue=8e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_CORGL (O69043)

Other databases:

- EMBL:   AF060558
- EMBL:   BA000036
- EMBL:   BX927154
- RefSeq:   NP_601296.1
- RefSeq:   YP_226334.1
- ProteinModelPortal:   O69043
- SMR:   O69043
- GeneID:   1020048
- GeneID:   3343712
- GenomeReviews:   BA000036_GR
- GenomeReviews:   BX927147_GR
- KEGG:   cgb:cg2300
- KEGG:   cgl:NCgl2016
- HOGENOM:   HBG292341
- OMA:   RPFFGIC
- PhylomeDB:   O69043
- ProtClustDB:   PRK13146
- BioCyc:   CGLU196627:CG2300-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 23174; Mature: 23042

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 82-82 ACT_SITE 192-192 ACT_SITE 194-194

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTVALLDYGSGNLRSAQRALERAGAEVIVSSDPEVCTNADGLLVPGVGAFDACMKGLK
CCCEEEEEECCCCCHHHHHHHHHHCCCEEEEECCCCHHCCCCCEEECCCCHHHHHHHHHH
NVFGHRIIGQRLAGGRPVMGICVGMQILFDEGDEHGIKSAGCGEWPGKVERLQAEILPHM
HHHHHHHHHHHHCCCCCHHHHHHCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
GWNTLEMPTNSPMFEGISPDERFYFVHSYGVRKWTLETDDLTTPPEVVWAKHENDRFVAA
CCCEEECCCCCCCCCCCCCCCEEEEEEECCCEEEEEECCCCCCCCCEEEEECCCCEEEEE
VENGTLWATQFHPEKSGDAGAQLLRNWINYI
EECCEEEEEEECCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TKTVALLDYGSGNLRSAQRALERAGAEVIVSSDPEVCTNADGLLVPGVGAFDACMKGLK
CCEEEEEECCCCCHHHHHHHHHHCCCEEEEECCCCHHCCCCCEEECCCCHHHHHHHHHH
NVFGHRIIGQRLAGGRPVMGICVGMQILFDEGDEHGIKSAGCGEWPGKVERLQAEILPHM
HHHHHHHHHHHHCCCCCHHHHHHCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
GWNTLEMPTNSPMFEGISPDERFYFVHSYGVRKWTLETDDLTTPPEVVWAKHENDRFVAA
CCCEEECCCCCCCCCCCCCCCEEEEEEECCCEEEEEECCCCCCCCCEEEEECCCCEEEEE
VENGTLWATQFHPEKSGDAGAQLLRNWINYI
EECCEEEEEEECCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12948626