Click here to switch to the map view.

The map label for this gene is pyk [H]

Identifier: 62390924

GI number: 62390924

Start: 2175696

End: 2177129

Strand: Reverse

Name: pyk [H]

Synonym: cg2291

Alternate gene names: 62390924

Gene position: 2177129-2175696 (Counterclockwise)

Preceding gene: 62390925

Following gene: 62390923

Centisome position: 66.32

GC content: 55.72

Gene sequence:

>1434_bases
ATGGGCGTGGATAGACGAACTAAGATTGTATGTACCCTAGGCCCAGCGGTGGCTAGTGCAGATGGAATTCTGCGTTTGGT
AGAAGACGGCATGGATGTTGCTCGCCTCAACTTCTCCCATGGTGACCACCCAGATCATGAGCAAAACTACAAGTGGGTCC
GCGAGGCGGCGGAGAAGACTGGCCGTGCAGTCGGTATTCTCGCAGACCTCCAAGGACCGAAGATCCGTCTTGGCCGTTTC
ACTGACGGCGCAACCGTGTGGGAAAACGGCGAGACCATTCGGATCACCGTTGACGATGTAGAGGGAACGCACGATCGTGT
GTCCACCACCTACAAGAATCTGGCAAAAGACGCGAAGCCAGGCGACCGCCTGCTCGTTGATGACGGCAAGGTTGGCCTCG
TCTGCGTTTCCGTCGAAGGTAACGACGTCATCTGTGAGGTTGTTGAGGGCGGACCAGTCTCCAACAACAAGGGTGTTTCC
CTGCCAGGTATGGATATTTCCGTACCTGCACTGTCCGAAAAGGATATCCGTGACCTGCGCTTCGCCCTGAAGCTCGGCGT
GGACTTTATTGCACTGTCCTTCGTACGTTCCCCAGCAGATGCTGAACTCGTTCACAAGATCATGGACGAAGAAGGTCGTC
GTGTTCCTGTGATCGCCAAGCTGGAAAAGCCAGAGGCTGTCACCTCCCTCGAGCCAATCGTGTTGGCATTCGACGCCGTC
ATGGTTGCTCGTGGTGACCTCGGCGTTGAGGTTCCTCTGGAGGAGGTTCCACTGGTTCAGAAGCGCGCAATCCAGATTGC
CCGTGAGAACGCAAAGCCAGTTATCGTGGCAACCCAGATGCTGGATTCCATGATTGAGAACTCCCGCCCAACCCGTGCGG
AAGCTTCTGACGTGGCAAACGCTGTGCTCGATGGCGCAGATGCTGTCATGCTTTCTGGTGAAACTTCAGTGGGCAAAGAT
CCGCACAACGTTGTGCGCACCATGTCTCGCATTGTTCGCTTCGCTGAAACCGACGGTCGCGTCCCAGACCTGACCCACAT
CCCTCGCACTAAGCGTGGCGTTATTTCCTACTCTGCACGTGATATCGCCGAGCGCCTCAACGCTCGTGCATTGGTTGCGT
TCACCACCTCTGGTGATACCGCAAAGCGTGTGGCTCGTCTGCACAGCCACCTGCCACTGCTCGTGTTCACTCCAAATGAG
GCAGTTCGCTCTGAGCTGGCGCTGACCTGGGGTGCAACCACCTTCCTGTGTCCACCTGTCAGCGATACCGATGACATGAT
GCGCGAAGTCGACCGTGCTCTTTTAGCAATGCCTGAGTACAACAAGGGTGACATGATGGTTGTTGTTGCAGGTTCCCCTC
CTGGTGTTACCGGTAACACCAACATGATTCACGTCCACCTTCTTGGTGACGACACAAGGATTGCAAAGCTCTAA

Upstream 100 bases:

>100_bases
CAGAGGCGTAGAACATTGTCTGTTCACACTCTGGGTCGCAAGATTCATCGAGAATTAATGGTAGTACCTGTGGCTTGAGG
GGGAATGACGTACTAGGCTT

Downstream 100 bases:

>100_bases
TCGCTTAAATCTTTCAAAAAATGCGTTGACACTGTACGTGGTGTCAGCGCATTTTTCGTGGCTAGGGCAGGGGAAATCCG
CCTCATTTAAGAATTTACTT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MGVDRRTKIVCTLGPAVASADGILRLVEDGMDVARLNFSHGDHPDHEQNYKWVREAAEKTGRAVGILADLQGPKIRLGRF
TDGATVWENGETIRITVDDVEGTHDRVSTTYKNLAKDAKPGDRLLVDDGKVGLVCVSVEGNDVICEVVEGGPVSNNKGVS
LPGMDISVPALSEKDIRDLRFALKLGVDFIALSFVRSPADAELVHKIMDEEGRRVPVIAKLEKPEAVTSLEPIVLAFDAV
MVARGDLGVEVPLEEVPLVQKRAIQIARENAKPVIVATQMLDSMIENSRPTRAEASDVANAVLDGADAVMLSGETSVGKD
PHNVVRTMSRIVRFAETDGRVPDLTHIPRTKRGVISYSARDIAERLNARALVAFTTSGDTAKRVARLHSHLPLLVFTPNE
AVRSELALTWGATTFLCPPVSDTDDMMREVDRALLAMPEYNKGDMMVVVAGSPPGVTGNTNMIHVHLLGDDTRIAKL

Sequences:

>Translated_477_residues
MGVDRRTKIVCTLGPAVASADGILRLVEDGMDVARLNFSHGDHPDHEQNYKWVREAAEKTGRAVGILADLQGPKIRLGRF
TDGATVWENGETIRITVDDVEGTHDRVSTTYKNLAKDAKPGDRLLVDDGKVGLVCVSVEGNDVICEVVEGGPVSNNKGVS
LPGMDISVPALSEKDIRDLRFALKLGVDFIALSFVRSPADAELVHKIMDEEGRRVPVIAKLEKPEAVTSLEPIVLAFDAV
MVARGDLGVEVPLEEVPLVQKRAIQIARENAKPVIVATQMLDSMIENSRPTRAEASDVANAVLDGADAVMLSGETSVGKD
PHNVVRTMSRIVRFAETDGRVPDLTHIPRTKRGVISYSARDIAERLNARALVAFTTSGDTAKRVARLHSHLPLLVFTPNE
AVRSELALTWGATTFLCPPVSDTDDMMREVDRALLAMPEYNKGDMMVVVAGSPPGVTGNTNMIHVHLLGDDTRIAKL
>Mature_476_residues
GVDRRTKIVCTLGPAVASADGILRLVEDGMDVARLNFSHGDHPDHEQNYKWVREAAEKTGRAVGILADLQGPKIRLGRFT
DGATVWENGETIRITVDDVEGTHDRVSTTYKNLAKDAKPGDRLLVDDGKVGLVCVSVEGNDVICEVVEGGPVSNNKGVSL
PGMDISVPALSEKDIRDLRFALKLGVDFIALSFVRSPADAELVHKIMDEEGRRVPVIAKLEKPEAVTSLEPIVLAFDAVM
VARGDLGVEVPLEEVPLVQKRAIQIARENAKPVIVATQMLDSMIENSRPTRAEASDVANAVLDGADAVMLSGETSVGKDP
HNVVRTMSRIVRFAETDGRVPDLTHIPRTKRGVISYSARDIAERLNARALVAFTTSGDTAKRVARLHSHLPLLVFTPNEA
VRSELALTWGATTFLCPPVSDTDDMMREVDRALLAMPEYNKGDMMVVVAGSPPGVTGNTNMIHVHLLGDDTRIAKL

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=489, Percent_Identity=38.0368098159509, Blast_Score=295, Evalue=6e-80,
Organism=Homo sapiens, GI33286422, Length=487, Percent_Identity=37.9876796714579, Blast_Score=294, Evalue=1e-79,
Organism=Homo sapiens, GI33286420, Length=487, Percent_Identity=37.9876796714579, Blast_Score=294, Evalue=1e-79,
Organism=Homo sapiens, GI32967597, Length=489, Percent_Identity=36.8098159509202, Blast_Score=274, Evalue=1e-73,
Organism=Homo sapiens, GI10835121, Length=489, Percent_Identity=36.8098159509202, Blast_Score=274, Evalue=2e-73,
Organism=Homo sapiens, GI310128732, Length=287, Percent_Identity=38.6759581881533, Blast_Score=198, Evalue=1e-50,
Organism=Homo sapiens, GI310128730, Length=287, Percent_Identity=38.6759581881533, Blast_Score=198, Evalue=1e-50,
Organism=Homo sapiens, GI310128736, Length=235, Percent_Identity=36.5957446808511, Blast_Score=158, Evalue=8e-39,
Organism=Homo sapiens, GI310128734, Length=235, Percent_Identity=36.5957446808511, Blast_Score=158, Evalue=8e-39,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=35.981308411215, Blast_Score=139, Evalue=8e-33,
Organism=Escherichia coli, GI1787965, Length=471, Percent_Identity=40.9766454352442, Blast_Score=335, Evalue=4e-93,
Organism=Escherichia coli, GI1788160, Length=475, Percent_Identity=39.7894736842105, Blast_Score=297, Evalue=1e-81,
Organism=Caenorhabditis elegans, GI17544584, Length=419, Percent_Identity=39.618138424821, Blast_Score=302, Evalue=2e-82,
Organism=Caenorhabditis elegans, GI17506829, Length=482, Percent_Identity=39.6265560165975, Blast_Score=285, Evalue=3e-77,
Organism=Caenorhabditis elegans, GI71984413, Length=482, Percent_Identity=39.6265560165975, Blast_Score=285, Evalue=3e-77,
Organism=Caenorhabditis elegans, GI71984406, Length=482, Percent_Identity=39.6265560165975, Blast_Score=285, Evalue=3e-77,
Organism=Caenorhabditis elegans, GI17506831, Length=482, Percent_Identity=39.6265560165975, Blast_Score=284, Evalue=8e-77,
Organism=Saccharomyces cerevisiae, GI6319279, Length=481, Percent_Identity=36.5904365904366, Blast_Score=264, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6324923, Length=487, Percent_Identity=33.4702258726899, Blast_Score=247, Evalue=3e-66,
Organism=Drosophila melanogaster, GI28571814, Length=486, Percent_Identity=37.037037037037, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI24648964, Length=489, Percent_Identity=37.2188139059305, Blast_Score=283, Evalue=3e-76,
Organism=Drosophila melanogaster, GI24581235, Length=491, Percent_Identity=32.7902240325866, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24648966, Length=410, Percent_Identity=36.0975609756098, Blast_Score=248, Evalue=8e-66,
Organism=Drosophila melanogaster, GI24646914, Length=276, Percent_Identity=35.1449275362319, Blast_Score=142, Evalue=5e-34,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51628; Mature: 51497

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVDRRTKIVCTLGPAVASADGILRLVEDGMDVARLNFSHGDHPDHEQNYKWVREAAEKT
CCCCCCEEEEEEECCHHHCHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHC
GRAVGILADLQGPKIRLGRFTDGATVWENGETIRITVDDVEGTHDRVSTTYKNLAKDAKP
CCEEEEEEECCCCEEEEEEECCCCEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCC
GDRLLVDDGKVGLVCVSVEGNDVICEVVEGGPVSNNKGVSLPGMDISVPALSEKDIRDLR
CCEEEEECCCEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCEECCCCCCCHHHHHH
FALKLGVDFIALSFVRSPADAELVHKIMDEEGRRVPVIAKLEKPEAVTSLEPIVLAFDAV
HHHHHCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHCCCHHHHHHHHH
MVARGDLGVEVPLEEVPLVQKRAIQIARENAKPVIVATQMLDSMIENSRPTRAEASDVAN
HEECCCCCCCCCHHHCCHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCCCCCHHHHHHH
AVLDGADAVMLSGETSVGKDPHNVVRTMSRIVRFAETDGRVPDLTHIPRTKRGVISYSAR
HHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEHHH
DIAERLNARALVAFTTSGDTAKRVARLHSHLPLLVFTPNEAVRSELALTWGATTFLCPPV
HHHHHCCCEEEEEEECCCHHHHHHHHHHHCCCEEEECCCHHHHHHEEEEECCCEEECCCC
SDTDDMMREVDRALLAMPEYNKGDMMVVVAGSPPGVTGNTNMIHVHLLGDDTRIAKL
CCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEEECCCCEECCC
>Mature Secondary Structure 
GVDRRTKIVCTLGPAVASADGILRLVEDGMDVARLNFSHGDHPDHEQNYKWVREAAEKT
CCCCCEEEEEEECCHHHCHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHC
GRAVGILADLQGPKIRLGRFTDGATVWENGETIRITVDDVEGTHDRVSTTYKNLAKDAKP
CCEEEEEEECCCCEEEEEEECCCCEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCC
GDRLLVDDGKVGLVCVSVEGNDVICEVVEGGPVSNNKGVSLPGMDISVPALSEKDIRDLR
CCEEEEECCCEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCEECCCCCCCHHHHHH
FALKLGVDFIALSFVRSPADAELVHKIMDEEGRRVPVIAKLEKPEAVTSLEPIVLAFDAV
HHHHHCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHCCCHHHHHHHHH
MVARGDLGVEVPLEEVPLVQKRAIQIARENAKPVIVATQMLDSMIENSRPTRAEASDVAN
HEECCCCCCCCCHHHCCHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCCCCCHHHHHHH
AVLDGADAVMLSGETSVGKDPHNVVRTMSRIVRFAETDGRVPDLTHIPRTKRGVISYSAR
HHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEHHH
DIAERLNARALVAFTTSGDTAKRVARLHSHLPLLVFTPNEAVRSELALTWGATTFLCPPV
HHHHHCCCEEEEEEECCCHHHHHHHHHHHCCCEEEECCCHHHHHHEEEEECCCEEECCCC
SDTDDMMREVDRALLAMPEYNKGDMMVVVAGSPPGVTGNTNMIHVHLLGDDTRIAKL
CCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEEECCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8074528; 12948626 [H]