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The map label for this gene is amtB [H]

Identifier: 62390898

GI number: 62390898

Start: 2142201

End: 2143517

Strand: Reverse

Name: amtB [H]

Synonym: cg2261

Alternate gene names: 62390898

Gene position: 2143517-2142201 (Counterclockwise)

Preceding gene: 62390899

Following gene: 62390897

Centisome position: 65.3

GC content: 54.97

Gene sequence:

>1317_bases
ATGGGTGCAGATCAAATTGCAGCAGTCTCCGGCAATTCAGCTTGGATGCTGATGTCCGCGTCGCTCGTGCTGCTAATGAC
ACCAGCACTGGCACTTTTCTATGGCGGCATGTCTCGTCAAAAGTCCGTGCTCAACATGATGATGATGTCCTTTGGAGCAT
TGGGCGTCGTTACTGTTATTTACCTCTTGTGGGGATGGTCGATGTCTTATGGAACCCAATCAATCGCGGGAATCTTTGCT
AACCCTTTTGAGTTCTTCGGTCTTAAAGATTCCATCGTTGATGCAGACGGCAACTACATTGAGGGCGCAGCTGGCTACCC
AAACATTATTGATATCGGATTCCAGCTTACCTTCGCCGTTATCTCCACTGCACTGATCTCCGGTGCGTTGGCAGAGCGTG
TGAAGTTTTCCACCTGGCTTATTTTCAGCGGCGCGTGGGTGACTTTGGTGTACTTCCCACTGGCACACATGGTGTGGGGT
GGCGGATTGTTGGGCCATAATGTCACTGGTTTTGCTTCGTGGTTGTTTGGTTCTACCGATGGTGAAGCCAATATCGCTCC
GATCGACTTTGCTGGTGGTACCGTTGTTCACATTTCGGCGGGCACAGCGGCCCTTGTGCTGGCTTTTATCGTCGGCAAGC
GAAAGACGTTTGGCAAGGCAATTGCCAGGCCGCACAACCTGCCCATGGTCATGCTCGGCGCTGCGCTGTTGTGGTTTGGT
TGGTTCGGGTTCAACGGTGGCTCGGCGTTCGCAGCCGACGGCCTTGCGGGCCTGGCCTGGGTGAACACCACGGCGGCAAC
CGCAGCTGCGATGCTTGGCTGGTTGGCTACTGAAAAATTCCGCGATGGACACGCAACCTCCTTGGGTGCTGCTTCTGGTG
TCGTCGCCGGTCTGGTCGCAATTACTCCAGCAGCTGGCGCACTGACCCCAGTGACCTCCCTGATCCTCGGTGCAATCGGC
GGCATCTTGGCGTGCTTGGGCGTGGGGCTGAAGTACCGCTTCGGTTTCGATGACTCCCTCGACGTAGTTGGTGTGCACCT
GGTCGCTGGCCTGTGGGGCACCGTGGGTGTAGGACTTCTTGCCACTGACATCGGATGGTTTAGTGGTGGTGGCATGGATG
GACTTAAACTCTTTATTGTCCAGATCGTCATCGCGCTAGTCGCTGTAGTTTTCGCAGGTGTGATTACTGCGCTCATCGCA
TTCGCCCTGAAGGCCACAGTAGGATGGCGCGTTGATGATGATGTGGAGCAGCAAGGCATTGATACTCACGAACACGCAGA
ATCCGCTTATGACACCACGGGTCCGGAAATTCGTTAA

Upstream 100 bases:

>100_bases
TAGTTAGAGCTGGTTCAAGGGGTGTCAATCCCAAAAGGCACTCCTTGAACTCATGAAAAAGCTTGACAAAACTTCAACGT
CAAAGGAGGTCATCCACGCT

Downstream 100 bases:

>100_bases
GTTTGTTTAAAAATACCTAAACCACTCTGACATTATTCCGAATGTCTTTTTGAAGGAGACGATCATGAAACTCATCACCG
CAATTGTCAAGCCGTTTACC

Product: low affinity ammonium uptake protein

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MGADQIAAVSGNSAWMLMSASLVLLMTPALALFYGGMSRQKSVLNMMMMSFGALGVVTVIYLLWGWSMSYGTQSIAGIFA
NPFEFFGLKDSIVDADGNYIEGAAGYPNIIDIGFQLTFAVISTALISGALAERVKFSTWLIFSGAWVTLVYFPLAHMVWG
GGLLGHNVTGFASWLFGSTDGEANIAPIDFAGGTVVHISAGTAALVLAFIVGKRKTFGKAIARPHNLPMVMLGAALLWFG
WFGFNGGSAFAADGLAGLAWVNTTAATAAAMLGWLATEKFRDGHATSLGAASGVVAGLVAITPAAGALTPVTSLILGAIG
GILACLGVGLKYRFGFDDSLDVVGVHLVAGLWGTVGVGLLATDIGWFSGGGMDGLKLFIVQIVIALVAVVFAGVITALIA
FALKATVGWRVDDDVEQQGIDTHEHAESAYDTTGPEIR

Sequences:

>Translated_438_residues
MGADQIAAVSGNSAWMLMSASLVLLMTPALALFYGGMSRQKSVLNMMMMSFGALGVVTVIYLLWGWSMSYGTQSIAGIFA
NPFEFFGLKDSIVDADGNYIEGAAGYPNIIDIGFQLTFAVISTALISGALAERVKFSTWLIFSGAWVTLVYFPLAHMVWG
GGLLGHNVTGFASWLFGSTDGEANIAPIDFAGGTVVHISAGTAALVLAFIVGKRKTFGKAIARPHNLPMVMLGAALLWFG
WFGFNGGSAFAADGLAGLAWVNTTAATAAAMLGWLATEKFRDGHATSLGAASGVVAGLVAITPAAGALTPVTSLILGAIG
GILACLGVGLKYRFGFDDSLDVVGVHLVAGLWGTVGVGLLATDIGWFSGGGMDGLKLFIVQIVIALVAVVFAGVITALIA
FALKATVGWRVDDDVEQQGIDTHEHAESAYDTTGPEIR
>Mature_437_residues
GADQIAAVSGNSAWMLMSASLVLLMTPALALFYGGMSRQKSVLNMMMMSFGALGVVTVIYLLWGWSMSYGTQSIAGIFAN
PFEFFGLKDSIVDADGNYIEGAAGYPNIIDIGFQLTFAVISTALISGALAERVKFSTWLIFSGAWVTLVYFPLAHMVWGG
GLLGHNVTGFASWLFGSTDGEANIAPIDFAGGTVVHISAGTAALVLAFIVGKRKTFGKAIARPHNLPMVMLGAALLWFGW
FGFNGGSAFAADGLAGLAWVNTTAATAAAMLGWLATEKFRDGHATSLGAASGVVAGLVAITPAAGALTPVTSLILGAIGG
ILACLGVGLKYRFGFDDSLDVVGVHLVAGLWGTVGVGLLATDIGWFSGGGMDGLKLFIVQIVIALVAVVFAGVITALIAF
ALKATVGWRVDDDVEQQGIDTHEHAESAYDTTGPEIR

Specific function: Involved in the uptake of ammonia (Probable) [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=427, Percent_Identity=40.7494145199063, Blast_Score=274, Evalue=8e-75,
Organism=Caenorhabditis elegans, GI17550182, Length=452, Percent_Identity=29.4247787610619, Blast_Score=131, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI17550180, Length=446, Percent_Identity=26.6816143497758, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI32564042, Length=450, Percent_Identity=24.6666666666667, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI133931100, Length=458, Percent_Identity=24.0174672489083, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6325396, Length=443, Percent_Identity=30.0225733634311, Blast_Score=192, Evalue=7e-50,
Organism=Saccharomyces cerevisiae, GI6324187, Length=439, Percent_Identity=30.751708428246, Blast_Score=181, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6321559, Length=442, Percent_Identity=27.8280542986425, Blast_Score=175, Evalue=1e-44,
Organism=Drosophila melanogaster, GI161078314, Length=443, Percent_Identity=26.6365688487585, Blast_Score=115, Evalue=9e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 45406; Mature: 45275

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGADQIAAVSGNSAWMLMSASLVLLMTPALALFYGGMSRQKSVLNMMMMSFGALGVVTVI
CCCCCEEEECCCCEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHH
YLLWGWSMSYGTQSIAGIFANPFEFFGLKDSIVDADGNYIEGAAGYPNIIDIGFQLTFAV
HHHHCCCHHCCCHHHHHHHCCCHHHCCCCHHHCCCCCCEECCCCCCCCEEHHHHHHHHHH
ISTALISGALAERVKFSTWLIFSGAWVTLVYFPLAHMVWGGGLLGHNVTGFASWLFGSTD
HHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCC
GEANIAPIDFAGGTVVHISAGTAALVLAFIVGKRKTFGKAIARPHNLPMVMLGAALLWFG
CCCCEEEEEECCCEEEEEECCHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHH
WFGFNGGSAFAADGLAGLAWVNTTAATAAAMLGWLATEKFRDGHATSLGAASGVVAGLVA
HHCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCHHHHHHHHHHH
ITPAAGALTPVTSLILGAIGGILACLGVGLKYRFGFDDSLDVVGVHLVAGLWGTVGVGLL
HCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
ATDIGWFSGGGMDGLKLFIVQIVIALVAVVFAGVITALIAFALKATVGWRVDDDVEQQGI
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCC
DTHEHAESAYDTTGPEIR
CHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
GADQIAAVSGNSAWMLMSASLVLLMTPALALFYGGMSRQKSVLNMMMMSFGALGVVTVI
CCCCEEEECCCCEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHH
YLLWGWSMSYGTQSIAGIFANPFEFFGLKDSIVDADGNYIEGAAGYPNIIDIGFQLTFAV
HHHHCCCHHCCCHHHHHHHCCCHHHCCCCHHHCCCCCCEECCCCCCCCEEHHHHHHHHHH
ISTALISGALAERVKFSTWLIFSGAWVTLVYFPLAHMVWGGGLLGHNVTGFASWLFGSTD
HHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCC
GEANIAPIDFAGGTVVHISAGTAALVLAFIVGKRKTFGKAIARPHNLPMVMLGAALLWFG
CCCCEEEEEECCCEEEEEECCHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHH
WFGFNGGSAFAADGLAGLAWVNTTAATAAAMLGWLATEKFRDGHATSLGAASGVVAGLVA
HHCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCHHHHHHHHHHH
ITPAAGALTPVTSLILGAIGGILACLGVGLKYRFGFDDSLDVVGVHLVAGLWGTVGVGLL
HCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
ATDIGWFSGGGMDGLKLFIVQIVIALVAVVFAGVITALIAFALKATVGWRVDDDVEQQGI
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCC
DTHEHAESAYDTTGPEIR
CHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]