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The map label for this gene is miaB [H]

Identifier: 62390789

GI number: 62390789

Start: 2027901

End: 2029481

Strand: Reverse

Name: miaB [H]

Synonym: cg2135

Alternate gene names: 62390789

Gene position: 2029481-2027901 (Counterclockwise)

Preceding gene: 62390794

Following gene: 62390788

Centisome position: 61.82

GC content: 55.28

Gene sequence:

>1581_bases
GTGACGCAGCAATTGAACCACGCAAAGGTAAATCAACATCCCGGTCAAGCCACCCTCCCAGAAACCGCAGAGGGGCAGGT
TCGCACCTACGAGGTAAAAACCTACGGCTGTCAGATGAATGTGCACGATTCTGAGCGCCTTTCGGGCCTGCTCGAGGAGG
CTGGATACGTTGCTGCTCCGGAGGACACCACTCCGGATCTTGTCGTATTTAATACGTGCGCCGTGCGTGAAAACGCCGAT
ATGCGCCTCTATGGCACTTTGGGCAACCTGCGCAGCGTGAAAGAAAAGAACCCAGGCATGCAAATCGCTGTCGGTGGTTG
TTTGGCTCAAAAAGACAAAGATACCGTGGTGAAAAAAGCACCGTGGGTGGACGTGGTGTTTGGTACCCACAACATTGGTT
CCTTGCCAACCTTGCTTCAGCGCGCGGAGCACAATGCCCAAGCGGAAGTCGAAATTGTCGATTCCCTCGAGCAGTTCCCG
TCAGTACTTTCTGCAAAGCGCGAGTCTGCTTACGCTGGTTGGGTGTCCGTATCAGTCGGATGTAACAACACCTGTACTTT
CTGCATCGTTCCGTCGCTGCGCGGTAAAGAGCAGGACCGTCGACCAGGAGACATCCTCGCAGAGGTACAAGCACTGGTGG
ATCAGGGAGTTACCGAGGTAACTCTACTTGGCCAAAACGTAAATGCTTACGGCGTGAACTTTGTTGATCCTGAGCTAGAG
CGCGATCGCAGTGCATTTTCCAAGCTGCTTCGTGCCTGTGGTGAGATCGAAGGCCTCGAGCGGGTTCGCTTCACCAGCCC
TCACCCTGCAGAATTCACCTCTGATGTCATTGACGCCATGGCAGAGACCCCAAACATCTGCCCGCAGCTGCACATGCCAC
TGCAGTCCGGATCTGACAAGGTGCTCAAAGAGATGCGCCGTTCCTACCGATCCAAGAAGTTCCTCTCCATCTTGGATGAG
GTCCGTGCGAAGATCCCTCACGCCTCTATCACCACCGATATTATTGTCGGATTCCCTGGCGAAACAGAGGAGGATTTCCA
AGCAACCCTCGACGTTGTCAAGAAGGCACGCTTTACTTCTGCTTACACCTTCCAATACAGCCCACGCCCTGGCACCCCTG
CAGCGGAATATGAAAACCAGCTTCCAAAAGAAGTTGTGCAGGAACGCTACGAGCGCCTCATGGTCGTTCAGGAACAAGTC
TGCGAAGAAGAAAACCAAAAGCTCATCGGCACCACCGTCGAATTGCTGGTCCAGGCTGGCGGAGGCCGCAAGAACGATGC
CACCAAGCGCATGAGTGGTCGCGCACGCGATGGACGCCTCGTGCACTTTGCGCCAGAGGGCGACATTGATGGTGAGATCC
GCCCCGGCGATTTTGTCACTGTCACGGTGACTGAGGCCAAGCCTTTCTTCCTCATCGCAGACTCCGGTGTGCAGACCCAC
CGCCGCACCAAAGCTGGTGACAACTCTGCAGTTGGTCAAGTTCCAACCACAGCACCGATCGGTGTGGGCTTGGGACTGCC
ACAAATCGGCGCACCAAAGGTGGCTCCTGCCACAGAATCTGCCTGCTGCTCCATTAACTAA

Upstream 100 bases:

>100_bases
CTAGGAATTCTCAAGGCAAGGTCGGAGACGAGCCTTGTTAGTTCAGAGTCCTAAATAGGCGTTTATGTCAGCTCGCGCAA
CAGGGATACAATGAGCAACC

Downstream 100 bases:

>100_bases
AAATTGCAGGCTAGAATAGAGGGTCGATTAGGAGTCGATGAAAAAGTCGGCGCCAACGAGGAGATGAACAACAACGTGAG
TGATCAAAAGCTAAGTGGTA

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MTQQLNHAKVNQHPGQATLPETAEGQVRTYEVKTYGCQMNVHDSERLSGLLEEAGYVAAPEDTTPDLVVFNTCAVRENAD
MRLYGTLGNLRSVKEKNPGMQIAVGGCLAQKDKDTVVKKAPWVDVVFGTHNIGSLPTLLQRAEHNAQAEVEIVDSLEQFP
SVLSAKRESAYAGWVSVSVGCNNTCTFCIVPSLRGKEQDRRPGDILAEVQALVDQGVTEVTLLGQNVNAYGVNFVDPELE
RDRSAFSKLLRACGEIEGLERVRFTSPHPAEFTSDVIDAMAETPNICPQLHMPLQSGSDKVLKEMRRSYRSKKFLSILDE
VRAKIPHASITTDIIVGFPGETEEDFQATLDVVKKARFTSAYTFQYSPRPGTPAAEYENQLPKEVVQERYERLMVVQEQV
CEEENQKLIGTTVELLVQAGGGRKNDATKRMSGRARDGRLVHFAPEGDIDGEIRPGDFVTVTVTEAKPFFLIADSGVQTH
RRTKAGDNSAVGQVPTTAPIGVGLGLPQIGAPKVAPATESACCSIN

Sequences:

>Translated_526_residues
MTQQLNHAKVNQHPGQATLPETAEGQVRTYEVKTYGCQMNVHDSERLSGLLEEAGYVAAPEDTTPDLVVFNTCAVRENAD
MRLYGTLGNLRSVKEKNPGMQIAVGGCLAQKDKDTVVKKAPWVDVVFGTHNIGSLPTLLQRAEHNAQAEVEIVDSLEQFP
SVLSAKRESAYAGWVSVSVGCNNTCTFCIVPSLRGKEQDRRPGDILAEVQALVDQGVTEVTLLGQNVNAYGVNFVDPELE
RDRSAFSKLLRACGEIEGLERVRFTSPHPAEFTSDVIDAMAETPNICPQLHMPLQSGSDKVLKEMRRSYRSKKFLSILDE
VRAKIPHASITTDIIVGFPGETEEDFQATLDVVKKARFTSAYTFQYSPRPGTPAAEYENQLPKEVVQERYERLMVVQEQV
CEEENQKLIGTTVELLVQAGGGRKNDATKRMSGRARDGRLVHFAPEGDIDGEIRPGDFVTVTVTEAKPFFLIADSGVQTH
RRTKAGDNSAVGQVPTTAPIGVGLGLPQIGAPKVAPATESACCSIN
>Mature_525_residues
TQQLNHAKVNQHPGQATLPETAEGQVRTYEVKTYGCQMNVHDSERLSGLLEEAGYVAAPEDTTPDLVVFNTCAVRENADM
RLYGTLGNLRSVKEKNPGMQIAVGGCLAQKDKDTVVKKAPWVDVVFGTHNIGSLPTLLQRAEHNAQAEVEIVDSLEQFPS
VLSAKRESAYAGWVSVSVGCNNTCTFCIVPSLRGKEQDRRPGDILAEVQALVDQGVTEVTLLGQNVNAYGVNFVDPELER
DRSAFSKLLRACGEIEGLERVRFTSPHPAEFTSDVIDAMAETPNICPQLHMPLQSGSDKVLKEMRRSYRSKKFLSILDEV
RAKIPHASITTDIIVGFPGETEEDFQATLDVVKKARFTSAYTFQYSPRPGTPAAEYENQLPKEVVQERYERLMVVQEQVC
EEENQKLIGTTVELLVQAGGGRKNDATKRMSGRARDGRLVHFAPEGDIDGEIRPGDFVTVTVTEAKPFFLIADSGVQTHR
RTKAGDNSAVGQVPTTAPIGVGLGLPQIGAPKVAPATESACCSIN

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872782, Length=473, Percent_Identity=33.8266384778013, Blast_Score=274, Evalue=2e-73,
Organism=Homo sapiens, GI28872784, Length=473, Percent_Identity=33.8266384778013, Blast_Score=273, Evalue=2e-73,
Organism=Homo sapiens, GI93277076, Length=408, Percent_Identity=28.921568627451, Blast_Score=154, Evalue=3e-37,
Organism=Escherichia coli, GI1786882, Length=447, Percent_Identity=37.3601789709172, Blast_Score=316, Evalue=3e-87,
Organism=Escherichia coli, GI1787057, Length=455, Percent_Identity=31.8681318681319, Blast_Score=201, Evalue=6e-53,
Organism=Caenorhabditis elegans, GI17553146, Length=481, Percent_Identity=32.4324324324324, Blast_Score=247, Evalue=9e-66,
Organism=Caenorhabditis elegans, GI71996771, Length=207, Percent_Identity=34.7826086956522, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI21356207, Length=437, Percent_Identity=34.7826086956522, Blast_Score=279, Evalue=3e-75,
Organism=Drosophila melanogaster, GI19922432, Length=377, Percent_Identity=30.2387267904509, Blast_Score=158, Evalue=8e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 57411; Mature: 57280

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQQLNHAKVNQHPGQATLPETAEGQVRTYEVKTYGCQMNVHDSERLSGLLEEAGYVAAP
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEEECCCCHHHHHHHHHHCCCEECC
EDTTPDLVVFNTCAVRENADMRLYGTLGNLRSVKEKNPGMQIAVGGCLAQKDKDTVVKKA
CCCCCCEEEEEEEEECCCCCEEEEEEHHHHHHHHHCCCCCEEEECCHHCCCCCHHHHHCC
PWVDVVFGTHNIGSLPTLLQRAEHNAQAEVEIVDSLEQFPSVLSAKRESAYAGWVSVSVG
CCEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEECEEEEEEC
CNNTCTFCIVPSLRGKEQDRRPGDILAEVQALVDQGVTEVTLLGQNVNAYGVNFVDPELE
CCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCEEECCCCCCHHH
RDRSAFSKLLRACGEIEGLERVRFTSPHPAEFTSDVIDAMAETPNICPQLHMPLQSGSDK
HHHHHHHHHHHHHHCCCCHHHEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHH
VLKEMRRSYRSKKFLSILDEVRAKIPHASITTDIIVGFPGETEEDFQATLDVVKKARFTS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCE
AYTFQYSPRPGTPAAEYENQLPKEVVQERYERLMVVQEQVCEEENQKLIGTTVELLVQAG
EEEEEECCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
GGRKNDATKRMSGRARDGRLVHFAPEGDIDGEIRPGDFVTVTVTEAKPFFLIADSGVQTH
CCCCCCHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEEECCCCEEEEECCCCCHH
RRTKAGDNSAVGQVPTTAPIGVGLGLPQIGAPKVAPATESACCSIN
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TQQLNHAKVNQHPGQATLPETAEGQVRTYEVKTYGCQMNVHDSERLSGLLEEAGYVAAP
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEEECCCCHHHHHHHHHHCCCEECC
EDTTPDLVVFNTCAVRENADMRLYGTLGNLRSVKEKNPGMQIAVGGCLAQKDKDTVVKKA
CCCCCCEEEEEEEEECCCCCEEEEEEHHHHHHHHHCCCCCEEEECCHHCCCCCHHHHHCC
PWVDVVFGTHNIGSLPTLLQRAEHNAQAEVEIVDSLEQFPSVLSAKRESAYAGWVSVSVG
CCEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEECEEEEEEC
CNNTCTFCIVPSLRGKEQDRRPGDILAEVQALVDQGVTEVTLLGQNVNAYGVNFVDPELE
CCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCEEECCCCCCHHH
RDRSAFSKLLRACGEIEGLERVRFTSPHPAEFTSDVIDAMAETPNICPQLHMPLQSGSDK
HHHHHHHHHHHHHHCCCCHHHEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHH
VLKEMRRSYRSKKFLSILDEVRAKIPHASITTDIIVGFPGETEEDFQATLDVVKKARFTS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCE
AYTFQYSPRPGTPAAEYENQLPKEVVQERYERLMVVQEQVCEEENQKLIGTTVELLVQAG
EEEEEECCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
GGRKNDATKRMSGRARDGRLVHFAPEGDIDGEIRPGDFVTVTVTEAKPFFLIADSGVQTH
CCCCCCHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEEECCCCEEEEECCCCCHH
RRTKAGDNSAVGQVPTTAPIGVGLGLPQIGAPKVAPATESACCSIN
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA