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The map label for this gene is pifA [H]

Identifier: 62390609

GI number: 62390609

Start: 1821650

End: 1823863

Strand: Reverse

Name: pifA [H]

Synonym: cg1945

Alternate gene names: 62390609

Gene position: 1823863-1821650 (Counterclockwise)

Preceding gene: 62390613

Following gene: 62390608

Centisome position: 55.56

GC content: 44.76

Gene sequence:

>2214_bases
GTGCTAGAAATGAGCGGACTGTTTACCCCATTTTCAGATGCGGCAAAAAACAACACGGTAAAAACTGATGGAGATTCAGT
ATCTGGTCGAGACTTGCCTATTACTAAGATCTCTGAGGATCGTTTCGAGCGTTCTGCGTATTCAGCCCAGCTGGCAAATA
TAATCTGCGATGTGGCACCTTGGGGAGCGAGCACTGTTTTCAGTCTTACTGGTCAGTGGGGCAGTGGTAAGACATCTCTT
GTTAATTTGATTCGCTCGGAAGAATCTCTATCGAACGAAAAATGGACAATCGTTGATTTCAACCCGTGGGTGGCCTCTGA
CCCGCAATCTTTGATTGAGGAGTTTTACCGAGTAATCGTTGGGACGGTACCTGATGATAAGACCGGCCAAAAGATCAAAA
CTGTTCTGCAGAAAACCTTTAGCACGATTGGGTCAATTGCAGGTGGGGTCGGAGGGTTTGGTGTCCTAGAAGCACTTGCG
CTCTCAAAAGGAGTAGATGCTGCAAACGCTGTATATAAGACATGGAAACAGGAGCAAGATTCGTGGCCAACGCTGTATAC
ACGTGCTGCGAACCATTTTAAAGATCTGAACAAGCGAATTCTCATTGTCGTCGATGATATTGATCGCCTCCATACTGATG
AATTGGCGCTGTTAATGAAAGTAATACGCTTGCTTGGACGATTCCCGCAGGTGAATTATCTTTTGGTTTATGAAGAAGAA
TCACTGTTAACGACGCTAGCCAGATCGACAGCTGTAGGTGGTAGCGAAGATGATGCTTTGCGTTTCATGGAGAAAATCGT
GCAGTATCCTTTCGATGTTCCGCCTCTGACATCATTTCAAATAGAGAAAGAGCTCAGTGCATTATTTGACAAGCTTTTCC
AGGGTGTTTCGCTATCGGGTGATCCTGAAGACTTTGCACTAGTGAAGTCGAGAATGTTCGATGTCTGGGAAAAGACTCTG
GTCACGCCGAGGCTGTTGCACCGTTTTGCTGCTCTACTAACCAACTGGACTCGGATATATGGATCAGGTGAAGTTAACGG
CGTTGATCTCACAATACTTGCGACCATTCGAATTGTTTTTCCGTCTGTGTATAAACGTCTTTCTCGAGCGAAGGAAGTAT
TGCTTCAAGGAGGTCGAACGACAGGCTCGCAGAAACCCGGTTGGGAAAAGCAATTATGTGAGGGGATGAACAACGAGCAG
ATGGATCTTTTAAAGACCATGCTTTTGTTCCTTTTCCCACGTCTTTCGGATCACCCTAGTACGAGAATGCATCGTGAGAG
GGGGATCTCGACGGAAGTTTATTTTGACACGTACCTCATGTTTCAAAGACCTGGACATGTCATAAGTGATGAACAGTTGG
ATAAGTATCTATCTAATGCGGACGATGCTATGGGTTTCGTCGATTTAATTAACTCCGATGACAATGACATGGTGGCATCA
GTGATGAAAAAGCTTCCTCTAGCAATTGATCGACTTGATGGAGAGGGTGTTAGGCACATGGCAGTTGAGGTGTTATTCAC
CGCTGCTAATGGTATGCATGATAAAGGTCGTCAAGTGCGTATGAGCGGCATATTCAGTGACCTGTATTCCCATGCGTGCT
CGATTCTTGGTGCATTGCCTCAATTACCAGTGGAACAACTCTATGAGAAATTCTTTTCTGAGATGACGCTTAATGAGGCT
GCTTTCTGGTTAAACCAGGTGGGGGAAAGGGCTAGAGCCTGTGGTAATGATGTAAGTGGCCTTGAGCTTTTTCGTAAAGT
TAATATAAAGACCGAAGCTAGAATTTTAAGTGTATTGAAGAATCAGGACCCCTCAGATTGGGATTTAGGTCCATATTCGC
TTGGTATTTTGGCGAAAAGCTCGAATTTTTCTTCAGTGCTGAAGTCTCTGCAAAGTGGTATAGAGGAACATCAGTTTGAT
GTGATAGATATTGGAGTGCTTTTCTTAACGACTGTGTATTCTTCGCGACAGGGACCAAGCGGTGGTGCATGGATAGATTC
TTTTCAGCATAGTCTGTTTTCACGGTACGTACCTGATTCTCTACGGGCTATAACCAAGTCTGAAGTAGATGTAGAACTAG
GTAAGATACAGTTCACGGATTTTAGCTGGGAAGGGAAGCGAAAAGTTGTCGCATATGCACTGGAGACTGGAAGAAGTGAT
TTCACTCGAGAACGATTAGGGGGCTACAGTATCGCAGATTCTATAGTCGATTGA

Upstream 100 bases:

>100_bases
GGCGTAGTAGGCCTTATTAACATACGGCGGTTCTAGCACACAGCGATGGATGATGTGTCCCACCGATATTGCTGCATGTG
CCAAGATTTAGCACGGTACA

Downstream 100 bases:

>100_bases
TGAGGCTGAGGTCATGACTTCAATTAATTCTGGCATAGCGTGTAAGGAGTCAGGTGAATAAATTTCACCATCAGCGGCGA
CTGCATTAAATGATAGAGGA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 737; Mature: 737

Protein sequence:

>737_residues
MLEMSGLFTPFSDAAKNNTVKTDGDSVSGRDLPITKISEDRFERSAYSAQLANIICDVAPWGASTVFSLTGQWGSGKTSL
VNLIRSEESLSNEKWTIVDFNPWVASDPQSLIEEFYRVIVGTVPDDKTGQKIKTVLQKTFSTIGSIAGGVGGFGVLEALA
LSKGVDAANAVYKTWKQEQDSWPTLYTRAANHFKDLNKRILIVVDDIDRLHTDELALLMKVIRLLGRFPQVNYLLVYEEE
SLLTTLARSTAVGGSEDDALRFMEKIVQYPFDVPPLTSFQIEKELSALFDKLFQGVSLSGDPEDFALVKSRMFDVWEKTL
VTPRLLHRFAALLTNWTRIYGSGEVNGVDLTILATIRIVFPSVYKRLSRAKEVLLQGGRTTGSQKPGWEKQLCEGMNNEQ
MDLLKTMLLFLFPRLSDHPSTRMHRERGISTEVYFDTYLMFQRPGHVISDEQLDKYLSNADDAMGFVDLINSDDNDMVAS
VMKKLPLAIDRLDGEGVRHMAVEVLFTAANGMHDKGRQVRMSGIFSDLYSHACSILGALPQLPVEQLYEKFFSEMTLNEA
AFWLNQVGERARACGNDVSGLELFRKVNIKTEARILSVLKNQDPSDWDLGPYSLGILAKSSNFSSVLKSLQSGIEEHQFD
VIDIGVLFLTTVYSSRQGPSGGAWIDSFQHSLFSRYVPDSLRAITKSEVDVELGKIQFTDFSWEGKRKVVAYALETGRSD
FTRERLGGYSIADSIVD

Sequences:

>Translated_737_residues
MLEMSGLFTPFSDAAKNNTVKTDGDSVSGRDLPITKISEDRFERSAYSAQLANIICDVAPWGASTVFSLTGQWGSGKTSL
VNLIRSEESLSNEKWTIVDFNPWVASDPQSLIEEFYRVIVGTVPDDKTGQKIKTVLQKTFSTIGSIAGGVGGFGVLEALA
LSKGVDAANAVYKTWKQEQDSWPTLYTRAANHFKDLNKRILIVVDDIDRLHTDELALLMKVIRLLGRFPQVNYLLVYEEE
SLLTTLARSTAVGGSEDDALRFMEKIVQYPFDVPPLTSFQIEKELSALFDKLFQGVSLSGDPEDFALVKSRMFDVWEKTL
VTPRLLHRFAALLTNWTRIYGSGEVNGVDLTILATIRIVFPSVYKRLSRAKEVLLQGGRTTGSQKPGWEKQLCEGMNNEQ
MDLLKTMLLFLFPRLSDHPSTRMHRERGISTEVYFDTYLMFQRPGHVISDEQLDKYLSNADDAMGFVDLINSDDNDMVAS
VMKKLPLAIDRLDGEGVRHMAVEVLFTAANGMHDKGRQVRMSGIFSDLYSHACSILGALPQLPVEQLYEKFFSEMTLNEA
AFWLNQVGERARACGNDVSGLELFRKVNIKTEARILSVLKNQDPSDWDLGPYSLGILAKSSNFSSVLKSLQSGIEEHQFD
VIDIGVLFLTTVYSSRQGPSGGAWIDSFQHSLFSRYVPDSLRAITKSEVDVELGKIQFTDFSWEGKRKVVAYALETGRSD
FTRERLGGYSIADSIVD
>Mature_737_residues
MLEMSGLFTPFSDAAKNNTVKTDGDSVSGRDLPITKISEDRFERSAYSAQLANIICDVAPWGASTVFSLTGQWGSGKTSL
VNLIRSEESLSNEKWTIVDFNPWVASDPQSLIEEFYRVIVGTVPDDKTGQKIKTVLQKTFSTIGSIAGGVGGFGVLEALA
LSKGVDAANAVYKTWKQEQDSWPTLYTRAANHFKDLNKRILIVVDDIDRLHTDELALLMKVIRLLGRFPQVNYLLVYEEE
SLLTTLARSTAVGGSEDDALRFMEKIVQYPFDVPPLTSFQIEKELSALFDKLFQGVSLSGDPEDFALVKSRMFDVWEKTL
VTPRLLHRFAALLTNWTRIYGSGEVNGVDLTILATIRIVFPSVYKRLSRAKEVLLQGGRTTGSQKPGWEKQLCEGMNNEQ
MDLLKTMLLFLFPRLSDHPSTRMHRERGISTEVYFDTYLMFQRPGHVISDEQLDKYLSNADDAMGFVDLINSDDNDMVAS
VMKKLPLAIDRLDGEGVRHMAVEVLFTAANGMHDKGRQVRMSGIFSDLYSHACSILGALPQLPVEQLYEKFFSEMTLNEA
AFWLNQVGERARACGNDVSGLELFRKVNIKTEARILSVLKNQDPSDWDLGPYSLGILAKSSNFSSVLKSLQSGIEEHQFD
VIDIGVLFLTTVYSSRQGPSGGAWIDSFQHSLFSRYVPDSLRAITKSEVDVELGKIQFTDFSWEGKRKVVAYALETGRSD
FTRERLGGYSIADSIVD

Specific function: Responsible for the exclusion of phage T7 by plasmid F. Growth of bacteriophage T7 is inhibited in cells of E.coli that carries the plasmid F [H]

COG id: COG4928

COG function: function code R; Predicted P-loop ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 KAP NTPase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011646 [H]

Pfam domain/function: PF07693 KAP_NTPase [H]

EC number: NA

Molecular weight: Translated: 82355; Mature: 82355

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEMSGLFTPFSDAAKNNTVKTDGDSVSGRDLPITKISEDRFERSAYSAQLANIICDVAP
CCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
WGASTVFSLTGQWGSGKTSLVNLIRSEESLSNEKWTIVDFNPWVASDPQSLIEEFYRVIV
CCCCHHEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHH
GTVPDDKTGQKIKTVLQKTFSTIGSIAGGVGGFGVLEALALSKGVDAANAVYKTWKQEQD
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
SWPTLYTRAANHFKDLNKRILIVVDDIDRLHTDELALLMKVIRLLGRFPQVNYLLVYEEE
CCCHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCH
SLLTTLARSTAVGGSEDDALRFMEKIVQYPFDVPPLTSFQIEKELSALFDKLFQGVSLSG
HHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
DPEDFALVKSRMFDVWEKTLVTPRLLHRFAALLTNWTRIYGSGEVNGVDLTILATIRIVF
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHH
PSVYKRLSRAKEVLLQGGRTTGSQKPGWEKQLCEGMNNEQMDLLKTMLLFLFPRLSDHPS
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCC
TRMHRERGISTEVYFDTYLMFQRPGHVISDEQLDKYLSNADDAMGFVDLINSDDNDMVAS
HHHHHHHCCCEEEEEHHHHEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHH
VMKKLPLAIDRLDGEGVRHMAVEVLFTAANGMHDKGRQVRMSGIFSDLYSHACSILGALP
HHHHCCHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
QLPVEQLYEKFFSEMTLNEAAFWLNQVGERARACGNDVSGLELFRKVNIKTEARILSVLK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHH
NQDPSDWDLGPYSLGILAKSSNFSSVLKSLQSGIEEHQFDVIDIGVLFLTTVYSSRQGPS
CCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCC
GGAWIDSFQHSLFSRYVPDSLRAITKSEVDVELGKIQFTDFSWEGKRKVVAYALETGRSD
CCHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEECEEEEECCCCCCCHHHEEHHHHCCCCH
FTRERLGGYSIADSIVD
HHHHHHCCHHHHHHHCC
>Mature Secondary Structure
MLEMSGLFTPFSDAAKNNTVKTDGDSVSGRDLPITKISEDRFERSAYSAQLANIICDVAP
CCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
WGASTVFSLTGQWGSGKTSLVNLIRSEESLSNEKWTIVDFNPWVASDPQSLIEEFYRVIV
CCCCHHEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHH
GTVPDDKTGQKIKTVLQKTFSTIGSIAGGVGGFGVLEALALSKGVDAANAVYKTWKQEQD
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
SWPTLYTRAANHFKDLNKRILIVVDDIDRLHTDELALLMKVIRLLGRFPQVNYLLVYEEE
CCCHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCH
SLLTTLARSTAVGGSEDDALRFMEKIVQYPFDVPPLTSFQIEKELSALFDKLFQGVSLSG
HHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
DPEDFALVKSRMFDVWEKTLVTPRLLHRFAALLTNWTRIYGSGEVNGVDLTILATIRIVF
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHH
PSVYKRLSRAKEVLLQGGRTTGSQKPGWEKQLCEGMNNEQMDLLKTMLLFLFPRLSDHPS
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCC
TRMHRERGISTEVYFDTYLMFQRPGHVISDEQLDKYLSNADDAMGFVDLINSDDNDMVAS
HHHHHHHCCCEEEEEHHHHEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHH
VMKKLPLAIDRLDGEGVRHMAVEVLFTAANGMHDKGRQVRMSGIFSDLYSHACSILGALP
HHHHCCHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
QLPVEQLYEKFFSEMTLNEAAFWLNQVGERARACGNDVSGLELFRKVNIKTEARILSVLK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHH
NQDPSDWDLGPYSLGILAKSSNFSSVLKSLQSGIEEHQFDVIDIGVLFLTTVYSSRQGPS
CCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCC
GGAWIDSFQHSLFSRYVPDSLRAITKSEVDVELGKIQFTDFSWEGKRKVVAYALETGRSD
CCHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEECEEEEECCCCCCCHHHEEHHHHCCCCH
FTRERLGGYSIADSIVD
HHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 5283385 [H]