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The map label for this gene is nifS2 [H]

Identifier: 62390444

GI number: 62390444

Start: 1649564

End: 1650829

Strand: Reverse

Name: nifS2 [H]

Synonym: cg1761

Alternate gene names: 62390444

Gene position: 1650829-1649564 (Counterclockwise)

Preceding gene: 62390445

Following gene: 62390443

Centisome position: 50.29

GC content: 54.82

Gene sequence:

>1266_bases
ATGTCCGATTTCCTCAATGCAGATGGATCCCTCAATGTGGATAAGGTGCGGGAAGAATTCCCAATCCTGAAGCGCACTGT
TAGGGATGGGAAACCGCTTGCTTACCTGGACTCAGGTGCGACATCGCAGCGACCCGAGCGGGTGTGGCGTGCAGAGGAGC
ACTTTGTGCTGCACACCAACGCCCCCGTGCACCGCGGTGCCTACCAACTGGCTGAGGAAGCAACGGATGCTTATGAAGGT
GCCCGCGAGAAGATCGCTGCCTTTGTTGGTGCCGAGCAGCATGAAATTGCGTTCACTAAGAATGCAACTGAAGCACTCAA
TCTTGTTGCGTACACCTTGGGTGATGACCGTTCCGGTAAGTATCGTGTCCAGGCCGGGGATACCGTGGTCATCACGGAGC
TAGAGCACCACGCAAACTTGGTGCCATGGCAGGAGCTGTGCCGTCGAACCGGTGCGACATTGAAGTGGTACAAGGTGACT
GAAGATGGTCGCATTGATCTCGATTCACTCGAGCTTGATGAAACTGTCAAGGTCGTTGCCTTCACTCACCAGTCCAATGT
GACCGGTGCTGTGGCTGATGTTCCAGAGTTGGTTCGTCGTGCCAAGGCTGTCGGCGCTCTCACGGTGCTTGATGCGTGCC
AGTCTGTTCCTCATATGCCAGTGAATTTCCACGAGCTGGATGTAGATTTCTCTGCATTCTCTGGCCATAAGATGCTGGGA
CCTGCAGGCGTGGGCGTTGTGTATGCAAAGTCCCCAATCTTGGATGAACTGCCACCATTTTTGACTGGTGGTTCCATGAT
TGAAGTTGTCACCATGGAGGGTTCCACCTACGCTGCCGCACCTCAACGTTTTGAGGCCGGCACGCAGATGACCAGCCAGG
TTGTGGGCTTGGGTGCTGCCGTGGACATGCTGAATGAAATCGGTATGGAAGCAATCGCAGCGCATGAGCACGCATTGACT
GCTTACGCGTTGGAAAAGCTCACGGCAATTAAGGGACTAACCATTGCTGGTCCTTTGACTGCAGAGCAGCGCGGCGGTGC
AATCAGCTTCGGTGTCGAGGGCATTCACCCACACGATCTAGGCCAAGTGCTTGACGATCAGGGCGTGAATATCCGCGTCG
GCCACCACTGCGCGTGGCCCGTGCACCGCAGCATGAACGTACAATCGACAGCAAGAGCATCTTTCTATCTCTATAACACC
TTCGAAGAAATCGACCGCCTCGCGGCAGCGATCGAGAAGGCCAAGCAATTCTTTGGAGTTGAGTAA

Upstream 100 bases:

>100_bases
TTCATTCACGTTTTCGCGAATGGCCAGATTGTGACCACCGGTGGCGCTGAGCTTGCTGACAAGCTCGAGGCTGACGGCTA
CGACCAGTTCATCAAGTAAC

Downstream 100 bases:

>100_bases
TGAACCTTGAGCAGATGTACCAGGAGGTGATCCTGGACCACTACAAAAACCCACAGCACAAGGGCCTTCGGGATCCTTTC
GATGCTGAGGTTCACCACGT

Product: cysteine desulfhydrase / selenocysteine lyase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MSDFLNADGSLNVDKVREEFPILKRTVRDGKPLAYLDSGATSQRPERVWRAEEHFVLHTNAPVHRGAYQLAEEATDAYEG
AREKIAAFVGAEQHEIAFTKNATEALNLVAYTLGDDRSGKYRVQAGDTVVITELEHHANLVPWQELCRRTGATLKWYKVT
EDGRIDLDSLELDETVKVVAFTHQSNVTGAVADVPELVRRAKAVGALTVLDACQSVPHMPVNFHELDVDFSAFSGHKMLG
PAGVGVVYAKSPILDELPPFLTGGSMIEVVTMEGSTYAAAPQRFEAGTQMTSQVVGLGAAVDMLNEIGMEAIAAHEHALT
AYALEKLTAIKGLTIAGPLTAEQRGGAISFGVEGIHPHDLGQVLDDQGVNIRVGHHCAWPVHRSMNVQSTARASFYLYNT
FEEIDRLAAAIEKAKQFFGVE

Sequences:

>Translated_421_residues
MSDFLNADGSLNVDKVREEFPILKRTVRDGKPLAYLDSGATSQRPERVWRAEEHFVLHTNAPVHRGAYQLAEEATDAYEG
AREKIAAFVGAEQHEIAFTKNATEALNLVAYTLGDDRSGKYRVQAGDTVVITELEHHANLVPWQELCRRTGATLKWYKVT
EDGRIDLDSLELDETVKVVAFTHQSNVTGAVADVPELVRRAKAVGALTVLDACQSVPHMPVNFHELDVDFSAFSGHKMLG
PAGVGVVYAKSPILDELPPFLTGGSMIEVVTMEGSTYAAAPQRFEAGTQMTSQVVGLGAAVDMLNEIGMEAIAAHEHALT
AYALEKLTAIKGLTIAGPLTAEQRGGAISFGVEGIHPHDLGQVLDDQGVNIRVGHHCAWPVHRSMNVQSTARASFYLYNT
FEEIDRLAAAIEKAKQFFGVE
>Mature_420_residues
SDFLNADGSLNVDKVREEFPILKRTVRDGKPLAYLDSGATSQRPERVWRAEEHFVLHTNAPVHRGAYQLAEEATDAYEGA
REKIAAFVGAEQHEIAFTKNATEALNLVAYTLGDDRSGKYRVQAGDTVVITELEHHANLVPWQELCRRTGATLKWYKVTE
DGRIDLDSLELDETVKVVAFTHQSNVTGAVADVPELVRRAKAVGALTVLDACQSVPHMPVNFHELDVDFSAFSGHKMLGP
AGVGVVYAKSPILDELPPFLTGGSMIEVVTMEGSTYAAAPQRFEAGTQMTSQVVGLGAAVDMLNEIGMEAIAAHEHALTA
YALEKLTAIKGLTIAGPLTAEQRGGAISFGVEGIHPHDLGQVLDDQGVNIRVGHHCAWPVHRSMNVQSTARASFYLYNTF
EEIDRLAAAIEKAKQFFGVE

Specific function: Catalyzes the removal of elemental sulfur and selenium atoms from L-cysteine, L-cystine, L-selenocysteine, and L- selenocystine to produce L-alanine [H]

COG id: COG0520

COG function: function code E; Selenocysteine lyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. Csd subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=281, Percent_Identity=25.9786476868327, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI156713448, Length=290, Percent_Identity=26.551724137931, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1787970, Length=413, Percent_Identity=42.6150121065375, Blast_Score=350, Evalue=1e-97,
Organism=Escherichia coli, GI1789175, Length=411, Percent_Identity=33.3333333333333, Blast_Score=238, Evalue=4e-64,
Organism=Escherichia coli, GI48994898, Length=282, Percent_Identity=30.4964539007092, Blast_Score=107, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI193211090, Length=393, Percent_Identity=27.4809160305344, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI25143064, Length=218, Percent_Identity=25.2293577981651, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6319831, Length=239, Percent_Identity=25.9414225941423, Blast_Score=80, Evalue=7e-16,
Organism=Drosophila melanogaster, GI20129463, Length=286, Percent_Identity=27.972027972028, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010970
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 45801; Mature: 45670

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDFLNADGSLNVDKVREEFPILKRTVRDGKPLAYLDSGATSQRPERVWRAEEHFVLHTN
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHCCCCCEEEECC
APVHRGAYQLAEEATDAYEGAREKIAAFVGAEQHEIAFTKNATEALNLVAYTLGDDRSGK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHEEEEEEECCCCCCE
YRVQAGDTVVITELEHHANLVPWQELCRRTGATLKWYKVTEDGRIDLDSLELDETVKVVA
EEEECCCEEEEEEEHHCCCCCCHHHHHHHCCCEEEEEEECCCCCEECCCCCCCCEEEEEE
FTHQSNVTGAVADVPELVRRAKAVGALTVLDACQSVPHMPVNFHELDVDFSAFSGHKMLG
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHCCCCCEEC
PAGVGVVYAKSPILDELPPFLTGGSMIEVVTMEGSTYAAAPQRFEAGTQMTSQVVGLGAA
CCCCEEEEECCCCHHHCCCCCCCCCEEEEEEECCCEEECCCHHHHHHHHHHHHHHHHHHH
VDMLNEIGMEAIAAHEHALTAYALEKLTAIKGLTIAGPLTAEQRGGAISFGVEGIHPHDL
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHCCCEEEECCCCCCHHHH
GQVLDDQGVNIRVGHHCAWPVHRSMNVQSTARASFYLYNTFEEIDRLAAAIEKAKQFFGV
HHHHCCCCCEEEECCEECCCCCCCCCCCHHHCEEEEEEHHHHHHHHHHHHHHHHHHHHCC
E
C
>Mature Secondary Structure 
SDFLNADGSLNVDKVREEFPILKRTVRDGKPLAYLDSGATSQRPERVWRAEEHFVLHTN
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHCCCCCEEEECC
APVHRGAYQLAEEATDAYEGAREKIAAFVGAEQHEIAFTKNATEALNLVAYTLGDDRSGK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHEEEEEEECCCCCCE
YRVQAGDTVVITELEHHANLVPWQELCRRTGATLKWYKVTEDGRIDLDSLELDETVKVVA
EEEECCCEEEEEEEHHCCCCCCHHHHHHHCCCEEEEEEECCCCCEECCCCCCCCEEEEEE
FTHQSNVTGAVADVPELVRRAKAVGALTVLDACQSVPHMPVNFHELDVDFSAFSGHKMLG
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHCCCCCEEC
PAGVGVVYAKSPILDELPPFLTGGSMIEVVTMEGSTYAAAPQRFEAGTQMTSQVVGLGAA
CCCCEEEEECCCCHHHCCCCCCCCCEEEEEEECCCEEECCCHHHHHHHHHHHHHHHHHHH
VDMLNEIGMEAIAAHEHALTAYALEKLTAIKGLTIAGPLTAEQRGGAISFGVEGIHPHDL
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHCCCEEEECCCCCCHHHH
GQVLDDQGVNIRVGHHCAWPVHRSMNVQSTARASFYLYNTFEEIDRLAAAIEKAKQFFGV
HHHHCCCCCEEEECCEECCCCCCCCCCCHHHCEEEEEEHHHHHHHHHHHHHHHHHHHHCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]