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The map label for this gene is argF

Identifier: 62390283

GI number: 62390283

Start: 1470032

End: 1470991

Strand: Direct

Name: argF

Synonym: cg1584

Alternate gene names: 62390283

Gene position: 1470032-1470991 (Clockwise)

Preceding gene: 62390282

Following gene: 62390284

Centisome position: 44.78

GC content: 56.56

Gene sequence:

>960_bases
ATGACTTCACAACCACAGGTTCGCCATTTTCTGGCTGATGATGATCTCACCCCTGCAGAGCAGGCAGAGGTTTTGACCCT
AGCCGCAAAGCTCAAGGCAGCGCCGTTTTCGGAGCGTCCACTCGAGGGACCAAAGTCCGTTGCAGTTCTTTTTGATAAGA
CTTCAACTCGTACTCGCTTCTCCTTCGACGCGGGCATCGCTCATTTGGGTGGACACGCCATCGTCGTGGATTCCGGTAGC
TCACAGATGGGTAAGGGCGAGTCCCTGCAGGACACCGCAGCTGTATTGTCCCGCTACGTGGAAGCAATTGTGTGGCGCAC
CTACGCACACAGCAATTTCCACGCCATGGCGGAGACGTCCACTGTGCCGCTGGTGAACTCCTTGTCCGATGATCTGCACC
CATGCCAGATTCTGGCTGATCTGCAGACTATCGTGGAAAACCTCAGCCCTGAAGAAGGCCCAGCAGGCCTTAAGGGTAAG
AAGGCTGTGTACCTGGGCGATGGCGACAACAACATGGCCAACTCCTACATGATTGGCTTTGCCACCGCGGGCATGGATAT
TTCCATCATCGCTCCTGAAGGGTTCCAGCCTCGTGCGGAATTCGTGGAGCGCGCGGAAAAGCGTGGCCAGGAAACCGGCG
CGAAGGTTGTTGTCACCGACAGCCTCGACGAGGTTGCCGGCGCCGATGTTGTCATCACCGATACCTGGGTATCCATGGGT
ATGGAAAACGACGGCATCGATCGCACCACACCTTTCGTTCCTTACCAGGTCAACGATGAGGTCATGGCGAAAGCTAACGA
CGGCGCCATCTTCCTGCACTGCCTTCCTGCCTACCGTGGCAAAGAAGTGGCAGCCTCCGTGATTGATGGACCAGCGTCCA
AAGTTTTCGATGAAGCAGAAAACCGCCTCCACGCTCAGAAAGCACTGCTGGTGTGGCTGCTGGCCAACCAGCCGAGGTAA

Upstream 100 bases:

>100_bases
AACATTATCCGTTTGACCCCGCCGCTGGTGATCACCGACGAAGAAATCGCAGACGCAGTCAAGGCTATTGCCGAGACAAT
CGCATAAAGGACTCAAACTT

Downstream 100 bases:

>100_bases
GACATGTCCCTTGGCTCAACCCCGTCAACACCGGAAAACTTAAATCCCGTGACTCGCACTGCACGCCAAGCTCTCATTTT
GCAGATTTTGGACAAACAAA

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MTSQPQVRHFLADDDLTPAEQAEVLTLAAKLKAAPFSERPLEGPKSVAVLFDKTSTRTRFSFDAGIAHLGGHAIVVDSGS
SQMGKGESLQDTAAVLSRYVEAIVWRTYAHSNFHAMAETSTVPLVNSLSDDLHPCQILADLQTIVENLSPEEGPAGLKGK
KAVYLGDGDNNMANSYMIGFATAGMDISIIAPEGFQPRAEFVERAEKRGQETGAKVVVTDSLDEVAGADVVITDTWVSMG
MENDGIDRTTPFVPYQVNDEVMAKANDGAIFLHCLPAYRGKEVAASVIDGPASKVFDEAENRLHAQKALLVWLLANQPR

Sequences:

>Translated_319_residues
MTSQPQVRHFLADDDLTPAEQAEVLTLAAKLKAAPFSERPLEGPKSVAVLFDKTSTRTRFSFDAGIAHLGGHAIVVDSGS
SQMGKGESLQDTAAVLSRYVEAIVWRTYAHSNFHAMAETSTVPLVNSLSDDLHPCQILADLQTIVENLSPEEGPAGLKGK
KAVYLGDGDNNMANSYMIGFATAGMDISIIAPEGFQPRAEFVERAEKRGQETGAKVVVTDSLDEVAGADVVITDTWVSMG
MENDGIDRTTPFVPYQVNDEVMAKANDGAIFLHCLPAYRGKEVAASVIDGPASKVFDEAENRLHAQKALLVWLLANQPR
>Mature_318_residues
TSQPQVRHFLADDDLTPAEQAEVLTLAAKLKAAPFSERPLEGPKSVAVLFDKTSTRTRFSFDAGIAHLGGHAIVVDSGSS
QMGKGESLQDTAAVLSRYVEAIVWRTYAHSNFHAMAETSTVPLVNSLSDDLHPCQILADLQTIVENLSPEEGPAGLKGKK
AVYLGDGDNNMANSYMIGFATAGMDISIIAPEGFQPRAEFVERAEKRGQETGAKVVVTDSLDEVAGADVVITDTWVSMGM
ENDGIDRTTPFVPYQVNDEVMAKANDGAIFLHCLPAYRGKEVAASVIDGPASKVFDEAENRLHAQKALLVWLLANQPR

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI38788445, Length=317, Percent_Identity=38.4858044164038, Blast_Score=195, Evalue=4e-50,
Organism=Homo sapiens, GI18105007, Length=318, Percent_Identity=24.2138364779874, Blast_Score=76, Evalue=4e-14,
Organism=Escherichia coli, GI1790703, Length=333, Percent_Identity=37.5375375375375, Blast_Score=201, Evalue=7e-53,
Organism=Escherichia coli, GI1786469, Length=327, Percent_Identity=35.474006116208, Blast_Score=190, Evalue=9e-50,
Organism=Escherichia coli, GI48994908, Length=323, Percent_Identity=27.8637770897833, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI2367364, Length=319, Percent_Identity=29.7805642633229, Blast_Score=80, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6322373, Length=277, Percent_Identity=35.3790613718412, Blast_Score=167, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6322331, Length=311, Percent_Identity=25.7234726688103, Blast_Score=73, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24642586, Length=316, Percent_Identity=27.2151898734177, Blast_Score=89, Evalue=3e-18,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): OTC_CORGL (Q59283)

Other databases:

- EMBL:   AF031518
- EMBL:   AF049897
- EMBL:   BA000036
- EMBL:   BX927152
- EMBL:   X86157
- RefSeq:   NP_600617.1
- RefSeq:   YP_225685.1
- ProteinModelPortal:   Q59283
- SMR:   Q59283
- World-2DPAGE:   0001:Q59283
- PRIDE:   Q59283
- GeneID:   1019374
- GeneID:   3345366
- GenomeReviews:   BA000036_GR
- GenomeReviews:   BX927147_GR
- KEGG:   cgb:cg1584
- KEGG:   cgl:NCgl1344
- HOGENOM:   HBG579429
- OMA:   HATIPVV
- PhylomeDB:   Q59283
- ProtClustDB:   PRK00779
- BioCyc:   CGLU196627:CG1584-MONOMER
- BRENDA:   2.1.3.3
- HAMAP:   MF_01109
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292
- PRINTS:   PR00100
- PRINTS:   PR00102
- TIGRFAMs:   TIGR00658

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.3

Molecular weight: Translated: 34329; Mature: 34198

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: BINDING 106-106 BINDING 133-133

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSQPQVRHFLADDDLTPAEQAEVLTLAAKLKAAPFSERPLEGPKSVAVLFDKTSTRTRF
CCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCEEE
SFDAGIAHLGGHAIVVDSGSSQMGKGESLQDTAAVLSRYVEAIVWRTYAHSNFHAMAETS
EECCCHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
TVPLVNSLSDDLHPCQILADLQTIVENLSPEEGPAGLKGKKAVYLGDGDNNMANSYMIGF
CCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEE
ATAGMDISIIAPEGFQPRAEFVERAEKRGQETGAKVVVTDSLDEVAGADVVITDTWVSMG
EECCCEEEEECCCCCCCHHHHHHHHHHCCHHCCCEEEEECCHHHHCCCCEEEEEHHHHCC
MENDGIDRTTPFVPYQVNDEVMAKANDGAIFLHCLPAYRGKEVAASVIDGPASKVFDEAE
CCCCCCCCCCCCCEEEECCHHEEECCCCEEEEEEECCCCCHHHHHHHHCCCHHHHHHHHH
NRLHAQKALLVWLLANQPR
HHHHHHHHHEEEEECCCCC
>Mature Secondary Structure 
TSQPQVRHFLADDDLTPAEQAEVLTLAAKLKAAPFSERPLEGPKSVAVLFDKTSTRTRF
CCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCEEE
SFDAGIAHLGGHAIVVDSGSSQMGKGESLQDTAAVLSRYVEAIVWRTYAHSNFHAMAETS
EECCCHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
TVPLVNSLSDDLHPCQILADLQTIVENLSPEEGPAGLKGKKAVYLGDGDNNMANSYMIGF
CCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEE
ATAGMDISIIAPEGFQPRAEFVERAEKRGQETGAKVVVTDSLDEVAGADVVITDTWVSMG
EECCCEEEEECCCCCCCHHHHHHHHHHCCHHCCCEEEEECCHHHHCCCCEEEEEHHHHCC
MENDGIDRTTPFVPYQVNDEVMAKANDGAIFLHCLPAYRGKEVAASVIDGPASKVFDEAE
CCCCCCCCCCCCCEEEECCHHEEECCCCEEEEEEECCCCCHHHHHHHHCCCHHHHHHHHH
NRLHAQKALLVWLLANQPR
HHHHHHHHHEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10420995; 12948626; 8581175