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The map label for this gene is tsnR [H]

Identifier: 62390273

GI number: 62390273

Start: 1457570

End: 1458418

Strand: Direct

Name: tsnR [H]

Synonym: cg1573

Alternate gene names: 62390273

Gene position: 1457570-1458418 (Clockwise)

Preceding gene: 62390271

Following gene: 62390274

Centisome position: 44.4

GC content: 56.89

Gene sequence:

>849_bases
TTGACCGCACGGTTAACTGTTAGTGTTATGAGCATGGCATTGGACTTTAATGAGGCGTTTACCGAACGCACCCCGCGCAT
CGTCAACGCAGCTAAACTGCATCGCGCCGCGCAGCGCAAAAAAGATAAGCGTTTTCTAGTTGAAGGCGAAAATTCCGTTG
AAGCAGCTGTCGCTACCGGCGCAGCAACTGACCTCTTTGTCACTGAATCCGCTGCGGAGCGCTTCGAGGAAATCGTCCGC
ACCGCCGGTTACATGAATGTCTACACCCACGCGATCACGGACAAGGCCGCGAAGCATCTTAGCGACACCGTCACCACCAC
GGGCATTTTTGCGCTTTGCGACGACGTCCTGTGGTCAGTCGGCAAGGCGATCACCGGCCAGCCACGTCTAGTGAGCGTGC
CGGTTGAGACCCGCGAGCCCGGTAATGCCGGAACCTTGATTCGCGTATCCGACGCGGTCGGCGCCGACGCCGTCGTCTTC
GCTGGTGAATCAGTAGATCCACTTGGCGCAAAAGCTGTGCGCTCCTCAGCGGGATCGCTGTTTCACATTCCAGTGGCACG
CAACAACAACATCGCAGATGTCTTGGGGCAGCTTCGTTCCAAGGGTCTGCAGATCCTTGCGACCTCAGCCGATGGGGAAG
TAGACCTCGATGACGCCGATGAGCTGCTAGCCAAGCCAACCGCATGGCTTTTTGGTAATGAAGCTCACGGACTTGATGAG
AGCCTGCTTGCTCAGGCTGATCACCGCGTGCGTATTCCGATCCGCGGCCGCGCAGAATCACTCAATTTGGCCACAGCAGC
GTCAATTTGTCTGTACGAATCCTCCAAGGCACTATTCGCCGGTGAGTAA

Upstream 100 bases:

>100_bases
TGGTGTTTTCGAGGTGCAGATCTTCCGCACCGCGATGGGCAACAATGTGCATATTCTTATACATCCTTTTCTGTGGGCTT
TTTAAGGATAGGGAGCAAAG

Downstream 100 bases:

>100_bases
ACCCCAATTCATGCCCAGCGGGTATGATGGCGGGGTTAGTTGACTTAACAATAAACATGAGGGAAGGGCTGGACTACACG
GTGTCCGAAATTCAGTTGAC

Product: 23S ribosomal RNA methyltransferase

Products: S-adenosyl-L-homocysteine; tRNATyr Containing 2'-O-methylguanosine [C]

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MTARLTVSVMSMALDFNEAFTERTPRIVNAAKLHRAAQRKKDKRFLVEGENSVEAAVATGAATDLFVTESAAERFEEIVR
TAGYMNVYTHAITDKAAKHLSDTVTTTGIFALCDDVLWSVGKAITGQPRLVSVPVETREPGNAGTLIRVSDAVGADAVVF
AGESVDPLGAKAVRSSAGSLFHIPVARNNNIADVLGQLRSKGLQILATSADGEVDLDDADELLAKPTAWLFGNEAHGLDE
SLLAQADHRVRIPIRGRAESLNLATAASICLYESSKALFAGE

Sequences:

>Translated_282_residues
MTARLTVSVMSMALDFNEAFTERTPRIVNAAKLHRAAQRKKDKRFLVEGENSVEAAVATGAATDLFVTESAAERFEEIVR
TAGYMNVYTHAITDKAAKHLSDTVTTTGIFALCDDVLWSVGKAITGQPRLVSVPVETREPGNAGTLIRVSDAVGADAVVF
AGESVDPLGAKAVRSSAGSLFHIPVARNNNIADVLGQLRSKGLQILATSADGEVDLDDADELLAKPTAWLFGNEAHGLDE
SLLAQADHRVRIPIRGRAESLNLATAASICLYESSKALFAGE
>Mature_281_residues
TARLTVSVMSMALDFNEAFTERTPRIVNAAKLHRAAQRKKDKRFLVEGENSVEAAVATGAATDLFVTESAAERFEEIVRT
AGYMNVYTHAITDKAAKHLSDTVTTTGIFALCDDVLWSVGKAITGQPRLVSVPVETREPGNAGTLIRVSDAVGADAVVFA
GESVDPLGAKAVRSSAGSLFHIPVARNNNIADVLGQLRSKGLQILATSADGEVDLDDADELLAKPTAWLFGNEAHGLDES
LLAQADHRVRIPIRGRAESLNLATAASICLYESSKALFAGE

Specific function: Specifically Methylates Guanosine-18 In Various tRNAs. [C]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790083, Length=153, Percent_Identity=33.9869281045752, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1790623, Length=138, Percent_Identity=28.2608695652174, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.34 [C]

Molecular weight: Translated: 29990; Mature: 29859

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARLTVSVMSMALDFNEAFTERTPRIVNAAKLHRAAQRKKDKRFLVEGENSVEAAVATG
CCCEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHCC
AATDLFVTESAAERFEEIVRTAGYMNVYTHAITDKAAKHLSDTVTTTGIFALCDDVLWSV
CCCEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GKAITGQPRLVSVPVETREPGNAGTLIRVSDAVGADAVVFAGESVDPLGAKAVRSSAGSL
HHHCCCCCEEEEECCCCCCCCCCCCEEEEECCCCCCEEEEECCCCCCCCHHHHHHCCCCE
FHIPVARNNNIADVLGQLRSKGLQILATSADGEVDLDDADELLAKPTAWLFGNEAHGLDE
EEEEECCCCCHHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHCCCCEEECCCCCCCCH
SLLAQADHRVRIPIRGRAESLNLATAASICLYESSKALFAGE
HHHHCCCCEEEEEECCCCCCCCHHHHHEEEEEECCCCEECCC
>Mature Secondary Structure 
TARLTVSVMSMALDFNEAFTERTPRIVNAAKLHRAAQRKKDKRFLVEGENSVEAAVATG
CCEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHCC
AATDLFVTESAAERFEEIVRTAGYMNVYTHAITDKAAKHLSDTVTTTGIFALCDDVLWSV
CCCEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GKAITGQPRLVSVPVETREPGNAGTLIRVSDAVGADAVVFAGESVDPLGAKAVRSSAGSL
HHHCCCCCEEEEECCCCCCCCCCCCEEEEECCCCCCEEEEECCCCCCCCHHHHHHCCCCE
FHIPVARNNNIADVLGQLRSKGLQILATSADGEVDLDDADELLAKPTAWLFGNEAHGLDE
EEEEECCCCCHHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHCCCCEEECCCCCCCCH
SLLAQADHRVRIPIRGRAESLNLATAASICLYESSKALFAGE
HHHHCCCCEEEEEECCCCCCCCHHHHHEEEEEECCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; tRNATyr [C]

Specific reaction: S-Adenosyl-L-methionine + tRNATyr = S-adenosyl-L-homocysteine + tRNATyr Containing 2'-O-methylguanosine [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]