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The map label for this gene is spoT [H]

Identifier: 62390196

GI number: 62390196

Start: 1380470

End: 1381036

Strand: Direct

Name: spoT [H]

Synonym: cg1485

Alternate gene names: 62390196

Gene position: 1380470-1381036 (Clockwise)

Preceding gene: 62390190

Following gene: 62390198

Centisome position: 42.05

GC content: 51.15

Gene sequence:

>567_bases
GTGAATACACTTTCCCCGCGCCTTCGCAAAGCTATGAATACTGCCGCGTGGGCACACCGCCACCACGTACGCAAAGGCGG
TGGAATTCCGTATGTCAGCCATCTTTATTCAGTGATGTACTTGCTGGCCAGCGTCACTAATGATGAAGATGTGCTCATCG
CCGGGCTGCTCCACGACACCCTCGAAGACGTACCCGAGGAATACAATTCTGCCCAACTTGAAGCTGATTTTGGTCCGCGG
GTGCGCGAGTTGGTGGAAGAGCTCACCAAACAGCCCTTAAAAAGCTGGAAAGCGCGTGCCGACGCTTACCTCCTGCACCT
CAGCGCAGGTGCCAGCTTAGAGGCTGTCTTAATCTCCACCGCAGATAAACTGCATAATCTCATGTCCATCTTGGATGACC
TTGAAATACACGGTGAAGATTTATGGCAACGCTTTAACGCTGGCAAAGAGCAGCAAATCTGGTGGTATAGCGAGGTTTAT
CAGATATCTCTCCAGCGCTTAGGGTTCAATGAGTTGAATAAACAACTGGGGTTGTGCGTCGAAAAGCTCTTAAAGCAAAG
CGCCTAG

Upstream 100 bases:

>100_bases
AAACTGTGAGGATTAAGGCTCTAGACTGGAATTTCAACATCTATTCCTCCTGCGATTTGCATGGGATATATATTAAAAAT
TCTAGCCGAAAGTTTCCTGC

Downstream 100 bases:

>100_bases
GCGCTCGGCGGCGTCGATAAGCTCGGTGCCCCACATGGCGGCGGGGTGGGGCTTGAGGCGCTCGGCCACGCCGGAGATGG
ACAGTGCCGCGATCATGGAT

Product: metal dependent phosphohydrolase, relA/spoT-like protein

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 188; Mature: 188

Protein sequence:

>188_residues
MNTLSPRLRKAMNTAAWAHRHHVRKGGGIPYVSHLYSVMYLLASVTNDEDVLIAGLLHDTLEDVPEEYNSAQLEADFGPR
VRELVEELTKQPLKSWKARADAYLLHLSAGASLEAVLISTADKLHNLMSILDDLEIHGEDLWQRFNAGKEQQIWWYSEVY
QISLQRLGFNELNKQLGLCVEKLLKQSA

Sequences:

>Translated_188_residues
MNTLSPRLRKAMNTAAWAHRHHVRKGGGIPYVSHLYSVMYLLASVTNDEDVLIAGLLHDTLEDVPEEYNSAQLEADFGPR
VRELVEELTKQPLKSWKARADAYLLHLSAGASLEAVLISTADKLHNLMSILDDLEIHGEDLWQRFNAGKEQQIWWYSEVY
QISLQRLGFNELNKQLGLCVEKLLKQSA
>Mature_188_residues
MNTLSPRLRKAMNTAAWAHRHHVRKGGGIPYVSHLYSVMYLLASVTNDEDVLIAGLLHDTLEDVPEEYNSAQLEADFGPR
VRELVEELTKQPLKSWKARADAYLLHLSAGASLEAVLISTADKLHNLMSILDDLEIHGEDLWQRFNAGKEQQIWWYSEVY
QISLQRLGFNELNKQLGLCVEKLLKQSA

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=129, Percent_Identity=32.5581395348837, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 21380; Mature: 21380

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTLSPRLRKAMNTAAWAHRHHVRKGGGIPYVSHLYSVMYLLASVTNDEDVLIAGLLHDT
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LEDVPEEYNSAQLEADFGPRVRELVEELTKQPLKSWKARADAYLLHLSAGASLEAVLIST
HHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHH
ADKLHNLMSILDDLEIHGEDLWQRFNAGKEQQIWWYSEVYQISLQRLGFNELNKQLGLCV
HHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHH
EKLLKQSA
HHHHHHCC
>Mature Secondary Structure
MNTLSPRLRKAMNTAAWAHRHHVRKGGGIPYVSHLYSVMYLLASVTNDEDVLIAGLLHDT
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LEDVPEEYNSAQLEADFGPRVRELVEELTKQPLKSWKARADAYLLHLSAGASLEAVLIST
HHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHH
ADKLHNLMSILDDLEIHGEDLWQRFNAGKEQQIWWYSEVYQISLQRLGFNELNKQLGLCV
HHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHH
EKLLKQSA
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]