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The map label for this gene is pfkA [H]

Identifier: 62390137

GI number: 62390137

Start: 1316523

End: 1317554

Strand: Direct

Name: pfkA [H]

Synonym: cg1409

Alternate gene names: 62390137

Gene position: 1316523-1317554 (Clockwise)

Preceding gene: 62390135

Following gene: 62390138

Centisome position: 40.1

GC content: 54.55

Gene sequence:

>1032_bases
ATGCGAATTGCTACTCTCACGTCAGGCGGCGACTGCCCCGGACTAAACGCCGTCATCCGAGGAATCGTCCGCACAGCCAG
CAATGAATTTGGCTCCACCGTCGTTGGTTATCAAGACGGTTGGGAAGGACTGTTAGGCGATCGTCGCGTACAGCTGTATG
ACGATGAAGATATTGACCGAATCCTCCTTCGAGGCGGCACCATTTTGGGCACTGGTCGCCTCCATCCGGACAAGTTTAAG
GCCGGAATTGATCAGATTAAGGCCAACTTAGAAGACGCCGGCATCGATGCCCTTATCCCAATCGGTGGCGAAGGAACCCT
GAAGGGTGCCAAGTGGCTGTCTGATAACGGTATCCCTGTTGTCGGTGTCCCAAAGACCATTGACAATGACGTGAATGGCA
CTGACTTCACCTTCGGTTTCGATACTGCTGTGGCAGTGGCTACCGACGCTGTTGACCGCCTGCACACCACCGCTGAATCT
CACAACCGTGTGATGATCGTGGAGGTCATGGGCCGCCACGTGGGTTGGATTGCTCTGCACGCAGGTATGGCCGGCGGTGC
TCACTACACCGTTATTCCAGAAGTACCTTTCGATATTGCAGAGATCTGCAAGGCGATGGAACGTCGCTTCCAGATGGGCG
AGAAGTACGGCATTATCGTCGTTGCGGAAGGTGCGTTGCCACGCGAAGGCACCATGGAGCTTCGTGAAGGCCACATTGAC
CAGTTCGGTCACAAGACCTTCACGGGAATTGGACAGCAGATCGCTGATGAGATCCACGTGCGCCTCGGCCACGATGTTCG
TACGACCGTTCTTGGCCACATTCAACGTGGTGGAACCCCAACTGCTTTCGACCGTGTTCTGGCCACTCGTTATGGTGTTC
GTGCAGCTCGTGCGTGCCATGAGGGAAGCTTTGACAAGGTTGTTGCTTTGAAGGGTGAGAGCATTGAGATGATCACCTTT
GAAGAAGCAGTCGGAACCTTGAAGGAAGTTCCATTCGAACGCTGGGTTACTGCCCAGGCAATGTTTGGATAG

Upstream 100 bases:

>100_bases
ATGGTCAGAGACAGTTTTTTGGCCTGTCAACCCCTGTGATTCTCTTATTTTTGGGTGATTGTTCCGGCGCGGGTGTTGTG
ATGGGTTTAATATGGAAGAC

Downstream 100 bases:

>100_bases
TTTTTCGGGCTTTTATCAACAGCCAATAACAGCTCTTTCGCCCATTGAGGTGGAGGGGCTGTTTTTTCATGCCGTAAGGA
AAGTGCAAGTAAGTGAAATC

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MRIATLTSGGDCPGLNAVIRGIVRTASNEFGSTVVGYQDGWEGLLGDRRVQLYDDEDIDRILLRGGTILGTGRLHPDKFK
AGIDQIKANLEDAGIDALIPIGGEGTLKGAKWLSDNGIPVVGVPKTIDNDVNGTDFTFGFDTAVAVATDAVDRLHTTAES
HNRVMIVEVMGRHVGWIALHAGMAGGAHYTVIPEVPFDIAEICKAMERRFQMGEKYGIIVVAEGALPREGTMELREGHID
QFGHKTFTGIGQQIADEIHVRLGHDVRTTVLGHIQRGGTPTAFDRVLATRYGVRAARACHEGSFDKVVALKGESIEMITF
EEAVGTLKEVPFERWVTAQAMFG

Sequences:

>Translated_343_residues
MRIATLTSGGDCPGLNAVIRGIVRTASNEFGSTVVGYQDGWEGLLGDRRVQLYDDEDIDRILLRGGTILGTGRLHPDKFK
AGIDQIKANLEDAGIDALIPIGGEGTLKGAKWLSDNGIPVVGVPKTIDNDVNGTDFTFGFDTAVAVATDAVDRLHTTAES
HNRVMIVEVMGRHVGWIALHAGMAGGAHYTVIPEVPFDIAEICKAMERRFQMGEKYGIIVVAEGALPREGTMELREGHID
QFGHKTFTGIGQQIADEIHVRLGHDVRTTVLGHIQRGGTPTAFDRVLATRYGVRAARACHEGSFDKVVALKGESIEMITF
EEAVGTLKEVPFERWVTAQAMFG
>Mature_343_residues
MRIATLTSGGDCPGLNAVIRGIVRTASNEFGSTVVGYQDGWEGLLGDRRVQLYDDEDIDRILLRGGTILGTGRLHPDKFK
AGIDQIKANLEDAGIDALIPIGGEGTLKGAKWLSDNGIPVVGVPKTIDNDVNGTDFTFGFDTAVAVATDAVDRLHTTAES
HNRVMIVEVMGRHVGWIALHAGMAGGAHYTVIPEVPFDIAEICKAMERRFQMGEKYGIIVVAEGALPREGTMELREGHID
QFGHKTFTGIGQQIADEIHVRLGHDVRTTVLGHIQRGGTPTAFDRVLATRYGVRAARACHEGSFDKVVALKGESIEMITF
EEAVGTLKEVPFERWVTAQAMFG

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI266453768, Length=358, Percent_Identity=36.5921787709497, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI266453748, Length=358, Percent_Identity=36.5921787709497, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI4505749, Length=358, Percent_Identity=36.5921787709497, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI266453619, Length=358, Percent_Identity=36.5921787709497, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI11321601, Length=358, Percent_Identity=35.4748603351955, Blast_Score=187, Evalue=9e-48,
Organism=Homo sapiens, GI48762920, Length=358, Percent_Identity=34.3575418994413, Blast_Score=178, Evalue=8e-45,
Organism=Escherichia coli, GI1790350, Length=333, Percent_Identity=39.039039039039, Blast_Score=219, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI25147584, Length=328, Percent_Identity=34.4512195121951, Blast_Score=167, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI17558788, Length=353, Percent_Identity=30.5949008498584, Blast_Score=151, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI72003601, Length=304, Percent_Identity=29.9342105263158, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321679, Length=331, Percent_Identity=38.6706948640483, Blast_Score=189, Evalue=6e-49,
Organism=Saccharomyces cerevisiae, GI6323861, Length=361, Percent_Identity=35.4570637119114, Blast_Score=171, Evalue=2e-43,
Organism=Drosophila melanogaster, GI28573326, Length=375, Percent_Identity=35.4666666666667, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24652337, Length=375, Percent_Identity=35.2, Blast_Score=194, Evalue=5e-50,
Organism=Drosophila melanogaster, GI17647809, Length=375, Percent_Identity=35.2, Blast_Score=194, Evalue=1e-49,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 37089; Mature: 37089

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIATLTSGGDCPGLNAVIRGIVRTASNEFGSTVVGYQDGWEGLLGDRRVQLYDDEDIDR
CEEEEECCCCCCCCHHHHHHHHHHHHCCHHCCEEECCCCCCCCCCCCCEEEEECCCCHHH
ILLRGGTILGTGRLHPDKFKAGIDQIKANLEDAGIDALIPIGGEGTLKGAKWLSDNGIPV
HHHCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCEEECCCCCEE
VGVPKTIDNDVNGTDFTFGFDTAVAVATDAVDRLHTTAESHNRVMIVEVMGRHVGWIALH
EECCHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEE
AGMAGGAHYTVIPEVPFDIAEICKAMERRFQMGEKYGIIVVAEGALPREGTMELREGHID
CCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHCCHH
QFGHKTFTGIGQQIADEIHVRLGHDVRTTVLGHIQRGGTPTAFDRVLATRYGVRAARACH
HHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHC
EGSFDKVVALKGESIEMITFEEAVGTLKEVPFERWVTAQAMFG
CCCCCEEEEECCCCEEEEEHHHHHHHHHHCCHHHHHHHHHCCC
>Mature Secondary Structure
MRIATLTSGGDCPGLNAVIRGIVRTASNEFGSTVVGYQDGWEGLLGDRRVQLYDDEDIDR
CEEEEECCCCCCCCHHHHHHHHHHHHCCHHCCEEECCCCCCCCCCCCCEEEEECCCCHHH
ILLRGGTILGTGRLHPDKFKAGIDQIKANLEDAGIDALIPIGGEGTLKGAKWLSDNGIPV
HHHCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCEEECCCCCEE
VGVPKTIDNDVNGTDFTFGFDTAVAVATDAVDRLHTTAESHNRVMIVEVMGRHVGWIALH
EECCHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEE
AGMAGGAHYTVIPEVPFDIAEICKAMERRFQMGEKYGIIVVAEGALPREGTMELREGHID
CCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHCCHH
QFGHKTFTGIGQQIADEIHVRLGHDVRTTVLGHIQRGGTPTAFDRVLATRYGVRAARACH
HHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHC
EGSFDKVVALKGESIEMITFEEAVGTLKEVPFERWVTAQAMFG
CCCCCEEEEECCCCEEEEEHHHHHHHHHHCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]