Click here to switch to the map view.

The map label for this gene is gatA [H]

Identifier: 62390134

GI number: 62390134

Start: 1313093

End: 1314586

Strand: Direct

Name: gatA [H]

Synonym: cg1404

Alternate gene names: 62390134

Gene position: 1313093-1314586 (Clockwise)

Preceding gene: 62390133

Following gene: 62390135

Centisome position: 40.0

GC content: 58.3

Gene sequence:

>1494_bases
ATGACCAACAAGTACCTGGTTGAAGGCTCTGAAAACGAGCTGACCACAAAGACCGCAGCAGAGCTGGCAGGTCTTATTCA
TTCCCGCGAGGTAACTTCCCGCGAGGTTACTCAAGCGCACCTAGATCGCATTGCTGCGGTTGACGGCGATATTCATGCAT
TTCTCCACGTTGGCCAGGAGGAGGCCCTGAACGCGGCGGATGACGTCGATAAGCGTCTAGACGCTGGAGAGGCACCTGCC
TCGGCTTTGGCTGGCGTGCCGCTTGCGCTGAAGGATGTCTTTACCACCACTGATGCGCCGACCACGGCGGCATCGAAGAT
GCTTGAGGGCTACATGAGCCCTTATGACGCGACTGTGACCCGCAAGATCCGTGAGGCTGGCATCCCAATTTTGGGTAAGA
CCAACATGGATGAGTTTGCGATGGGTTCCTCCACTGAGAACTCCGCATACGGCCCAACCCACAATCCGTGGGATCTGGAG
CGCACCGCAGGTGGTTCTGGTGGTGGCTCTTCAGCTGCTCTTGCTGCAGGTCAGGCGCCACTTGCGATTGGTACTGACAC
TGGTGGATCCATCCGTCAGCCAGCTGCGCTGACCAACACTGTCGGTGTGAAGCCAACCTACGGAACCGTATCCCGTTACG
GTCTGATTGCGTGTGCGTCCTCCCTGGATCAGGGTGGCCCAACCGCTCGTACTGTTCTGGATACCGCGCTTTTGCACGAG
GTTATCGCAGGCCACGACGCTTTTGATGCGACCTCCGTGAATCGTCCGGTTGCTCCTGTTGTGCAGGCTGCCCGTGAAGG
CGCGAACGGTGACCTGAAAGGCGTGAAGGTCGGTGTGGTCAAGCAGTTCGACCGCGACGGCTACCAGCCTGGCGTGCTTG
AGGCATTCCACGCTTCTGTTGAGCAGATGCGCTCCCAGGGTGCGGAAATCGTCGAGGTTGATTGCCCTCACTTTGATGAC
GCTCTTGGCGCGTACTACCTGATTCTTCCTTGTGAAGTTTCCTCCAACCTCGCGCGTTTTGACGGCATGCGTTACGGTTT
GCGCGCTGGTGATGACGGAACTCGTTCCGCCGATGAGGTCATGGCGTACACCCGTGCGCAGGGATTCGGCCCTGAGGTTA
AGCGCCGTATCATCCTCGGCACTTACGCGTTGTCTGTTGGTTACTACGACGCGTACTACCTGCAGGCTCAGCGCGTTCGT
ACCCTCATTGCACAGGACTTCGCCAAGGCTTACGAGCAGGTCGACATCTTGGTGTCCCCAACCACTCCAACCACCGCGTT
CAAGCTGGGGGAGAAGGTCACCGATCCGCTGGAGATGTACAACTTCGACTTGTGCACCCTGCCACTGAACCTGGCTGGTC
TCGCGGGCATGTCCCTGCCTTCCGGCTTGGCATCAGATACTGGTCTGCCTGTTGGTTTGCAGCTGATGGCTCCTGCTTTC
CAGGACGATCGTCTCTACCGCGTCGGCGCTGCTTTTGAAGCTGGACGCAAGTAG

Upstream 100 bases:

>100_bases
ACAAGACCCTCGATGCTGCGGCTGCGTTGGACCAAGCGCCCGCTGTCGAGGATGGACGTTTTATGGTTCCGCAGATTCTG
GGTGAGGGCGACTAATAATT

Downstream 100 bases:

>100_bases
GTTCTAAACCCTTTTTAAGAAATTGGCTGCCCTCGCATGACTGTGCGCGGGCGGCCAATTTTTTCTTTTGTGGGGGTGCC
TCTTACGCATTTCCTGAATT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MTNKYLVEGSENELTTKTAAELAGLIHSREVTSREVTQAHLDRIAAVDGDIHAFLHVGQEEALNAADDVDKRLDAGEAPA
SALAGVPLALKDVFTTTDAPTTAASKMLEGYMSPYDATVTRKIREAGIPILGKTNMDEFAMGSSTENSAYGPTHNPWDLE
RTAGGSGGGSSAALAAGQAPLAIGTDTGGSIRQPAALTNTVGVKPTYGTVSRYGLIACASSLDQGGPTARTVLDTALLHE
VIAGHDAFDATSVNRPVAPVVQAAREGANGDLKGVKVGVVKQFDRDGYQPGVLEAFHASVEQMRSQGAEIVEVDCPHFDD
ALGAYYLILPCEVSSNLARFDGMRYGLRAGDDGTRSADEVMAYTRAQGFGPEVKRRIILGTYALSVGYYDAYYLQAQRVR
TLIAQDFAKAYEQVDILVSPTTPTTAFKLGEKVTDPLEMYNFDLCTLPLNLAGLAGMSLPSGLASDTGLPVGLQLMAPAF
QDDRLYRVGAAFEAGRK

Sequences:

>Translated_497_residues
MTNKYLVEGSENELTTKTAAELAGLIHSREVTSREVTQAHLDRIAAVDGDIHAFLHVGQEEALNAADDVDKRLDAGEAPA
SALAGVPLALKDVFTTTDAPTTAASKMLEGYMSPYDATVTRKIREAGIPILGKTNMDEFAMGSSTENSAYGPTHNPWDLE
RTAGGSGGGSSAALAAGQAPLAIGTDTGGSIRQPAALTNTVGVKPTYGTVSRYGLIACASSLDQGGPTARTVLDTALLHE
VIAGHDAFDATSVNRPVAPVVQAAREGANGDLKGVKVGVVKQFDRDGYQPGVLEAFHASVEQMRSQGAEIVEVDCPHFDD
ALGAYYLILPCEVSSNLARFDGMRYGLRAGDDGTRSADEVMAYTRAQGFGPEVKRRIILGTYALSVGYYDAYYLQAQRVR
TLIAQDFAKAYEQVDILVSPTTPTTAFKLGEKVTDPLEMYNFDLCTLPLNLAGLAGMSLPSGLASDTGLPVGLQLMAPAF
QDDRLYRVGAAFEAGRK
>Mature_496_residues
TNKYLVEGSENELTTKTAAELAGLIHSREVTSREVTQAHLDRIAAVDGDIHAFLHVGQEEALNAADDVDKRLDAGEAPAS
ALAGVPLALKDVFTTTDAPTTAASKMLEGYMSPYDATVTRKIREAGIPILGKTNMDEFAMGSSTENSAYGPTHNPWDLER
TAGGSGGGSSAALAAGQAPLAIGTDTGGSIRQPAALTNTVGVKPTYGTVSRYGLIACASSLDQGGPTARTVLDTALLHEV
IAGHDAFDATSVNRPVAPVVQAAREGANGDLKGVKVGVVKQFDRDGYQPGVLEAFHASVEQMRSQGAEIVEVDCPHFDDA
LGAYYLILPCEVSSNLARFDGMRYGLRAGDDGTRSADEVMAYTRAQGFGPEVKRRIILGTYALSVGYYDAYYLQAQRVRT
LIAQDFAKAYEQVDILVSPTTPTTAFKLGEKVTDPLEMYNFDLCTLPLNLAGLAGMSLPSGLASDTGLPVGLQLMAPAFQ
DDRLYRVGAAFEAGRK

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=501, Percent_Identity=34.9301397205589, Blast_Score=281, Evalue=1e-75,
Organism=Homo sapiens, GI195972892, Length=512, Percent_Identity=25.9765625, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI166795287, Length=218, Percent_Identity=29.8165137614679, Blast_Score=68, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17543272, Length=423, Percent_Identity=36.8794326241135, Blast_Score=242, Evalue=4e-64,
Organism=Caenorhabditis elegans, GI17537465, Length=523, Percent_Identity=24.0917782026769, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17556264, Length=309, Percent_Identity=25.5663430420712, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17538252, Length=308, Percent_Identity=24.025974025974, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71990152, Length=331, Percent_Identity=26.5861027190332, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17556276, Length=315, Percent_Identity=27.6190476190476, Blast_Score=69, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17556278, Length=315, Percent_Identity=27.6190476190476, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=414, Percent_Identity=34.5410628019324, Blast_Score=223, Evalue=8e-59,
Organism=Saccharomyces cerevisiae, GI6319685, Length=430, Percent_Identity=30, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6320448, Length=207, Percent_Identity=29.4685990338164, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24648113, Length=458, Percent_Identity=41.703056768559, Blast_Score=308, Evalue=7e-84,
Organism=Drosophila melanogaster, GI24644968, Length=522, Percent_Identity=25.4789272030651, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI21356731, Length=244, Percent_Identity=32.7868852459016, Blast_Score=115, Evalue=9e-26,
Organism=Drosophila melanogaster, GI24652985, Length=498, Percent_Identity=24.8995983935743, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI19922090, Length=498, Percent_Identity=24.8995983935743, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24652981, Length=498, Percent_Identity=24.8995983935743, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24652983, Length=498, Percent_Identity=24.8995983935743, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI45550774, Length=202, Percent_Identity=34.1584158415842, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24648435, Length=202, Percent_Identity=34.1584158415842, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24648437, Length=202, Percent_Identity=34.1584158415842, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI161078093, Length=524, Percent_Identity=25.5725190839695, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648441, Length=134, Percent_Identity=35.8208955223881, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648439, Length=134, Percent_Identity=35.8208955223881, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52536; Mature: 52405

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNKYLVEGSENELTTKTAAELAGLIHSREVTSREVTQAHLDRIAAVDGDIHAFLHVGQE
CCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHH
EALNAADDVDKRLDAGEAPASALAGVPLALKDVFTTTDAPTTAASKMLEGYMSPYDATVT
HHCCCHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHH
RKIREAGIPILGKTNMDEFAMGSSTENSAYGPTHNPWDLERTAGGSGGGSSAALAAGQAP
HHHHHCCCCEEECCCCHHHHCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCEEECCCCC
LAIGTDTGGSIRQPAALTNTVGVKPTYGTVSRYGLIACASSLDQGGPTARTVLDTALLHE
EEEECCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
VIAGHDAFDATSVNRPVAPVVQAAREGANGDLKGVKVGVVKQFDRDGYQPGVLEAFHASV
HHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEHHHHHHCCCCCCCCHHHHHHHHH
EQMRSQGAEIVEVDCPHFDDALGAYYLILPCEVSSNLARFDGMRYGLRAGDDGTRSADEV
HHHHHCCCEEEEECCCCCCCCCCEEEEEEEECCCCCHHHHCCCCCCCCCCCCCCCCHHHH
MAYTRAQGFGPEVKRRIILGTYALSVGYYDAYYLQAQRVRTLIAQDFAKAYEQVDILVSP
HHHHHHCCCCHHHHHEEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEC
TTPTTAFKLGEKVTDPLEMYNFDLCTLPLNLAGLAGMSLPSGLASDTGLPVGLQLMAPAF
CCCCHHHHHCHHCCCHHHHHCCCEEECCCHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCC
QDDRLYRVGAAFEAGRK
CCCCEEEECCHHHCCCC
>Mature Secondary Structure 
TNKYLVEGSENELTTKTAAELAGLIHSREVTSREVTQAHLDRIAAVDGDIHAFLHVGQE
CCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHH
EALNAADDVDKRLDAGEAPASALAGVPLALKDVFTTTDAPTTAASKMLEGYMSPYDATVT
HHCCCHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHH
RKIREAGIPILGKTNMDEFAMGSSTENSAYGPTHNPWDLERTAGGSGGGSSAALAAGQAP
HHHHHCCCCEEECCCCHHHHCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCEEECCCCC
LAIGTDTGGSIRQPAALTNTVGVKPTYGTVSRYGLIACASSLDQGGPTARTVLDTALLHE
EEEECCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
VIAGHDAFDATSVNRPVAPVVQAAREGANGDLKGVKVGVVKQFDRDGYQPGVLEAFHASV
HHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEHHHHHHCCCCCCCCHHHHHHHHH
EQMRSQGAEIVEVDCPHFDDALGAYYLILPCEVSSNLARFDGMRYGLRAGDDGTRSADEV
HHHHHCCCEEEEECCCCCCCCCCEEEEEEEECCCCCHHHHCCCCCCCCCCCCCCCCHHHH
MAYTRAQGFGPEVKRRIILGTYALSVGYYDAYYLQAQRVRTLIAQDFAKAYEQVDILVSP
HHHHHHCCCCHHHHHEEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEC
TTPTTAFKLGEKVTDPLEMYNFDLCTLPLNLAGLAGMSLPSGLASDTGLPVGLQLMAPAF
CCCCHHHHHCHHCCCHHHHHCCCEEECCCHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCC
QDDRLYRVGAAFEAGRK
CCCCEEEECCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]