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The map label for this gene is rrmA [H]

Identifier: 62390005

GI number: 62390005

Start: 1161333

End: 1162199

Strand: Direct

Name: rrmA [H]

Synonym: cg1266

Alternate gene names: 62390005

Gene position: 1161333-1162199 (Clockwise)

Preceding gene: 62390004

Following gene: 62390008

Centisome position: 35.38

GC content: 55.82

Gene sequence:

>867_bases
GTGCTTTCCCACATCATTGATGTCCTCGCCGACCCGATCGATGGCACCCCACTTGTAGGCGCCGAAGATTTCTCACGGTT
GGTGTCTGAATCTGGGCATTCCTACGATGTTGCTCGTCAAGGGTATGTCACCCTGGCTGGTGGCGCAGGTCTGCGCTATT
CAGGCGATGATGCACAGATGATCGCGGATCGGGAAACCTTCCTTTCTGGCGGTCACTTCGCGCCCTTCGTGGAAGCTGTC
ACCGAGCATGTTCAAGATGTCGTTGACCAGGCAGGCCTTAGCGATGACGCACAGCCAGTGGTCTGCGAAATCGGCGCGGG
AACCGGCTACTACTTGTCCCATACCCTTGATTCTGTTGCAGGATCTCGCGGAATTGGCATTGACGTTTCCGTGCACGCCG
CAAAGCGTTTGGCAAAGTGTCACCCTCGCGTCGGCGCAGTCATCGCGGACGCATGGGCACGCCTGCCGATTGCAGATAAC
TCTGTCGATGCCATCACGGTGATTTTCGCACCACGCAACGCTGCGGAATTTGCCCGCATTTTGAAGCCAAAGGGACAGGT
CATCGTGCTTACCGCAGATACCGGCCACTTGGCTGAGCTTCGTGAACCACTGGGCATCATTGATGTGGAGGCCGGCAAAG
TTGATCGCATGATCGAACAAGCGGCAGGCCACCTCAAGCCAGTTGGCGAAAGAGACTTGGTGGAATTTGAAATGCTGCTG
GATCAAAAATCCATTGCATCTCAGATCGGTATGAGCCCTTCTGCACGCCACATTAAGCCTGAGGCTTTGGCGGAACGCAT
CGCCGCTCTACCAGAACAAATGAAGGTTACAGCCCGGGCCAAGATCACCAGGCTGGAACGCATCTAA

Upstream 100 bases:

>100_bases
CCGAACTTGGAGCTTTGCTGTTGGAGGCAGCCAAATAGTCCCAATGTAAACGCACTGGGTAGTATTTGTTTAACCATCCA
CCTCAAGGAGTAAAACGCAC

Downstream 100 bases:

>100_bases
CTCTTATCTCACTGGGCCTTCATGCGGTTCGTTTTCATCGAACTGCATGAGCGCAGTGAGATCATCTATATCTAGGGACA
CCGAACTGAAATCAACAGGG

Product: rRNA guanine-N1-methyltransferase

Products: S-adenosyl-L-homocysteine +rRNA containing Nmethylguanine [C]

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MLSHIIDVLADPIDGTPLVGAEDFSRLVSESGHSYDVARQGYVTLAGGAGLRYSGDDAQMIADRETFLSGGHFAPFVEAV
TEHVQDVVDQAGLSDDAQPVVCEIGAGTGYYLSHTLDSVAGSRGIGIDVSVHAAKRLAKCHPRVGAVIADAWARLPIADN
SVDAITVIFAPRNAAEFARILKPKGQVIVLTADTGHLAELREPLGIIDVEAGKVDRMIEQAAGHLKPVGERDLVEFEMLL
DQKSIASQIGMSPSARHIKPEALAERIAALPEQMKVTARAKITRLERI

Sequences:

>Translated_288_residues
MLSHIIDVLADPIDGTPLVGAEDFSRLVSESGHSYDVARQGYVTLAGGAGLRYSGDDAQMIADRETFLSGGHFAPFVEAV
TEHVQDVVDQAGLSDDAQPVVCEIGAGTGYYLSHTLDSVAGSRGIGIDVSVHAAKRLAKCHPRVGAVIADAWARLPIADN
SVDAITVIFAPRNAAEFARILKPKGQVIVLTADTGHLAELREPLGIIDVEAGKVDRMIEQAAGHLKPVGERDLVEFEMLL
DQKSIASQIGMSPSARHIKPEALAERIAALPEQMKVTARAKITRLERI
>Mature_288_residues
MLSHIIDVLADPIDGTPLVGAEDFSRLVSESGHSYDVARQGYVTLAGGAGLRYSGDDAQMIADRETFLSGGHFAPFVEAV
TEHVQDVVDQAGLSDDAQPVVCEIGAGTGYYLSHTLDSVAGSRGIGIDVSVHAAKRLAKCHPRVGAVIADAWARLPIADN
SVDAITVIFAPRNAAEFARILKPKGQVIVLTADTGHLAELREPLGIIDVEAGKVDRMIEQAAGHLKPVGERDLVEFEMLL
DQKSIASQIGMSPSARHIKPEALAERIAALPEQMKVTARAKITRLERI

Specific function: Confers strong resistance to mycinamicin (MM) and tylosin (TY). May function as methyltransferase [H]

COG id: COG0500

COG function: function code QR; SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmA family [H]

Homologues:

Organism=Escherichia coli, GI1788125, Length=253, Percent_Identity=33.201581027668, Blast_Score=112, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013216
- InterPro:   IPR016718 [H]

Pfam domain/function: PF08241 Methyltransf_11 [H]

EC number: 2.1.1.51 [C]

Molecular weight: Translated: 30710; Mature: 30710

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSHIIDVLADPIDGTPLVGAEDFSRLVSESGHSYDVARQGYVTLAGGAGLRYSGDDAQM
CHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCEECCCCCEEEEECCCCCEECCCCHHH
IADRETFLSGGHFAPFVEAVTEHVQDVVDQAGLSDDAQPVVCEIGAGTGYYLSHTLDSVA
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHC
GSRGIGIDVSVHAAKRLAKCHPRVGAVIADAWARLPIADNSVDAITVIFAPRNAAEFARI
CCCCCCEEEEHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHH
LKPKGQVIVLTADTGHLAELREPLGIIDVEAGKVDRMIEQAAGHLKPVGERDLVEFEMLL
HCCCCCEEEEEECCCCHHHHHCCCCEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHH
DQKSIASQIGMSPSARHIKPEALAERIAALPEQMKVTARAKITRLERI
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLSHIIDVLADPIDGTPLVGAEDFSRLVSESGHSYDVARQGYVTLAGGAGLRYSGDDAQM
CHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCEECCCCCEEEEECCCCCEECCCCHHH
IADRETFLSGGHFAPFVEAVTEHVQDVVDQAGLSDDAQPVVCEIGAGTGYYLSHTLDSVA
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHC
GSRGIGIDVSVHAAKRLAKCHPRVGAVIADAWARLPIADNSVDAITVIFAPRNAAEFARI
CCCCCCEEEEHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHH
LKPKGQVIVLTADTGHLAELREPLGIIDVEAGKVDRMIEQAAGHLKPVGERDLVEFEMLL
HCCCCCEEEEEECCCCHHHHHCCCCEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHH
DQKSIASQIGMSPSARHIKPEALAERIAALPEQMKVTARAKITRLERI
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Unidentified protein factor B [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; rRNA [C]

Specific reaction: S-Adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine +rRNA containing Nmethylguanine [C]

General reaction: Methyl group transfer [C]

Inhibitor: Mg2+; Unidentified protein factor A1 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8163173 [H]