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The map label for this gene is ybjI [H]

Identifier: 62389808

GI number: 62389808

Start: 973711

End: 974505

Strand: Reverse

Name: ybjI [H]

Synonym: cg1048

Alternate gene names: 62389808

Gene position: 974505-973711 (Counterclockwise)

Preceding gene: 62389811

Following gene: 62389804

Centisome position: 29.69

GC content: 54.09

Gene sequence:

>795_bases
ATGGACTTTCGCCTCGTCGCGACAGACATGGACGGCACACTTTTAAACACCCACCACGAAGTCCCAGAGAAATTTTGGGA
CATCCTGGAACAAATGCGTGCCAAAGGAATCGCCTTCGCACCAGCCAGCGGCCGTCAATTAGCCACCTTGCAAAAACAAT
TCGGGCACGCGGGTGAACCCATTTCTTACATCGCAGAAAACGGCACCGTGGTAGTCCACGACGGCGAAATTATCTCCCTG
ACCACCATCGACTCCGACACCGTACACTCCATCATCGATGCCGTGCGCGCATCCGACATCGATATGGGAGTAGTGGTCTG
CCGACCAGAACGCGCCTACGTCGAACGCAACGACGAAGCTTTCCGCGCCGAAGGCCTGAAATACTACGTCTCCATCGAGG
AAGTCCAAGACCTCCACGAAGCAGTCAACAATGAAGTAATCAAGGTAGCGATCTTTACATTCCAAGATGCCGAAAAGGAC
TGTGCCCCCATCATCCGCGCAGCCTCCCCCAACGCCAACGTTGTTGTCTCCGGCCAGCACTGGGTCGATGTCATGGATCC
TTCAGCCAACAAGGGCCAAGCTTTGGCTGCTCTCCGCGATGCCCTCGGATTGGAAGAATCCCAAACTCTCGTGTTTGGCG
ACTACCTCAACGACACTGAATTGATCAAGGCCGCCGGCAAGTCTTACGCCATGTCCAATGCCCACCCGGACATTTTGGAA
TTGGCCGACGAAATTGCACCATCCAACATTGAAGAGGGCGTTATTGTGGTGCTGGAGAAGTTGCTTAACGGTTAA

Upstream 100 bases:

>100_bases
AACCAGTGTGGCACATCACAGGAAATCTTCGCGGGTGTTTAGACAACCCGGATGTGACAGAATGGGCGATCAAGACATAA
TCGGAAAGGAATCCAAACAA

Downstream 100 bases:

>100_bases
CGATTGCAGGCAGCAGGTTCAGGCGAAATGGTTAGGATCCACCACAGATCCGTCGATTCTTAGGCGGTGTGGTGAGTCCT
TGCCCTGGAGCCTTGCAGGA

Product: HAD family hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MDFRLVATDMDGTLLNTHHEVPEKFWDILEQMRAKGIAFAPASGRQLATLQKQFGHAGEPISYIAENGTVVVHDGEIISL
TTIDSDTVHSIIDAVRASDIDMGVVVCRPERAYVERNDEAFRAEGLKYYVSIEEVQDLHEAVNNEVIKVAIFTFQDAEKD
CAPIIRAASPNANVVVSGQHWVDVMDPSANKGQALAALRDALGLEESQTLVFGDYLNDTELIKAAGKSYAMSNAHPDILE
LADEIAPSNIEEGVIVVLEKLLNG

Sequences:

>Translated_264_residues
MDFRLVATDMDGTLLNTHHEVPEKFWDILEQMRAKGIAFAPASGRQLATLQKQFGHAGEPISYIAENGTVVVHDGEIISL
TTIDSDTVHSIIDAVRASDIDMGVVVCRPERAYVERNDEAFRAEGLKYYVSIEEVQDLHEAVNNEVIKVAIFTFQDAEKD
CAPIIRAASPNANVVVSGQHWVDVMDPSANKGQALAALRDALGLEESQTLVFGDYLNDTELIKAAGKSYAMSNAHPDILE
LADEIAPSNIEEGVIVVLEKLLNG
>Mature_264_residues
MDFRLVATDMDGTLLNTHHEVPEKFWDILEQMRAKGIAFAPASGRQLATLQKQFGHAGEPISYIAENGTVVVHDGEIISL
TTIDSDTVHSIIDAVRASDIDMGVVVCRPERAYVERNDEAFRAEGLKYYVSIEEVQDLHEAVNNEVIKVAIFTFQDAEKD
CAPIIRAASPNANVVVSGQHWVDVMDPSANKGQALAALRDALGLEESQTLVFGDYLNDTELIKAAGKSYAMSNAHPDILE
LADEIAPSNIEEGVIVVLEKLLNG

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates FMN and beta-glucose 1-phosphate [H]

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI87081790, Length=270, Percent_Identity=30, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1787043, Length=276, Percent_Identity=28.9855072463768, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI2367265, Length=270, Percent_Identity=26.6666666666667, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI48994981, Length=274, Percent_Identity=29.5620437956204, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87081741, Length=241, Percent_Identity=25.7261410788382, Blast_Score=75, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 28830; Mature: 28830

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: PS01228 COF_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFRLVATDMDGTLLNTHHEVPEKFWDILEQMRAKGIAFAPASGRQLATLQKQFGHAGEP
CCEEEEEECCCCCEECCCHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHCCCCCC
ISYIAENGTVVVHDGEIISLTTIDSDTVHSIIDAVRASDIDMGVVVCRPERAYVERNDEA
HHEEECCCEEEEECCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHCCCCHH
FRAEGLKYYVSIEEVQDLHEAVNNEVIKVAIFTFQDAEKDCAPIIRAASPNANVVVSGQH
HHHCCEEEEEEHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHEECCCCCCCEEECCCC
WVDVMDPSANKGQALAALRDALGLEESQTLVFGDYLNDTELIKAAGKSYAMSNAHPDILE
EEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHCCCCCCCCCCCCHHHH
LADEIAPSNIEEGVIVVLEKLLNG
HHHHHCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MDFRLVATDMDGTLLNTHHEVPEKFWDILEQMRAKGIAFAPASGRQLATLQKQFGHAGEP
CCEEEEEECCCCCEECCCHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHCCCCCC
ISYIAENGTVVVHDGEIISLTTIDSDTVHSIIDAVRASDIDMGVVVCRPERAYVERNDEA
HHEEECCCEEEEECCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHCCCCHH
FRAEGLKYYVSIEEVQDLHEAVNNEVIKVAIFTFQDAEKDCAPIIRAASPNANVVVSGQH
HHHCCEEEEEEHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHEECCCCCCCEEECCCC
WVDVMDPSANKGQALAALRDALGLEESQTLVFGDYLNDTELIKAAGKSYAMSNAHPDILE
EEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHCCCCCCCCCCCCHHHH
LADEIAPSNIEEGVIVVLEKLLNG
HHHHHCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10493123 [H]