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The map label for this gene is gltA

Identifier: 62389719

GI number: 62389719

Start: 879309

End: 880622

Strand: Direct

Name: gltA

Synonym: cg0949

Alternate gene names: 62389719

Gene position: 879309-880622 (Clockwise)

Preceding gene: 62389715

Following gene: 62389720

Centisome position: 26.79

GC content: 55.02

Gene sequence:

>1314_bases
ATGTTTGAAAGGGATATCGTGGCTACTGATAACAACAAGGCTGTCCTGCACTACCCCGGTGGCGAGTTCGAAATGGACAT
CATCGAGGCTTCTGAGGGTAACAACGGTGTTGTCCTGGGCAAGATGCTGTCTGAGACTGGACTGATCACTTTTGACCCAG
GTTATGTGAGCACTGGCTCCACCGAGTCGAAGATCACCTACATCGATGGCGATGCGGGAATCCTGCGTTACCGCGGCTAT
GACATCGCTGATCTGGCTGAGAATGCCACCTTCAACGAGGTTTCTTACCTACTTATCAACGGTGAGCTACCAACCCCAGA
TGAGCTTCACAAGTTTAACGACGAGATTCGCCACCACACCCTTCTGGACGAGGACTTCAAGTCCCAGTTCAACGTGTTCC
CACGCGACGCTCACCCAATGGCAACCTTGGCTTCCTCGGTTAACATTTTGTCTACCTACTACCAGGACCAGCTGAACCCA
CTCGATGAGGCACAGCTTGATAAGGCAACCGTTCGCCTCATGGCAAAGGTTCCAATGCTGGCTGCGTACGCACACCGCGC
ACGCAAGGGTGCTCCTTACATGTACCCAGACAACTCCCTCAATGCGCGTGAGAACTTCCTGCGCATGATGTTCGGTTACC
CAACCGAGCCATACGAGATCGACCCAATCATGGTCAAGGCTCTGGACAAGCTGCTCATCCTGCACGCTGACCACGAGCAG
AACTGCTCCACCTCCACCGTTCGTATGATCGGTTCCGCACAGGCCAACATGTTTGTCTCCATCGCTGGTGGCATCAACGC
TCTGTCCGGCCCACTGCACGGTGGCGCAAACCAGGCTGTTCTGGAGATGCTCGAAGACATCAAGAGCAACCACGGTGGCG
ACGCAACCGAGTTCATGAACAAGGTCAAGAACAAGGAAGACGGCGTCCGCCTCATGGGCTTCGGACACCGCGTTTACAAG
AACTACGATCCACGTGCAGCAATCGTCAAGGAGACCGCACACGAGATCCTCGAGCACCTCGGTGGCGACGATCTTCTGGA
TCTGGCAATCAAGCTGGAAGAAATTGCACTGGCTGATGATTACTTCATCTCCCGCAAGCTCTACCCGAACGTAGACTTCT
ACACCGGCCTGATCTACCGCGCAATGGGCTTCCCAACTGACTTCTTCACCGTATTGTTCGCAATCGGTCGTCTGCCAGGA
TGGATCGCTCACTACCGCGAGCAGCTCGGTGCAGCAGGCAACAAGATCAACCGCCCACGCCAGGTCTACACCGGCAACGA
ATCCCGCAAGTTGGTTCCTCGCGAGGAGCGCTAA

Upstream 100 bases:

>100_bases
GTGTCGAGTAACAAAAACTGACATGCGCTTGGCGCATCCCAGTTGGTAAGAATAAACGGGACTACTTCCGTAATCCGGAA
GAGTTTTTTTCCGAACAAAT

Downstream 100 bases:

>100_bases
ATTTAGCGGATGATTCTCGTTCAACTTCGGCCGAAGCCACTTCGTCTGTCATAATGACAGGGATGGTTTCGGCCGTTTTT
GCATGAAACCAAAAAATACG

Product: type II citrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MFERDIVATDNNKAVLHYPGGEFEMDIIEASEGNNGVVLGKMLSETGLITFDPGYVSTGSTESKITYIDGDAGILRYRGY
DIADLAENATFNEVSYLLINGELPTPDELHKFNDEIRHHTLLDEDFKSQFNVFPRDAHPMATLASSVNILSTYYQDQLNP
LDEAQLDKATVRLMAKVPMLAAYAHRARKGAPYMYPDNSLNARENFLRMMFGYPTEPYEIDPIMVKALDKLLILHADHEQ
NCSTSTVRMIGSAQANMFVSIAGGINALSGPLHGGANQAVLEMLEDIKSNHGGDATEFMNKVKNKEDGVRLMGFGHRVYK
NYDPRAAIVKETAHEILEHLGGDDLLDLAIKLEEIALADDYFISRKLYPNVDFYTGLIYRAMGFPTDFFTVLFAIGRLPG
WIAHYREQLGAAGNKINRPRQVYTGNESRKLVPREER

Sequences:

>Translated_437_residues
MFERDIVATDNNKAVLHYPGGEFEMDIIEASEGNNGVVLGKMLSETGLITFDPGYVSTGSTESKITYIDGDAGILRYRGY
DIADLAENATFNEVSYLLINGELPTPDELHKFNDEIRHHTLLDEDFKSQFNVFPRDAHPMATLASSVNILSTYYQDQLNP
LDEAQLDKATVRLMAKVPMLAAYAHRARKGAPYMYPDNSLNARENFLRMMFGYPTEPYEIDPIMVKALDKLLILHADHEQ
NCSTSTVRMIGSAQANMFVSIAGGINALSGPLHGGANQAVLEMLEDIKSNHGGDATEFMNKVKNKEDGVRLMGFGHRVYK
NYDPRAAIVKETAHEILEHLGGDDLLDLAIKLEEIALADDYFISRKLYPNVDFYTGLIYRAMGFPTDFFTVLFAIGRLPG
WIAHYREQLGAAGNKINRPRQVYTGNESRKLVPREER
>Mature_437_residues
MFERDIVATDNNKAVLHYPGGEFEMDIIEASEGNNGVVLGKMLSETGLITFDPGYVSTGSTESKITYIDGDAGILRYRGY
DIADLAENATFNEVSYLLINGELPTPDELHKFNDEIRHHTLLDEDFKSQFNVFPRDAHPMATLASSVNILSTYYQDQLNP
LDEAQLDKATVRLMAKVPMLAAYAHRARKGAPYMYPDNSLNARENFLRMMFGYPTEPYEIDPIMVKALDKLLILHADHEQ
NCSTSTVRMIGSAQANMFVSIAGGINALSGPLHGGANQAVLEMLEDIKSNHGGDATEFMNKVKNKEDGVRLMGFGHRVYK
NYDPRAAIVKETAHEILEHLGGDDLLDLAIKLEEIALADDYFISRKLYPNVDFYTGLIYRAMGFPTDFFTVLFAIGRLPG
WIAHYREQLGAAGNKINRPRQVYTGNESRKLVPREER

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family

Homologues:

Organism=Homo sapiens, GI38327625, Length=387, Percent_Identity=27.906976744186, Blast_Score=131, Evalue=2e-30,
Organism=Escherichia coli, GI1786939, Length=407, Percent_Identity=50.6142506142506, Blast_Score=427, Evalue=1e-121,
Organism=Escherichia coli, GI1786527, Length=367, Percent_Identity=34.3324250681199, Blast_Score=198, Evalue=7e-52,
Organism=Caenorhabditis elegans, GI17555174, Length=415, Percent_Identity=27.2289156626506, Blast_Score=124, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6319850, Length=411, Percent_Identity=27.9805352798054, Blast_Score=124, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6324328, Length=432, Percent_Identity=28.2407407407407, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6325257, Length=374, Percent_Identity=27.5401069518717, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24640126, Length=387, Percent_Identity=29.1989664082687, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24640124, Length=387, Percent_Identity=29.1989664082687, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI21356863, Length=390, Percent_Identity=26.4102564102564, Blast_Score=103, Evalue=3e-22,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CISY_CORGL (P42457)

Other databases:

- EMBL:   X66112
- EMBL:   BA000036
- EMBL:   BX927150
- PIR:   I40717
- RefSeq:   NP_600058.1
- RefSeq:   YP_225121.1
- ProteinModelPortal:   P42457
- SMR:   P42457
- GeneID:   1018824
- GeneID:   3344398
- GenomeReviews:   BA000036_GR
- GenomeReviews:   BX927147_GR
- KEGG:   cgb:cg0949
- KEGG:   cgl:NCgl0795
- HOGENOM:   HBG649489
- OMA:   FATPCEP
- PhylomeDB:   P42457
- ProtClustDB:   PRK05614
- BioCyc:   CGLU196627:CG0949-MONOMER
- BRENDA:   2.3.3.1
- GO:   GO:0005737
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953
- Gene3D:   G3DSA:1.10.580.10
- Gene3D:   G3DSA:1.10.230.10
- PANTHER:   PTHR11739
- PRINTS:   PR00143
- TIGRFAMs:   TIGR01798

Pfam domain/function: PF00285 Citrate_synt; SSF48256 Citrate_synthase_core

EC number: =2.3.3.1

Molecular weight: Translated: 48930; Mature: 48930

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: ACT_SITE 316-316 ACT_SITE 372-372

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFERDIVATDNNKAVLHYPGGEFEMDIIEASEGNNGVVLGKMLSETGLITFDPGYVSTGS
CCCCCEEEECCCCEEEECCCCCEEEEEEEECCCCCCEEEEEEHHHCCEEEECCCCCCCCC
TESKITYIDGDAGILRYRGYDIADLAENATFNEVSYLLINGELPTPDELHKFNDEIRHHT
CCCEEEEEECCCCEEEECCCCHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHH
LLDEDFKSQFNVFPRDAHPMATLASSVNILSTYYQDQLNPLDEAQLDKATVRLMAKVPML
HCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
AAYAHRARKGAPYMYPDNSLNARENFLRMMFGYPTEPYEIDPIMVKALDKLLILHADHEQ
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHEEEECCCCC
NCSTSTVRMIGSAQANMFVSIAGGINALSGPLHGGANQAVLEMLEDIKSNHGGDATEFMN
CCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHH
KVKNKEDGVRLMGFGHRVYKNYDPRAAIVKETAHEILEHLGGDDLLDLAIKLEEIALADD
HHCCCCCCEEEEECCHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
YFISRKLYPNVDFYTGLIYRAMGFPTDFFTVLFAIGRLPGWIAHYREQLGAAGNKINRPR
HHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCC
QVYTGNESRKLVPREER
EEEECCCCCCCCCCCCC
>Mature Secondary Structure
MFERDIVATDNNKAVLHYPGGEFEMDIIEASEGNNGVVLGKMLSETGLITFDPGYVSTGS
CCCCCEEEECCCCEEEECCCCCEEEEEEEECCCCCCEEEEEEHHHCCEEEECCCCCCCCC
TESKITYIDGDAGILRYRGYDIADLAENATFNEVSYLLINGELPTPDELHKFNDEIRHHT
CCCEEEEEECCCCEEEECCCCHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHH
LLDEDFKSQFNVFPRDAHPMATLASSVNILSTYYQDQLNPLDEAQLDKATVRLMAKVPML
HCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
AAYAHRARKGAPYMYPDNSLNARENFLRMMFGYPTEPYEIDPIMVKALDKLLILHADHEQ
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHEEEECCCCC
NCSTSTVRMIGSAQANMFVSIAGGINALSGPLHGGANQAVLEMLEDIKSNHGGDATEFMN
CCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHH
KVKNKEDGVRLMGFGHRVYKNYDPRAAIVKETAHEILEHLGGDDLLDLAIKLEEIALADD
HHCCCCCCEEEEECCHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
YFISRKLYPNVDFYTGLIYRAMGFPTDFFTVLFAIGRLPGWIAHYREQLGAAGNKINRPR
HHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCC
QVYTGNESRKLVPREER
EEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7522844; 12948626