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The map label for this gene is pdxR

Identifier: 62389676

GI number: 62389676

Start: 831097

End: 832458

Strand: Reverse

Name: pdxR

Synonym: cg0897

Alternate gene names: 62389676

Gene position: 832458-831097 (Counterclockwise)

Preceding gene: 62389679

Following gene: 62389674

Centisome position: 25.36

GC content: 64.46

Gene sequence:

>1362_bases
ATGCTTGCCGACCTTCCCATCGCCTTAAACCCACACGAACCAACATCCATCCCCACGCAGCTCACAGAACAGATCCGTCG
TCTCGTGGCGAGGGGAATTCTCACCCCAGGAGACCCGCTTCCCAGCAGTCGCTCACTATCCACCCAATTGGGGGTATCCC
GCGGCAGTGTGGTGACCGCTTATGACCAATTGGCCGGTGAAGGCTACCTCAGCACCGCCCGCGGTTCCGGTACAACGATC
AACCCAGATCTGCATTTGTTGAAGCCTGTGGAAATTGAGAAGAAGGAGACGTCGAGAAGCGTCCCGCCCCCGCTGCTCAA
CCTGAGCCCCGGCGTGCCCGATACCGCGACGCTCGCCGATTCCGCATGGCGCGCTGCGTGGCGCGAAGCCTGCGCCAAGC
CACCCACGCACTCCCCTGAGCAGGGACTTTTGAGGCTGCGGATCGAGATCGCCGACCACCTGCGCCAGATGCGTGGCCTC
ATGGTCGAGCCGGAGCAGATCATCGTCACCGCCGGCGCGCGCGAGGGGCTGAGTCTGCTGCTGCGCACCATGGATGCGCC
TGCCCGCATCGGCGTCGAATCGCCCGGCTACCCCAGCCTGCGCCGCATCCCGCAGGTGCTTGGCCATGAGACGATCGATG
TGCCGACCGACGAATCCGGCCTCGTACCCCGCGCGCTGCCCCACGACCTCAACGCGCTACTGGTAACCCCTAGCCATCAA
TATCCCTACGGCGGCTCGCTGCCCGCCGATCGCCGCACCGCGCTAGTCGCGTGGGCTGAGGCAAACGATGCGTTGCTTAT
TGAAGACGACTTCGATTCTGAGCTGCGCTACGTCGGTATGCCGCTTCCGCCGCTGCGTGCGCTGGCGCCCGATCGCACGA
TTCTGCTCGGCACGTTTTCCTCCGTGATCACACCACAAGTCGCCTGCGGATACCTCATCGCGCCGACGCCCCAGGCGCGC
GTGCTCGCCACGCTTCGCGGGATTCTCGGCCAGCCAGTCGGCGCCATCACCCAACACGCGCTCGCGTCCTACCTCGCCTC
AGGCGCTTTACGACGCCGCACCCAACGTTTGCGGCGCCTTTACCGACACCGCCGCTCCATCGTCCAAGACACCCTCGGTG
ACCTCCCGAATACGCAGCTTCGCCCCATCAACGGTGGCCTCCACGCAGTTCTCCTTTGCGACAAACCCCAAGACCTCGTC
GTCACCACACTCGCCTCCCGAGGCCTTAACGTCACCGCGCTTTCCCACTACTGGGGCGGCACCGGCGCAGACAACGGCAT
CGTCTTCGGCTTCGGCTCCCACGACGAAGACACCCTCAGATGGGTGCTTGCTGAGATCAGCGATGCGGTGTCTCTAGGCT
AA

Upstream 100 bases:

>100_bases
GTTTCGGTCATGACAGATGATCCAACGCCACAAAGTGGACTAGCGGTAGATCCACTTTCAGTCACTTGCATTAGACCACT
TTTTTGAGGACGATGAAGCC

Downstream 100 bases:

>100_bases
AGAAAAAACAGCCCGAGAGGAACGATCCTCTCGGGCTGTTTACTCAAACGCTACTGTGCGGACTGGTAGCTATCGACGGC
ACGATCAAGCGCATCGAGTG

Product: pyridoxine biosynthesis transcriptional regulator, aminotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MLADLPIALNPHEPTSIPTQLTEQIRRLVARGILTPGDPLPSSRSLSTQLGVSRGSVVTAYDQLAGEGYLSTARGSGTTI
NPDLHLLKPVEIEKKETSRSVPPPLLNLSPGVPDTATLADSAWRAAWREACAKPPTHSPEQGLLRLRIEIADHLRQMRGL
MVEPEQIIVTAGAREGLSLLLRTMDAPARIGVESPGYPSLRRIPQVLGHETIDVPTDESGLVPRALPHDLNALLVTPSHQ
YPYGGSLPADRRTALVAWAEANDALLIEDDFDSELRYVGMPLPPLRALAPDRTILLGTFSSVITPQVACGYLIAPTPQAR
VLATLRGILGQPVGAITQHALASYLASGALRRRTQRLRRLYRHRRSIVQDTLGDLPNTQLRPINGGLHAVLLCDKPQDLV
VTTLASRGLNVTALSHYWGGTGADNGIVFGFGSHDEDTLRWVLAEISDAVSLG

Sequences:

>Translated_453_residues
MLADLPIALNPHEPTSIPTQLTEQIRRLVARGILTPGDPLPSSRSLSTQLGVSRGSVVTAYDQLAGEGYLSTARGSGTTI
NPDLHLLKPVEIEKKETSRSVPPPLLNLSPGVPDTATLADSAWRAAWREACAKPPTHSPEQGLLRLRIEIADHLRQMRGL
MVEPEQIIVTAGAREGLSLLLRTMDAPARIGVESPGYPSLRRIPQVLGHETIDVPTDESGLVPRALPHDLNALLVTPSHQ
YPYGGSLPADRRTALVAWAEANDALLIEDDFDSELRYVGMPLPPLRALAPDRTILLGTFSSVITPQVACGYLIAPTPQAR
VLATLRGILGQPVGAITQHALASYLASGALRRRTQRLRRLYRHRRSIVQDTLGDLPNTQLRPINGGLHAVLLCDKPQDLV
VTTLASRGLNVTALSHYWGGTGADNGIVFGFGSHDEDTLRWVLAEISDAVSLG
>Mature_453_residues
MLADLPIALNPHEPTSIPTQLTEQIRRLVARGILTPGDPLPSSRSLSTQLGVSRGSVVTAYDQLAGEGYLSTARGSGTTI
NPDLHLLKPVEIEKKETSRSVPPPLLNLSPGVPDTATLADSAWRAAWREACAKPPTHSPEQGLLRLRIEIADHLRQMRGL
MVEPEQIIVTAGAREGLSLLLRTMDAPARIGVESPGYPSLRRIPQVLGHETIDVPTDESGLVPRALPHDLNALLVTPSHQ
YPYGGSLPADRRTALVAWAEANDALLIEDDFDSELRYVGMPLPPLRALAPDRTILLGTFSSVITPQVACGYLIAPTPQAR
VLATLRGILGQPVGAITQHALASYLASGALRRRTQRLRRLYRHRRSIVQDTLGDLPNTQLRPINGGLHAVLLCDKPQDLV
VTTLASRGLNVTALSHYWGGTGADNGIVFGFGSHDEDTLRWVLAEISDAVSLG

Specific function: May have a regulatory function in pyridoxine biosynthesis. Is said to also have an aminotransferase activity in valine biosynthesis as a double inactivation of ilvE and pdxR results in an auxotrophic requirement for valine

COG id: COG1167

COG function: function code KE; Transcriptional regulators containing a DNA-binding HTH domain and an aminotransferase domain (MocR family) and their eukaryotic orthologs

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1790797, Length=463, Percent_Identity=25.9179265658747, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787710, Length=454, Percent_Identity=26.8722466960352, Blast_Score=90, Evalue=3e-19,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): PDXR_CORGL (Q8NS92)

Other databases:

- EMBL:   AY238321
- EMBL:   BA000036
- EMBL:   BX927150
- RefSeq:   NP_600015.1
- RefSeq:   YP_225078.1
- ProteinModelPortal:   Q8NS92
- SMR:   Q8NS92
- GeneID:   1018782
- GeneID:   3345549
- GenomeReviews:   BA000036_GR
- GenomeReviews:   BX927147_GR
- KEGG:   cgb:cg0897
- KEGG:   cgl:NCgl0753
- HOGENOM:   HBG752297
- OMA:   YERHVRR
- ProtClustDB:   CLSK633034
- BioCyc:   CGLU196627:CG0897-MONOMER
- GO:   GO:0005622
- InterPro:   IPR000524
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR011991
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00035
- SMART:   SM00345

Pfam domain/function: PF00392 GntR; SSF53383 PyrdxlP-dep_Trfase_major

EC number: NA

Molecular weight: Translated: 48860; Mature: 48860

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLADLPIALNPHEPTSIPTQLTEQIRRLVARGILTPGDPLPSSRSLSTQLGVSRGSVVTA
CCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCEEEE
YDQLAGEGYLSTARGSGTTINPDLHLLKPVEIEKKETSRSVPPPLLNLSPGVPDTATLAD
HHHHCCCCCEEECCCCCCEECCCCEEECCCCCCHHHHCCCCCCCCCCCCCCCCCHHHHHH
SAWRAAWREACAKPPTHSPEQGLLRLRIEIADHLRQMRGLMVEPEQIIVTAGAREGLSLL
HHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHCCEECCCEEEEECCCHHHHHHH
LRTMDAPARIGVESPGYPSLRRIPQVLGHETIDVPTDESGLVPRALPHDLNALLVTPSHQ
HHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCC
YPYGGSLPADRRTALVAWAEANDALLIEDDFDSELRYVGMPLPPLRALAPDRTILLGTFS
CCCCCCCCCCCCEEEEEEECCCCEEEEECCCCCCCEEECCCCCCHHHCCCCCEEEEECHH
SVITPQVACGYLIAPTPQARVLATLRGILGQPVGAITQHALASYLASGALRRRTQRLRRL
HHHCHHHHCCEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YRHRRSIVQDTLGDLPNTQLRPINGGLHAVLLCDKPQDLVVTTLASRGLNVTALSHYWGG
HHHHHHHHHHHHHCCCCCCEEEECCCEEEEEEECCCCCHHHHHHHHCCCCEEEECCCCCC
TGADNGIVFGFGSHDEDTLRWVLAEISDAVSLG
CCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLADLPIALNPHEPTSIPTQLTEQIRRLVARGILTPGDPLPSSRSLSTQLGVSRGSVVTA
CCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCEEEE
YDQLAGEGYLSTARGSGTTINPDLHLLKPVEIEKKETSRSVPPPLLNLSPGVPDTATLAD
HHHHCCCCCEEECCCCCCEECCCCEEECCCCCCHHHHCCCCCCCCCCCCCCCCCHHHHHH
SAWRAAWREACAKPPTHSPEQGLLRLRIEIADHLRQMRGLMVEPEQIIVTAGAREGLSLL
HHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHCCEECCCEEEEECCCHHHHHHH
LRTMDAPARIGVESPGYPSLRRIPQVLGHETIDVPTDESGLVPRALPHDLNALLVTPSHQ
HHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCC
YPYGGSLPADRRTALVAWAEANDALLIEDDFDSELRYVGMPLPPLRALAPDRTILLGTFS
CCCCCCCCCCCCEEEEEEECCCCEEEEECCCCCCCEEECCCCCCHHHCCCCCEEEEECHH
SVITPQVACGYLIAPTPQARVLATLRGILGQPVGAITQHALASYLASGALRRRTQRLRRL
HHHCHHHHCCEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YRHRRSIVQDTLGDLPNTQLRPINGGLHAVLLCDKPQDLVVTTLASRGLNVTALSHYWGG
HHHHHHHHHHHHHCCCCCCEEEECCCEEEEEEECCCCCHHHHHHHHCCCCEEEECCCCCC
TGADNGIVFGFGSHDEDTLRWVLAEISDAVSLG
CCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12948641; 12948626