Click here to switch to the map view.

The map label for this gene is truA [H]

Identifier: 62389456

GI number: 62389456

Start: 577044

End: 577928

Strand: Direct

Name: truA [H]

Synonym: cg0657

Alternate gene names: 62389456

Gene position: 577044-577928 (Clockwise)

Preceding gene: 62389455

Following gene: 62389457

Centisome position: 17.58

GC content: 57.06

Gene sequence:

>885_bases
ATGGACAATTCAACGGTGCGAATCCGGCTGGATCTAGCGTATGACGGCACGGATTTTCATGGCTGGGCGAAGCAGGGGAC
CAGCGATCTACGCACCGTGCAAAAAGTGTTGGAAGACAATTTGAGCATGGTGCTGCGTGAGACTGTTGAATTGACTGTGG
CCGGGCGAACCGATGCGGGGGTGCATGCGGCGGGCCAGGTGGCGCACTTTGATATTCCGGCACACGCTTTAGAGCAGCGC
AGTATTGATGGCGATCCAAGCAAGTTGGTTCGGCGCTTGGGTCGGTTGCTGCCCGATGATATTCGGGTGCATGGCGTACG
TTTTGCCGAGCCCGGGTTTGATGCGCGATTTTCCGCGATGCGCAGGCACTACGTTTATCGCATTACGACGCATCCCGCCG
GCGCGCTGCCTACGCGCCGCCACGACACGGCGCAGTGGCCAAAACCTGTCGAACTAGAGCGGATGCAATTAGCCGCCGAT
GCACTGCTGGGGCTGCATGATTTTGTGGCGTTTTGCAAAGCTAAGCCACATGCGACGACGGTGCGTGAACTACAAAAATT
TGCGTGGAAAGACGTCTCCACTGACATCGAACCGCAGGTGTATGAAGCACACGTGGTGGCCGATGCTTTTTGCTGGTCGA
TGGTGCGCTCGCTGGTCGGCTCCTGCATGGCCGTGGGCGAAGGACGCCGCGGATCAGGGTTTACTGCAGAATTGCTTGAT
GCAAGCGAACGCAGCCCCATGGTTCCAGTAGCACCTGCGAAAGGTTTGAGCTTGGTTGGCGTGGATTATCCTTCCGCTGA
TAAGTTACAGGAAAGAGCGCTGGAAACCCGAGCTGTTCGCGAGTTTCCGGACGCGTCCGCGAGCCTAAAACTAGATGATG
AGTAA

Upstream 100 bases:

>100_bases
TTTACTGCGGGCATTTTACGTATCTGCACCCCGCCCGGCTGCGCTGAGCAGCCGTAAAGCGTGGGGCGTGACGTCGAAAA
GCAAAAAATGAAAGGCAGAC

Downstream 100 bases:

>100_bases
AAGGGACTAAACTCGTCTCTCGTATCTTTCAGTCATTTATGTCTGATAAGCAGGCGCGTGCGCCACACAATTGGCACAAT
CACAAGAAAGTGAAGTGGGA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MDNSTVRIRLDLAYDGTDFHGWAKQGTSDLRTVQKVLEDNLSMVLRETVELTVAGRTDAGVHAAGQVAHFDIPAHALEQR
SIDGDPSKLVRRLGRLLPDDIRVHGVRFAEPGFDARFSAMRRHYVYRITTHPAGALPTRRHDTAQWPKPVELERMQLAAD
ALLGLHDFVAFCKAKPHATTVRELQKFAWKDVSTDIEPQVYEAHVVADAFCWSMVRSLVGSCMAVGEGRRGSGFTAELLD
ASERSPMVPVAPAKGLSLVGVDYPSADKLQERALETRAVREFPDASASLKLDDE

Sequences:

>Translated_294_residues
MDNSTVRIRLDLAYDGTDFHGWAKQGTSDLRTVQKVLEDNLSMVLRETVELTVAGRTDAGVHAAGQVAHFDIPAHALEQR
SIDGDPSKLVRRLGRLLPDDIRVHGVRFAEPGFDARFSAMRRHYVYRITTHPAGALPTRRHDTAQWPKPVELERMQLAAD
ALLGLHDFVAFCKAKPHATTVRELQKFAWKDVSTDIEPQVYEAHVVADAFCWSMVRSLVGSCMAVGEGRRGSGFTAELLD
ASERSPMVPVAPAKGLSLVGVDYPSADKLQERALETRAVREFPDASASLKLDDE
>Mature_294_residues
MDNSTVRIRLDLAYDGTDFHGWAKQGTSDLRTVQKVLEDNLSMVLRETVELTVAGRTDAGVHAAGQVAHFDIPAHALEQR
SIDGDPSKLVRRLGRLLPDDIRVHGVRFAEPGFDARFSAMRRHYVYRITTHPAGALPTRRHDTAQWPKPVELERMQLAAD
ALLGLHDFVAFCKAKPHATTVRELQKFAWKDVSTDIEPQVYEAHVVADAFCWSMVRSLVGSCMAVGEGRRGSGFTAELLD
ASERSPMVPVAPAKGLSLVGVDYPSADKLQERALETRAVREFPDASASLKLDDE

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=284, Percent_Identity=32.0422535211268, Blast_Score=82, Evalue=4e-16,
Organism=Escherichia coli, GI1788657, Length=265, Percent_Identity=36.9811320754717, Blast_Score=137, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI71985046, Length=283, Percent_Identity=24.7349823321555, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321112, Length=310, Percent_Identity=29.3548387096774, Blast_Score=96, Evalue=5e-21,
Organism=Drosophila melanogaster, GI19922728, Length=306, Percent_Identity=26.4705882352941, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI116007752, Length=311, Percent_Identity=26.0450160771704, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 32509; Mature: 32509

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNSTVRIRLDLAYDGTDFHGWAKQGTSDLRTVQKVLEDNLSMVLRETVELTVAGRTDAG
CCCCEEEEEEEEEECCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCC
VHAAGQVAHFDIPAHALEQRSIDGDPSKLVRRLGRLLPDDIRVHGVRFAEPGFDARFSAM
CCCCCCEEEECCCHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHH
RRHYVYRITTHPAGALPTRRHDTAQWPKPVELERMQLAADALLGLHDFVAFCKAKPHATT
HHCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
VRELQKFAWKDVSTDIEPQVYEAHVVADAFCWSMVRSLVGSCMAVGEGRRGSGFTAELLD
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHC
ASERSPMVPVAPAKGLSLVGVDYPSADKLQERALETRAVREFPDASASLKLDDE
CCCCCCCCEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCC
>Mature Secondary Structure
MDNSTVRIRLDLAYDGTDFHGWAKQGTSDLRTVQKVLEDNLSMVLRETVELTVAGRTDAG
CCCCEEEEEEEEEECCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCC
VHAAGQVAHFDIPAHALEQRSIDGDPSKLVRRLGRLLPDDIRVHGVRFAEPGFDARFSAM
CCCCCCEEEECCCHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHH
RRHYVYRITTHPAGALPTRRHDTAQWPKPVELERMQLAADALLGLHDFVAFCKAKPHATT
HHCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
VRELQKFAWKDVSTDIEPQVYEAHVVADAFCWSMVRSLVGSCMAVGEGRRGSGFTAELLD
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHC
ASERSPMVPVAPAKGLSLVGVDYPSADKLQERALETRAVREFPDASASLKLDDE
CCCCCCCCEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12948626 [H]