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The map label for this gene is nth [H]

Identifier: 62389190

GI number: 62389190

Start: 307955

End: 308737

Strand: Direct

Name: nth [H]

Synonym: cg0353

Alternate gene names: 62389190

Gene position: 307955-308737 (Clockwise)

Preceding gene: 62389187

Following gene: 62389191

Centisome position: 9.38

GC content: 55.43

Gene sequence:

>783_bases
ATGGGTTCCATTACTCCGCAGAAGCGGCCTCGCGTGGGGTCTCACATCGCGAACAAGGGTCAAGAGACTGATATCGGGCG
AAAACGCCGAGCTCGACGCATCAATCGCACACTCACCGTGGCATATCCGGATGCGCACTGCGAATTAGATTTCACCAATC
CGCTAGAACTCACGGTCGCCACCATTTTGTCCGCCCAGTGCACGGACGTTCGCGTGAACCAGGTGACGCCCGCGTTGTTC
AAGCGCTATCCGACGGCCACAGATTACGCCAACGCCGATCGCACGGAATTGGAGGAGTTCATCCGTCCGACAGGCTTTTA
CCGCAACAAGGCCACTTCTTTAATCGGCCTGGGTGAGGCACTAATTTCGCTTCACGACGGCCAGGTCCCCGGTACCCTTG
AGCAGCTAGTTGAGCTGCCGGGGGTCGGGCGGAAAACCGCCAACGTGGTGCTGGGAAATGCTTTCGGTGTTCCGGGAATC
ACGGTGGATACACACTTTGGCAGGTTGGTGCGTCGCCTGAAGCTCACTGATGAAGAAGATCCCGTCAAGGTGGAAAAAGT
GATGAACGAACTCATCGAAAAGCCTGAGTGGACCATGTTTTCACATAGGCTGATCTTCCACGGACGTAGGATATGTCATA
GTCGACGCGCCGCCTGTGGAGCCTGCATGCTGGCAGCTGATTGCCCATCCTTTGGTTTGGAGGGGCCGTCAGATCCATTT
GAGGCGCAAAAACTCATTAAAAGTGATGATAGGGAGCACCTGCTGAAAATGGCAGGAATGTAG

Upstream 100 bases:

>100_bases
CAAATGCAAAAAGCATCAGCAAGAGCGGGATGATCACCACAAGCAAACCTGTCATGGGAGTCATTCTAATGATGAATCAA
ATCCGCGCTAACTTAAGGGT

Downstream 100 bases:

>100_bases
AAAACAAATGACAAGCAGTGCAAAGTGGTCCATCGTTGGAGTTGTCGCCATCCTGGCTGTGATCGTTGCGTTAATCCCGC
AGCTTGTGGGTGGAGAAAGC

Product: endonuclease III protein

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MGSITPQKRPRVGSHIANKGQETDIGRKRRARRINRTLTVAYPDAHCELDFTNPLELTVATILSAQCTDVRVNQVTPALF
KRYPTATDYANADRTELEEFIRPTGFYRNKATSLIGLGEALISLHDGQVPGTLEQLVELPGVGRKTANVVLGNAFGVPGI
TVDTHFGRLVRRLKLTDEEDPVKVEKVMNELIEKPEWTMFSHRLIFHGRRICHSRRAACGACMLAADCPSFGLEGPSDPF
EAQKLIKSDDREHLLKMAGM

Sequences:

>Translated_260_residues
MGSITPQKRPRVGSHIANKGQETDIGRKRRARRINRTLTVAYPDAHCELDFTNPLELTVATILSAQCTDVRVNQVTPALF
KRYPTATDYANADRTELEEFIRPTGFYRNKATSLIGLGEALISLHDGQVPGTLEQLVELPGVGRKTANVVLGNAFGVPGI
TVDTHFGRLVRRLKLTDEEDPVKVEKVMNELIEKPEWTMFSHRLIFHGRRICHSRRAACGACMLAADCPSFGLEGPSDPF
EAQKLIKSDDREHLLKMAGM
>Mature_259_residues
GSITPQKRPRVGSHIANKGQETDIGRKRRARRINRTLTVAYPDAHCELDFTNPLELTVATILSAQCTDVRVNQVTPALFK
RYPTATDYANADRTELEEFIRPTGFYRNKATSLIGLGEALISLHDGQVPGTLEQLVELPGVGRKTANVVLGNAFGVPGIT
VDTHFGRLVRRLKLTDEEDPVKVEKVMNELIEKPEWTMFSHRLIFHGRRICHSRRAACGACMLAADCPSFGLEGPSDPFE
AQKLIKSDDREHLLKMAGM

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=193, Percent_Identity=29.0155440414508, Blast_Score=86, Evalue=3e-17,
Organism=Escherichia coli, GI1787920, Length=186, Percent_Identity=37.6344086021505, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1789331, Length=181, Percent_Identity=26.5193370165746, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17554540, Length=181, Percent_Identity=34.2541436464088, Blast_Score=98, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6324530, Length=219, Percent_Identity=25.5707762557078, Blast_Score=69, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6319304, Length=146, Percent_Identity=30.8219178082192, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45550361, Length=179, Percent_Identity=30.7262569832402, Blast_Score=87, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 28919; Mature: 28787

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSITPQKRPRVGSHIANKGQETDIGRKRRARRINRTLTVAYPDAHCELDFTNPLELTVA
CCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHCCEEEEEECCCEEEECCCCCHHHHHH
TILSAQCTDVRVNQVTPALFKRYPTATDYANADRTELEEFIRPTGFYRNKATSLIGLGEA
HHHHHHCCCEEHHHCCHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHH
LISLHDGQVPGTLEQLVELPGVGRKTANVVLGNAFGVPGITVDTHFGRLVRRLKLTDEED
HHHHCCCCCCHHHHHHHHCCCCCCCHHHEEEECCCCCCCCEEHHHHHHHHHHHHCCCCCC
PVKVEKVMNELIEKPEWTMFSHRLIFHGRRICHSRRAACGACMLAADCPSFGLEGPSDPF
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
EAQKLIKSDDREHLLKMAGM
HHHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure 
GSITPQKRPRVGSHIANKGQETDIGRKRRARRINRTLTVAYPDAHCELDFTNPLELTVA
CCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHCCEEEEEECCCEEEECCCCCHHHHHH
TILSAQCTDVRVNQVTPALFKRYPTATDYANADRTELEEFIRPTGFYRNKATSLIGLGEA
HHHHHHCCCEEHHHCCHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHH
LISLHDGQVPGTLEQLVELPGVGRKTANVVLGNAFGVPGITVDTHFGRLVRRLKLTDEED
HHHHCCCCCCHHHHHHHHCCCCCCCHHHEEEECCCCCCCCEEHHHHHHHHHHHHCCCCCC
PVKVEKVMNELIEKPEWTMFSHRLIFHGRRICHSRRAACGACMLAADCPSFGLEGPSDPF
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
EAQKLIKSDDREHLLKMAGM
HHHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]