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The map label for this gene is alkB [H]

Identifier: 62389038

GI number: 62389038

Start: 156849

End: 157541

Strand: Direct

Name: alkB [H]

Synonym: cg0181

Alternate gene names: 62389038

Gene position: 156849-157541 (Clockwise)

Preceding gene: 62389036

Following gene: 62389039

Centisome position: 4.78

GC content: 52.81

Gene sequence:

>693_bases
GTGCCTACGCTTTTCGACGATCCCCTCCACCAACTTCCCAGACCACCGCGCAGGGTTGCAGCTGGCGTGGTGCACCTGCC
CAACTTCCTCGGCTTGGCCGAGCAAAAGGCACTGGTCGCCCAGGCTCGAGACCTTGCGCGTGAAGTTGTGGGCACACCGT
TGGCGATGGTCCGACCCAAGCTGAAAAGTGGTCAGATGAGTGTCCATATGTTGCATTTAGGAAAGTACTGGGCATCCAAT
CCTTATCGCTATGTGGATGTCGTTGATGGTTTTCCAGTGCCACCACTGCCGGATTCTTTCGTGGACTTGGCACACCGAGC
ATTGCTGTCTGCAGGATCTTTAAGCAATTCACTTCAGTCTTGGTCGGAGGCATATCGAGCAGAAGCGGCATTGGTGAATT
ACTACTCGCCAGATGCTTCAATGGGCATGCACCAAGACGCTAATGAAGAATCGGAAGCCCCTGTGATCTCCCTATCGATC
GGTGATACCGGAATCTTTCGACTGGGCGGCACTCTAAACCGCAATAAACCGTGGACAGATATCCCACTAATGAGTGGTGA
TCTCATCGTTTTCGGAGGTGCAAACAGGCAAGCATTTCATGGCATTCCCTCTATTGAGGCCAATACTGCACCTGCAGGAT
GCGGGTTGAAAGAAGGGCGAATCAACATCACGATCCGCCAACTTGCACTATAA

Upstream 100 bases:

>100_bases
TCACGTTCCGCGCCGGTAGCCACGTACCAATCGCCGCTGCTCATTTTCTCGATAGATTCAAAGTCATTGTCCATATCCTC
CATTGTGCAGGAGAACTTTA

Downstream 100 bases:

>100_bases
AACTCCAGGTAGAGGTATGTCTCGGGGGTGAAATAGCCCCGCAATGTCCCATGCTCAAATTATGGTTCGCGCCATGACAA
CAAACAAAGCTAATCCAAAT

Product: DNA repair protein

Products: NA

Alternate protein names: Alkylated DNA repair protein AlkB [H]

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MPTLFDDPLHQLPRPPRRVAAGVVHLPNFLGLAEQKALVAQARDLAREVVGTPLAMVRPKLKSGQMSVHMLHLGKYWASN
PYRYVDVVDGFPVPPLPDSFVDLAHRALLSAGSLSNSLQSWSEAYRAEAALVNYYSPDASMGMHQDANEESEAPVISLSI
GDTGIFRLGGTLNRNKPWTDIPLMSGDLIVFGGANRQAFHGIPSIEANTAPAGCGLKEGRINITIRQLAL

Sequences:

>Translated_230_residues
MPTLFDDPLHQLPRPPRRVAAGVVHLPNFLGLAEQKALVAQARDLAREVVGTPLAMVRPKLKSGQMSVHMLHLGKYWASN
PYRYVDVVDGFPVPPLPDSFVDLAHRALLSAGSLSNSLQSWSEAYRAEAALVNYYSPDASMGMHQDANEESEAPVISLSI
GDTGIFRLGGTLNRNKPWTDIPLMSGDLIVFGGANRQAFHGIPSIEANTAPAGCGLKEGRINITIRQLAL
>Mature_229_residues
PTLFDDPLHQLPRPPRRVAAGVVHLPNFLGLAEQKALVAQARDLAREVVGTPLAMVRPKLKSGQMSVHMLHLGKYWASNP
YRYVDVVDGFPVPPLPDSFVDLAHRALLSAGSLSNSLQSWSEAYRAEAALVNYYSPDASMGMHQDANEESEAPVISLSIG
DTGIFRLGGTLNRNKPWTDIPLMSGDLIVFGGANRQAFHGIPSIEANTAPAGCGLKEGRINITIRQLAL

Specific function: Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable a

COG id: COG3145

COG function: function code L; Alkylated DNA repair protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain [H]

Homologues:

Organism=Homo sapiens, GI87298840, Length=95, Percent_Identity=38.9473684210526, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1788541, Length=217, Percent_Identity=34.1013824884793, Blast_Score=91, Evalue=6e-20,
Organism=Drosophila melanogaster, GI45555401, Length=93, Percent_Identity=38.7096774193548, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004574 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 24900; Mature: 24769

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTLFDDPLHQLPRPPRRVAAGVVHLPNFLGLAEQKALVAQARDLAREVVGTPLAMVRPK
CCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCC
LKSGQMSVHMLHLGKYWASNPYRYVDVVDGFPVPPLPDSFVDLAHRALLSAGSLSNSLQS
CCCCCEEEEEEEECHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
WSEAYRAEAALVNYYSPDASMGMHQDANEESEAPVISLSIGDTGIFRLGGTLNRNKPWTD
HHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCCCCCCC
IPLMSGDLIVFGGANRQAFHGIPSIEANTAPAGCGLKEGRINITIRQLAL
CCEECCCEEEEECCCCHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEEECC
>Mature Secondary Structure 
PTLFDDPLHQLPRPPRRVAAGVVHLPNFLGLAEQKALVAQARDLAREVVGTPLAMVRPK
CCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCC
LKSGQMSVHMLHLGKYWASNPYRYVDVVDGFPVPPLPDSFVDLAHRALLSAGSLSNSLQS
CCCCCEEEEEEEECHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
WSEAYRAEAALVNYYSPDASMGMHQDANEESEAPVISLSIGDTGIFRLGGTLNRNKPWTD
HHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCCCCCCC
IPLMSGDLIVFGGANRQAFHGIPSIEANTAPAGCGLKEGRINITIRQLAL
CCEECCCEEEEECCCCHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3536913; 9097040; 9278503; 3887409; 7928996 [H]