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The map label for this gene is bioB [H]

Identifier: 62388967

GI number: 62388967

Start: 74491

End: 75495

Strand: Direct

Name: bioB [H]

Synonym: cg0095

Alternate gene names: 62388967

Gene position: 74491-75495 (Clockwise)

Preceding gene: 62388966

Following gene: 62388968

Centisome position: 2.27

GC content: 54.63

Gene sequence:

>1005_bases
ATGACCATCCCCGGCACCATCCTTGACACCGCCCGCACCCAAGTTCTGGAACAGGGAATTGGCCTTAATCAGCAGCAGTT
GATGGAGGTTCTCACCTTGCCTGAAGAGCAAATCCCAGACTTGATGGAATTAGCCCACCAGGTTCGGTTGAAGTGGTGTG
GGGAAGAAATCGAGGTCGAGGGCATTATTTCCCTCAAAACTGGCGGTTGCCCTGAAGATTGTCATTTCTGCTCACAGTCT
GGGTTGTTTGAATCGCCGGTGCGTTCGGTGTGGCTGGATATTCCGAATCTGGTTGAAGCCGCTAAACAGACCGCAAAAAC
TGGCGCTACCGAATTCTGTATCGTCGCCGCAGTCAAGGGGCCTGATGAGAGGCTCATGACCCAGCTGGAGGAAGCAGTCC
TCGCGATTCACTCTGAAGTTGAAATTGAAGTCGCAGCATCGATCGGAACGTTAAATAAGGAACAGGTGGATCGCCTCGCT
GCTGCCGGCGTGCACCGCTACAACCATAATTTGGAAACTGCGCGTTCCTATTTCCCTGAAGTTGTCACCACTCATACATG
GGAAGAGCGCCGCGAAACTTTGCGCCTGGTGGCAGAAGCTGGAATGGAAGTCTGTTCCGGCGGAATCTTAGGAATGGGCG
AAACTTTAGAGCAGCGCGCCGAGTTTGCCGTGCAGCTGGCGGAGCTTGATCCGCACGAAGTCCCCATGAACTTCCTTGAT
CCTCGCCCGGGCACCCCATTTGCCGATAGGGAATTGATGGACAGCCGTGACGCTCTGCGCTCTATTGGTGCGTTCCGCCT
TGCGATGCCTCACACCATGCTTCGTTTTGCTGGCGGTCGCGAGCTGACTTTGGGCGACAAGGGTTCCGAGCAAGCCCTCC
TGGGAGGCATCAATGCGATGATCGTCGGAAACTACCTGACTACGCTCGGCCGCCCAATGGAAGATGACCTCGACATGATG
GATCGTCTCCAGCTGCCCATCAAAGTCCTTAATAAGGTCATCTAA

Upstream 100 bases:

>100_bases
GTTTAAGGCACGTGTCATTTTGCATGCGCGTGCCCAATTCTTCTTAAGTGTGCTCAATTTTTAAGCGCGCAGTTATTGAC
AACCAGCCTCTAGGAGATCC

Downstream 100 bases:

>100_bases
GAAGCACGCGCATGAACGACGATCTCCAAATGGCCATGATTCTGGCAGACACCCCGATCTTTCATCCAAACACCGGCAAG
CCCTTCGATGGTGAACATAA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MTIPGTILDTARTQVLEQGIGLNQQQLMEVLTLPEEQIPDLMELAHQVRLKWCGEEIEVEGIISLKTGGCPEDCHFCSQS
GLFESPVRSVWLDIPNLVEAAKQTAKTGATEFCIVAAVKGPDERLMTQLEEAVLAIHSEVEIEVAASIGTLNKEQVDRLA
AAGVHRYNHNLETARSYFPEVVTTHTWEERRETLRLVAEAGMEVCSGGILGMGETLEQRAEFAVQLAELDPHEVPMNFLD
PRPGTPFADRELMDSRDALRSIGAFRLAMPHTMLRFAGGRELTLGDKGSEQALLGGINAMIVGNYLTTLGRPMEDDLDMM
DRLQLPIKVLNKVI

Sequences:

>Translated_334_residues
MTIPGTILDTARTQVLEQGIGLNQQQLMEVLTLPEEQIPDLMELAHQVRLKWCGEEIEVEGIISLKTGGCPEDCHFCSQS
GLFESPVRSVWLDIPNLVEAAKQTAKTGATEFCIVAAVKGPDERLMTQLEEAVLAIHSEVEIEVAASIGTLNKEQVDRLA
AAGVHRYNHNLETARSYFPEVVTTHTWEERRETLRLVAEAGMEVCSGGILGMGETLEQRAEFAVQLAELDPHEVPMNFLD
PRPGTPFADRELMDSRDALRSIGAFRLAMPHTMLRFAGGRELTLGDKGSEQALLGGINAMIVGNYLTTLGRPMEDDLDMM
DRLQLPIKVLNKVI
>Mature_333_residues
TIPGTILDTARTQVLEQGIGLNQQQLMEVLTLPEEQIPDLMELAHQVRLKWCGEEIEVEGIISLKTGGCPEDCHFCSQSG
LFESPVRSVWLDIPNLVEAAKQTAKTGATEFCIVAAVKGPDERLMTQLEEAVLAIHSEVEIEVAASIGTLNKEQVDRLAA
AGVHRYNHNLETARSYFPEVVTTHTWEERRETLRLVAEAGMEVCSGGILGMGETLEQRAEFAVQLAELDPHEVPMNFLDP
RPGTPFADRELMDSRDALRSIGAFRLAMPHTMLRFAGGRELTLGDKGSEQALLGGINAMIVGNYLTTLGRPMEDDLDMMD
RLQLPIKVLNKVI

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=304, Percent_Identity=37.828947368421, Blast_Score=208, Evalue=3e-55,
Organism=Saccharomyces cerevisiae, GI6321725, Length=319, Percent_Identity=29.7805642633229, Blast_Score=162, Evalue=5e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 36857; Mature: 36726

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIPGTILDTARTQVLEQGIGLNQQQLMEVLTLPEEQIPDLMELAHQVRLKWCGEEIEVE
CCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHCCCHHHHHHHHHHHHHCCCEEEEE
GIISLKTGGCPEDCHFCSQSGLFESPVRSVWLDIPNLVEAAKQTAKTGATEFCIVAAVKG
EEEEECCCCCCHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEEECC
PDERLMTQLEEAVLAIHSEVEIEVAASIGTLNKEQVDRLAAAGVHRYNHNLETARSYFPE
CHHHHHHHHHHHHHHHHHHCEEEEEHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCH
VVTTHTWEERRETLRLVAEAGMEVCSGGILGMGETLEQRAEFAVQLAELDPHEVPMNFLD
HHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
PRPGTPFADRELMDSRDALRSIGAFRLAMPHTMLRFAGGRELTLGDKGSEQALLGGINAM
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHCHHHH
IVGNYLTTLGRPMEDDLDMMDRLQLPIKVLNKVI
HHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHCC
>Mature Secondary Structure 
TIPGTILDTARTQVLEQGIGLNQQQLMEVLTLPEEQIPDLMELAHQVRLKWCGEEIEVE
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHCCCHHHHHHHHHHHHHCCCEEEEE
GIISLKTGGCPEDCHFCSQSGLFESPVRSVWLDIPNLVEAAKQTAKTGATEFCIVAAVKG
EEEEECCCCCCHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEEECC
PDERLMTQLEEAVLAIHSEVEIEVAASIGTLNKEQVDRLAAAGVHRYNHNLETARSYFPE
CHHHHHHHHHHHHHHHHHHCEEEEEHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCH
VVTTHTWEERRETLRLVAEAGMEVCSGGILGMGETLEQRAEFAVQLAELDPHEVPMNFLD
HHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
PRPGTPFADRELMDSRDALRSIGAFRLAMPHTMLRFAGGRELTLGDKGSEQALLGGINAM
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHCHHHH
IVGNYLTTLGRPMEDDLDMMDRLQLPIKVLNKVI
HHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA