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The map label for this gene is citA [H]

Identifier: 62388963

GI number: 62388963

Start: 70507

End: 72162

Strand: Direct

Name: citA [H]

Synonym: cg0089

Alternate gene names: 62388963

Gene position: 70507-72162 (Clockwise)

Preceding gene: 62388960

Following gene: 62388964

Centisome position: 2.15

GC content: 53.99

Gene sequence:

>1656_bases
ATGTCTGTTGGTGGATCCGACTGGAAAAACTTCAAGGAGGTGGACATCATTCGCTTTGCTACCCGAATACTGGTGATTCA
AGTGGCTACCGTCGCGTTGGTGGTAGCTATTTGCACCGGAATTTTCGCAGTTTTGATGATGGATCAGATGAAAACTGAGG
CCGAGCACACAGCGCTGTCCATCGGACGTTCGGTGGCATCCAACCCGCAGATCCGCGAGGAAGTAGCGCTTGATACTCAA
ACAGGAGCAAACCCATCGGCCGAAGAATTAGCCGATGGAGATATCCAAGCGGTTGCACAGGCGGCCAATGAACGCACTGG
AGCTTTGTTTGTCGTTATCACTGACGGTTTAGGTATCCGCCTGTCCCACCCAGATGAGGAACGTCTGGGGGAGCAGGTGA
GCACTAGCTTTGAGGCTGCCATGCGGGGTGAAGAAACCATGGCGTGGGAGACTGGGACCCTCGGTGCGTCCGCGCGAGCA
AAAGTGCCTATCTTTGCGCCGGATTCTAGTGTTCCAGTCGGTGAGGTCAGTGTTGGGTTTGAGCGAGACAGTGTGTATTC
CCGCCTGCCCATGTTCCTCGCCGCCCTTGCTCTTATTTCTGTGTTGGGAATCCTTATCGGCGTGGGTGTAGCCATGGGCA
TGCGACGCCGTTGGGAACGCGTGACCTTGGGTTTGCAGCCGGAGGAGCTAGTGACCCTTGTGCAAAATCAGACTGCAGTC
ATCGATGGCATTGATGAGGGCGTGCTGGCGCTGAGCCCAAACGGAACAATTGGGGTGCATAATGAGCAGGCGCAATCCAT
GATTGGTGCAGGTCCTATGAGTGGCAGGACGTTGAAAGAACTAGGGCTTGACCTGGGTCTTGATGGCGTTGTATTGCATG
GTCAGCATCCGGAAACCGTTGCCCATAACGGCAGGATCCTCTATCTGGATTTCCACCCCGTGCGCCGTGGGGATCAAGAT
TTAGGCTACGTGGTAACCATCCGCGATCGTACCGACATCATTGAACTCAGTGAACGCCTCGACTCTGTGCGCACCATGAC
CCACGCACTCCGCGCCCAGCGCCACGAGTTTGCCAACCGCATCCACACCGCAACAGGGCTTATCGACGCCGGCCGCGTCC
ACGACGCGGCAGAGTTTCTAGGCGATATATCCCGCAACGGGGGACAGTCACATCCATTGATCGGATCAGCGCACCTCAAT
GAAGCATTTTTGAGCTCATTTTTAAGTACTGCTTCTATTTCGGCATCTGAAAAGGGCGTTAGTCTGCGCATCAACTCTGA
CACGCTCATCCTTGGCACTGTTAAAGATCCAGAAGATGTAGCAACCATTTTGGGTAATTTAATCAACAATGCCATCGACG
CCGCGGTGGCAGGTGAAGCCCCACGGTGGATTGAGCTTACGTTGATGGATGATGCCGATACGCTGGTCATTTCTGTTGCA
GATTCTGGTCCTGGAATCCCAGAGGGCGTGGATGTATTTGCCACAGCCACCCAGATAGGAGACTCTGAAGATAATGAACG
CACCCACGGGCATGGCATTGGTCTAAAACTGTGCCGGGCTTTGGCTAGATCACATGGTGGCGATGTCTGGGTGATTGATA
GAGGAACCGAAGATGGCGCTGTATTTGGAGTGAAACTACCGGGAGTAATGGAGTAA

Upstream 100 bases:

>100_bases
TGAGGTAGTGCGCAAAATAAGACTTTTGTGCATTATGATCAGAATTGTTGGCCTGGGACTTCGCTTCACGCTCTGCTGAT
AATCGCCCCCGGGGGTAGAC

Downstream 100 bases:

>100_bases
TGGATCAAACACTTAAAGTTTTAGTAATTGATGATGATTTCCGCGTCGCCGGCATTCACGCCTCCATCGTTGATGCGTCC
CCTGGATTTTCGGTGGTCGG

Product: two-component system sensory transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 551; Mature: 550

Protein sequence:

>551_residues
MSVGGSDWKNFKEVDIIRFATRILVIQVATVALVVAICTGIFAVLMMDQMKTEAEHTALSIGRSVASNPQIREEVALDTQ
TGANPSAEELADGDIQAVAQAANERTGALFVVITDGLGIRLSHPDEERLGEQVSTSFEAAMRGEETMAWETGTLGASARA
KVPIFAPDSSVPVGEVSVGFERDSVYSRLPMFLAALALISVLGILIGVGVAMGMRRRWERVTLGLQPEELVTLVQNQTAV
IDGIDEGVLALSPNGTIGVHNEQAQSMIGAGPMSGRTLKELGLDLGLDGVVLHGQHPETVAHNGRILYLDFHPVRRGDQD
LGYVVTIRDRTDIIELSERLDSVRTMTHALRAQRHEFANRIHTATGLIDAGRVHDAAEFLGDISRNGGQSHPLIGSAHLN
EAFLSSFLSTASISASEKGVSLRINSDTLILGTVKDPEDVATILGNLINNAIDAAVAGEAPRWIELTLMDDADTLVISVA
DSGPGIPEGVDVFATATQIGDSEDNERTHGHGIGLKLCRALARSHGGDVWVIDRGTEDGAVFGVKLPGVME

Sequences:

>Translated_551_residues
MSVGGSDWKNFKEVDIIRFATRILVIQVATVALVVAICTGIFAVLMMDQMKTEAEHTALSIGRSVASNPQIREEVALDTQ
TGANPSAEELADGDIQAVAQAANERTGALFVVITDGLGIRLSHPDEERLGEQVSTSFEAAMRGEETMAWETGTLGASARA
KVPIFAPDSSVPVGEVSVGFERDSVYSRLPMFLAALALISVLGILIGVGVAMGMRRRWERVTLGLQPEELVTLVQNQTAV
IDGIDEGVLALSPNGTIGVHNEQAQSMIGAGPMSGRTLKELGLDLGLDGVVLHGQHPETVAHNGRILYLDFHPVRRGDQD
LGYVVTIRDRTDIIELSERLDSVRTMTHALRAQRHEFANRIHTATGLIDAGRVHDAAEFLGDISRNGGQSHPLIGSAHLN
EAFLSSFLSTASISASEKGVSLRINSDTLILGTVKDPEDVATILGNLINNAIDAAVAGEAPRWIELTLMDDADTLVISVA
DSGPGIPEGVDVFATATQIGDSEDNERTHGHGIGLKLCRALARSHGGDVWVIDRGTEDGAVFGVKLPGVME
>Mature_550_residues
SVGGSDWKNFKEVDIIRFATRILVIQVATVALVVAICTGIFAVLMMDQMKTEAEHTALSIGRSVASNPQIREEVALDTQT
GANPSAEELADGDIQAVAQAANERTGALFVVITDGLGIRLSHPDEERLGEQVSTSFEAAMRGEETMAWETGTLGASARAK
VPIFAPDSSVPVGEVSVGFERDSVYSRLPMFLAALALISVLGILIGVGVAMGMRRRWERVTLGLQPEELVTLVQNQTAVI
DGIDEGVLALSPNGTIGVHNEQAQSMIGAGPMSGRTLKELGLDLGLDGVVLHGQHPETVAHNGRILYLDFHPVRRGDQDL
GYVVTIRDRTDIIELSERLDSVRTMTHALRAQRHEFANRIHTATGLIDAGRVHDAAEFLGDISRNGGQSHPLIGSAHLNE
AFLSSFLSTASISASEKGVSLRINSDTLILGTVKDPEDVATILGNLINNAIDAAVAGEAPRWIELTLMDDADTLVISVAD
SGPGIPEGVDVFATATQIGDSEDNERTHGHGIGLKLCRALARSHGGDVWVIDRGTEDGAVFGVKLPGVME

Specific function: Member of the two-component regulatory system CitT/CitS. Functions probably as a membrane-associated protein kinase that phosphorylates CitT in response to environmental citrate or Mg(2+)-citrate complex [H]

COG id: COG3290

COG function: function code T; Signal transduction histidine kinase regulating citrate/malate metabolism

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790567, Length=547, Percent_Identity=25.9597806215722, Blast_Score=162, Evalue=6e-41,
Organism=Escherichia coli, GI1786837, Length=558, Percent_Identity=27.0609318996416, Blast_Score=161, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR016120
- InterPro:   IPR005467
- InterPro:   IPR016121 [H]

Pfam domain/function: PF02518 HATPase_c; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 58609; Mature: 58478

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVGGSDWKNFKEVDIIRFATRILVIQVATVALVVAICTGIFAVLMMDQMKTEAEHTALS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGRSVASNPQIREEVALDTQTGANPSAEELADGDIQAVAQAANERTGALFVVITDGLGIR
HHHHHCCCCCHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHHCCCCCEEEEEEECCCEEE
LSHPDEERLGEQVSTSFEAAMRGEETMAWETGTLGASARAKVPIFAPDSSVPVGEVSVGF
ECCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEECCCCCCCCCCEEECC
ERDSVYSRLPMFLAALALISVLGILIGVGVAMGMRRRWERVTLGLQPEELVTLVQNQTAV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEE
IDGIDEGVLALSPNGTIGVHNEQAQSMIGAGPMSGRTLKELGLDLGLDGVVLHGQHPETV
EECCCCCEEEECCCCCEECCCHHHHHHHCCCCCCCCHHHHHCCCCCCCCEEEECCCCCHH
AHNGRILYLDFHPVRRGDQDLGYVVTIRDRTDIIELSERLDSVRTMTHALRAQRHEFANR
HCCCEEEEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IHTATGLIDAGRVHDAAEFLGDISRNGGQSHPLIGSAHLNEAFLSSFLSTASISASEKGV
HHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHHHHCCCCCCCCE
SLRINSDTLILGTVKDPEDVATILGNLINNAIDAAVAGEAPRWIELTLMDDADTLVISVA
EEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEEE
DSGPGIPEGVDVFATATQIGDSEDNERTHGHGIGLKLCRALARSHGGDVWVIDRGTEDGA
CCCCCCCCCCHHEEEHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCE
VFGVKLPGVME
EEEEECCCCCC
>Mature Secondary Structure 
SVGGSDWKNFKEVDIIRFATRILVIQVATVALVVAICTGIFAVLMMDQMKTEAEHTALS
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGRSVASNPQIREEVALDTQTGANPSAEELADGDIQAVAQAANERTGALFVVITDGLGIR
HHHHHCCCCCHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHHCCCCCEEEEEEECCCEEE
LSHPDEERLGEQVSTSFEAAMRGEETMAWETGTLGASARAKVPIFAPDSSVPVGEVSVGF
ECCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEECCCCCCCCCCEEECC
ERDSVYSRLPMFLAALALISVLGILIGVGVAMGMRRRWERVTLGLQPEELVTLVQNQTAV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEE
IDGIDEGVLALSPNGTIGVHNEQAQSMIGAGPMSGRTLKELGLDLGLDGVVLHGQHPETV
EECCCCCEEEECCCCCEECCCHHHHHHHCCCCCCCCHHHHHCCCCCCCCEEEECCCCCHH
AHNGRILYLDFHPVRRGDQDLGYVVTIRDRTDIIELSERLDSVRTMTHALRAQRHEFANR
HCCCEEEEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IHTATGLIDAGRVHDAAEFLGDISRNGGQSHPLIGSAHLNEAFLSSFLSTASISASEKGV
HHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHHHHCCCCCCCCE
SLRINSDTLILGTVKDPEDVATILGNLINNAIDAAVAGEAPRWIELTLMDDADTLVISVA
EEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEEE
DSGPGIPEGVDVFATATQIGDSEDNERTHGHGIGLKLCRALARSHGGDVWVIDRGTEDGA
CCCCCCCCCCHHEEEHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCE
VFGVKLPGVME
EEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10484179; 11058132 [H]