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The map label for this gene is sucB [H]

Identifier: 62185036

GI number: 62185036

Start: 467296

End: 468393

Strand: Direct

Name: sucB [H]

Synonym: CAB407

Alternate gene names: 62185036

Gene position: 467296-468393 (Clockwise)

Preceding gene: 62185035

Following gene: 62185043

Centisome position: 40.83

GC content: 41.8

Gene sequence:

>1098_bases
ATGATCACAGAAGTACGCATTCCAAATGTCGCTGAATCCATAAGCGAAGTCACAATAGCTTCTCTTTTGGTAACTTCAGA
GAGCTTGGTTCAGGAGAATCAAGGGATTATGGAGATCGAAAGCGAAAAGGTAAATCAACTGATCTATGCGCCTATATCTG
GAAGGATTGTTTGGTCTGTCGCTGAAGGAGATGTTGTTGCCGTCGGTGGAATTGTTGCTAGGATCTATGATGCTAATGAA
TCTGTTTCTGAAAGTACAGTCAAGGACACTCCCGTGGGGGAGACAGTAGATGCGGAGATCATTTGTTTCCCGAGGTCTAC
AGCTCACAATCCTCCTTCTGAGGGAAAGACATTTGTTCCTTTACGTGAGAAAATGCAAGAAGAACCGCAACGTTCGGGAG
CTAAAAATGAGGTGCGTGAGCGTATGTCATCAATACGCAAAACGATTTCTCGACGTTTAGTTACAGCTCTTCATGAATCC
GCAATGTTAACAACATTCAATGAGATTCATATGACCCCTCTGATGAAACTGCGGAAGGAAAAACAAGAGGCCTTTTCTGC
TCGTTATAATGTGAAACTGGGTTTAATGTCCTTTTTCATAAAAGCGGTTATTGAAGCGCTGAAGGCTTATCCTCGTGTCA
ATGCGTATATTGATGGTGATGAGATTGTCTATCGCCAGTATTACGATATTTCTATTGCTGTGGGAACAGAACGTGGTCTT
GTTGTTCCAGTGATTCGTGAGGCCGATAAGCTCTCAAGTGGTGACATTGAAATGAAGCTTGCGGATTTAGCGAGTAGAGC
TCGAGATGGGTTAATTTCTCTTCCAGAGTTAGAAGGAGGAAGTTTCACTATTACAAATGGTGGGGTTTACGGTTCCTTAC
TTTCTACACCTATTATCAATCCTCCTCAAGTAGGGATATTGGGAATGCACAAAATAGAAAAACGCCCTGTAGTGATTGAT
AATACCATCGCCATAGCAGATATGATGTATGTTGCCTTTAGCTATGACCATCGTATTATTGACGGTAAAGAGGCAGTTGG
TTTTCTGATCAAAATCAAAGATGCTATAGAACAACCAGAACAGCTTCTTGATTTTTAA

Upstream 100 bases:

>100_bases
TTAGCAGACCTAGAAGTAGTTCAACAGCTACGGGTTCCGCACGTCTTAGCCAACAAGAATTTTTAACATTAATGGAAACA
TTGTTTTCTTTAGGTAATGT

Downstream 100 bases:

>100_bases
AATCACAAGATCATCTAGGAAGAAGCAGAACAGCAGTGACGTTGTTCTTTTTTATAGGTGTATCTATCACGTTAGGTTCT
GAATAGTAGTTGCGGTACCG

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MITEVRIPNVAESISEVTIASLLVTSESLVQENQGIMEIESEKVNQLIYAPISGRIVWSVAEGDVVAVGGIVARIYDANE
SVSESTVKDTPVGETVDAEIICFPRSTAHNPPSEGKTFVPLREKMQEEPQRSGAKNEVRERMSSIRKTISRRLVTALHES
AMLTTFNEIHMTPLMKLRKEKQEAFSARYNVKLGLMSFFIKAVIEALKAYPRVNAYIDGDEIVYRQYYDISIAVGTERGL
VVPVIREADKLSSGDIEMKLADLASRARDGLISLPELEGGSFTITNGGVYGSLLSTPIINPPQVGILGMHKIEKRPVVID
NTIAIADMMYVAFSYDHRIIDGKEAVGFLIKIKDAIEQPEQLLDF

Sequences:

>Translated_365_residues
MITEVRIPNVAESISEVTIASLLVTSESLVQENQGIMEIESEKVNQLIYAPISGRIVWSVAEGDVVAVGGIVARIYDANE
SVSESTVKDTPVGETVDAEIICFPRSTAHNPPSEGKTFVPLREKMQEEPQRSGAKNEVRERMSSIRKTISRRLVTALHES
AMLTTFNEIHMTPLMKLRKEKQEAFSARYNVKLGLMSFFIKAVIEALKAYPRVNAYIDGDEIVYRQYYDISIAVGTERGL
VVPVIREADKLSSGDIEMKLADLASRARDGLISLPELEGGSFTITNGGVYGSLLSTPIINPPQVGILGMHKIEKRPVVID
NTIAIADMMYVAFSYDHRIIDGKEAVGFLIKIKDAIEQPEQLLDF
>Mature_365_residues
MITEVRIPNVAESISEVTIASLLVTSESLVQENQGIMEIESEKVNQLIYAPISGRIVWSVAEGDVVAVGGIVARIYDANE
SVSESTVKDTPVGETVDAEIICFPRSTAHNPPSEGKTFVPLREKMQEEPQRSGAKNEVRERMSSIRKTISRRLVTALHES
AMLTTFNEIHMTPLMKLRKEKQEAFSARYNVKLGLMSFFIKAVIEALKAYPRVNAYIDGDEIVYRQYYDISIAVGTERGL
VVPVIREADKLSSGDIEMKLADLASRARDGLISLPELEGGSFTITNGGVYGSLLSTPIINPPQVGILGMHKIEKRPVVID
NTIAIADMMYVAFSYDHRIIDGKEAVGFLIKIKDAIEQPEQLLDF

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=233, Percent_Identity=52.3605150214592, Blast_Score=248, Evalue=6e-66,
Organism=Homo sapiens, GI31711992, Length=227, Percent_Identity=32.15859030837, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI110671329, Length=200, Percent_Identity=35, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI203098753, Length=227, Percent_Identity=30.3964757709251, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI203098816, Length=227, Percent_Identity=30.3964757709251, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI260898739, Length=159, Percent_Identity=33.9622641509434, Blast_Score=86, Evalue=4e-17,
Organism=Escherichia coli, GI1786946, Length=229, Percent_Identity=55.8951965065502, Blast_Score=287, Evalue=7e-79,
Organism=Escherichia coli, GI1786305, Length=219, Percent_Identity=32.8767123287671, Blast_Score=125, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI25146366, Length=228, Percent_Identity=50.8771929824561, Blast_Score=243, Evalue=8e-65,
Organism=Caenorhabditis elegans, GI17537937, Length=266, Percent_Identity=30.4511278195489, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17560088, Length=229, Percent_Identity=32.3144104803493, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17538894, Length=223, Percent_Identity=30.9417040358744, Blast_Score=104, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6320352, Length=400, Percent_Identity=34.75, Blast_Score=248, Evalue=1e-66,
Organism=Saccharomyces cerevisiae, GI6324258, Length=228, Percent_Identity=29.3859649122807, Blast_Score=110, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24645909, Length=227, Percent_Identity=53.7444933920705, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI18859875, Length=225, Percent_Identity=32.8888888888889, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI20129315, Length=228, Percent_Identity=28.0701754385965, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24582497, Length=228, Percent_Identity=28.0701754385965, Blast_Score=102, Evalue=6e-22,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 40359; Mature: 40359

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITEVRIPNVAESISEVTIASLLVTSESLVQENQGIMEIESEKVNQLIYAPISGRIVWSV
CCCEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHCEEEEECCCCEEEEEE
AEGDVVAVGGIVARIYDANESVSESTVKDTPVGETVDAEIICFPRSTAHNPPSEGKTFVP
CCCCEEEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEECH
LREKMQEEPQRSGAKNEVRERMSSIRKTISRRLVTALHESAMLTTFNEIHMTPLMKLRKE
HHHHHHHCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQEAFSARYNVKLGLMSFFIKAVIEALKAYPRVNAYIDGDEIVYRQYYDISIAVGTERGL
HHHHHHHHCEEEHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEEEEEEEEEEECCCCE
VVPVIREADKLSSGDIEMKLADLASRARDGLISLPELEGGSFTITNGGVYGSLLSTPIIN
EEEEECCCCCCCCCCEEEHHHHHHHHHHCCCEECCCCCCCEEEEECCCEEHHHHHCCCCC
PPQVGILGMHKIEKRPVVIDNTIAIADMMYVAFSYDHRIIDGKEAVGFLIKIKDAIEQPE
CCCCCEEEHHHHCCCCEEEECHHHHHHHHHHHHCCCCEEECCHHHHEEEEEEHHHHCCHH
QLLDF
HHHCC
>Mature Secondary Structure
MITEVRIPNVAESISEVTIASLLVTSESLVQENQGIMEIESEKVNQLIYAPISGRIVWSV
CCCEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHCEEEEECCCCEEEEEE
AEGDVVAVGGIVARIYDANESVSESTVKDTPVGETVDAEIICFPRSTAHNPPSEGKTFVP
CCCCEEEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEECH
LREKMQEEPQRSGAKNEVRERMSSIRKTISRRLVTALHESAMLTTFNEIHMTPLMKLRKE
HHHHHHHCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQEAFSARYNVKLGLMSFFIKAVIEALKAYPRVNAYIDGDEIVYRQYYDISIAVGTERGL
HHHHHHHHCEEEHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEEEEEEEEEEECCCCE
VVPVIREADKLSSGDIEMKLADLASRARDGLISLPELEGGSFTITNGGVYGSLLSTPIIN
EEEEECCCCCCCCCCEEEHHHHHHHHHHCCCEECCCCCCCEEEEECCCEEHHHHHCCCCC
PPQVGILGMHKIEKRPVVIDNTIAIADMMYVAFSYDHRIIDGKEAVGFLIKIKDAIEQPE
CCCCCEEEHHHHCCCCEEEECHHHHHHHHHHHHCCCCEEECCHHHHEEEEEEHHHHCCHH
QLLDF
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]