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The map label for this gene is mraW

Identifier: 62184992

GI number: 62184992

Start: 415500

End: 416396

Strand: Direct

Name: mraW

Synonym: CAB361

Alternate gene names: 62184992

Gene position: 415500-416396 (Clockwise)

Preceding gene: 62184983

Following gene: 62184993

Centisome position: 36.31

GC content: 41.36

Gene sequence:

>897_bases
GTGTCTGCAACCCCTTCTCATATTCCGGTATTGGTAAATGAATGTTTGTCTTTATTTGCCGATCGTCATCCAAAATTTTT
CTGTGATGTTACTGTAGGAGCCGGAGGTCACGCTGAAGCTTTTCTCTCCGCATATCCTTCTATAGTTTCTTATGATGCAT
CTGATCGCGACGTGGCAGCTTTATCTATGGCTAAGGAGCATTTAGAAAAATTTGGCGATCGTGTGCATTTTCATCATGCA
TCATTTGAAGATCTTTCTAAAGATCCTAGGGAACATGTGTATGACGGCATACTTGCCGATCTTGGCGTTTCCTCTATGCA
GCTAGATAATCTATCTCGGGGATTTAGTTTTCAAGGAGACGATCATGCTTTAGATATGCGCATGGATGTGACTAAGGGAA
TCACGGCGAGCGAGGTTTTACATACACTTCGTGAAGAAGAGTTAGGGAAAATTTTTCGTGAATACGGAGAGGAACCTCAG
TGGAAAAACGCGGCTAAGGCGATAGTACATTTTAGGCGTCGTAAGAAGATCGTGACGGTTAGAGACTTAAAAGAAGCTAC
AGCCAAAGTTTTTCCTTCTTATCGTCTGCGCAAGAAAATTCATCCATTAACTTTGATTTTTCAAGCTTTACGGGTGTATG
TAAATCAAGAGGACGTGCAATTGAAAGTATTGCTGGAGTCGGCTATGCGTTGGCTTGCTCCTGGAGGGCGGTTGATCATT
ATTTCGTTTTGTAGTTCCGAAGATCGCCCAGTAAAATGGTTTTTTAGAGAGGCTGAGAAATCCGGATTAGGAATGATTCT
GACTAAGAAAGTGATCATGCCTACATATGAAGAAATTAGGAAAAATCCTCGATGTAGATCGGCAAAGCTCCGTTGTTTTG
AAAAGAAATTTTTATGA

Upstream 100 bases:

>100_bases
TAAAATAGAGAATGAGGAATCAATAGAAATCTTTTCGCATTCTCTCTAATGGTGTATCTTTGTCTTGCATTGATGTTTTT
AGAATGATTTAGGCGTTTTT

Downstream 100 bases:

>100_bases
GTAATTATCGTTTTGTACGTCTATTCTTATGTTTTTGTTTTCTAGGCGGATTGCTTTACTCCTATATTAACAAACAAAAC
GATCTGACTAAATTACGCTT

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MSATPSHIPVLVNECLSLFADRHPKFFCDVTVGAGGHAEAFLSAYPSIVSYDASDRDVAALSMAKEHLEKFGDRVHFHHA
SFEDLSKDPREHVYDGILADLGVSSMQLDNLSRGFSFQGDDHALDMRMDVTKGITASEVLHTLREEELGKIFREYGEEPQ
WKNAAKAIVHFRRRKKIVTVRDLKEATAKVFPSYRLRKKIHPLTLIFQALRVYVNQEDVQLKVLLESAMRWLAPGGRLII
ISFCSSEDRPVKWFFREAEKSGLGMILTKKVIMPTYEEIRKNPRCRSAKLRCFEKKFL

Sequences:

>Translated_298_residues
MSATPSHIPVLVNECLSLFADRHPKFFCDVTVGAGGHAEAFLSAYPSIVSYDASDRDVAALSMAKEHLEKFGDRVHFHHA
SFEDLSKDPREHVYDGILADLGVSSMQLDNLSRGFSFQGDDHALDMRMDVTKGITASEVLHTLREEELGKIFREYGEEPQ
WKNAAKAIVHFRRRKKIVTVRDLKEATAKVFPSYRLRKKIHPLTLIFQALRVYVNQEDVQLKVLLESAMRWLAPGGRLII
ISFCSSEDRPVKWFFREAEKSGLGMILTKKVIMPTYEEIRKNPRCRSAKLRCFEKKFL
>Mature_297_residues
SATPSHIPVLVNECLSLFADRHPKFFCDVTVGAGGHAEAFLSAYPSIVSYDASDRDVAALSMAKEHLEKFGDRVHFHHAS
FEDLSKDPREHVYDGILADLGVSSMQLDNLSRGFSFQGDDHALDMRMDVTKGITASEVLHTLREEELGKIFREYGEEPQW
KNAAKAIVHFRRRKKIVTVRDLKEATAKVFPSYRLRKKIHPLTLIFQALRVYVNQEDVQLKVLLESAMRWLAPGGRLIII
SFCSSEDRPVKWFFREAEKSGLGMILTKKVIMPTYEEIRKNPRCRSAKLRCFEKKFL

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=334, Percent_Identity=35.0299401197605, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI165377202, Length=196, Percent_Identity=36.2244897959184, Blast_Score=109, Evalue=3e-24,
Organism=Escherichia coli, GI1786270, Length=311, Percent_Identity=38.9067524115756, Blast_Score=169, Evalue=2e-43,
Organism=Drosophila melanogaster, GI62472493, Length=331, Percent_Identity=34.1389728096677, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI28571637, Length=331, Percent_Identity=34.1389728096677, Blast_Score=135, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_CHLAB (Q5L6B5)

Other databases:

- EMBL:   CR848038
- RefSeq:   YP_219777.1
- ProteinModelPortal:   Q5L6B5
- GeneID:   3338156
- GenomeReviews:   CR848038_GR
- KEGG:   cab:CAB361
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- BioCyc:   CABO218497:CAB361-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 34086; Mature: 33955

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: NA

Important sites: BINDING 55-55 BINDING 82-82 BINDING 100-100 BINDING 107-107

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATPSHIPVLVNECLSLFADRHPKFFCDVTVGAGGHAEAFLSAYPSIVSYDASDRDVAA
CCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHCCHHHCCCCCCCHHHH
LSMAKEHLEKFGDRVHFHHASFEDLSKDPREHVYDGILADLGVSSMQLDNLSRGFSFQGD
HHHHHHHHHHHHHHEEECCCCHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCC
DHALDMRMDVTKGITASEVLHTLREEELGKIFREYGEEPQWKNAAKAIVHFRRRKKIVTV
CCEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEHH
RDLKEATAKVFPSYRLRKKIHPLTLIFQALRVYVNQEDVQLKVLLESAMRWLAPGGRLII
HHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEE
ISFCSSEDRPVKWFFREAEKSGLGMILTKKVIMPTYEEIRKNPRCRSAKLRCFEKKFL
EEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SATPSHIPVLVNECLSLFADRHPKFFCDVTVGAGGHAEAFLSAYPSIVSYDASDRDVAA
CCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHCCHHHCCCCCCCHHHH
LSMAKEHLEKFGDRVHFHHASFEDLSKDPREHVYDGILADLGVSSMQLDNLSRGFSFQGD
HHHHHHHHHHHHHHEEECCCCHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCC
DHALDMRMDVTKGITASEVLHTLREEELGKIFREYGEEPQWKNAAKAIVHFRRRKKIVTV
CCEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEHH
RDLKEATAKVFPSYRLRKKIHPLTLIFQALRVYVNQEDVQLKVLLESAMRWLAPGGRLII
HHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEE
ISFCSSEDRPVKWFFREAEKSGLGMILTKKVIMPTYEEIRKNPRCRSAKLRCFEKKFL
EEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA