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The map label for this gene is gcvH [H]

Identifier: 62184981

GI number: 62184981

Start: 406546

End: 406887

Strand: Direct

Name: gcvH [H]

Synonym: CAB350

Alternate gene names: 62184981

Gene position: 406546-406887 (Clockwise)

Preceding gene: 62184980

Following gene: 62184982

Centisome position: 35.53

GC content: 39.18

Gene sequence:

>342_bases
ATGTGGTATTCTGATTATCATGTGTGGATCGACCCCATACACGAGAATATTGTACGTTTAGGATTGACGTCTAGGATGCG
AGAAAATCTGGGGCAAATCCTTCATATTGACCTGCCGGCTTTAGGAAGCTTCTGTAAGGAAGGTGAGGTGCTTGTCATTT
TGGAATCTTCAAAATCTGCTATTGAGGTATTAAGTCCGGTTTCTGGAGAAATTCTTGAGGTTAATGAGAATCTCAAAGAG
GACGTACGGCTTCTGAATAATTCTCCTGAAGAAGCTGGCTGGTTTGTTATCGTTAAATTAGATCAAAAATTAAATATACA
AAACCTTTCTCCCAAAGAATAG

Upstream 100 bases:

>100_bases
AGCCCACGAGCGTGGAAAAGTTGTTCCTTACTAAAAACCATTTAAACGAAGAAATCCTTGGTAGTTATATAGAACATTTC
ATTGATGAAGGAGTCAGATA

Downstream 100 bases:

>100_bases
TTGGGTTATCTTTTCAAAAAAATATTGTATAGTTTATATTTTTTGAACTTGGATTAGAGTTTTTTTTTATCTTTTGAAGG
AGTAAAATCTTTCCCTGAAA

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 113; Mature: 113

Protein sequence:

>113_residues
MWYSDYHVWIDPIHENIVRLGLTSRMRENLGQILHIDLPALGSFCKEGEVLVILESSKSAIEVLSPVSGEILEVNENLKE
DVRLLNNSPEEAGWFVIVKLDQKLNIQNLSPKE

Sequences:

>Translated_113_residues
MWYSDYHVWIDPIHENIVRLGLTSRMRENLGQILHIDLPALGSFCKEGEVLVILESSKSAIEVLSPVSGEILEVNENLKE
DVRLLNNSPEEAGWFVIVKLDQKLNIQNLSPKE
>Mature_113_residues
MWYSDYHVWIDPIHENIVRLGLTSRMRENLGQILHIDLPALGSFCKEGEVLVILESSKSAIEVLSPVSGEILEVNENLKE
DVRLLNNSPEEAGWFVIVKLDQKLNIQNLSPKE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=111, Percent_Identity=31.5315315315315, Blast_Score=64, Evalue=2e-11,
Organism=Homo sapiens, GI89057342, Length=111, Percent_Identity=31.5315315315315, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI1789271, Length=107, Percent_Identity=31.7757009345794, Blast_Score=74, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17551294, Length=92, Percent_Identity=35.8695652173913, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17507493, Length=107, Percent_Identity=29.9065420560748, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319272, Length=107, Percent_Identity=32.7102803738318, Blast_Score=74, Evalue=4e-15,
Organism=Drosophila melanogaster, GI17865652, Length=107, Percent_Identity=32.7102803738318, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002930
- InterPro:   IPR017514
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 12866; Mature: 12866

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWYSDYHVWIDPIHENIVRLGLTSRMRENLGQILHIDLPALGSFCKEGEVLVILESSKSA
CCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHCCCCCEEEEECCCCCH
IEVLSPVSGEILEVNENLKEDVRLLNNSPEEAGWFVIVKLDQKLNIQNLSPKE
HHHHCCCCCCEEECCCCHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MWYSDYHVWIDPIHENIVRLGLTSRMRENLGQILHIDLPALGSFCKEGEVLVILESSKSA
CCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHCCCCCEEEEECCCCCH
IEVLSPVSGEILEVNENLKEDVRLLNNSPEEAGWFVIVKLDQKLNIQNLSPKE
HHHHCCCCCCEEECCCCHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]