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The map label for this gene is def

Identifier: 62184939

GI number: 62184939

Start: 356666

End: 357220

Strand: Direct

Name: def

Synonym: CAB307

Alternate gene names: 62184939

Gene position: 356666-357220 (Clockwise)

Preceding gene: 62184938

Following gene: 62184948

Centisome position: 31.17

GC content: 40.18

Gene sequence:

>555_bases
ATGATTAGAGAATTAGAATATTACGGTAGCCCTACGTTACGTAGAAAGGCGGAGGCTATTCTTGAAATTACTGATGAGAT
TCGTCAGTTGGCTCAAGATATGTATGAGACTATGGTCGCTCATAAGGGAGTGGGTTTAGCAGCTCCTCAGGTAGGGGAAA
GCGTAAGTCTTTTTGTTATGTGTGTTGAGGGAGAAACAGAGGATGGGGATCTGATTTTTTGTGACTTCCCCAAGGTATAT
ATTAATCCTGTACTTTCTAATGTTTCCGAGGATCTTGTTTTAGGTAGGGAAGGATGTTTATCAATTCCCGGGTTGCGCGC
TGATGTCTATCGTCCGCGTTCCATTACCGTAAAAGCGATCAATCTCGATGGTCAAGAATTCACGGAACATTTAGAAGGGT
TTCCTGCGCGTATTGTCATGCATGAAAATGACCATCTCAATGGAATTTTATACATTGATAAGATGGAAGAACCTAAGGAT
TATAAGAAGTTTAAGTCTGCCTTAGAAAAAATTCGCCGTCGTTATAATAATCATATCACAGATAAAGCTTCTTAG

Upstream 100 bases:

>100_bases
TCAAAAAGAGATGGAGAACCCTTCGCCGTTGCCTACAGTCTGTCCCTTTTATTTAATAGAAGATGCGAATGAGTAGCCAA
TCTGCGGGGTGATATAAATC

Downstream 100 bases:

>100_bases
TGGTATCGTCTCAAGCTTGCTTGTCTTATTGATTTCTCCAAGTGCAAAAGAAGCCGTAAACCCTTTGAGCACAGGATTAC
TGGTGGCTTAGGTGTTTGGG

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MIRELEYYGSPTLRRKAEAILEITDEIRQLAQDMYETMVAHKGVGLAAPQVGESVSLFVMCVEGETEDGDLIFCDFPKVY
INPVLSNVSEDLVLGREGCLSIPGLRADVYRPRSITVKAINLDGQEFTEHLEGFPARIVMHENDHLNGILYIDKMEEPKD
YKKFKSALEKIRRRYNNHITDKAS

Sequences:

>Translated_184_residues
MIRELEYYGSPTLRRKAEAILEITDEIRQLAQDMYETMVAHKGVGLAAPQVGESVSLFVMCVEGETEDGDLIFCDFPKVY
INPVLSNVSEDLVLGREGCLSIPGLRADVYRPRSITVKAINLDGQEFTEHLEGFPARIVMHENDHLNGILYIDKMEEPKD
YKKFKSALEKIRRRYNNHITDKAS
>Mature_184_residues
MIRELEYYGSPTLRRKAEAILEITDEIRQLAQDMYETMVAHKGVGLAAPQVGESVSLFVMCVEGETEDGDLIFCDFPKVY
INPVLSNVSEDLVLGREGCLSIPGLRADVYRPRSITVKAINLDGQEFTEHLEGFPARIVMHENDHLNGILYIDKMEEPKD
YKKFKSALEKIRRRYNNHITDKAS

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=160, Percent_Identity=31.875, Blast_Score=80, Evalue=9e-16,
Organism=Escherichia coli, GI1789682, Length=161, Percent_Identity=36.0248447204969, Blast_Score=87, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24645728, Length=165, Percent_Identity=35.7575757575758, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24645726, Length=159, Percent_Identity=30.8176100628931, Blast_Score=76, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_CHLAB (Q5L6G8)

Other databases:

- EMBL:   CR848038
- RefSeq:   YP_219724.1
- HSSP:   P43522
- ProteinModelPortal:   Q5L6G8
- SMR:   Q5L6G8
- GeneID:   3337642
- GenomeReviews:   CR848038_GR
- KEGG:   cab:CAB307
- HOGENOM:   HBG665227
- OMA:   VINPTWE
- ProtClustDB:   PRK00150
- BioCyc:   CABO218497:CAB307-MONOMER
- BRENDA:   3.5.1.88
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 20950; Mature: 20950

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: ACT_SITE 142-142

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRELEYYGSPTLRRKAEAILEITDEIRQLAQDMYETMVAHKGVGLAAPQVGESVSLFVM
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEE
CVEGETEDGDLIFCDFPKVYINPVLSNVSEDLVLGREGCLSIPGLRADVYRPRSITVKAI
EEECCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEE
NLDGQEFTEHLEGFPARIVMHENDHLNGILYIDKMEEPKDYKKFKSALEKIRRRYNNHIT
ECCHHHHHHHHCCCCEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCC
DKAS
CCCC
>Mature Secondary Structure
MIRELEYYGSPTLRRKAEAILEITDEIRQLAQDMYETMVAHKGVGLAAPQVGESVSLFVM
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEE
CVEGETEDGDLIFCDFPKVYINPVLSNVSEDLVLGREGCLSIPGLRADVYRPRSITVKAI
EEECCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEE
NLDGQEFTEHLEGFPARIVMHENDHLNGILYIDKMEEPKDYKKFKSALEKIRRRYNNHIT
ECCHHHHHHHHCCCCEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCC
DKAS
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA