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The map label for this gene is dnaF [H]

Identifier: 62184729

GI number: 62184729

Start: 100784

End: 101533

Strand: Direct

Name: dnaF [H]

Synonym: CAB085

Alternate gene names: 62184729

Gene position: 100784-101533 (Clockwise)

Preceding gene: 62184728

Following gene: 62184730

Centisome position: 8.81

GC content: 38.8

Gene sequence:

>750_bases
ATGACTTTACTAAAAGATACAGTTTTTGTTTGCCTAGATTGTGAGATGACTGGGTTGGATGTAAAGAAAGATCGTATTAT
TGAATTTGCTGCGATACGTTTCACTTTTGATAGCGTGATTGATTCTATGGAGACATTAATCAATCCCGACCGTGTCATTT
CCGCCGAATCACAGAGAATACATCATATTTCCGACGCGATGTTAAAAGATCAGCCTAGGATTGCGGAAGTATTTCCAAAG
ATTAAATCTTTTTTGAAAGAAGGAGACTATATCGTAGGCCATAGCGTAGGATTCGATCTCCAGGTATTGAGTCAAGAAGC
AGAAAGGATAGGGGAGACCTTCTTATCTAAGTACTACATCATCGACACCTTACGATTGGCGAAAGAATACGGAGATAGCC
CGAATAATTCTTTGGAAGCCCTAGCTGTACACTTCAATGTTCCCTATGACGGTAACCATCGCGCTATGAAAGATGTTGAG
ATCAATATCAGCATTTTCAAACATTTCTGTAAGCGCTTCCGGACGATAGAACAACTAAAGCAAATGCTATCAAAACCGAT
AAAGATGAAATATATGCCTTTAGGAAAACATAAAGGACGTTGTTTCTCCGAAATCCCCCTCTCCTATTTGCAGTGGGCAT
CTAAAATGGATTTCGATCCCGATTTACTCTTTTCTATAAGACATGAGATCAAACATAGACAAAAAGGCATAGGTTTTACG
CAAGTCAATAACCCATTTATTGGCTTGTAA

Upstream 100 bases:

>100_bases
ACAGGTGCAGATTCAACCGCTAACCACTGTGCAAAGAGAAGTTAGCATAGATGCCCCACCCTCCATATTGTTAAAATTGC
TGAAGGAATAAAATAGGATC

Downstream 100 bases:

>100_bases
AACACTTTTATTTTATAGAAGTTGATTTGAAATCTTTGAACTTCCCTCTAGTAAAAAAATACAAATCACTCTTTAAGTAA
TCTCTTGATTTTCTTTAGAC

Product: DNA polymerase III subunit epsilon

Products: NA

Alternate protein names: PolIII [H]

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MTLLKDTVFVCLDCEMTGLDVKKDRIIEFAAIRFTFDSVIDSMETLINPDRVISAESQRIHHISDAMLKDQPRIAEVFPK
IKSFLKEGDYIVGHSVGFDLQVLSQEAERIGETFLSKYYIIDTLRLAKEYGDSPNNSLEALAVHFNVPYDGNHRAMKDVE
INISIFKHFCKRFRTIEQLKQMLSKPIKMKYMPLGKHKGRCFSEIPLSYLQWASKMDFDPDLLFSIRHEIKHRQKGIGFT
QVNNPFIGL

Sequences:

>Translated_249_residues
MTLLKDTVFVCLDCEMTGLDVKKDRIIEFAAIRFTFDSVIDSMETLINPDRVISAESQRIHHISDAMLKDQPRIAEVFPK
IKSFLKEGDYIVGHSVGFDLQVLSQEAERIGETFLSKYYIIDTLRLAKEYGDSPNNSLEALAVHFNVPYDGNHRAMKDVE
INISIFKHFCKRFRTIEQLKQMLSKPIKMKYMPLGKHKGRCFSEIPLSYLQWASKMDFDPDLLFSIRHEIKHRQKGIGFT
QVNNPFIGL
>Mature_248_residues
TLLKDTVFVCLDCEMTGLDVKKDRIIEFAAIRFTFDSVIDSMETLINPDRVISAESQRIHHISDAMLKDQPRIAEVFPKI
KSFLKEGDYIVGHSVGFDLQVLSQEAERIGETFLSKYYIIDTLRLAKEYGDSPNNSLEALAVHFNVPYDGNHRAMKDVEI
NISIFKHFCKRFRTIEQLKQMLSKPIKMKYMPLGKHKGRCFSEIPLSYLQWASKMDFDPDLLFSIRHEIKHRQKGIGFTQ
VNNPFIGL

Specific function: Required for replicative DNA synthesis. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1788149, Length=232, Percent_Identity=27.5862068965517, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011708
- InterPro:   IPR006054
- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR023223
- InterPro:   IPR016027
- InterPro:   IPR004013
- InterPro:   IPR003141
- InterPro:   IPR006308
- InterPro:   IPR012337 [H]

Pfam domain/function: PF07733 DNA_pol3_alpha; PF00929 Exonuc_X-T; PF02811 PHP [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 28795; Mature: 28664

Theoretical pI: Translated: 8.01; Mature: 8.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLLKDTVFVCLDCEMTGLDVKKDRIIEFAAIRFTFDSVIDSMETLINPDRVISAESQRI
CCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHH
HHISDAMLKDQPRIAEVFPKIKSFLKEGDYIVGHSVGFDLQVLSQEAERIGETFLSKYYI
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
IDTLRLAKEYGDSPNNSLEALAVHFNVPYDGNHRAMKDVEINISIFKHFCKRFRTIEQLK
HHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCEEHEEEEHHHHHHHHHHHHHHHHHHH
QMLSKPIKMKYMPLGKHKGRCFSEIPLSYLQWASKMDFDPDLLFSIRHEIKHRQKGIGFT
HHHCCCCEEEEEECCCCCCCCHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEE
QVNNPFIGL
ECCCCCCCC
>Mature Secondary Structure 
TLLKDTVFVCLDCEMTGLDVKKDRIIEFAAIRFTFDSVIDSMETLINPDRVISAESQRI
CCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHH
HHISDAMLKDQPRIAEVFPKIKSFLKEGDYIVGHSVGFDLQVLSQEAERIGETFLSKYYI
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
IDTLRLAKEYGDSPNNSLEALAVHFNVPYDGNHRAMKDVEINISIFKHFCKRFRTIEQLK
HHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCEEHEEEEHHHHHHHHHHHHHHHHHHH
QMLSKPIKMKYMPLGKHKGRCFSEIPLSYLQWASKMDFDPDLLFSIRHEIKHRQKGIGFT
HHHCCCCEEEEEECCCCCCCCHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEE
QVNNPFIGL
ECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]