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The map label for this gene is gcvA [H]

Identifier: 59714249

GI number: 59714249

Start: 1202264

End: 1203163

Strand: Reverse

Name: gcvA [H]

Synonym: VF_A1066

Alternate gene names: 59714249

Gene position: 1203163-1202264 (Counterclockwise)

Preceding gene: 59714251

Following gene: 59714248

Centisome position: 90.44

GC content: 37.44

Gene sequence:

>900_bases
ATGAAGTTGCACCATTTAAATGGTTTGCGAGCCTTTGAAGCTTCAGCACGACATTTGAGTTTTTCTTTAGCGGCGAAAGA
GTTAAACGTTACTCCCGCAGCGATCGGGCAGCAAGTAAAACTGTTGGAAGAATGGTTAGGTATTTCTTTATTCGAAAGAC
GAACATCAGGAGTTTCTCGTTTAGTATTAACTAAAGAAGCACTGTTAGGTCTACCTGAAATTACGCGTGGATTTGAGCAT
TTAGCACAAGGTTTAGATTTATTAAAACCAATGAAAAACAGTCCTATTTTAACGGTAGCGGTAAGTCCTGCATTTGCGGC
TAAATGGTTATTAATGCGCATAGATGATTTTCAAACTTCTTATTCAGAGTGGGATTTAAGGCTAGATACAAATATTCGAA
CTGTGGATTACCTTACAGAAGGAATCGATGTTGGGATTCGTTATGGAAAAGGTCAGTGGGAAGGATTAGAAGCATCGCTA
TTAATGACAGAGTCTGTTTTTCCAGTATGTTCTCCTGAGTTACTTAATAAAGGCATTGAAGGTATTGAGTCTATTGCTCA
ATTTCCTTTATTGCATGATATCTCTCTGCCTAAAGAAAGCGGCTTTCCTTCTTGGAAGGTGTGGTTTGAGCAGAATAGTA
TTTTTGATGTAAATACAGAAAAAGGACTAAAAATAAATAACTCAGCTTCTGTTATTCAAGCTGCAATTAATGGACAAGGC
ATAGCTTTGGGGCGAAGTGTATTAGTAAAAGATGATTTAGATTGCGGACGATTAGTACAGTTGTTTCCTGATCTTGAATC
GACCACAGAGTTAGCCTATTACGTTGTGTGGAAACAGAAAGGAATTGTGAGTGATAAAATCAGCGTATTTAAAGAATGGT
TACATCAGCAAGTAGAATAA

Upstream 100 bases:

>100_bases
AAGTTTATTGTGTGATTTTTATCTCGTACTCACAAATCAGAACTTCTATACTTCCAGTAAAGTTTTTCTTTATTGATGTT
TATTTTTAGGGAAGTGTTTT

Downstream 100 bases:

>100_bases
ATTGATAACTTAATGGTATGTGGTTGCCCTGTCCCATCTTCATTTTGAATGCATCTGTTTAATTTTTATTATTAATGCTT
GTTTTATAGTCATTTAGTTG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MKLHHLNGLRAFEASARHLSFSLAAKELNVTPAAIGQQVKLLEEWLGISLFERRTSGVSRLVLTKEALLGLPEITRGFEH
LAQGLDLLKPMKNSPILTVAVSPAFAAKWLLMRIDDFQTSYSEWDLRLDTNIRTVDYLTEGIDVGIRYGKGQWEGLEASL
LMTESVFPVCSPELLNKGIEGIESIAQFPLLHDISLPKESGFPSWKVWFEQNSIFDVNTEKGLKINNSASVIQAAINGQG
IALGRSVLVKDDLDCGRLVQLFPDLESTTELAYYVVWKQKGIVSDKISVFKEWLHQQVE

Sequences:

>Translated_299_residues
MKLHHLNGLRAFEASARHLSFSLAAKELNVTPAAIGQQVKLLEEWLGISLFERRTSGVSRLVLTKEALLGLPEITRGFEH
LAQGLDLLKPMKNSPILTVAVSPAFAAKWLLMRIDDFQTSYSEWDLRLDTNIRTVDYLTEGIDVGIRYGKGQWEGLEASL
LMTESVFPVCSPELLNKGIEGIESIAQFPLLHDISLPKESGFPSWKVWFEQNSIFDVNTEKGLKINNSASVIQAAINGQG
IALGRSVLVKDDLDCGRLVQLFPDLESTTELAYYVVWKQKGIVSDKISVFKEWLHQQVE
>Mature_299_residues
MKLHHLNGLRAFEASARHLSFSLAAKELNVTPAAIGQQVKLLEEWLGISLFERRTSGVSRLVLTKEALLGLPEITRGFEH
LAQGLDLLKPMKNSPILTVAVSPAFAAKWLLMRIDDFQTSYSEWDLRLDTNIRTVDYLTEGIDVGIRYGKGQWEGLEASL
LMTESVFPVCSPELLNKGIEGIESIAQFPLLHDISLPKESGFPSWKVWFEQNSIFDVNTEKGLKINNSASVIQAAINGQG
IALGRSVLVKDDLDCGRLVQLFPDLESTTELAYYVVWKQKGIVSDKISVFKEWLHQQVE

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=295, Percent_Identity=37.6271186440678, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI1786448, Length=293, Percent_Identity=34.8122866894198, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1788706, Length=297, Percent_Identity=31.6498316498317, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI87081978, Length=297, Percent_Identity=27.6094276094276, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1786401, Length=284, Percent_Identity=24.2957746478873, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI145693193, Length=304, Percent_Identity=24.0131578947368, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789440, Length=279, Percent_Identity=25.089605734767, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33437; Mature: 33437

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLHHLNGLRAFEASARHLSFSLAAKELNVTPAAIGQQVKLLEEWLGISLFERRTSGVSR
CCCCCCCCCHHHHHHHHHEEEEEEHHHCCCCHHHHHHHHHHHHHHHCHHHHHHHHCCHHH
LVLTKEALLGLPEITRGFEHLAQGLDLLKPMKNSPILTVAVSPAFAAKWLLMRIDDFQTS
HHEEHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHCC
YSEWDLRLDTNIRTVDYLTEGIDVGIRYGKGQWEGLEASLLMTESVFPVCSPELLNKGIE
CCCEEEEECCCCCHHHHHHCCHHEEEEECCCCCCCCEEEEEEECCCCCCCCHHHHHHHHH
GIESIAQFPLLHDISLPKESGFPSWKVWFEQNSIFDVNTEKGLKINNSASVIQAAINGQG
HHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCEEEECCCCCEEECCCHHHHHHHHCCCC
IALGRSVLVKDDLDCGRLVQLFPDLESTTELAYYVVWKQKGIVSDKISVFKEWLHQQVE
EEECCEEEEECCCCHHHHHHHHCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLHHLNGLRAFEASARHLSFSLAAKELNVTPAAIGQQVKLLEEWLGISLFERRTSGVSR
CCCCCCCCCHHHHHHHHHEEEEEEHHHCCCCHHHHHHHHHHHHHHHCHHHHHHHHCCHHH
LVLTKEALLGLPEITRGFEHLAQGLDLLKPMKNSPILTVAVSPAFAAKWLLMRIDDFQTS
HHEEHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHCC
YSEWDLRLDTNIRTVDYLTEGIDVGIRYGKGQWEGLEASLLMTESVFPVCSPELLNKGIE
CCCEEEEECCCCCHHHHHHCCHHEEEEECCCCCCCCEEEEEEECCCCCCCCHHHHHHHHH
GIESIAQFPLLHDISLPKESGFPSWKVWFEQNSIFDVNTEKGLKINNSASVIQAAINGQG
HHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCEEEECCCCCEEECCCHHHHHHHHCCCC
IALGRSVLVKDDLDCGRLVQLFPDLESTTELAYYVVWKQKGIVSDKISVFKEWLHQQVE
EEECCEEEEECCCCHHHHHHHHCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]