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The map label for this gene is mifB [C]

Identifier: 59714142

GI number: 59714142

Start: 1078131

End: 1080101

Strand: Reverse

Name: mifB [C]

Synonym: VF_A0959

Alternate gene names: 59714142

Gene position: 1080101-1078131 (Counterclockwise)

Preceding gene: 59714143

Following gene: 59714141

Centisome position: 81.19

GC content: 34.04

Gene sequence:

>1971_bases
ATGATTTCTCGCCCATATGTGAGCTTACGTAAAGCATTCACTTACGTTTTGATTATTTGCTCGCTTGTGCCATTGATATT
TATTAGTCAATTTTTAACTAATAGCATAGGTGAAACGCTATTTAATCAAAAAGAACAGCAAATTCAAAATGCCGTATCTA
CCATTGCGCAAGAGATAGTGGTGGAGTTCGATCGTATTCAGCAAAGCCTCAATTGGCTGTCACGTGACCGATTTAACATT
CAAGCGTTAGATAATATTCTTTATTCTAGTGTAGTTAGGCATAATTTAACTCGGTTTAGTGAACTGTCACGCTTGATTAA
TAGCGTGTACGTTCTCAACGATAAATGGGAACCATTATATAATTTAAACGGTCGTCCATATCATTTTGAAAATAGTCAGT
TACTTAAGCAGATTAATATCGATCATACGATATATGAGAAAGGGCTAACTAAGACACTGTTTTACTCAGAGCCAAACTTA
ATAAAGGAGTCCTCATCTTCTAAAGGAATCGCTTTAGTTACTCCAATGTTAGCTTATAACTTAGAGGGATCTGGTCGTTA
TCGAGCCTCTGGTTATGTTGTCGTGTTGATTTCGTTTGAGACAATAGAAGAGCACTTCCAACATTTTCTATTTAAAGATG
AAAATTTTAAATTAAATCAACATATTGGCAATGAAGATCATTCTGTTAACCAACAAAAAGCAGAAGATGGAAAACTTGAA
CGTATTTTTATATTAAATAATCCTTCATTTTCATCATCGTTGACATTAGATATTACTTACCAATTTTCTGATGTGTCACG
ATTAAAAGACATGAAGTCGTCTTTATTAGCTCTGTTCACTGTTATTGGGTGGACGCTTATTACGGTAATTTGTTTGGCTA
TGGGGATTACCCGTTGGATCTCAAAAGAATTTGGTGAAATGGAAGAGACCATTGTGAGTTACGCAACCAATAAGCCAAAT
AAGCGAGAAAAGCGTTTTCAATTTAGTGAATTTGAGCGAATGGCAATCGTTTTAAAGACAATGAGTTCAACGATTCGTAA
TCAATTACTTGAACTTAAAAAGAAAAATGATGAGCTGAATGAATCTAAAAACTTAATTGAATTAAGTAACGAAAAATTAT
CATCATTTAATCAAGAGCTGGAAATTCAAGTTAAAAAACAGACAGCACAAATTAGTGATGCATTATTTCGTGAAGAGTTA
AATAAAAATAACTTAGCAGCAGTCATTGAGTTTGGCTCTGAATATAAGTCATTAGATTTTCATGATGTACCTGCGAAAGT
TGAGCAACAGCTGAAAAAATTATTCCCAGATATGGTTTGGAAGCTAAGTTTTGACCCTCTAGGAGATAATGCAGGGATAG
AGATTGTTTCAAGTAATTATAAGGTCTTAGCTAGTTTACACTTTGTATCTACAGATGCCTTGGAAAAGCAAGTGCTTGTA
TTTGAACTATTTATTAAGCAATTGTCATCATGGTTAGAATTAGCAGATATTGCCAGAACCGATGTATTACTTGCCTGCGG
AAATAGAAAAGCATTTGATGAGGATTTAGCTCATTACCAAAAGAGATATCGAGATTCTAAAGCGGTTTGGAATTTTGGTT
TGTTCATTTTAGATGTTAATGGATTAAAGTTGATTAATGATCAATATGGACACAGTATTGGTGATGAATTGATTAAAGCG
AGTTGTCATAATATTGCTCAGGTATTATCAAGCGACATTAAGTTTTATCGTATTGGTGGTGATGAGTTTGTTTTGCTTGT
AGAGAATGTAACGCAAGGTGAGTGCCTTCAGTTGTCTGATAAATTGAGAGTGAATCAAAAGGATCTTTCATTCTTCGATA
AAGAAGGGAGCTCTTATGCTGCCCATTATTCTATTGGTTGGGCATGTTCAGATAAAACAGAGTTATTCAAAATGTTTACC
GTTGCTGATGAACATATGTATCGAGAGAAAAAAGCTTATTATCAGCAGTAA

Upstream 100 bases:

>100_bases
CAAGAGGCGTATCTCGTTATTCTGATGGTATGCCTAAAGAAGAAACCATGCTTTATATTAATCAATTGATTGATCGTTTT
TCTAAAAAGGTAGAAGGATC

Downstream 100 bases:

>100_bases
TATTTCATAAACCAACGTGATGGCACTTTATCTTAGATGAAGTGCTATTTTTTTACTTACTAACAAACAGTATTAGCAAA
GTCATCATTTACATTATTAA

Product: diguanylate cyclase (GGDEF), inhibitor of flagellar motility

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 656; Mature: 656

Protein sequence:

>656_residues
MISRPYVSLRKAFTYVLIICSLVPLIFISQFLTNSIGETLFNQKEQQIQNAVSTIAQEIVVEFDRIQQSLNWLSRDRFNI
QALDNILYSSVVRHNLTRFSELSRLINSVYVLNDKWEPLYNLNGRPYHFENSQLLKQINIDHTIYEKGLTKTLFYSEPNL
IKESSSSKGIALVTPMLAYNLEGSGRYRASGYVVVLISFETIEEHFQHFLFKDENFKLNQHIGNEDHSVNQQKAEDGKLE
RIFILNNPSFSSSLTLDITYQFSDVSRLKDMKSSLLALFTVIGWTLITVICLAMGITRWISKEFGEMEETIVSYATNKPN
KREKRFQFSEFERMAIVLKTMSSTIRNQLLELKKKNDELNESKNLIELSNEKLSSFNQELEIQVKKQTAQISDALFREEL
NKNNLAAVIEFGSEYKSLDFHDVPAKVEQQLKKLFPDMVWKLSFDPLGDNAGIEIVSSNYKVLASLHFVSTDALEKQVLV
FELFIKQLSSWLELADIARTDVLLACGNRKAFDEDLAHYQKRYRDSKAVWNFGLFILDVNGLKLINDQYGHSIGDELIKA
SCHNIAQVLSSDIKFYRIGGDEFVLLVENVTQGECLQLSDKLRVNQKDLSFFDKEGSSYAAHYSIGWACSDKTELFKMFT
VADEHMYREKKAYYQQ

Sequences:

>Translated_656_residues
MISRPYVSLRKAFTYVLIICSLVPLIFISQFLTNSIGETLFNQKEQQIQNAVSTIAQEIVVEFDRIQQSLNWLSRDRFNI
QALDNILYSSVVRHNLTRFSELSRLINSVYVLNDKWEPLYNLNGRPYHFENSQLLKQINIDHTIYEKGLTKTLFYSEPNL
IKESSSSKGIALVTPMLAYNLEGSGRYRASGYVVVLISFETIEEHFQHFLFKDENFKLNQHIGNEDHSVNQQKAEDGKLE
RIFILNNPSFSSSLTLDITYQFSDVSRLKDMKSSLLALFTVIGWTLITVICLAMGITRWISKEFGEMEETIVSYATNKPN
KREKRFQFSEFERMAIVLKTMSSTIRNQLLELKKKNDELNESKNLIELSNEKLSSFNQELEIQVKKQTAQISDALFREEL
NKNNLAAVIEFGSEYKSLDFHDVPAKVEQQLKKLFPDMVWKLSFDPLGDNAGIEIVSSNYKVLASLHFVSTDALEKQVLV
FELFIKQLSSWLELADIARTDVLLACGNRKAFDEDLAHYQKRYRDSKAVWNFGLFILDVNGLKLINDQYGHSIGDELIKA
SCHNIAQVLSSDIKFYRIGGDEFVLLVENVTQGECLQLSDKLRVNQKDLSFFDKEGSSYAAHYSIGWACSDKTELFKMFT
VADEHMYREKKAYYQQ
>Mature_656_residues
MISRPYVSLRKAFTYVLIICSLVPLIFISQFLTNSIGETLFNQKEQQIQNAVSTIAQEIVVEFDRIQQSLNWLSRDRFNI
QALDNILYSSVVRHNLTRFSELSRLINSVYVLNDKWEPLYNLNGRPYHFENSQLLKQINIDHTIYEKGLTKTLFYSEPNL
IKESSSSKGIALVTPMLAYNLEGSGRYRASGYVVVLISFETIEEHFQHFLFKDENFKLNQHIGNEDHSVNQQKAEDGKLE
RIFILNNPSFSSSLTLDITYQFSDVSRLKDMKSSLLALFTVIGWTLITVICLAMGITRWISKEFGEMEETIVSYATNKPN
KREKRFQFSEFERMAIVLKTMSSTIRNQLLELKKKNDELNESKNLIELSNEKLSSFNQELEIQVKKQTAQISDALFREEL
NKNNLAAVIEFGSEYKSLDFHDVPAKVEQQLKKLFPDMVWKLSFDPLGDNAGIEIVSSNYKVLASLHFVSTDALEKQVLV
FELFIKQLSSWLELADIARTDVLLACGNRKAFDEDLAHYQKRYRDSKAVWNFGLFILDVNGLKLINDQYGHSIGDELIKA
SCHNIAQVLSSDIKFYRIGGDEFVLLVENVTQGECLQLSDKLRVNQKDLSFFDKEGSSYAAHYSIGWACSDKTELFKMFT
VADEHMYREKKAYYQQ

Specific function: Unknown

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=156, Percent_Identity=26.2820512820513, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR011620 [H]

Pfam domain/function: PF07694 5TM-5TMR_LYT; PF00990 GGDEF [H]

EC number: NA

Molecular weight: Translated: 75684; Mature: 75684

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISRPYVSLRKAFTYVLIICSLVPLIFISQFLTNSIGETLFNQKEQQIQNAVSTIAQEIV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VEFDRIQQSLNWLSRDRFNIQALDNILYSSVVRHNLTRFSELSRLINSVYVLNDKWEPLY
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCC
NLNGRPYHFENSQLLKQINIDHTIYEKGLTKTLFYSEPNLIKESSSSKGIALVTPMLAYN
CCCCCCEECCCHHHHHHHCCCHHHHHCCCCCEEEECCCCHHHCCCCCCCEEEEEHHHHEE
LEGSGRYRASGYVVVLISFETIEEHFQHFLFKDENFKLNQHIGNEDHSVNQQKAEDGKLE
CCCCCCEEECCEEEEEEEHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCHHHCCCCCEE
RIFILNNPSFSSSLTLDITYQFSDVSRLKDMKSSLLALFTVIGWTLITVICLAMGITRWI
EEEEEECCCCCCEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SKEFGEMEETIVSYATNKPNKREKRFQFSEFERMAIVLKTMSSTIRNQLLELKKKNDELN
HHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
ESKNLIELSNEKLSSFNQELEIQVKKQTAQISDALFREELNKNNLAAVIEFGSEYKSLDF
HCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCC
HDVPAKVEQQLKKLFPDMVWKLSFDPLGDNAGIEIVSSNYKVLASLHFVSTDALEKQVLV
CCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCEEEEECCCEEEEEEHHHHHHHHHHHHHH
FELFIKQLSSWLELADIARTDVLLACGNRKAFDEDLAHYQKRYRDSKAVWNFGLFILDVN
HHHHHHHHHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHCCCHHHEEEEEEEEEEC
GLKLINDQYGHSIGDELIKASCHNIAQVLSSDIKFYRIGGDEFVLLVENVTQGECLQLSD
CEEEECCHHCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEECCCCCCHHCCHH
KLRVNQKDLSFFDKEGSSYAAHYSIGWACSDKTELFKMFTVADEHMYREKKAYYQQ
HHCCCHHHHHHHHCCCCCEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MISRPYVSLRKAFTYVLIICSLVPLIFISQFLTNSIGETLFNQKEQQIQNAVSTIAQEIV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VEFDRIQQSLNWLSRDRFNIQALDNILYSSVVRHNLTRFSELSRLINSVYVLNDKWEPLY
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCC
NLNGRPYHFENSQLLKQINIDHTIYEKGLTKTLFYSEPNLIKESSSSKGIALVTPMLAYN
CCCCCCEECCCHHHHHHHCCCHHHHHCCCCCEEEECCCCHHHCCCCCCCEEEEEHHHHEE
LEGSGRYRASGYVVVLISFETIEEHFQHFLFKDENFKLNQHIGNEDHSVNQQKAEDGKLE
CCCCCCEEECCEEEEEEEHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCHHHCCCCCEE
RIFILNNPSFSSSLTLDITYQFSDVSRLKDMKSSLLALFTVIGWTLITVICLAMGITRWI
EEEEEECCCCCCEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SKEFGEMEETIVSYATNKPNKREKRFQFSEFERMAIVLKTMSSTIRNQLLELKKKNDELN
HHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
ESKNLIELSNEKLSSFNQELEIQVKKQTAQISDALFREELNKNNLAAVIEFGSEYKSLDF
HCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCC
HDVPAKVEQQLKKLFPDMVWKLSFDPLGDNAGIEIVSSNYKVLASLHFVSTDALEKQVLV
CCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCEEEEECCCEEEEEEHHHHHHHHHHHHHH
FELFIKQLSSWLELADIARTDVLLACGNRKAFDEDLAHYQKRYRDSKAVWNFGLFILDVN
HHHHHHHHHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHCCCHHHEEEEEEEEEEC
GLKLINDQYGHSIGDELIKASCHNIAQVLSSDIKFYRIGGDEFVLLVENVTQGECLQLSD
CEEEECCHHCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEECCCCCCHHCCHH
KLRVNQKDLSFFDKEGSSYAAHYSIGWACSDKTELFKMFTVADEHMYREKKAYYQQ
HHCCCHHHHHHHHCCCCCEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA