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The map label for this gene is bcsZ [H]

Identifier: 59714065

GI number: 59714065

Start: 996424

End: 997602

Strand: Reverse

Name: bcsZ [H]

Synonym: VF_A0882

Alternate gene names: 59714065

Gene position: 997602-996424 (Counterclockwise)

Preceding gene: 59714066

Following gene: 59714064

Centisome position: 74.99

GC content: 38.25

Gene sequence:

>1179_bases
ATGAAAATGCAATTAACGGTATTGAGTCTATTAATTGGCACGTTAACTTGCTCTTCTTCAGCTATTGCTCAAGATGAAAA
TTACACAACCCCAATCGTTTCATCTGCCGCACTAACAAAAGAGAATCAGTGTGAATGGGGGCAATGGGAAAGCTTTAAAC
AACATTATATAGAGAATGGTCGAGTCGTTGATAACAGTGATCCTCGTTTAATAACGACATCAGAAGGTCAATCTTATGCT
CTATTTTTTGCATTAATTGCGAATGATAAAAAAACATTTGATGAATTACTTGGTTGGACTGAGTTGCATTTAGCTGGGGG
GGATCTAACAGCACAACTCCCTGCTTGGTTATGGGGAAGGCAACCTGATGGCTCGCAAGGTATTTTAGACTCAAACTCAG
CAGCTGACTCAGATTTATGGATTGCTTATAGTCTACTCGAAGCCGGAAGATTGTGGGATAACCATTATTATCAATCTCTT
GGACATTTACTGGCTAGTCGAATTTTACGTGACGAAACCATCAAAGTGTCAGGTCTTGGAACGGTATTATTGCCAGGAAA
AGTGGGATTTGTATTGGGTAAAAATCATGTTCGATTAAACCCAAGCTACGTCCCATTGCAGTTGCTAACTCGAATGAATA
CGGTTTTCCCTAGCTACCAATGGGAAGAAATATATCAATCAAGCGCAAAATTACTAAAAGAAACCATGCCAAAAGGCTAT
AGTCCTGATTGGGTTGAGTGGGATAAAACTCAATTTAAAAAAGATTCAAAGACCCAAAGTGTTGGCAGCTATAATGCGAT
TAGAGTGTATTTATGGGCAGGAATGCTACCAGATAGCGACCCTAATAAAGCATTGTTACTTGATAAAATGAAGCCATTAG
TTCGTGTTATAGAAAGAAATAAAGGCATGCCTGAGACAATAAATGTGCTAACAGGAAAAGGTAAAAATCAAGGTGGCGTC
GGAATGAACGCAGCTATTTTGCCTTTATTATCTTCGTTAGATTCAAATACAAATGCCGTTGAATATGAGAAAAAGATCCA
AGCAGAATTGTCGAAGATTGAAAGTGATTATTACTATAACAGTGTGCTGACTTTATTCGGCCTTGGCTGGTATCAAGATT
TATACTCTTTTAATGATGATGGCAGCGTTACTCCTAAGTGGGTTAATGTATGTCAGTAA

Upstream 100 bases:

>100_bases
CTTTGCTTGCCGCCTTATTAGTTGTAATGGTCACAATTATTTTATTTAGAGTACTTCGCCGTATCGCCAAAAATCGTTTA
AACACAGAGGAAGATGAATA

Downstream 100 bases:

>100_bases
GCAAATACCGCCTTAAACAATTTGTTTCTTTTGCTATGCTGGGGGCTATGTATTCATCGCCAGTTTCTGCTGTATCATTG
AATCGAGATATCAATAACCA

Product: endo-1,4-D-glucanase

Products: NA

Alternate protein names: Carboxymethylcellulase; CMCase; Cellulase; Endo-1,4-beta-glucanase [H]

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MKMQLTVLSLLIGTLTCSSSAIAQDENYTTPIVSSAALTKENQCEWGQWESFKQHYIENGRVVDNSDPRLITTSEGQSYA
LFFALIANDKKTFDELLGWTELHLAGGDLTAQLPAWLWGRQPDGSQGILDSNSAADSDLWIAYSLLEAGRLWDNHYYQSL
GHLLASRILRDETIKVSGLGTVLLPGKVGFVLGKNHVRLNPSYVPLQLLTRMNTVFPSYQWEEIYQSSAKLLKETMPKGY
SPDWVEWDKTQFKKDSKTQSVGSYNAIRVYLWAGMLPDSDPNKALLLDKMKPLVRVIERNKGMPETINVLTGKGKNQGGV
GMNAAILPLLSSLDSNTNAVEYEKKIQAELSKIESDYYYNSVLTLFGLGWYQDLYSFNDDGSVTPKWVNVCQ

Sequences:

>Translated_392_residues
MKMQLTVLSLLIGTLTCSSSAIAQDENYTTPIVSSAALTKENQCEWGQWESFKQHYIENGRVVDNSDPRLITTSEGQSYA
LFFALIANDKKTFDELLGWTELHLAGGDLTAQLPAWLWGRQPDGSQGILDSNSAADSDLWIAYSLLEAGRLWDNHYYQSL
GHLLASRILRDETIKVSGLGTVLLPGKVGFVLGKNHVRLNPSYVPLQLLTRMNTVFPSYQWEEIYQSSAKLLKETMPKGY
SPDWVEWDKTQFKKDSKTQSVGSYNAIRVYLWAGMLPDSDPNKALLLDKMKPLVRVIERNKGMPETINVLTGKGKNQGGV
GMNAAILPLLSSLDSNTNAVEYEKKIQAELSKIESDYYYNSVLTLFGLGWYQDLYSFNDDGSVTPKWVNVCQ
>Mature_392_residues
MKMQLTVLSLLIGTLTCSSSAIAQDENYTTPIVSSAALTKENQCEWGQWESFKQHYIENGRVVDNSDPRLITTSEGQSYA
LFFALIANDKKTFDELLGWTELHLAGGDLTAQLPAWLWGRQPDGSQGILDSNSAADSDLWIAYSLLEAGRLWDNHYYQSL
GHLLASRILRDETIKVSGLGTVLLPGKVGFVLGKNHVRLNPSYVPLQLLTRMNTVFPSYQWEEIYQSSAKLLKETMPKGY
SPDWVEWDKTQFKKDSKTQSVGSYNAIRVYLWAGMLPDSDPNKALLLDKMKPLVRVIERNKGMPETINVLTGKGKNQGGV
GMNAAILPLLSSLDSNTNAVEYEKKIQAELSKIESDYYYNSVLTLFGLGWYQDLYSFNDDGSVTPKWVNVCQ

Specific function: Hydrolyzes carboxymethylcellulose [H]

COG id: COG3405

COG function: function code G; Endoglucanase Y

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 8 (cellulase D) family [H]

Homologues:

Organism=Escherichia coli, GI1789951, Length=361, Percent_Identity=46.5373961218837, Blast_Score=325, Evalue=3e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008928
- InterPro:   IPR012341
- InterPro:   IPR002037
- InterPro:   IPR019834 [H]

Pfam domain/function: PF01270 Glyco_hydro_8 [H]

EC number: =3.2.1.4 [H]

Molecular weight: Translated: 43919; Mature: 43919

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00589 PTS_HPR_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMQLTVLSLLIGTLTCSSSAIAQDENYTTPIVSSAALTKENQCEWGQWESFKQHYIENG
CCCHHHHHHHHHHHHHCCCCHHCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHCCC
RVVDNSDPRLITTSEGQSYALFFALIANDKKTFDELLGWTELHLAGGDLTAQLPAWLWGR
EEEECCCCCEEECCCCCCEEEEEEEECCCHHHHHHHCCCEEEEEECCCCCCCCHHHHCCC
QPDGSQGILDSNSAADSDLWIAYSLLEAGRLWDNHYYQSLGHLLASRILRDETIKVSGLG
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCC
TVLLPGKVGFVLGKNHVRLNPSYVPLQLLTRMNTVFPSYQWEEIYQSSAKLLKETMPKGY
EEEECCCEEEEECCCEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCC
SPDWVEWDKTQFKKDSKTQSVGSYNAIRVYLWAGMLPDSDPNKALLLDKMKPLVRVIERN
CCCCCCCCHHHHCCCCCHHCCCCCCEEEEEEEECCCCCCCCCCEEHHHHHHHHHHHHHHC
KGMPETINVLTGKGKNQGGVGMNAAILPLLSSLDSNTNAVEYEKKIQAELSKIESDYYYN
CCCCCEEHEEECCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
SVLTLFGLGWYQDLYSFNDDGSVTPKWVNVCQ
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCC
>Mature Secondary Structure
MKMQLTVLSLLIGTLTCSSSAIAQDENYTTPIVSSAALTKENQCEWGQWESFKQHYIENG
CCCHHHHHHHHHHHHHCCCCHHCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHCCC
RVVDNSDPRLITTSEGQSYALFFALIANDKKTFDELLGWTELHLAGGDLTAQLPAWLWGR
EEEECCCCCEEECCCCCCEEEEEEEECCCHHHHHHHCCCEEEEEECCCCCCCCHHHHCCC
QPDGSQGILDSNSAADSDLWIAYSLLEAGRLWDNHYYQSLGHLLASRILRDETIKVSGLG
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCC
TVLLPGKVGFVLGKNHVRLNPSYVPLQLLTRMNTVFPSYQWEEIYQSSAKLLKETMPKGY
EEEECCCEEEEECCCEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCC
SPDWVEWDKTQFKKDSKTQSVGSYNAIRVYLWAGMLPDSDPNKALLLDKMKPLVRVIERN
CCCCCCCCHHHHCCCCCHHCCCCCCEEEEEEEECCCCCCCCCCEEHHHHHHHHHHHHHHC
KGMPETINVLTGKGKNQGGVGMNAAILPLLSSLDSNTNAVEYEKKIQAELSKIESDYYYN
CCCCCEEHEEECCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
SVLTLFGLGWYQDLYSFNDDGSVTPKWVNVCQ
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]