Click here to switch to the map view.

The map label for this gene is malE [H]

Identifier: 59713982

GI number: 59713982

Start: 896261

End: 897445

Strand: Reverse

Name: malE [H]

Synonym: VF_A0799

Alternate gene names: 59713982

Gene position: 897445-896261 (Counterclockwise)

Preceding gene: 59713985

Following gene: 59713981

Centisome position: 67.46

GC content: 40.84

Gene sequence:

>1185_bases
ATGAAAAAAGTCCTCAGTACTGTCGCACTATGCACAATGGCAGCTCTTGGTTCTATTAACGCTTCTGCTGCAATTGAAGA
AGGTCAATTAACGATTTGGGTAACGGGTGATAAAGCTTACGAAGGTATGGCTGAAGTAGGTAAACGTTTTGAAGAAGATA
CAGGAATTAAAGTAACTGTATCGTTCCCAGACAAAGCAGAAGAAAAATTTGCACAAGTAGCAGCTGCAGGCGATGGTCCT
GACATGATTTTTTACGCACACGACCGTTTTGGTGGTTTTGCGGAAGCGGGCCTATTAGCGGAAATTAAACCTTCAGAAGA
GTTGAAGTCTGAAATCGTTGATTTTGCATGGGATGCAGTAGAATACAAAGGAAAAACAATTGCTTACCCTGTAGCTATTG
AATCTGTATCTCTTATTTACAATAAAGCTCTTATCAAAAATCCACCTAAAAACTGGGAAGAAATCCCTGCTTTAGATAAG
GAAATGATGAAGAATGGCCAAAAAGCGATCATGTGGCCACTTCGTGGTGGGGCTTATTTCACTTGGCCTTTATTAGCAGC
AGATGGCGGATATGCATTTAAGAAAACCGCTGAAGGTTACGACATTAAAAACGCTGGTGTAAACCAAAAAGGCGTTCAAG
ATGCAATGACGTTCATCGAAACTATGGTGAAAGACAAAACCATTTCGGCAGATATGGATTACTCAGTTGCTGAGTCTGAA
TTTGTTAAAGGCAATGTGGGTATGATGATTAACGGTCCTTGGGGCTGGGCAAACATCGAAAAAGCGGGCATTGATTATGG
AGTAACGACACTGCCTAAATTCCATGGTCAGGCATCTAAACCATTCGTTGGTGTATGGGTTGGTGGTATCAGCGCAGTAT
CTCCAAACAAAGATTTAGCCGTTGAGTTTTTTGAAAGTTACTTGCTAACTGATGAAGGTCTGAAAAGCTTAAATGACGAT
AAACCTTTAGGTGCTGTAGCTTTAAATTCATTCCAAAAGCAACTTGATACCGATTCTCGTATTGCTGCGACAATGAATAA
TGCAATGAATGGTGAAATCATGCCGAACATTCCTCAATTCACTACTTTCTGGTACAGCATGGAAGAAGCATTAGGTAACG
TTGTTGACGGTCGTCAAATGGTGACTGATGCACTAGCTGCTGCTGAAAAACGCATGACTAAATAA

Upstream 100 bases:

>100_bases
AGTGATGAATTGATGAAAATACACCTTGAGAATTGAATCATCATCTACTTTAACTTCAACCCTACCGATGATTCATTAAT
TATAAAAGGAAGGATTCACA

Downstream 100 bases:

>100_bases
CACCTTATTAGTCATACAATTAGGAGGGGAGGTTTACTTCTCTCCTAATAATAGCGAATCATCTCTTCTTTAATATCCAC
ATGAGTAAGCCTATTTTATC

Product: maltose ABC transporter periplasmic protein

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: MMBP; Maltodextrin-binding protein [H]

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MKKVLSTVALCTMAALGSINASAAIEEGQLTIWVTGDKAYEGMAEVGKRFEEDTGIKVTVSFPDKAEEKFAQVAAAGDGP
DMIFYAHDRFGGFAEAGLLAEIKPSEELKSEIVDFAWDAVEYKGKTIAYPVAIESVSLIYNKALIKNPPKNWEEIPALDK
EMMKNGQKAIMWPLRGGAYFTWPLLAADGGYAFKKTAEGYDIKNAGVNQKGVQDAMTFIETMVKDKTISADMDYSVAESE
FVKGNVGMMINGPWGWANIEKAGIDYGVTTLPKFHGQASKPFVGVWVGGISAVSPNKDLAVEFFESYLLTDEGLKSLNDD
KPLGAVALNSFQKQLDTDSRIAATMNNAMNGEIMPNIPQFTTFWYSMEEALGNVVDGRQMVTDALAAAEKRMTK

Sequences:

>Translated_394_residues
MKKVLSTVALCTMAALGSINASAAIEEGQLTIWVTGDKAYEGMAEVGKRFEEDTGIKVTVSFPDKAEEKFAQVAAAGDGP
DMIFYAHDRFGGFAEAGLLAEIKPSEELKSEIVDFAWDAVEYKGKTIAYPVAIESVSLIYNKALIKNPPKNWEEIPALDK
EMMKNGQKAIMWPLRGGAYFTWPLLAADGGYAFKKTAEGYDIKNAGVNQKGVQDAMTFIETMVKDKTISADMDYSVAESE
FVKGNVGMMINGPWGWANIEKAGIDYGVTTLPKFHGQASKPFVGVWVGGISAVSPNKDLAVEFFESYLLTDEGLKSLNDD
KPLGAVALNSFQKQLDTDSRIAATMNNAMNGEIMPNIPQFTTFWYSMEEALGNVVDGRQMVTDALAAAEKRMTK
>Mature_394_residues
MKKVLSTVALCTMAALGSINASAAIEEGQLTIWVTGDKAYEGMAEVGKRFEEDTGIKVTVSFPDKAEEKFAQVAAAGDGP
DMIFYAHDRFGGFAEAGLLAEIKPSEELKSEIVDFAWDAVEYKGKTIAYPVAIESVSLIYNKALIKNPPKNWEEIPALDK
EMMKNGQKAIMWPLRGGAYFTWPLLAADGGYAFKKTAEGYDIKNAGVNQKGVQDAMTFIETMVKDKTISADMDYSVAESE
FVKGNVGMMINGPWGWANIEKAGIDYGVTTLPKFHGQASKPFVGVWVGGISAVSPNKDLAVEFFESYLLTDEGLKSLNDD
KPLGAVALNSFQKQLDTDSRIAATMNNAMNGEIMPNIPQFTTFWYSMEEALGNVVDGRQMVTDALAAAEKRMTK

Specific function: Involved in the high-affinity maltose membrane transport system malEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides [H]

COG id: COG2182

COG function: function code G; Maltose-binding periplasmic proteins/domains

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790466, Length=392, Percent_Identity=59.4387755102041, Blast_Score=487, Evalue=1e-139,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 720 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006060
- InterPro:   IPR006059
- InterPro:   IPR006061 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 42913; Mature: 42913

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS01037 SBP_BACTERIAL_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVLSTVALCTMAALGSINASAAIEEGQLTIWVTGDKAYEGMAEVGKRFEEDTGIKVTV
CHHHHHHHHHHHHHHHCCCCCCCEEECCCEEEEEECCHHHHHHHHHHHHHCCCCCEEEEE
SFPDKAEEKFAQVAAAGDGPDMIFYAHDRFGGFAEAGLLAEIKPSEELKSEIVDFAWDAV
ECCCHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHCCEEEECCCHHHHHHHHHHHHHHHH
EYKGKTIAYPVAIESVSLIYNKALIKNPPKNWEEIPALDKEMMKNGQKAIMWPLRGGAYF
HHCCCEEEEEEHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHCCCEEEEEEECCCCEE
TWPLLAADGGYAFKKTAEGYDIKNAGVNQKGVQDAMTFIETMVKDKTISADMDYSVAESE
EEEEEECCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
FVKGNVGMMINGPWGWANIEKAGIDYGVTTLPKFHGQASKPFVGVWVGGISAVSPNKDLA
HEECCCCEEEECCCCCCCCHHCCCCCCCCCCHHHCCCCCCCEEEEEECCEEECCCCCHHH
VEFFESYLLTDEGLKSLNDDKPLGAVALNSFQKQLDTDSRIAATMNNAMNGEIMPNIPQF
HHHHHHHHHCHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCHH
TTFWYSMEEALGNVVDGRQMVTDALAAAEKRMTK
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKVLSTVALCTMAALGSINASAAIEEGQLTIWVTGDKAYEGMAEVGKRFEEDTGIKVTV
CHHHHHHHHHHHHHHHCCCCCCCEEECCCEEEEEECCHHHHHHHHHHHHHCCCCCEEEEE
SFPDKAEEKFAQVAAAGDGPDMIFYAHDRFGGFAEAGLLAEIKPSEELKSEIVDFAWDAV
ECCCHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHCCEEEECCCHHHHHHHHHHHHHHHH
EYKGKTIAYPVAIESVSLIYNKALIKNPPKNWEEIPALDKEMMKNGQKAIMWPLRGGAYF
HHCCCEEEEEEHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHCCCEEEEEEECCCCEE
TWPLLAADGGYAFKKTAEGYDIKNAGVNQKGVQDAMTFIETMVKDKTISADMDYSVAESE
EEEEEECCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
FVKGNVGMMINGPWGWANIEKAGIDYGVTTLPKFHGQASKPFVGVWVGGISAVSPNKDLA
HEECCCCEEEECCCCCCCCHHCCCCCCCCCCHHHCCCCCCCEEEEEECCEEECCCCCHHH
VEFFESYLLTDEGLKSLNDDKPLGAVALNSFQKQLDTDSRIAATMNNAMNGEIMPNIPQF
HHHHHHHHHCHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCHH
TTFWYSMEEALGNVVDGRQMVTDALAAAEKRMTK
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]