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The map label for this gene is ebgR [H]

Identifier: 59713531

GI number: 59713531

Start: 388632

End: 389627

Strand: Reverse

Name: ebgR [H]

Synonym: VF_A0348

Alternate gene names: 59713531

Gene position: 389627-388632 (Counterclockwise)

Preceding gene: 59713532

Following gene: 59713530

Centisome position: 29.29

GC content: 37.15

Gene sequence:

>996_bases
ATGGCAACGTTAAAGGAAATAGCCAACGAAGCTGAAGTTTCATTGGCAACGGTTTCTCGTGTCTTGAATGAAGACCCGAC
GCTAAGCGTAAAAGAAGAAACAAAGCGACGTATTTTTGAAATAGCTGAAAAGCTTGAATATAAAACAAGCTCGACTCGCA
AGTTAGCCAGTGCATTACCCAAAATAGCAAAAGAGCAATTACACTTTCTTGCTCTATACAATTACAAGCAAGAGACCGAA
GTAAACGACCCATATTACCTTGCTATTCGCCATGGTATTGAAACCCAGTGTGATAAGTTTGATATTACATTAACCAATAG
TTATGAAAGCAGCTTAGACATCAAGAACTCAAAAGTTGATGGTATTTTACTGGTTGGTCGTCAAACTAAAGAACAATTAA
AAGCGATTCAAAAAATCAGCAAAAACATTGTTTATATTGATTATACCGATCATGAGCAAAACTTTGATTCTGTCGATATC
GATCTTGCTCGTATCAGTAAAGAAGTAATTAACTACTTCATGAATCAAGGTTATCAACGTATTGGTTTTATTGGTGGTCA
AGATGATGAAAACTGTTCAGACATTCGTGAAGAAGTATTCGCTGAATATGGTCAACTAAAAGGCGTGGTATCCGAATCGG
ATCTCTATCGTGGTGATTTCTCTAGTTCATCGGGTTATAAGCTTGCAAAAGAGATGCTAGAAACAGATTACCCAAATGCG
TGGTTTATTGCCTCAGATTCGATTGCTATTGGCGTTTTACGTGCTATCCATGAAAAAGGATTACGTATCCCTGAAGACAT
TGCTTTAATCAGTGTTAATGATATTCCAACAGCTAAATTTACTTTCCCTTCTTTATCAACGGTTCGAATCCACTCTGAAC
TAATGGGAAGCCAAGGCATTAACTTATTACTTGAACGTCGTCGTGATGAACGTGCGATTCCAGTACGTGTTTATATTCCA
AGTAAATTACGCTTACGTGATACGACTAAAAAGTAA

Upstream 100 bases:

>100_bases
AACCACAGTAAAACTTTAGTAAAAGCCATTTTTAAGTATCAAAATCATCATTTATTAAGTAACATGGAGCAATATTATAA
AATTAAAGGCTGGAGGATGA

Downstream 100 bases:

>100_bases
AATATTCCAATAAGTTTACTACCTACATTAAGCAACGTTTATGGCGTTGCTTTTTTTATATTAATAACTCCCCCCCTTTA
CTCCTTCTATCCTTTTCGAA

Product: DNA-binding transcriptional repressor EbgR

Products: NA

Alternate protein names: Ebg operon repressor [H]

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MATLKEIANEAEVSLATVSRVLNEDPTLSVKEETKRRIFEIAEKLEYKTSSTRKLASALPKIAKEQLHFLALYNYKQETE
VNDPYYLAIRHGIETQCDKFDITLTNSYESSLDIKNSKVDGILLVGRQTKEQLKAIQKISKNIVYIDYTDHEQNFDSVDI
DLARISKEVINYFMNQGYQRIGFIGGQDDENCSDIREEVFAEYGQLKGVVSESDLYRGDFSSSSGYKLAKEMLETDYPNA
WFIASDSIAIGVLRAIHEKGLRIPEDIALISVNDIPTAKFTFPSLSTVRIHSELMGSQGINLLLERRRDERAIPVRVYIP
SKLRLRDTTKK

Sequences:

>Translated_331_residues
MATLKEIANEAEVSLATVSRVLNEDPTLSVKEETKRRIFEIAEKLEYKTSSTRKLASALPKIAKEQLHFLALYNYKQETE
VNDPYYLAIRHGIETQCDKFDITLTNSYESSLDIKNSKVDGILLVGRQTKEQLKAIQKISKNIVYIDYTDHEQNFDSVDI
DLARISKEVINYFMNQGYQRIGFIGGQDDENCSDIREEVFAEYGQLKGVVSESDLYRGDFSSSSGYKLAKEMLETDYPNA
WFIASDSIAIGVLRAIHEKGLRIPEDIALISVNDIPTAKFTFPSLSTVRIHSELMGSQGINLLLERRRDERAIPVRVYIP
SKLRLRDTTKK
>Mature_330_residues
ATLKEIANEAEVSLATVSRVLNEDPTLSVKEETKRRIFEIAEKLEYKTSSTRKLASALPKIAKEQLHFLALYNYKQETEV
NDPYYLAIRHGIETQCDKFDITLTNSYESSLDIKNSKVDGILLVGRQTKEQLKAIQKISKNIVYIDYTDHEQNFDSVDID
LARISKEVINYFMNQGYQRIGFIGGQDDENCSDIREEVFAEYGQLKGVVSESDLYRGDFSSSSGYKLAKEMLETDYPNAW
FIASDSIAIGVLRAIHEKGLRIPEDIALISVNDIPTAKFTFPSLSTVRIHSELMGSQGINLLLERRRDERAIPVRVYIPS
KLRLRDTTKK

Specific function: Repressor for beta galactosidase alpha and beta subunits (ebgA and ebgC). Binds lactose as an inducer [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789456, Length=333, Percent_Identity=66.3663663663664, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI1787948, Length=340, Percent_Identity=28.2352941176471, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1790194, Length=319, Percent_Identity=26.0188087774295, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1790369, Length=351, Percent_Identity=25.6410256410256, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1789068, Length=321, Percent_Identity=27.1028037383178, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1786540, Length=344, Percent_Identity=22.9651162790698, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787580, Length=338, Percent_Identity=23.3727810650888, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788474, Length=345, Percent_Identity=22.8985507246377, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI48994940, Length=324, Percent_Identity=22.5308641975309, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37708; Mature: 37577

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLKEIANEAEVSLATVSRVLNEDPTLSVKEETKRRIFEIAEKLEYKTSSTRKLASALP
CCCHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
KIAKEQLHFLALYNYKQETEVNDPYYLAIRHGIETQCDKFDITLTNSYESSLDIKNSKVD
HHHHHHHHHHHEECCCCCCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCC
GILLVGRQTKEQLKAIQKISKNIVYIDYTDHEQNFDSVDIDLARISKEVINYFMNQGYQR
EEEEECCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCE
IGFIGGQDDENCSDIREEVFAEYGQLKGVVSESDLYRGDFSSSSGYKLAKEMLETDYPNA
EECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCE
WFIASDSIAIGVLRAIHEKGLRIPEDIALISVNDIPTAKFTFPSLSTVRIHSELMGSQGI
EEEECCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCEEECCCCCEEEHHHHHHCCCCH
NLLLERRRDERAIPVRVYIPSKLRLRDTTKK
HHHHHHCCCCCCCEEEEEECCCEEECCCCCC
>Mature Secondary Structure 
ATLKEIANEAEVSLATVSRVLNEDPTLSVKEETKRRIFEIAEKLEYKTSSTRKLASALP
CCHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
KIAKEQLHFLALYNYKQETEVNDPYYLAIRHGIETQCDKFDITLTNSYESSLDIKNSKVD
HHHHHHHHHHHEECCCCCCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCC
GILLVGRQTKEQLKAIQKISKNIVYIDYTDHEQNFDSVDIDLARISKEVINYFMNQGYQR
EEEEECCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCE
IGFIGGQDDENCSDIREEVFAEYGQLKGVVSESDLYRGDFSSSSGYKLAKEMLETDYPNA
EECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCE
WFIASDSIAIGVLRAIHEKGLRIPEDIALISVNDIPTAKFTFPSLSTVRIHSELMGSQGI
EEEECCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCEEECCCCCEEEHHHHHHCCCCH
NLLLERRRDERAIPVRVYIPSKLRLRDTTKK
HHHHHHCCCCCCCEEEEEECCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2515108; 3939708; 9278503 [H]