Click here to switch to the map view.

The map label for this gene is btuB [H]

Identifier: 59713515

GI number: 59713515

Start: 366823

End: 368958

Strand: Reverse

Name: btuB [H]

Synonym: VF_A0332

Alternate gene names: 59713515

Gene position: 368958-366823 (Counterclockwise)

Preceding gene: 59713516

Following gene: 59713514

Centisome position: 27.73

GC content: 37.92

Gene sequence:

>2136_bases
ATGAAGCTTTCACCAATAACAGCGGCAGTACTTAGCGTACTGACTGCCAACTTCGTACACGCTAATTCTGAGGTTACACA
ATTTGATGAAGTTGTCGTTACAGCTAACAAAGTAGAACAACCCTTATCAAAAGTTGCAGGGTCTATTACTGTTATCAATG
GCGATGACCTAGAAACAAAAGGAACAACAGAATTATACGACTCTCTCAATAAAGAAGCAGGAGTCAGTGTTACTGGTGGC
GCTGGTCGCCCGCAAAATATAACAATTCGAGGAATGACAGGGAATCGTATTGTTATTGTAAAAGACGGAATTAAATCTTC
CGATGGTTACGGCGCTTCAGATATTAATGACAAAGTTGGCCGTAATTCGTTTGATCTATCAAACGTAAAATCAATTGAAG
TTGTAAAAGGTGCCAGCTCTTCAGTTCATGGTTCAGGCGCTATTGGTGGTGTTGTTATTATTGAAACGAAACAACCTGAA
GACTATTTAAAAGGTAACGATTTCTATTCTGATGTTTCTGGTACTTACACAGGCATAAGTAATAAATATAAAGGTAGCAG
TAACCTAGCGTTTCGCTCTGGAAATACTGAAAGTCTAATTAATTTATCCTACTGGCAAGGTGAAGAAACCCGTAACTACC
AACAAGATCTATATAACCGTAATGTTGATGGTGTAAACGCTGCATATACAGTAAATCACTACATTGGCGAAGAGCTAATG
CTAACCGCTAAAATTGAATACTACACCGAAGATATGTTACGTGAAGAAGGATCCGCATCTATTCAAAAAGATGGTATCTG
GCATATATCTACCTATAACCACAAAGAAAATACTCAAGATATCAACGCTTATATTGGCGCTGAATATACACCTTACGATG
CTTTCTTTGAGAAATTAAATTCAAAACTCTACTGGAGAGATACCACAACATCTTCAGACATCAATAGATTAATGTCGCGT
GAGCGAAATAATATTGTAGAAAAACGCCGAGAAATAAAAGCTCAAAGTTTTACTGATCAATTAATTGGTTTTGATAACGA
TTTTATAAGTTCTGCTACTCTATTAAACCAAGTTCATAATCTTGCTTATGGTCTAAGTTTAACAAGCAATCAATATGAGA
GACCAAACAGCAAATCAACTATTGATTGGAACGGTAATAATGTAAATCACACACAACCGTTCGCACCAGCAAGAGCATAT
AGTCTTGGCATTTATTTGCGCGATATGGTGGAAGTTAACAATTGGATTATCACTGGAGGGCTTCGTTTTGATGCGCACAG
CTTAACCCCTAGCGATTCTGACTCTATTGCTGGTTTTAGTGTAAAAAAAATAAATAGCAGTGAGCTATCTCCTAGTGTTT
CTGCGACCTATCAATTTACAGATACACTCAACGCGTATTTATCCTATAACCACGGTTATCGTGCTCCAGCTTATGATAAA
GCATACGGATATAACAACCATGACTTTGTTCCTCTGACCCCATTCTTAATCATTCCTAATATGGATTTAGAAGCAGAGAC
AAGTGATTCTATCGAACACGGTATTAAATATGATTCTGGTAAAACTCAAATCTATGGTGCTGTTTTTTATCAAAAATTTG
AAAACTTTATCGATGTTATACAAGTTGGAATGGACCCATCTACCGGGAATTACTTAAAGCAATACCAAAACATCAGTGGT
GTCGAGACTTATGGAGCTGAATTAACCCTAGCACACCAATTCACCCAAGAATGGGGAATAAACTCAAAGATTGGTTATGT
TGACGGAAAAGATGGCGATGGTGAATACATTCGCACCATCACTCCATTAGAAGGAAATGTTGGTATTACTTACGATCTTG
AAAAACTGTCAGCCTACAGCTCTCTTAATTGGGCATCCTCAATGGATCGAGTACCACAATGCGCAACGAATATCGGTATG
GCAACAGAATGTGCGACAACATCAAGCTGGGCAACGGTTGATCTTGGCGTAAATTACGCTGTCACCACAGATTTTAATAT
CAGCGCAAACATCATCAATCTATTCGATAAAGAATACATCCGCTATCAAGATGTTGCAGGAATTATGGACTCAGCGAAAG
GCTATTCCACTGAACCTGGACGTTATTTCACTGTAAACGCTAAATACGTATTTTAA

Upstream 100 bases:

>100_bases
CAAGGACACTCAACAAATAGACGGGATGCTTTAAAACAACAAGCGTTTGAATATATTTTTCTTTTATCTCTAATAAATAA
TTAAAAAAGCGGAGCTACAA

Downstream 100 bases:

>100_bases
GAGATAAAAATGATTAAGTACCTTTATTACTTCATGTTTGGATTATGGTTTTCCACTCAAACCCTTGCTGGTGAGTGGGA
TTATCCAGATCAATTAATAA

Product: TonB system receptor, putative

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 711; Mature: 711

Protein sequence:

>711_residues
MKLSPITAAVLSVLTANFVHANSEVTQFDEVVVTANKVEQPLSKVAGSITVINGDDLETKGTTELYDSLNKEAGVSVTGG
AGRPQNITIRGMTGNRIVIVKDGIKSSDGYGASDINDKVGRNSFDLSNVKSIEVVKGASSSVHGSGAIGGVVIIETKQPE
DYLKGNDFYSDVSGTYTGISNKYKGSSNLAFRSGNTESLINLSYWQGEETRNYQQDLYNRNVDGVNAAYTVNHYIGEELM
LTAKIEYYTEDMLREEGSASIQKDGIWHISTYNHKENTQDINAYIGAEYTPYDAFFEKLNSKLYWRDTTTSSDINRLMSR
ERNNIVEKRREIKAQSFTDQLIGFDNDFISSATLLNQVHNLAYGLSLTSNQYERPNSKSTIDWNGNNVNHTQPFAPARAY
SLGIYLRDMVEVNNWIITGGLRFDAHSLTPSDSDSIAGFSVKKINSSELSPSVSATYQFTDTLNAYLSYNHGYRAPAYDK
AYGYNNHDFVPLTPFLIIPNMDLEAETSDSIEHGIKYDSGKTQIYGAVFYQKFENFIDVIQVGMDPSTGNYLKQYQNISG
VETYGAELTLAHQFTQEWGINSKIGYVDGKDGDGEYIRTITPLEGNVGITYDLEKLSAYSSLNWASSMDRVPQCATNIGM
ATECATTSSWATVDLGVNYAVTTDFNISANIINLFDKEYIRYQDVAGIMDSAKGYSTEPGRYFTVNAKYVF

Sequences:

>Translated_711_residues
MKLSPITAAVLSVLTANFVHANSEVTQFDEVVVTANKVEQPLSKVAGSITVINGDDLETKGTTELYDSLNKEAGVSVTGG
AGRPQNITIRGMTGNRIVIVKDGIKSSDGYGASDINDKVGRNSFDLSNVKSIEVVKGASSSVHGSGAIGGVVIIETKQPE
DYLKGNDFYSDVSGTYTGISNKYKGSSNLAFRSGNTESLINLSYWQGEETRNYQQDLYNRNVDGVNAAYTVNHYIGEELM
LTAKIEYYTEDMLREEGSASIQKDGIWHISTYNHKENTQDINAYIGAEYTPYDAFFEKLNSKLYWRDTTTSSDINRLMSR
ERNNIVEKRREIKAQSFTDQLIGFDNDFISSATLLNQVHNLAYGLSLTSNQYERPNSKSTIDWNGNNVNHTQPFAPARAY
SLGIYLRDMVEVNNWIITGGLRFDAHSLTPSDSDSIAGFSVKKINSSELSPSVSATYQFTDTLNAYLSYNHGYRAPAYDK
AYGYNNHDFVPLTPFLIIPNMDLEAETSDSIEHGIKYDSGKTQIYGAVFYQKFENFIDVIQVGMDPSTGNYLKQYQNISG
VETYGAELTLAHQFTQEWGINSKIGYVDGKDGDGEYIRTITPLEGNVGITYDLEKLSAYSSLNWASSMDRVPQCATNIGM
ATECATTSSWATVDLGVNYAVTTDFNISANIINLFDKEYIRYQDVAGIMDSAKGYSTEPGRYFTVNAKYVF
>Mature_711_residues
MKLSPITAAVLSVLTANFVHANSEVTQFDEVVVTANKVEQPLSKVAGSITVINGDDLETKGTTELYDSLNKEAGVSVTGG
AGRPQNITIRGMTGNRIVIVKDGIKSSDGYGASDINDKVGRNSFDLSNVKSIEVVKGASSSVHGSGAIGGVVIIETKQPE
DYLKGNDFYSDVSGTYTGISNKYKGSSNLAFRSGNTESLINLSYWQGEETRNYQQDLYNRNVDGVNAAYTVNHYIGEELM
LTAKIEYYTEDMLREEGSASIQKDGIWHISTYNHKENTQDINAYIGAEYTPYDAFFEKLNSKLYWRDTTTSSDINRLMSR
ERNNIVEKRREIKAQSFTDQLIGFDNDFISSATLLNQVHNLAYGLSLTSNQYERPNSKSTIDWNGNNVNHTQPFAPARAY
SLGIYLRDMVEVNNWIITGGLRFDAHSLTPSDSDSIAGFSVKKINSSELSPSVSATYQFTDTLNAYLSYNHGYRAPAYDK
AYGYNNHDFVPLTPFLIIPNMDLEAETSDSIEHGIKYDSGKTQIYGAVFYQKFENFIDVIQVGMDPSTGNYLKQYQNISG
VETYGAELTLAHQFTQEWGINSKIGYVDGKDGDGEYIRTITPLEGNVGITYDLEKLSAYSSLNWASSMDRVPQCATNIGM
ATECATTSSWATVDLGVNYAVTTDFNISANIINLFDKEYIRYQDVAGIMDSAKGYSTEPGRYFTVNAKYVF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG1629

COG function: function code P; Outer membrane receptor proteins, mostly Fe transport

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=155, Percent_Identity=32.258064516129, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI1788478, Length=133, Percent_Identity=36.8421052631579, Blast_Score=73, Evalue=6e-14,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010916
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 78739; Mature: 78739

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS01156 TONB_DEPENDENT_REC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSPITAAVLSVLTANFVHANSEVTQFDEVVVTANKVEQPLSKVAGSITVINGDDLETK
CCCCHHHHHHHHHHHHHHHCCCCCHHHHHHEEEEHHHHHHHHHHHCCEEEEEECCCCCCC
GTTELYDSLNKEAGVSVTGGAGRPQNITIRGMTGNRIVIVKDGIKSSDGYGASDINDKVG
CCHHHHHHCCCCCCCEEECCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCCHHHC
RNSFDLSNVKSIEVVKGASSSVHGSGAIGGVVIIETKQPEDYLKGNDFYSDVSGTYTGIS
CCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCHHHHCCCCCCCCCCCCCCCCC
NKYKGSSNLAFRSGNTESLINLSYWQGEETRNYQQDLYNRNVDGVNAAYTVNHYIGEELM
CCCCCCCCEEEECCCCCCEEEEEEECCCCCCHHHHHHHCCCCCCCCEEEEEHHHCCCEEE
LTAKIEYYTEDMLREEGSASIQKDGIWHISTYNHKENTQDINAYIGAEYTPYDAFFEKLN
EEEEEHHHHHHHHHCCCCCCEECCCEEEEEECCCCCCCCCCHHEECCCCCCHHHHHHHHC
SKLYWRDTTTSSDINRLMSRERNNIVEKRREIKAQSFTDQLIGFDNDFISSATLLNQVHN
CEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LAYGLSLTSNQYERPNSKSTIDWNGNNVNHTQPFAPARAYSLGIYLRDMVEVNNWIITGG
HHEEEEECCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHEEEHHHHHHHHCCCCEEEECC
LRFDAHSLTPSDSDSIAGFSVKKINSSELSPSVSATYQFTDTLNAYLSYNHGYRAPAYDK
EEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCC
AYGYNNHDFVPLTPFLIIPNMDLEAETSDSIEHGIKYDSGKTQIYGAVFYQKFENFIDVI
CCCCCCCCCEECCCEEEECCCCCCCCCCCHHHHCCEECCCCCEEEHHHHHHHHHHHHHHH
QVGMDPSTGNYLKQYQNISGVETYGAELTLAHQFTQEWGINSKIGYVDGKDGDGEYIRTI
HHCCCCCCCHHHHHHCCCCCCCCCCCEEEEHHHHHHHCCCCCCEEEECCCCCCCCEEEEE
TPLEGNVGITYDLEKLSAYSSLNWASSMDRVPQCATNIGMATECATTSSWATVDLGVNYA
ECCCCCEEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEECCCCCCCEEEEECCEEE
VTTDFNISANIINLFDKEYIRYQDVAGIMDSAKGYSTEPGRYFTVNAKYVF
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEC
>Mature Secondary Structure
MKLSPITAAVLSVLTANFVHANSEVTQFDEVVVTANKVEQPLSKVAGSITVINGDDLETK
CCCCHHHHHHHHHHHHHHHCCCCCHHHHHHEEEEHHHHHHHHHHHCCEEEEEECCCCCCC
GTTELYDSLNKEAGVSVTGGAGRPQNITIRGMTGNRIVIVKDGIKSSDGYGASDINDKVG
CCHHHHHHCCCCCCCEEECCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCCHHHC
RNSFDLSNVKSIEVVKGASSSVHGSGAIGGVVIIETKQPEDYLKGNDFYSDVSGTYTGIS
CCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCHHHHCCCCCCCCCCCCCCCCC
NKYKGSSNLAFRSGNTESLINLSYWQGEETRNYQQDLYNRNVDGVNAAYTVNHYIGEELM
CCCCCCCCEEEECCCCCCEEEEEEECCCCCCHHHHHHHCCCCCCCCEEEEEHHHCCCEEE
LTAKIEYYTEDMLREEGSASIQKDGIWHISTYNHKENTQDINAYIGAEYTPYDAFFEKLN
EEEEEHHHHHHHHHCCCCCCEECCCEEEEEECCCCCCCCCCHHEECCCCCCHHHHHHHHC
SKLYWRDTTTSSDINRLMSRERNNIVEKRREIKAQSFTDQLIGFDNDFISSATLLNQVHN
CEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LAYGLSLTSNQYERPNSKSTIDWNGNNVNHTQPFAPARAYSLGIYLRDMVEVNNWIITGG
HHEEEEECCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHEEEHHHHHHHHCCCCEEEECC
LRFDAHSLTPSDSDSIAGFSVKKINSSELSPSVSATYQFTDTLNAYLSYNHGYRAPAYDK
EEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCC
AYGYNNHDFVPLTPFLIIPNMDLEAETSDSIEHGIKYDSGKTQIYGAVFYQKFENFIDVI
CCCCCCCCCEECCCEEEECCCCCCCCCCCHHHHCCEECCCCCEEEHHHHHHHHHHHHHHH
QVGMDPSTGNYLKQYQNISGVETYGAELTLAHQFTQEWGINSKIGYVDGKDGDGEYIRTI
HHCCCCCCCHHHHHHCCCCCCCCCCCEEEEHHHHHHHCCCCCCEEEECCCCCCCCEEEEE
TPLEGNVGITYDLEKLSAYSSLNWASSMDRVPQCATNIGMATECATTSSWATVDLGVNYA
ECCCCCEEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEECCCCCCCEEEEECCEEE
VTTDFNISANIINLFDKEYIRYQDVAGIMDSAKGYSTEPGRYFTVNAKYVF
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA