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The map label for this gene is gacS [H]

Identifier: 59713416

GI number: 59713416

Start: 255417

End: 257627

Strand: Reverse

Name: gacS [H]

Synonym: VF_A0233

Alternate gene names: 59713416

Gene position: 257627-255417 (Counterclockwise)

Preceding gene: 59713417

Following gene: 59713397

Centisome position: 19.37

GC content: 28.54

Gene sequence:

>2211_bases
ATGCTTTTGAAAAACAAATTCTTTGTTGCTCTTGCTGGTACCATTATTGCCTATTCCGTCTTCATTTCGTTTACGTCGTA
TAAAATAATCACTTCAAATCAAGAAGAAGTAAAAACAATTGCTACAAATGCAGAAAATAAGTACTTACATCAAAGTGCAC
TGCACTATTCATTTGATGTAAGCAACTCATTAAATCGAAAAATGATTGAAATAAAAAACATTTCCAAAAGATATGTTGAG
TTAATCAAGAACATTCGATCAATTAAATCAAGAGATATCTTAAACACTGCTGTGCAAGAACTAAAAAAGTACTCTACTAG
CGTAATCATCAGTGATTCTAAGATCAGTTACACTACCGATGAAACACTCGTTACTCAACAATATAAAATCAATGAAAGTA
CATATTTAATATTTCAAACAGACGTAACTAAACTACTTAATAGATTAAAAAATAAAGACTATTACGCTGACTTCGTTACA
ATAATTATAGACAAAAATATGAATGTTGTGACTGATAATTCAGATAGTAAACAGTACTTATCACTATATAAACAAAAACT
GAAGGACATTACAAAGCTAGACGAAATTTCTTATGACTTAATGATAGCAATGAGAAAAATCGAAAAGAAAGAGACATGGG
ATTCAGAAATAAAAATAAATGGTGAAGACCATATTATTGCAATTCAACCCGTAAAAGACCTTCCATGGAGCATAGTTACT
CTTATAAAGAAAAAACTATCGGTCAATTACGATCAAGATCTAACGAATAAAATTGATAATAACTACATTCTTTTTAGTAA
TAAATTATTTAACACAATAATATTTATTACCCTATTCACTCTTATAACATCAATTTTAATCACTTATTATGTAAAAGGTC
CATTAATTCTGTTTAGTGAATGGATCAAAGAAATTCAAAGAGGTAATTATGAATACAATAATCCTTTACTATATAGAAAA
GACGAATTAGGAACATTAGCGAGAAATGTCTCTTTGATGGCCCAAGATATCTCGATTTTAGTCAATAACTTAGAAAGTAA
GATAACTGAAAGAACGAAACTTCTTCAACAATCCAAAGAGCAAGCAGAAAAAGCTAATATCAATAAATCAAAATTTATAG
CTAATATATCGCATGAATTAAGAACGCCTTTAAATGCGATATTAGGTCTAAGTTTATATCTTATCGAAAAAGAAGAAAAC
AGTGAGAAAAAGAAAACATTAGAAACATTAAATAAAGCGGGTCAAGGCTTATTAGAGATGGTTAATGACATTCTCGATTT
AAGTAAAATTGAAGCCGACAAAATCATTGCCCACAATAAACCAAACTCTATCGATCATATCATTTCAGACATTATTGATA
TTACTCAGTTTCAATCAAAGTCAAAGAATATCCAAATTCAATACAAAAAAACGGAATTTCACCATTTAAACATAGATAAA
AAATATATAAATAAGATAATAATGAATATCTTAAGTAATGCCATCAAGTTTACAGAAGATGGTGGAAAAATAGATATTGA
TGTAAAATATGAAAATTCCACACCAACAACAACCGATCTTATCATGGTATTTACCGATAATGGCCGAGGAATTGAAGAAA
AAGATTTAGAACGTATATTTAATCCATTTGAACAACTCTATGATAAAGACAGAGAATATGGAACAGGCCTTGGTTTGTTT
ATTACTAAGAAAATCATAGAAGATATGAACGGTAATATCTCAATTAAAAGTAAAGTTGGAATAGGAACATCTATTTGTAT
TAAATTAAAGGATGTTGAAATCATTAAATCTAAATTAATCGACCAATATCATACAATGCGGAGAAATGTTACTAAATTTA
AAGATTCAAAAACCATCGCAATTATTGATGATATAGAATTCAACAGGGAAGTTTTAAAGAAAAAGGTAGAAGATTACGGG
TTTGAAATTATCGAAGCTATCAACGGACAAGATATGCTAAATAAACTAGAGTCAATAATTAAGTTACCAGACATTATTTT
TACAGATATAAAAATGCCAATAATGGATGGAATTGAATTAACAAAACGTATCAAATCATCAGATAATACCAAACATATTC
CAGTTATAGCAATTACAGCCCATGGTATGGTGCATGAAGAGCTTGAAATTAAAAAACATTGTGATGGGTATCTTGCAAAA
CCAATAGAAAACAAAGAGTTAGACTCAATAATAACCACTATTTTTAAATAG

Upstream 100 bases:

>100_bases
GCAAACTATATTAGAAAAGCCTTTTTCAATAAAAACGTTAAAAAATACCGTAAAGAAAAATATTACCCTATACCATAATT
AAGAAAAGAAAGGACAACTA

Downstream 100 bases:

>100_bases
GCTCACTTGAGCCTATTTTCTTTTTTTCGTTTTAAAAATAGATATGCATTATTATTTTCAACGAATAAACCATCATCATC
ACTATAAAAAGACAGCATTG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 736; Mature: 736

Protein sequence:

>736_residues
MLLKNKFFVALAGTIIAYSVFISFTSYKIITSNQEEVKTIATNAENKYLHQSALHYSFDVSNSLNRKMIEIKNISKRYVE
LIKNIRSIKSRDILNTAVQELKKYSTSVIISDSKISYTTDETLVTQQYKINESTYLIFQTDVTKLLNRLKNKDYYADFVT
IIIDKNMNVVTDNSDSKQYLSLYKQKLKDITKLDEISYDLMIAMRKIEKKETWDSEIKINGEDHIIAIQPVKDLPWSIVT
LIKKKLSVNYDQDLTNKIDNNYILFSNKLFNTIIFITLFTLITSILITYYVKGPLILFSEWIKEIQRGNYEYNNPLLYRK
DELGTLARNVSLMAQDISILVNNLESKITERTKLLQQSKEQAEKANINKSKFIANISHELRTPLNAILGLSLYLIEKEEN
SEKKKTLETLNKAGQGLLEMVNDILDLSKIEADKIIAHNKPNSIDHIISDIIDITQFQSKSKNIQIQYKKTEFHHLNIDK
KYINKIIMNILSNAIKFTEDGGKIDIDVKYENSTPTTTDLIMVFTDNGRGIEEKDLERIFNPFEQLYDKDREYGTGLGLF
ITKKIIEDMNGNISIKSKVGIGTSICIKLKDVEIIKSKLIDQYHTMRRNVTKFKDSKTIAIIDDIEFNREVLKKKVEDYG
FEIIEAINGQDMLNKLESIIKLPDIIFTDIKMPIMDGIELTKRIKSSDNTKHIPVIAITAHGMVHEELEIKKHCDGYLAK
PIENKELDSIITTIFK

Sequences:

>Translated_736_residues
MLLKNKFFVALAGTIIAYSVFISFTSYKIITSNQEEVKTIATNAENKYLHQSALHYSFDVSNSLNRKMIEIKNISKRYVE
LIKNIRSIKSRDILNTAVQELKKYSTSVIISDSKISYTTDETLVTQQYKINESTYLIFQTDVTKLLNRLKNKDYYADFVT
IIIDKNMNVVTDNSDSKQYLSLYKQKLKDITKLDEISYDLMIAMRKIEKKETWDSEIKINGEDHIIAIQPVKDLPWSIVT
LIKKKLSVNYDQDLTNKIDNNYILFSNKLFNTIIFITLFTLITSILITYYVKGPLILFSEWIKEIQRGNYEYNNPLLYRK
DELGTLARNVSLMAQDISILVNNLESKITERTKLLQQSKEQAEKANINKSKFIANISHELRTPLNAILGLSLYLIEKEEN
SEKKKTLETLNKAGQGLLEMVNDILDLSKIEADKIIAHNKPNSIDHIISDIIDITQFQSKSKNIQIQYKKTEFHHLNIDK
KYINKIIMNILSNAIKFTEDGGKIDIDVKYENSTPTTTDLIMVFTDNGRGIEEKDLERIFNPFEQLYDKDREYGTGLGLF
ITKKIIEDMNGNISIKSKVGIGTSICIKLKDVEIIKSKLIDQYHTMRRNVTKFKDSKTIAIIDDIEFNREVLKKKVEDYG
FEIIEAINGQDMLNKLESIIKLPDIIFTDIKMPIMDGIELTKRIKSSDNTKHIPVIAITAHGMVHEELEIKKHCDGYLAK
PIENKELDSIITTIFK
>Mature_736_residues
MLLKNKFFVALAGTIIAYSVFISFTSYKIITSNQEEVKTIATNAENKYLHQSALHYSFDVSNSLNRKMIEIKNISKRYVE
LIKNIRSIKSRDILNTAVQELKKYSTSVIISDSKISYTTDETLVTQQYKINESTYLIFQTDVTKLLNRLKNKDYYADFVT
IIIDKNMNVVTDNSDSKQYLSLYKQKLKDITKLDEISYDLMIAMRKIEKKETWDSEIKINGEDHIIAIQPVKDLPWSIVT
LIKKKLSVNYDQDLTNKIDNNYILFSNKLFNTIIFITLFTLITSILITYYVKGPLILFSEWIKEIQRGNYEYNNPLLYRK
DELGTLARNVSLMAQDISILVNNLESKITERTKLLQQSKEQAEKANINKSKFIANISHELRTPLNAILGLSLYLIEKEEN
SEKKKTLETLNKAGQGLLEMVNDILDLSKIEADKIIAHNKPNSIDHIISDIIDITQFQSKSKNIQIQYKKTEFHHLNIDK
KYINKIIMNILSNAIKFTEDGGKIDIDVKYENSTPTTTDLIMVFTDNGRGIEEKDLERIFNPFEQLYDKDREYGTGLGLF
ITKKIIEDMNGNISIKSKVGIGTSICIKLKDVEIIKSKLIDQYHTMRRNVTKFKDSKTIAIIDDIEFNREVLKKKVEDYG
FEIIEAINGQDMLNKLESIIKLPDIIFTDIKMPIMDGIELTKRIKSSDNTKHIPVIAITAHGMVHEELEIKKHCDGYLAK
PIENKELDSIITTIFK

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788713, Length=385, Percent_Identity=29.6103896103896, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI48994928, Length=401, Percent_Identity=27.6807980049875, Blast_Score=156, Evalue=4e-39,
Organism=Escherichia coli, GI1789149, Length=330, Percent_Identity=29.6969696969697, Blast_Score=140, Evalue=3e-34,
Organism=Escherichia coli, GI87081816, Length=484, Percent_Identity=24.7933884297521, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI145693157, Length=263, Percent_Identity=33.0798479087452, Blast_Score=119, Evalue=6e-28,
Organism=Escherichia coli, GI1790436, Length=241, Percent_Identity=24.896265560166, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1786912, Length=259, Percent_Identity=23.5521235521236, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI1786600, Length=249, Percent_Identity=28.1124497991968, Blast_Score=77, Evalue=6e-15,
Organism=Escherichia coli, GI1788393, Length=274, Percent_Identity=25.9124087591241, Blast_Score=76, Evalue=7e-15,
Organism=Escherichia coli, GI1790346, Length=233, Percent_Identity=26.1802575107296, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI1788549, Length=247, Percent_Identity=25.1012145748988, Blast_Score=73, Evalue=8e-14,
Organism=Escherichia coli, GI1786783, Length=240, Percent_Identity=25, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI1787894, Length=217, Percent_Identity=24.8847926267281, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322044, Length=203, Percent_Identity=33.0049261083744, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 84950; Mature: 84950

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLKNKFFVALAGTIIAYSVFISFTSYKIITSNQEEVKTIATNAENKYLHQSALHYSFDV
CCCCCEEHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHCCCCHHHHHHHHHEEEEEC
SNSLNRKMIEIKNISKRYVELIKNIRSIKSRDILNTAVQELKKYSTSVIISDSKISYTTD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEECCC
ETLVTQQYKINESTYLIFQTDVTKLLNRLKNKDYYADFVTIIIDKNMNVVTDNSDSKQYL
CHHEEEEEEECCCEEEEEECCHHHHHHHHCCCCHHEEEEEEEEECCCEEEECCCCHHHHH
SLYKQKLKDITKLDEISYDLMIAMRKIEKKETWDSEIKINGEDHIIAIQPVKDLPWSIVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEECCCCCCHHHHH
LIKKKLSVNYDQDLTNKIDNNYILFSNKLFNTIIFITLFTLITSILITYYVKGPLILFSE
HHHHHHCCCCCHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
WIKEIQRGNYEYNNPLLYRKDELGTLARNVSLMAQDISILVNNLESKITERTKLLQQSKE
HHHHHHCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAEKANINKSKFIANISHELRTPLNAILGLSLYLIEKEENSEKKKTLETLNKAGQGLLEM
HHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
VNDILDLSKIEADKIIAHNKPNSIDHIISDIIDITQFQSKSKNIQIQYKKTEFHHLNIDK
HHHHHHHHHHCHHHHEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECEEEEECCCH
KYINKIIMNILSNAIKFTEDGGKIDIDVKYENSTPTTTDLIMVFTDNGRGIEEKDLERIF
HHHHHHHHHHHHHHHEECCCCCEEEEEEEECCCCCCCEEEEEEEECCCCCCCHHHHHHHH
NPFEQLYDKDREYGTGLGLFITKKIIEDMNGNISIKSKVGIGTSICIKLKDVEIIKSKLI
HHHHHHHHCCHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEECHHHHHHHHHH
DQYHTMRRNVTKFKDSKTIAIIDDIEFNREVLKKKVEDYGFEIIEAINGQDMLNKLESII
HHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
KLPDIIFTDIKMPIMDGIELTKRIKSSDNTKHIPVIAITAHGMVHEELEIKKHCDGYLAK
HCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCHHCC
PIENKELDSIITTIFK
CCCCCHHHHHHHHHCC
>Mature Secondary Structure
MLLKNKFFVALAGTIIAYSVFISFTSYKIITSNQEEVKTIATNAENKYLHQSALHYSFDV
CCCCCEEHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHCCCCHHHHHHHHHEEEEEC
SNSLNRKMIEIKNISKRYVELIKNIRSIKSRDILNTAVQELKKYSTSVIISDSKISYTTD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEECCC
ETLVTQQYKINESTYLIFQTDVTKLLNRLKNKDYYADFVTIIIDKNMNVVTDNSDSKQYL
CHHEEEEEEECCCEEEEEECCHHHHHHHHCCCCHHEEEEEEEEECCCEEEECCCCHHHHH
SLYKQKLKDITKLDEISYDLMIAMRKIEKKETWDSEIKINGEDHIIAIQPVKDLPWSIVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEECCCCCCHHHHH
LIKKKLSVNYDQDLTNKIDNNYILFSNKLFNTIIFITLFTLITSILITYYVKGPLILFSE
HHHHHHCCCCCHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
WIKEIQRGNYEYNNPLLYRKDELGTLARNVSLMAQDISILVNNLESKITERTKLLQQSKE
HHHHHHCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAEKANINKSKFIANISHELRTPLNAILGLSLYLIEKEENSEKKKTLETLNKAGQGLLEM
HHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
VNDILDLSKIEADKIIAHNKPNSIDHIISDIIDITQFQSKSKNIQIQYKKTEFHHLNIDK
HHHHHHHHHHCHHHHEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECEEEEECCCH
KYINKIIMNILSNAIKFTEDGGKIDIDVKYENSTPTTTDLIMVFTDNGRGIEEKDLERIF
HHHHHHHHHHHHHHHEECCCCCEEEEEEEECCCCCCCEEEEEEEECCCCCCCHHHHHHHH
NPFEQLYDKDREYGTGLGLFITKKIIEDMNGNISIKSKVGIGTSICIKLKDVEIIKSKLI
HHHHHHHHCCHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEECHHHHHHHHHH
DQYHTMRRNVTKFKDSKTIAIIDDIEFNREVLKKKVEDYGFEIIEAINGQDMLNKLESII
HHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
KLPDIIFTDIKMPIMDGIELTKRIKSSDNTKHIPVIAITAHGMVHEELEIKKHCDGYLAK
HCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCHHCC
PIENKELDSIITTIFK
CCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]