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The map label for this gene is pflB [H]

Identifier: 59713325

GI number: 59713325

Start: 157776

End: 160073

Strand: Reverse

Name: pflB [H]

Synonym: VF_A0142

Alternate gene names: 59713325

Gene position: 160073-157776 (Counterclockwise)

Preceding gene: 59713327

Following gene: 59713322

Centisome position: 12.03

GC content: 41.51

Gene sequence:

>2298_bases
ATGACTATTCCAACAACTGGTATGATAGAAGCATGGCGTGGCTTTACTAATGGCGAATGGCAAACGAGCGTAAACACTCG
TGATTTTATTCAAACAAACTACACTCCATATGAAGGAGATGAATCCTTCCTTGTAGAAGCAACAGAATCAACCACTACAT
TATGGAATAGCGTATTAGAAGGCATCAAACAAGAGAGCCGAACTCACGCTCCTGTTGATTTCGATACCGACCTACCTTCA
ACAATCATTTCTCATGACGCTGGTTACATCAATAAAGATCTAGAAAAAATCGTTGGTCTACAAACCGACAAACCCCTAAA
ACGCTCTATTATCGCTAACGGCGGTATTCGTATGGTGAAAAACTCATGCGAAGTTTATGGTCGTGAGTTAGATAAAAACG
TTGAGAAGATATTCACTGAATATCGCAAAACACACAACAAAGCATGTTTCGATCTTTATACTAAAGAGATCCTCGCATGT
CGTAAATCAGGCATCATCACGGGCCTTCCTGATGCTTATGGCCGTGGTCGTATCATTGGTGACTATCGTCGTTTAGCACT
TTACGGTATTGATTTCTTAAAAGCCGATAAAAAAGAGCAATACAACTCAACTCAAGCTTTCCTTGAACAAGGCCAAGATC
TTGAAAAAACCATGCGACTTCGTGAAGAGCTAGCAGACCAAGTTCAAGCACTAGAAGACATTCGTCAAATGGGCCTAAAA
TACGGTATTGATATGTCTCTACCAGCTAAAACAGCTCAAGAAGCCATTCAATTTACATATTTTGGTTACCTTGCTGCCGT
TAAATCTCAAAACGGTGCCGCAATGTCACTAGGCCGTACATCAACCTTCCTTGATGTTTATGTTGAACGTGATATTGCCA
ACGGTTTAATTACCGAAGAACAAGCACAAGAGATGATTGACCACTTCATCATGAAGTTACGTATGGTACGTTTCTTACGT
ACACCAGAATACGATTCACTTTTCTCTGGCGACCCAATCTGGGCAACAGAAGCCATGGCTGGTATGGGGGTTGATGGCCG
TACATTAGTGACTAAGACAACATTCCGTTATCTACACACATTGCACACAATGGGCCCAGCACCAGAGCCAAACATGACAA
TCCTTTGGTCAGAACAACTACCAATTGCCTTTAAGAAATACGCAGCGAAAGTGTCAATTGATACCTCATCAGTACAATAC
GAAAACGATGACTTAATGCGTCCTGATTTCGATAACGATGACTATGCCATTGCGTGTTGTGTAAGCCCACAAGTCGTTGG
TAAACACATGCAATTTTTTGGTGCTCGTGCCAACCTAGCAAAAGCTCTGCTTTACACCATCAATGGTGGTATTGATGAAA
AATCAAAAGCACAAGTTGGCCCACAAGTAGACAAAGTAAGCGACGAAGTCCTTGATTTCGACAGTCTAATGCCACGCTTT
GATTCAATGTTAGATTGGTTAGCAACACAATATGTGACGGCTTTAAATATCATTCACTATTCGCACGACCGTTACAGCTA
CGAAGCATCATTAATGGCATTAATGGATCGCGATGTTCGCCGAACAATGGCATGTGGTATTGCAGGTCTATCAGTGGTTG
CTGATTCATTAGCAGCCATTAAATTCGCTAAAGTAACTCCAGTTCGTGATGAAGATGGTGTGGCTATCGATTTCAACATT
GAAGGTGATTACCCTAAATTTGGTAATAATGATAACCGTGTTGATGACATTGCTTGTGATCTTGTTGAACGTTTCATGAA
AAAAATTCAAAAAATGAGCATGTACCGTGAAGCTATTCCAACACAATCGATTCTTACCATTACATCAAACGTTGTATACG
GTAAGAAAACAGGTAACACACCGGATGGCCGTCGTGCAGGCATGCCTTTTGGTCCGGGTGCAAACCCAATGCATGGTCGA
GATGAAAACGGAGCAGTAGCGTCGCTAACATCCGTATCAAAACTGCCATTTGCTTACGCTAAAGATGGTATTTCTTATAC
TTTCTCTATTGTGCCAAATGCACTTGGCAAAGATGAAGATGCACAAAAACAAAACCTTGCAGCACTTATGGATGGTTACT
TCCACCACGAAGCCGCAAACGCAAATGAGGGAATTGAAGGTGGCCAACATCTCAATGTTAACGTATTAAACCGTGATACA
TTGCTAGATGCTGTTGAACACCCAGAAAAATACCCACAATTAACCATTCGAGTATCAGGTTATGCTGTACGCTTTAACTC
ACTAACTCGTGAGCAACAGCAGGACGTAATTAGCCGTACATTTACAGAACAAATGTAG

Upstream 100 bases:

>100_bases
AAGAAAACAGTTTGTTATTACGGGTTATTGAACATCCTAATTCAAAAATACGATTCTACAAACTGATTGAAAAACAAATA
ATTAAAGATAGGTAAGCATT

Downstream 100 bases:

>100_bases
CAAGGGCTTTACTCAGTAAAATTAACGTCACCATTTAACTGGTGGCGTTAATTAAAAATAACCAACTGTAAATAAAGGAA
AAAAACAAAAAAATCCAATA

Product: formate acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase 1 [H]

Number of amino acids: Translated: 765; Mature: 764

Protein sequence:

>765_residues
MTIPTTGMIEAWRGFTNGEWQTSVNTRDFIQTNYTPYEGDESFLVEATESTTTLWNSVLEGIKQESRTHAPVDFDTDLPS
TIISHDAGYINKDLEKIVGLQTDKPLKRSIIANGGIRMVKNSCEVYGRELDKNVEKIFTEYRKTHNKACFDLYTKEILAC
RKSGIITGLPDAYGRGRIIGDYRRLALYGIDFLKADKKEQYNSTQAFLEQGQDLEKTMRLREELADQVQALEDIRQMGLK
YGIDMSLPAKTAQEAIQFTYFGYLAAVKSQNGAAMSLGRTSTFLDVYVERDIANGLITEEQAQEMIDHFIMKLRMVRFLR
TPEYDSLFSGDPIWATEAMAGMGVDGRTLVTKTTFRYLHTLHTMGPAPEPNMTILWSEQLPIAFKKYAAKVSIDTSSVQY
ENDDLMRPDFDNDDYAIACCVSPQVVGKHMQFFGARANLAKALLYTINGGIDEKSKAQVGPQVDKVSDEVLDFDSLMPRF
DSMLDWLATQYVTALNIIHYSHDRYSYEASLMALMDRDVRRTMACGIAGLSVVADSLAAIKFAKVTPVRDEDGVAIDFNI
EGDYPKFGNNDNRVDDIACDLVERFMKKIQKMSMYREAIPTQSILTITSNVVYGKKTGNTPDGRRAGMPFGPGANPMHGR
DENGAVASLTSVSKLPFAYAKDGISYTFSIVPNALGKDEDAQKQNLAALMDGYFHHEAANANEGIEGGQHLNVNVLNRDT
LLDAVEHPEKYPQLTIRVSGYAVRFNSLTREQQQDVISRTFTEQM

Sequences:

>Translated_765_residues
MTIPTTGMIEAWRGFTNGEWQTSVNTRDFIQTNYTPYEGDESFLVEATESTTTLWNSVLEGIKQESRTHAPVDFDTDLPS
TIISHDAGYINKDLEKIVGLQTDKPLKRSIIANGGIRMVKNSCEVYGRELDKNVEKIFTEYRKTHNKACFDLYTKEILAC
RKSGIITGLPDAYGRGRIIGDYRRLALYGIDFLKADKKEQYNSTQAFLEQGQDLEKTMRLREELADQVQALEDIRQMGLK
YGIDMSLPAKTAQEAIQFTYFGYLAAVKSQNGAAMSLGRTSTFLDVYVERDIANGLITEEQAQEMIDHFIMKLRMVRFLR
TPEYDSLFSGDPIWATEAMAGMGVDGRTLVTKTTFRYLHTLHTMGPAPEPNMTILWSEQLPIAFKKYAAKVSIDTSSVQY
ENDDLMRPDFDNDDYAIACCVSPQVVGKHMQFFGARANLAKALLYTINGGIDEKSKAQVGPQVDKVSDEVLDFDSLMPRF
DSMLDWLATQYVTALNIIHYSHDRYSYEASLMALMDRDVRRTMACGIAGLSVVADSLAAIKFAKVTPVRDEDGVAIDFNI
EGDYPKFGNNDNRVDDIACDLVERFMKKIQKMSMYREAIPTQSILTITSNVVYGKKTGNTPDGRRAGMPFGPGANPMHGR
DENGAVASLTSVSKLPFAYAKDGISYTFSIVPNALGKDEDAQKQNLAALMDGYFHHEAANANEGIEGGQHLNVNVLNRDT
LLDAVEHPEKYPQLTIRVSGYAVRFNSLTREQQQDVISRTFTEQM
>Mature_764_residues
TIPTTGMIEAWRGFTNGEWQTSVNTRDFIQTNYTPYEGDESFLVEATESTTTLWNSVLEGIKQESRTHAPVDFDTDLPST
IISHDAGYINKDLEKIVGLQTDKPLKRSIIANGGIRMVKNSCEVYGRELDKNVEKIFTEYRKTHNKACFDLYTKEILACR
KSGIITGLPDAYGRGRIIGDYRRLALYGIDFLKADKKEQYNSTQAFLEQGQDLEKTMRLREELADQVQALEDIRQMGLKY
GIDMSLPAKTAQEAIQFTYFGYLAAVKSQNGAAMSLGRTSTFLDVYVERDIANGLITEEQAQEMIDHFIMKLRMVRFLRT
PEYDSLFSGDPIWATEAMAGMGVDGRTLVTKTTFRYLHTLHTMGPAPEPNMTILWSEQLPIAFKKYAAKVSIDTSSVQYE
NDDLMRPDFDNDDYAIACCVSPQVVGKHMQFFGARANLAKALLYTINGGIDEKSKAQVGPQVDKVSDEVLDFDSLMPRFD
SMLDWLATQYVTALNIIHYSHDRYSYEASLMALMDRDVRRTMACGIAGLSVVADSLAAIKFAKVTPVRDEDGVAIDFNIE
GDYPKFGNNDNRVDDIACDLVERFMKKIQKMSMYREAIPTQSILTITSNVVYGKKTGNTPDGRRAGMPFGPGANPMHGRD
ENGAVASLTSVSKLPFAYAKDGISYTFSIVPNALGKDEDAQKQNLAALMDGYFHHEAANANEGIEGGQHLNVNVLNRDTL
LDAVEHPEKYPQLTIRVSGYAVRFNSLTREQQQDVISRTFTEQM

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=755, Percent_Identity=76.0264900662252, Blast_Score=1241, Evalue=0.0,
Organism=Escherichia coli, GI48994926, Length=756, Percent_Identity=71.1640211640212, Blast_Score=1173, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=688, Percent_Identity=25, Blast_Score=173, Evalue=3e-44,
Organism=Escherichia coli, GI1790388, Length=688, Percent_Identity=23.9825581395349, Blast_Score=148, Evalue=2e-36,
Organism=Escherichia coli, GI1788933, Length=61, Percent_Identity=83.6065573770492, Blast_Score=111, Evalue=2e-25,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 85739; Mature: 85608

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2 ; PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIPTTGMIEAWRGFTNGEWQTSVNTRDFIQTNYTPYEGDESFLVEATESTTTLWNSVLE
CCCCCCCHHHHHCCCCCCCEEECCCCHHHHCCCCCCCCCCCCEEEEECCHHHHHHHHHHH
GIKQESRTHAPVDFDTDLPSTIISHDAGYINKDLEKIVGLQTDKPLKRSIIANGGIRMVK
HHHHHCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHH
NSCEVYGRELDKNVEKIFTEYRKTHNKACFDLYTKEILACRKSGIITGLPDAYGRGRIIG
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEECCCCCCCCCCEEH
DYRRLALYGIDFLKADKKEQYNSTQAFLEQGQDLEKTMRLREELADQVQALEDIRQMGLK
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGIDMSLPAKTAQEAIQFTYFGYLAAVKSQNGAAMSLGRTSTFLDVYVERDIANGLITEE
HCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHEEHHHHHCCCCCHH
QAQEMIDHFIMKLRMVRFLRTPEYDSLFSGDPIWATEAMAGMGVDGRTLVTKTTFRYLHT
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCCEEHHHHHHHHHHH
LHTMGPAPEPNMTILWSEQLPIAFKKYAAKVSIDTSSVQYENDDLMRPDFDNDDYAIACC
HHHCCCCCCCCEEEEECCCCCHHHHHHHHEEEEECCCEEECCCCCCCCCCCCCCCEEEEE
VSPQVVGKHMQFFGARANLAKALLYTINGGIDEKSKAQVGPQVDKVSDEVLDFDSLMPRF
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHH
DSMLDWLATQYVTALNIIHYSHDRYSYEASLMALMDRDVRRTMACGIAGLSVVADSLAAI
HHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KFAKVTPVRDEDGVAIDFNIEGDYPKFGNNDNRVDDIACDLVERFMKKIQKMSMYREAIP
EEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
TQSILTITSNVVYGKKTGNTPDGRRAGMPFGPGANPMHGRDENGAVASLTSVSKLPFAYA
CCHHHEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHCCHHHH
KDGISYTFSIVPNALGKDEDAQKQNLAALMDGYFHHEAANANEGIEGGQHLNVNVLNRDT
CCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECHHH
LLDAVEHPEKYPQLTIRVSGYAVRFNSLTREQQQDVISRTFTEQM
HHHHHHCCCCCCEEEEEEECEEEEEHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TIPTTGMIEAWRGFTNGEWQTSVNTRDFIQTNYTPYEGDESFLVEATESTTTLWNSVLE
CCCCCCHHHHHCCCCCCCEEECCCCHHHHCCCCCCCCCCCCEEEEECCHHHHHHHHHHH
GIKQESRTHAPVDFDTDLPSTIISHDAGYINKDLEKIVGLQTDKPLKRSIIANGGIRMVK
HHHHHCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHH
NSCEVYGRELDKNVEKIFTEYRKTHNKACFDLYTKEILACRKSGIITGLPDAYGRGRIIG
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEECCCCCCCCCCEEH
DYRRLALYGIDFLKADKKEQYNSTQAFLEQGQDLEKTMRLREELADQVQALEDIRQMGLK
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGIDMSLPAKTAQEAIQFTYFGYLAAVKSQNGAAMSLGRTSTFLDVYVERDIANGLITEE
HCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHEEHHHHHCCCCCHH
QAQEMIDHFIMKLRMVRFLRTPEYDSLFSGDPIWATEAMAGMGVDGRTLVTKTTFRYLHT
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCCEEHHHHHHHHHHH
LHTMGPAPEPNMTILWSEQLPIAFKKYAAKVSIDTSSVQYENDDLMRPDFDNDDYAIACC
HHHCCCCCCCCEEEEECCCCCHHHHHHHHEEEEECCCEEECCCCCCCCCCCCCCCEEEEE
VSPQVVGKHMQFFGARANLAKALLYTINGGIDEKSKAQVGPQVDKVSDEVLDFDSLMPRF
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHH
DSMLDWLATQYVTALNIIHYSHDRYSYEASLMALMDRDVRRTMACGIAGLSVVADSLAAI
HHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KFAKVTPVRDEDGVAIDFNIEGDYPKFGNNDNRVDDIACDLVERFMKKIQKMSMYREAIP
EEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
TQSILTITSNVVYGKKTGNTPDGRRAGMPFGPGANPMHGRDENGAVASLTSVSKLPFAYA
CCHHHEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHCCHHHH
KDGISYTFSIVPNALGKDEDAQKQNLAALMDGYFHHEAANANEGIEGGQHLNVNVLNRDT
CCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECHHH
LLDAVEHPEKYPQLTIRVSGYAVRFNSLTREQQQDVISRTFTEQM
HHHHHHCCCCCCEEEEEEECEEEEEHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3053170; 8905232; 9278503; 2651404; 1310545; 9629924; 10504733 [H]